• Korean Journal of Medicinal Crop Science
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• v.13 no.4
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• pp.182-185
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• 2005

Bioassay-guided isolation of the dried roots of Aralia continentalis led to the isolation of (-)-pimara-8(14), 15dien-19-oic acid (continentalic acid) and $(24E)-Stigmasta-5,22-dien-3{\beta}-ol$ (stagmasterol). Their structures were elucidated using $^1H-NMR$, $^{13}C-NMR$, UV and mass spectra analyses. The gram-positive bacterial, including methicilline-resistant (MRSA), were more sensitive to the continentalic acid and stagmasterol than gram-negative bacterial.

• Korean Journal of Medicinal Crop Science
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• v.15 no.6
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• pp.371-390
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• 2007

Arthropod pests were surveyed from 132 herb species of 121 genera in 50 families of 32 orders at herb garden of Sancheong-gun Agricultural Development Technology Center in Sancheong, Gyeongsangnamdo province from October, 2005 to November, 2006. Ninety eight arthropod pests of 86 genera in 44 families of 9 orders were collected. Although less than 5 arthropod pests were collected from each herb, more than 5 arthropod pests were occurred on Hibiscus mutabililis, Peucedanum japonicum, Aralia cordata, Fraxinus rhynchophylla, Angelica decursiva, Rhaponticum uniflorum, Lonicera japonica, Betula platyphylla and Agrimonia pilosa. Out of collected pests, 98.5% of them damaged leaves of medicinal plants. The highest number of arthropod pests was recorded in May representing 36 species of 32 genera in 20 families of 6 orders. Most of them were collected from less than 5 medicinal plants. However, Atractomorpha lata, Dolycoris baccarum, Myzus persicae, and Nysius plebejus occurred on 42., 22, 20, and 15 medicinal plants, respectively. Atractomorpha lata and Myzus persicae had broad host ranges and seriously damaged. Aphis gossypii was dominant insect pest in May compared with Dolycoris baccarum in June. Atractomorpha lata in August and September, and Myzus persicae in October.

• Journal of Sasang Constitutional Medicine
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• v.34 no.4
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• pp.1-12
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• 2022

Objectives This paper investigates the origins, changes, and the constructive principles of Hyungbangjihwang-tang(荊防地黃湯). Methods Hyungbangjihwang-tang and other related prescriptions were analyzed in terms of their pathological indications, based on previous literature including 『Gogeumuigam(古今醫鑑)』, 『Sanghannon(傷寒論)』, 『Uihagjeongjeon(醫學正傳)』, 『Donguisusebowon·Sasangchobongwon(東醫壽世保元·四象草本卷)』, 『Donguisusebowon·Gabobon(東醫壽世保元·甲午本)』, 『Donguisusebowon·Sinchukbon(東醫壽世保元·辛丑本)』. Results and Conclusions The origins of Hyungbangjihwang-tang can be seen in three sections depending on the classification of medicinal herbs. Schizonepeta tenuifolia Briq.(荊芥), Saposhnikovia divaricata Schischk.(防風), Notopterygium incisum Ting(羌活), Aralia cordata Thunb.(獨活), and Plantago asiatica L.(車前子) originated from Hyungbangpaedok-san(荊防敗毒散) included in 『Gogeumuigam』. Poria cocos Wolf.(茯苓) and Alisma canaliculatum(澤瀉) originated from Ohryoung-san(五苓散) included in 『Sanghannon』. Rehmannia glutinosa for hueichingensis(熟地黃) and Cornus officinalis S. et Z.(⼭茱萸) originated from Yukmijihwang-tang(六味地黃湯) included in 『Uihagjeongjeon』. Hyungbangpaedok-san was transformed into "Paedok-san" in 『Chobongwon(草本卷)』, Hyungbangpaedok-san in the 『Gabobon(甲午本)』 and 『Sinchukbon(辛丑本)』. Yukmijihwang-tang was mentioned as a Soyangin medicine in the 『Chobongwon』 and was later transformed into a Suhwagije-tang(水火旣濟湯) in 『Gabobon』. The meaning of Yukmijihwang-tang inherited in 『Sinchukbon』 as Dokhwaljihwang-tang and Hyungbangjihwang-tang. The medicinal herbs in Hyungbangjihwang-tang can be categorized in three sections by their functions, Schizonepeta tenuifolia Briq., Saposhnikovia divaricata Schischk., Notopterygium incisum Ting, Aralia cordata Thunb., and Plantago asiatica L. scatter the Pyohan(表寒) and make the Pyoeum(表陰) drop. Poria cocos Wolf.(茯苓) and Alisma canaliculatum(澤瀉) make the Pyoeum(表陰) drop through Lee-su(利水). Rehmannia glutinosa for hueichingensis(熟地黃) and Cornus officinalis S. et Z.(⼭茱萸) tonify Shin-won(腎元). The each principles of medicinal herbs corresponds to the stage of the "Cold-related Diarrhea in Soyangin Spleen Cold-based Exterior Cold disease(少陽人 脾受寒表寒病 亡陰病 身寒腹痛亡陰證)".

