• Korean Journal of Pharmacognosy
• /
• v.2 no.4
• /
• pp.163-172
• /
• 1971

This paper attempts to observe the histological structure of Korean Arisaema ringens $S_{CHOTT}{'s}$ rhizome, to identify it's constituent and to investigate the pharmacological action with it's alcohol extract. The results are : 1) The inner structure of rhizome on the transverse section is divided into external and internal tissues by the ring of intercellular secretary sac. Raphides of calcium oxalate contained in mucilage cell, collateral vascular bundle, are extremely similar to those contained in Pinellia ternata. 2) The organs of the pistillate Arisaema ringens are larger and more plentiful than those of the staminate Arisaema ringens. The sexual identification is easy in the flowering season. 3) The alkaloid is identified by Meyer reagent as white p.p.t. at pH 2 of sulfuric acid. 4) The saponin is indentified remarkably by means of foaming reaction, Lieberman-Burchard reaction and hemolytic reaction. 5) The effect of alcohol extract on the relaxation of the isolated intestine of the rabbit is remarkably shown at the concentration of $10^{-3}g/ml$. 6) The effect of alcohol extract on the isolated ractus muscle of the frog increases the constructive action of acetylcholine at the concentration of $10^{-3}g/ml$. 7) The effect of alcohol extract on the isolated heart movement of the frog is decreased remarkably at the concentration of $10^{-3}g/ml$. 8) The effect of alcohol extract on the blood pressure of the rabbit is decreased by an interavenous injection of $10^{-3}g/kg$.

• The Korea Journal of Herbology
• /
• v.29 no.6
• /
• pp.35-43
• /
• 2014

Objectives : Official Arisaematis Rhizoma is described only three species, Arisaema amurnse, Arisaema erubescens, and Arisaema heterophyllum, in national Pharmacopoeia. However, other Arisaema species, Arisaema ringens, Arisaema takesimense and Arisaema serratum, also have been distributed as an inauthentic Arisaematis Rhizoma in the herbal market. To develop a reliable molecular authentication method for Arisaematis Rhizoma in species level, we analyzed DNA barcode regions using six Arisaema species. Methods : Thirty-eight samples of six Arisaema plants species (A. amurense, A. amurense f. serratum, A. heterophyllum, A. takesimense, and A. serratum) were collected from different habitate and nucleotide sequences of DNA barcode regions (rDNA-ITS, matK, and rbcL gene) were analyzed after PCR amplification. The species-specific sequences and phylogenetic relations were estimated using entire sequences of three DNA barcodes based on the analysis of ClastalW and UPGMA, respectively. Results : The comparative analysis of DNA barcode sequences were revealed inter-species specific nucleotides to distinguish the medicinal plant of Arisaema Rhizoma in species levels excluding between A. amurense and its subspecies (A. amurense f. serratum) and A. takesimense and A. serratum, respectively. However, we obtained sequence differences enough to discriminate authentic and inauthentic Arisaematis Rhizoma. Therefore, we suggest that these SNP type molecular genetic markers were an reliable method avaliable to identify official herbal medicines. Conclusions : These marker nucleotides could be useful to identify the official herbal medicines by providing definitive information that can identify original medicinal plant and distinguish from inauthentic adulterants and substitutes.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.10 no.1
• /
• pp.16-22
• /
• 2007

This study was carried out to investigate the effects of shading conditions for appropriate light level for several floricultural plants native to Korea. The results under the different shading levels of 0, 30, 50, 60% and full sun light are as follows; Growth of Miscanthus sinensis and Pennisetum alopecuroides, which are heliophyte plants, were better under 30% shading condition than full sunlight. Higher shading levels was poor than control. Growth of Sedum spectable was decreased when light intensity was decreased. As shading level increased, growth reduction rate in Sedum spectable were higher than that of other species. Growth and ornamental value of Arisaema amurense, which is sciophyte plants, were increased under 50% shading.

