• The Korean Journal of Mycology
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• v.41 no.2
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• pp.81-94
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• 2013

The purpose of this study was to investigate effects of inoculation of arbuscular mycorrhizal fungi (AMF) on growth of Korean ginseng (Panax ginseng C. A. Mey.) seedlings. Five species of AMF (Glomus mosseae, Glomus intraradices, Glomus etunicatum, Acaulospora longula, Acaulospora trappei) were inoculated to ginseng seedlings at 60 days after ginseng seed germination. Dry weights, heights of the plants and chlorophyll fluorescence were measured at 120 days after inoculation of AMF. Dry weights and shoot heights were increased in seedlings inoculated with AMF compared to the controls without AMF. However, chlorophyll fluorescence of seedlings with AMF showed no significant difference compared to the control.

• The Korean Journal of Mycology
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• v.40 no.1
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• pp.1-6
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• 2012

In this study, soil samples were collected from cultivated fields of 1-5 year old Korean ginseng in Geumsan, Korea. Spores of arbuscular mycorrhizal fungi were extracted from soils and identified using morphological characteristics and 18s rDNA sequences of the spores. Total 10 species of AMF were identified: Acaulospora longula, Archaeospora trappei, Glomus caledonium, Glomus etunicatum, Glomus intraradices, Glomus mosseae, Glomus sp., Paraglomus occultum, Paraglomus brasilianum, and Scutellospora heterogama. Relative abundance of spores of A. trappei were increased with increase of cultivation period of the ginseng. However, relative abundance of other species of AMF and Shannon diversity (H') of AMF were significantly decreased with the increase of cultivation periods of the ginseng.

• Journal of Ginseng Research
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• v.30 no.4
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• pp.206-211
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• 2006

To investigate mycorrhizal infection by arbuscular mycorrhizal fungi(AMF), samples of fine lateral roots were taken from the wild ginseng(Panax ginseng C.A. Meyer) naturally growing at various locations in Korea. Mycorrhiazal infections were studied by cleaning the root samples and staining fungal hyphae with frypan blue. Wild ginsengs for this study were graded by an appraisal committee consisting of 12 experts of Korea Mountain Ginseng Association. Following five quality groups were recognized: Heaven group(pure natural), Earth group (from seeding of wild ginseng), Man group(from seeding or seedlings of wild ginseng with slight environmental modification), unmarketable, and imported wild ginseng. Morphology of AMF was typical Paris-type which shows intracellular hyphal coils with rare vesicles and lack of arbuscules. Average infection rate of individual wild ginsengs was 58.3% and showed no differences among five quality groups. When portions of fine roots were quantified for mycorrhizal infection, 18.7% of the total length of the primary and secondary roots were infected by AMF. Wild ginsengs from Gyeonggi Province(84.2%), and from mountains lower than 1,200 meters above sea level(about 70%) showed higher infection rate, while the ginseng from Gyeongbuk Province(27.8%) had lower rate. Wild ginsengs at older age showed lower infection rates.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.5
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• pp.341-349
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• 2004

This study was conducted to compare the effects of arbuscular mycorrhizal fungi (AMF) on the growth and fruit yield of hydroponically grown cucumber and tomato plants in solid medium culture. Mycorrhizal fungus Glomus sp. was collected from plastic film house soils of cucumber and tomato and inoculated to the experimental crops at the time of seeding and transplanting. Root infection of cucumber and tomato plants by AMF was more significantly increased when the AMF was inoculated at seeding stage than at transplanting stage. In the infected roots of cucumber and tomato, mycorrhizal hyphae was easily observed but vesicle and arbuscule were rare. Overall plant growth was increased with AMF inoculation and the growth was higher when AMF was inoculated at seeding stage. Fresh weight of each fruit of cucumber and tomato and sugar content in tomato fruits were significantly increased with AMF inoculation at seeding stage. The AMF inoculation also increased fruit yields of cucumber and tomato.

• The Korean Journal of Mycology
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• v.42 no.4
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• pp.306-311
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• 2014

In this study, arbuscular mycorrhizal fungi (AMF) were isolated and cultivated from rhizospheres of various plants in Korea. Five species belonging to four genus were identified based on spore morphology and 18S rDNA sequence analysis: Acaulospora cavernata Claroideoglomus luteum, Diversispora aurantium, D. trimurales, Rhizophagus irregularis. These fungal species have not been cultivated or reported to date in Korea, Morphological description and phylogenetic analysis for each species were presented. This result could be useful for research of AMF diversity in Korea.