• Proceedings of the Botanical Society of Korea Conference
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• 1993.07a
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• pp.1-36
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• 1993

It is possible to regenerate plants from calli, single cells and protoplasts of numerous species via organogenasis or embryogenesis in cell and tissue culture systems. Also such regeneration of plants can directly occur from cells of explants. However certain plant species has not been yet provided cultures suitable for plant regeneration from cells or tissues. For example, we have to confirm the regenerability of plant from cells before preparing transformed cells for application. Even more, it is very important to notice that regenerated plants in cell and tissue cultures often show structural abnormality. The mojority of those plants is functionally disordered and eventually cases degenerated. One of such examples is vitreous plants which are manifested mainly in the leaves and manifesteds to a lesser extent in the stems and roots. Regenerants in suspension cultures show more frequent vitrification than on gelled media so that relative humidity and water potential are the key factors involved in abnormal morphogenesis in vitro. The other is that somatic embryos formed in media containing BAP or high concentration of sucrose show frequently cotyledon aberrancy such as polycotyledon and born type cotyledon. The embryos with aberrant cotyledon of Codonopsis lanceolata could not germinate or regenerate into plants in many cases. In contrast, the polycotyledon embryos of Aralia cordata germinated in higher percentage than two cotyledonary embryos, but horn type cotyledonary embryos rarely germinated. The major cause of poor germination is the abnormal development of plumule apex meristem.

• Korean Journal of Pharmacognosy
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• v.30 no.1
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• pp.59-64
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• 1999

Supercritical fluid extraction (SFE) technique was applied to extract cytotoxic substances from five medicinal plants including Angelica gigas, Angelica acutiloba, Aralia cordata, Spirodela polyrhiza, Bupleurum falcatum, and Acanthopanax sessiliflorus. The cytotoxicities against P388, A549, and HL-60 cell lines were determined for the supercritical carbon dioxide extracts of five plant materials employed and were compared with those of the conventional organic solvent extracts such as n-hexane, $CHCl_{3}$, and MeOH to evaluate the SFE as an alternative method to conventional organic solvent extraction. In most cases, the SFE extracts of plant materials showed enhanced cytotoxicities when compared with those of other organic solvent extracts. In addition, the optimum temperature and pressure of SFE for extraction of the cytotoxic substances were largely affected by both the plant species and the cell lines tested. These results suggested that SFE could be an alternative to the conventional organic solvent method for the selective extraction of cytotoxic compounds from plants.

• Korean Journal of Plant Resources
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• v.8 no.3
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• pp.281-286
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• 1995

In order to get basic information for utilization of yong leabes of medicinal herbs as vegetable, 7 species leaves of medicinal herbs were analyzed. The moisture content in 6 species leaves were 79.74 to 85.97%. The raw protein content were about 2% except Angelica gigas (1.12%) and Peucedanum japonica (1.27%). The raw fat were 0.96 to 1.36%, the raw fiber were 1.8 to 3.9% and the raw ash were 1.58 to 2.79%. There content of medicinal herbs were similar to general vegetables. The K contents were the highest among the medical components in all samples. The total contents of amino acid in Aralza elata was 965mg%, Scutellaria baicalensis 930mg%, Aralia cordata 879mg%, Angelica acutiloba 851mg%, Agastache rugosa 747mg%, and Peucedanum japonica 615mg%, Aspartic acid and glutamic acid in amino acid were generally the highest but methionine and cystine the lowest.

• Journal of Sasang Constitutional Medicine
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• v.28 no.2
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• pp.103-109
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• 2016

Objectives This paper investigates the origin, the progressive changes and the constructive principles of Jeoryoungchajeonja-tang(猪苓車前子湯).Methods Jeoryoungchajeonja-tang and other related prescriptions were analyzed in terms of their pathological indications, based on previous literature including 『Sanghannon(傷寒論)』, 『Keumkuaeyoryak(金匱要略)』, 『Donguibogam(東醫寶鑑)』, 『Donguisusebowon‧Sasangchobongwon(東醫壽世保元‧四象草本卷)』, 『Donguisusebowon‧Gabogubon(東醫壽世保元‧甲午舊本)』, 『Donguisusebowon‧Sinchukbon(東醫壽世保元‧辛丑本)』Results and Conclusions 1) The origin of Jeoryoungchajeonja-tang is discovered in the prescriptions for Lee-su(利水), that is, Jeoryoung-tang(猪苓湯). 2) Ohryoung-san(五苓散) introduced in 『Donguisusebowon‧Sasangchobongwon(東醫壽世保元‧四象草本卷)』progressively transformed into Shihosaryoung-san(柴胡四苓散)(『Donguisusebowon‧Gabogubon(東醫壽世保元 ‧甲午舊本)』) and ultimately into Jeoryoungchajeonja-tang(『Donguisusebowon‧Sinchukbon(東醫壽世保元‧辛丑本)』), a prescription appropriate for usage in the Mangeum symptomatology(亡陰證). 3) The Jeoryoungchajeonja-tang is composed of 10 herbs. Of these, Notopterygium incisum Ting(羌活), Aralia cordata Thunb.(獨活), Schizonepeta tenuifolia Briq.(荊芥), Saposhnikovia divaricata Schischk.(防風) make the Pyoeum(表陰) drop, and Anemarrhena asphodeloides Bge.(知母), Gypsum fibrosum(石膏) scatter the uihwa(胃火), and Poria cocos Wolf.(茯苓), Alisma canaliculatum(澤瀉), Polyporus umbellatus Fr.(猪苓), Plantago asiatica L.(車前子) facilitate urination.