• Korean Journal of Plant Resources
• /
• v.31 no.1
• /
• pp.37-51
• /
• 2018

Molecular phylogenetic analysis was conducted to evaluate the taxonomic relationships of genus Arisaema L. distributed in Korea and the molecular phylogenetic characteristics of three authentic Arisaema species for the herbal medicine Arisaematis Rhizoma (the rhizomes of A. amurense, A. heterophyllum, and A. erubescens). The sequences of three DNA barcodes (rDNA-ITS, matK, and rbcL) were analyzed using 50 samples of nine taxa consisted of eight Korean and one Chinese Arisaema with one outgroup (Dracunculus vulgaris). Both individual and combined phylogenetic analyses of three DNA barcode sequences revealed that the treated nine taxa are independently classified into six distinct clades (Clade I, A. amurense f. amurense and A. amurense f. serratum; Clade II, A. serratum and A. takesimense; Clade III, A. ringens; Clade IV, A. erubescens; Clade V, A. heterophyllum; Clade VI, A. thunbergii subsp. thunbergii and A. thunbergii subsp. geomundoense). These six clades were reasonably divided into three individual sections, Pedatisecta, Sinarisaema, and Tortuosa. Futhermore, the results of comparative DNA barcode sequences analyses provided a significant information for the taxonomic reconsideration of Arisaema L. at the specific and intraspecific level. However, we could not confirm the taxonomic characteristics or identity among the three authentic medicinal species through the molecular phylogenetic analyses of genus Arisaema L. for Arisaematis Rhizoma.

• Asian-Australasian Journal of Animal Sciences
• /
• v.29 no.9
• /
• pp.1280-1286
• /
• 2016

This study was aimed to evaluate the in vitro effects of medicinal herb extracts (MHEs) on ruminal fermentation characteristics and the inhibition of protozoa to reduce methane production in the rumen. A fistulated Hanwoo was used as a donor of rumen fluid. The MHEs (T1, Veratrum patulum; T2, Iris ensata var. spontanea; T3, Arisaema ringens; T4, Carduus crispus; T5, Pueraria thunbergiana) were added to the in vitro fermentation bottles containing the rumen fluid and medium. Total volatile fatty acid (tVFA), total gas production, gas profiles, and the ruminal microbe communities were measured. The tVFA concentration was increased or decreased as compared to the control, and there was a significant (p<0.05) difference after 24 h incubation. pH and ruminal disappearance of dry matter did not show significant difference. As the in vitro ruminal fermentation progressed, total gas production in added MHEs was increased, while the methane production was decreased compared to the control. In particular, Arisaema ringens extract led to decrease methane production by more than 43%. In addition, the result of real-time polymerase chain reaction indicted that the protozoa population in all added MHEs decreased more than that of the control. In conclusion, the results of this study indicated that MHEs could have properties that decrease ruminal methanogenesis by inhibiting protozoa species and might be promising feed additives for ruminants.

• Journal of the Korean Institute of Traditional Landscape Architecture
• /
• v.31 no.3
• /
• pp.54-60
• /
• 2013

This study carried out a phytosociological research by Z.-M. school's method describing vegetation structure and management solution about nutmeg tree's(Torreya nucifera ) forests. The nutmeg tree's forests have developed intervale and hills in warm-temperate evergreen broad-leaved forests zone. The nutmeg tree's forests composed 50 familly, 80 genus, 88 species divided two syntaxa(typical sub-community, Arachniodes aristata sub-community) by species composition and correspond to habitat condition. The nutmeg tree's forests show a uniform forest's structure which composed 3~4 layer of sub-tree and shrub. And it was worthy of special mention which characterized diagnostic species of Camellietea japonicae in the warm-temperate evergreen broad-leaved forests. In case of forests floor, were a high rate in order of Ophiopogon japonicus, Polystichum tripteron, Arisaema ringens. And Oplismenus undulatifolius which represent mantle communities as well. This indicates that the nutmeg tree's forests are exposed by humman effects sustainedly. The nutmeg tree's young trees appeared a high rate at half shade and shade. The vigorous tree species have to spacing cutting instead of the heavy thinning that consist in with a shout of declining astructur and function of the forest ecosystem for maintaining a nutmeg tree's forests. And we suggest for the density regulation of the sub-tree and shrub. In conclusion, need to the continuous monitoring about the vegetation structure and management solution for the sustainable nutmeg tree's forests.