• Mycobiology
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• v.51 no.3
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• pp.164-168
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• 2023

This study examined the effects of myristate on an asymbiotic culture of Rhizoglomus intraradices, a species of arbuscular mycorrhizal fungi (AMF; Glomeromycota). Mycelial growth and sporulation in a modified medium containing myristate were observed. The findings demonstrated that myristate induced R. intraradices spore formation, with daughter spores having a smaller diameter than the parent spores. This observation is consistent with previous studies on other Rhizoglomus species. Further studies are needed to investigate the potential for continuous culture, mass production using daughter spores, and the application of AMF colonization techniques in plants.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.1
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• pp.68-75
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• 1999

This study was conducted to examine the effects of Arbuscular Mycorrhizae inoculation and phosphorus application on early growth of hot pepper. Gigaspora margarita and Acaulospora spinosa were chosen for this investigation and inoculated into soils of different P levels by varying inoculation time and density. After treatment, some relevant growth responses of hot pepper were measured. Regardless of soil P levels, hot peppers treated with arbuscular mycorrhizal fungi had 5~34% more fresh weight than those untreated, but the effect of inoculation time and density was not different between two species. With decreased P levels, the infection rate and dependency of hot peppers increased. The content of P and K of AMF-inoculated hot peppers increased with increasing P levels, but the shoot to root ratio of those elements decreased. The results of this study showed that inoculation of AMF would be effective in promoting growth of hot pepper seedlings and increase transplant adaptation due in part to the resulted higher root development.

• Korean Journal of Plant Resources
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• v.27 no.2
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• pp.194-201
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• 2014

This study was carried out to investigate the characteristics and infection effects of AMF with soil samples collected at some sites in Gyeonggi province, Korea. AMF spore and characteristics of infection structure in upland plant root were observed as wet sieving and staining method, growth of Capsicicum annum was compared between treatment and non treatment. AMF spores isolated from each soil sample were ellipse or circle type and the colors were soft yellow and white. The colonization rate of AMF with soil infection of Zea mays roots was 13.3~83.3%, the rate of infected soil collected from Z. mays was higher on average. When compared to the growth of C. Annum with control after infection on C. annum, it wasn't showing many differences in fresh weight, dry weight and height, but the yield of fruit of C. annuum showed double than the control group.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.3
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• pp.170-176
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• 2008

This study was carried out to improve density of arbuscular mycorrhizal fungi (AMF) propagule and physiochemical properties of soil by planting crops at the preplanning field for ginseng cultivation. Winter crops, such as barley and rye and summer crops, such as sudangrass and soybean were cultivated in combination to improve AMF propagation and soil aggregation at the fields. Yield of harvested crops by plating with winter or/and summer crops was $3,045kg\;10a^{-1}$ of the only rye cultivation, $2,757kg\;10a^{-1}$ of sudangrass cultivation in combination with rye growing (rye/sudangrass) and $1,628kg\;10a^{-1}$ of soybean cultivation in combination with barley growing (barley/soybean), respectively. Soil aggregation rate was improved by cultivation with barley (45.7%) and with rye/sudangrass (45.1%), respectively. The density of AMF spores in soil was increased slowly by cultivating with winter crops. In summer crops cultivation system, density of AMF spores at sudangrass cultivated field was $64.0spores\;g^{-1}$ dried soil and it was higher than that at soybean cultivated field. External hyphae length (EHL) was $1.5{\sim}2.0m\;g^{-1}$ air-dried soil at winter crops cultivated field. However, in summer crops cultivation systems, EHL was $2.6{\sim}2.9m\;g^{-1}$ airdried soil at sudangrass cultivated field and was $1.7{\sim}2.2m\;g^{-1}$ air-dried soil at soybean cultivated filed, showing these were higher than those in non-cultivated field (control). Glomalin content of soil cultivated with crops was higher than that of control soil. Especially, the highest glomalin content was shown to $1.7m\;g^{-1}$ air-dried soil in the barley/soybean cultivation systems. These results suggested that the most effective soil management to improve AMF propagule density and soil physical properties by planting crops system was cultivating sudangrass followed by barley at the preplanning fields for ginseng cultivation.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.5
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• pp.373-378
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• 2013

Information on the effective application method of arbuscular mycorrhizal fungi (AMF) inoculum is still inadequate. This work was performed to assess two AMF inoculation methods (dispersed and proximate) on root colonization of sorghum-sudangrass hybrid (Sorghum bicolor L.). In dispersed inoculation method, spores were inoculated in 2 kg pots of soil in which 5 day-old seedlings were transplanted and maintained for 50 days. In the proximate inoculation method, spores were first introduced in 500 mL pots where seeds were sown. After 10 days, the seedlings with the 500 mL soil were transferred to 2 kg pots without disturbing the contents. After 50 days of growth, root colonization and arbuscule abundance significantly increased (over 100%) in proximate method of inoculation. Moreover, sorghum-sudangrass hybrid had higher shoot growth (182.5 cm) and Glomalin related soil protein (GRSP) production in proximate method. Nutrient accumulation, particularly total nitrogen (82.61 mg $plant^{-1}$), was also found to be higher in proximate method of inoculation. Our results demonstrate that the proximate method of inoculation may improve the early stage mycorrhizal symbiosis and inoculum performance in Saemangeum reclaimed soil.