• Journal of Acupuncture Research
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• v.27 no.4
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• pp.39-53
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• 2010

목적 : 퇴행성 관절염은 염증성 사이토카인인 IL-$1\beta$에 의해 연골관절이 파괴되고 이로 인해 염증성 사이토카인이 더욱 증가하는 질환이다. 퇴행성 관절염을 치료하기 위해서는 연골 파괴를 가속화시키는 catabolic cytokines의 활성을 줄이고, 성장인자인 anabolic factor의 활성을 증가시는 연골 보호 작용이 있어야 한다. 본 연구에서는 독활 승마 처방(OAH19T)이 catabolic/anabolic 대사 조절에 어떤 영향을 미치는지와 그 신호 전달 기전에 대해 연구하였다. 또한 OAH19T를 구성하는 단미재 및 임상에서 사용되는 COX-2 inhibitor인 Celebrex(CEL)와 효능을 비교 실험하였다. 방법 : 배양된 세포에 IL-$1\beta$로 자극한 후 (1) glycosaminoglycan(GAG)의 분해 억제 정도, (2) OAH19T와 CEL에 대하여 MMP-1과 MMP-3의 유전자 발현 및 활성 억제, (3) Aggrecan 및 Aggrecanases의 유전자 발현 및 활성 억제, (4) OAH19T의 growth factor의 조절 능력, (5) MAPK pathway 등을 RT-PCR(reverse transcriptase-polymerase chain reaction), ELISA(Enzyme-linked immunosorbent assay), western blot, viability 측정을 통해 검증했다. 결과 : 사람 관절 세포에서 (1) 독활 승마 각각의 단미재, 임상에서 사용중인 셀레콕시브(CEL), 조인스보다 실험 약물(OAH19T)이 저농도에서 GAG 분해 억제 효과가 우수하였고, 부탄올로 분획한 OAH19B와는 동등한 효과를 보였다. (2) OAH19T는 IL-$1\beta$에 의하여 활성화된 MMP-1과 MMP-3의 발현을 모두 억제하였으나, CEL은 MMP-1의 발현은 억제하였으나 MMP-3의 발현은 억제하지 못하였다. (3) OAH19T는 IL-$1\beta$에 의하여 손상된 Aggrecan을 회복시켰으며 이는 활성화된 Aggrecanase-1과 Aggrecanase-2를 억제시킴으로써 나타난 결과이다. 그러나 CEL의 경우, 손상된 Aggrecan을 회복시키지 못하였다. (4) 배양된 세포는 IL-$1\beta$에 의하여 TGF-$\beta$II및 TGF-$\beta$ receptor II의 발현이 억제되었으나, OAH19T는 TGF-$\beta$II및 TGF-$\beta$ receptor II의 발현을 회복시켜 OAH19T가 anabolic한 조절능력이 있음을 시사한다. 그러나 CEL의 경우 growth factor에 대한 조절 능력이 없었다. (5) 대사 조절 작용에 대한 기전으로서 MAPK pathway에 대해서 연구한 결과 IL-$1\beta$에 의하여 유도된 pERK, pp38 kinase의 활성은 억제하였고, pJNK의 활성은 변하지 않았다. 또한 OAH19T는 연골 세포에 독성이 없었으며 IL-$1\beta$에 의해 유도된 세포 증식만을 억제시켰다. 이 결과로, OAH19T가 OA chondrocyte의 탈분화 및 세포 고사를 억제하여 연골보호 및 회복 효과가 있음을 알 수 있었다. 결론 : OAH19T는 이를 구성하는 단미재 및 CEL보다 연골보호 효과가 월등하였고, 이러한 연골보호 효과는 catabolic cytokines/growth factors의 균형으로 대사조절을 통해 연골세포의 탈분화 및 세포 고사를 억제하여 연골보호 및 회복 효과가 있음을 알 수 있었다.