• Journal of Korean Society of Forest Science
• /
• v.107 no.3
• /
• pp.245-257
• /
• 2018

The study was carried out to discover the forest vegetation structure in Hongdo Island, Jeonnam province. Vegetation data were collected by total of forty one quadrate plots using Z-M phytosociological method from June to August in 2017, and analyzed by vegetation classification, mean importance value and species diversity. As a result of vegetation type classification, Castanopsis sieboldii community group was classified at a top level of vegetation hierarchy. In the level of community, it was classified into Neolitsea sericea community and Carpinus turczaninowii community. N. sericea community was subdivided into Ficus erecta group(Vegetation unit 1) and Arisaema ringens group(VU 2). C. turczaninowii community was subdivided into Fraxinus sieboldiana group(VU 3) and C. turczaninowii typical group(VU 4). Therefore, it was classified into total of four vegetation units(one community group, three communities and four groups). As a result of mean importance value, Castanopsis sieboldii was the highest in VU 1, VU 2, VU 4, and C. turczaninowii in VU 4, respectively. In case of species diversity, VU 3 showed the highest among four units in species diversity index. In conclusion, the forest vegetation of Hongdo Island was classified into four units and seven species groups. Hongdo Island could be conclusively managed by community ecological approach for the units and groups. Also it was considered that a research for the succession to the evergreen broad-leaved forest should be more intensively proceeded near future.

• Korean Journal of Plant Resources
• /
• v.21 no.2
• /
• pp.117-127
• /
• 2008

The vascular plants in the Eco-Arboretum site, Mt. Gumwon appeared to be 369 taxa; 84 families, 217 genera, 319 species, 43 varieties and 7 forms. Based on the list of the rare plants, 2 taxa existed in the studied areas; Abies koreana (Preservation priority order; No. 73), Paeonia obovata (No. 97) and based on the list of Korean endemic plants, 12 families, 14 genera, 11 species, 3 varieties, 1 hybrid, 15 taxa existed; Cephalotaxus koreana, Abies koreana (Planted species), Carex okamotoi, Salix hallaisanensis, Salix purpurea var. japonica, Clematis trichotoma, Thalictrum actaefolium, Lespedeza ${\times}$ tomentella, Vicia nipponica, Stewartia koreana, Hypericum attenuatum var. confertissimum, Paulownia coreana (Planted species), Weigela subsessilis, Aster koraiensis (Planted species). Specific plant species by floral region were total 38 taxa; Paeonia obovata in class V , 13 taxa (Lilium leichtlinii var. tigrinum, Betula costata, Betula davurica, Ulmus davidiana, Spiraea frutschiana, Prunus maackii, Syringa reticulata var. mandshurica, Cynanchum inamoenum, Lonicera subhispida, Cacalia firma, etc.) in class III , 5 taxa (Heloniopsis orientalis, Chrysosplenium pilosum, Acer triflorm, Viola tokubuchiana var. takedana, Teucrium veronicoides, etc.) in Class II . 19 taxa (Dryopteris erythrosora, Arisaema ringens, Veratrum versicolor, Polygonatum inflatum, Salix glandulosa, Betula schmidtii, Alnus hirsuta, Quercus variabilis, Aconitum japuense, Dicentra spectabilis, Chrysosplenium flagelliferum, Impatiens noli-tangere, Ilex macropoda, Tilia amurensis, Fraxinus mandshurica, Trigonotis icumae, etc.)in class I . The naturalized plants in this site were 5 families, 13 genera, 15 taxa (Rumex acetocella, Rumex obtusifolius, Trifolium repens, Oenothera odorata, Erigeron annuus, Erigeron philadelphicus, Bidens frondosa, Taraxacum officinale, etc.) and naturalization rate was 4.1% of all 369 taxa vascular plants.