• Archives of Pharmacal Research
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• 제16권2호
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• pp.104-108
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• 1993

From the root barks of Araila elata Seem.(Araliaceae) three known saponins together with oleanolic acid and $\beta$-sitosterol $3-O-\beta$-D-glucoside were isolated. The saponins were identified as oleanolic acid $28-O-\beta$-D-glucoside were isolated. The saponins were identified as oleanolic acid $28-O-\beta$-D-glucopyranosyl ester, oleanolic acid $3-O-\beta$-D-glucuronopyranoside and oleanolic acid $3-O-\alpha$-L-arabinofuranoysyl-(1$\rightarrow$4)-$\beta$-D-glucuronopyranoside(narcissiflo on the basis of chemical and spectra data. The latter two saponins were isolated as their dimethylesters as well as monomethylesters.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.415.2-415.2
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• 2002

We investigated the absorption enhancing effect of BuOH extract of the root of Aralia elata (BERAE) in Caco-2 cell monolayers and rats. At the concentration of both 0.04% and 0.08% (w/v). BERAE decreased the transepithelial electrical resistance (TEER) values and increased the permeability of intact chondroitin sulfate (CS) and its digestion products as hydrophilic macromolecules in a dose dependent manner. We also evaluated the cytotoxicity of BERAE for the determination of a proper concentration as an absorption enhancer. (omitted)

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2004년도 생명공학 실용화를 위한 비젼
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• pp.172-172
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• 2004

• 한국식생활문화학회지
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• 제5권2호
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• pp.235-241
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• 1990

두릅나무 Aralia elata순의 saponin을 분리하여 조사한 결과는 다음과 같다. 1. Saponin의 함량과 종류는 자연산이 온상산보다 많고, 가열에 의해서는 모두 감소하였으며, 잎이 자랄수록 그 함량은 증가하였다. 자연산 날것 식용적기(Nr2)의 총 saponin 종류는 37개였다. 2. 자연산의 saponin 패턴은 생육시기에 따라 변하였으며 날것 식용적기(Nr2)가 다른 것에 비해서 크게 달랐다. 그러나 가열 처리한 결과는 saponin 패턴이 변화되어 시기적 차가 줄었다. 온상산은 자연산에 비해 피크가 단순하고 시기별 차이는 적었지만 가열에 의한 패턴의 차도 적었다. 3. 자연산 날것 식용적기는 ‘b’ saponin이 특이하게 많았고 나머지에서는 ‘d’ saponin이 가장 많았다.

• 동의생리병리학회지
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• 제21권1호
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• pp.70-75
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• 2007

In the present study, we has been isolated the anti-cariogenic component, stigmasterol, from Aralia continentalis (A. continentalis) and identified by MS, $^1$H-NMR and $^{13}$C-NMR and also investigated the anti-cariogenic properties of stigmasterol. The methanol extract of ,A. continentalis showed concentration-dependent inhibitory activity against the growth and acid production of S. mutans. The MeOH extract was suspended in H$_2$O and sequentially partitioned with n-hexane, CHCl$_3$, EtOAc, and n-BuOH. The CHCl$_3$ fraction showed remarkable antibacterial activity against S. mutans. The anti-cariogenic compound, stigmasterol, has been isolated successively through the screening system and various chromatography methods. Anti-cariogenic properties of stigmasterol were also investigated. From this active chloroform subfraction, isolation and identification finally gave (24E)-stigmasta-5,22-dien-3${\beta}$-ol (stigmasterol) {[a]$_D\;^{25}$ -48.33$^{\circ}C$(C 0.28, CHCl$_3$)} by spectroscopic methods (MS, $^1$H-NMR and $^{13}$C-NMR) as an active principle. The compound, stigmasterol, showed significant growth, acid production, adhesion and water-insoluble glucan synthesis inhibitory effect against S. mutans. These results suggest that stigmasterol from ,A. continentalis may inhibit cariogenic properties of S. mutans and these properties may provide some scientific rationales that the local inhabitants used the extracts for treatment of dental diseases.

• 한국자원식물학회지
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• 제8권2호
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• pp.183-188
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• 1995

땅두릅 여룹철 연화재배(軟化栽培) 가능성(可能性)을 검토(檢討)하기 위하여 흑색(黑色) PE와 은색(銀色) 단섬유불직포(短纖維不織布)를 이용하여 5월 정식(定植)을 포함하여 6월, 7월, 8월 정식기(定植期)에 대하여 시험(試驗)한 결과(結果)는 다음과 같다. 1. 수확소요일수(收穫所要日數)는 5월 정식(定植)에서 20일(日)이고 7월, 8월 정식(定植)에서는 15일로 단축(短縮)되었으나 생육기간중(生育期間中)의 적산온도(積算溫度)는 $400^{\circ}C$ 범위이었다. 2. 7월 정식(定植)에서 땅두릅 연화수(軟化莖)의 수량(收量)은 고온(高溫)으로 인한 연부현상(軟腐現狀)으로 낮은 경향(傾向)이었다. 3. 각(各) 부위별(部位別) 경도(硬度)는 상위부(上位部)가 연(軟)하고 하위부(下位部)가 단단한 경향(傾向)이고, 저온저장(低溫貯藏) 7일후(日後)에도 신선도(新鮮度)는 유지(維持) 되었다. 4. 5월, 6월 정식재배시(定植栽培時) 18,000천원/ha 이상(以上)의 소득(所得)을 얻을 수 있었다.

• The Korean Journal of Physiology and Pharmacology
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• 제21권3호
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• pp.279-286
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• 2017

The root bark extract of Aralia taibaiensis is used traditionally for the treatment of diabetes mellitus in China. The total saponin extracted from Aralia Taibaiensis (sAT) has effective combined antihyperglycemic and hypolipidemic activities in experimental type 2 diabetic rats. However, the active compounds have not yet been fully investigated. In the present study, we examined effects of twelve triterpenoid saponins on AMP-activated protein kinase (AMPK) activation, and found that compound 28-O-${\beta}$-D-glucopyranosyl ester (AT12) significantly increased phosphorylation of AMPK and Acetyl-CoA carboxylase (ACC). AT12 effectively decreased blood glucose, triglyceride (TG), free fatty acid (FFA) and low density lipoprotein-cholesterol (LDL-C) levels in the rat model of type 2 diabetes mellitus (T2DM). The mechanism by which AT12 activated AMPK was subsequently investigated. Intracellular ATP level and oxygen consumption were significantly reduced by AT12 treatment. The findings suggested AT12 was a novel AMPK activator, and could be useful for the treatment of metabolic diseases.

• 한국약용작물학회지
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• 제13권4호
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• pp.182-185
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• 2005

사람들의 생명연장과 각종 만성질환, 악성종양 등의 증가에 따른 우수한 항생제의 계속적인 개발로 많은 생명을 구할 수 있게 되었지만 반면 항생제를 지나치게 사용하여 이에 많은 부작용이 생기고 내성균도 많아지면서 중증감염이 증가되어 여러 가지의 새로운 문제점에 직면하게 되었다. 안전성이 있는 새로운 항균물질의 개발 목적으로 예비 검색 결과 선택된 독활을 추출하여 컬럼 크로마토그래피를 반복 수행한 결과 2종의 항균 효과를 보여주는 화합물을 분리하였다 이들 화합물은 물리 화학적 성질 및 분광학적 분석을 통하여 화합물 1은 mp가 $166{\sim}167^{\circ}C$인 백색침상, $C_{20}H_{30}O_2$의 분자식을 갖는 (-)-pimara-8(14), 15-dien-19-oic acid 즉, continentalic acld으로 동정하였으며, 화합물 2는 mp가 $164{\sim}165^{\circ}C$인 백색침상, $C_{29}H_{48}O$의 분자식을 갖는 $(24E)-stigmasta-5,\;22-dien-3{\beta}-ol$, 즉 stigmasterol으로 동정하였으며 항균효과를 측정한 결과 두 화합물 모두 유의성 있는 결과를 나타내었으며, 이 중 화합물 1인 continentalic acid이 더욱 우수한 활성을 나타내었다. 특히 그람 양성균인 S. aureus에서 MIC가 $7.8\;{\mu}g/ml$, E. faecalis에서는 MIC가 $12.5\;{\mu}g/ml$에서 비교적 강한 억제 효과를 보였으며, 화합물 2에서는 S. aureus에서 MIC가 $7.8\;{\mu}g/ml$, S. epidemidis에서는 MIC가 $12.5\;{\mu}g/ml$에서 억제 효과를 보여 주었으나 그람 음성균에서 $100\;{\mu}g/ml$ 이상으로 항균활성이 측정되지 않았다. 따라서 화합물 1과 화합물 2는 그람 양성균에 대한 새로운 항균제로 개발에 유용한 화합물이 될 것으로 기대된다.

• Archives of Pharmacal Research
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• 제28권1호
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• pp.28-33
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• 2005

Seven diterpenes, four polyacetylenes, a lipid glycerol, and two sterols were isolated from the methylene chloride fraction of the root of Aralia cordata. Their chemical structures were determined as (-)-pimara-8(14), 15-dien-19-oic acid (2), pimaric acid (3), (-)-kaur-16-en-19-oic acid (4), 17-hydroxy-ent-kaur-15-en-19-oic acid (9), $7{\alpha}$-hydroxy-(-)-pimara-8(14), 15-dien-19-oic acid (10), $16\alpha$, 17 -dihydroxy-(-)-kauran-19-oic acid (11), 16-hydroxy-17-isovaleroyloxy-ent-kauran-19­oic acid (12), falcarindiol (5), dehydrofalcarindiol (6), dehydrofalcarindiol-8-acetate (7), falcarin­diol-8-acetate (8), alpha-mono palmitin (13), stigmasterol (1), and daucosterol (14) by the spectral evidences. These compounds were tested with COX-1 and COX-2 inhibition assays. This study found that compounds 2, 4, 5, 6, 7, 8, and 10 inhibited COX-1 dependent conversion of the exogenous arachidonic acid to $PGE_2$ in a dose-dependent manner with $IC_{50}$ values of $134.2{\mu}M$, $121.6{\mu}M$, $170{\mu}M$, $50.4{\mu}M$, $11.7{\mu}M$, $99.6{\mu}M$, and $69.6{\mu}M$, respectively. But, most of these compounds weakly inhibited COX-2 dependent $PGE_2$ generation. Among them, only compound 4 showed relatively significant inhibitory activity $(IC_{50}\;:\;127.6{\mu}M)$.

• 대한한의학방제학회지
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• 제21권1호
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• pp.161-176
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• 2013

Objectives : To clarify the anti-arthritic activity of Ostericum koreanum Maxim. (OS) plus Aralia continentalis Kitagawa (AC) in vivo. Methods : All mice were immunized with bovine type II collagen. After a second collagen immunization, mice were treated with OS plus AC once a day for 7 weeks. Oral administration of OS plus AC (200 or 50 mg/kg) significantly suppressed the progression of CIA, which extend is comparable to that of methotrexate (MTX, 0.3 mg/kg), a positive control. The severity of arthritis within the knee joints was evaluated by histological assessment of cartilage destruction and pannus formation. Results : Administration of OS plus AC significantly suppressed the progression of CIA and inhibited the production of TNF-${\alpha}$ and IL-6 in serum. The erosion of cartilage was dramatically reduced in mouse knees after treatment with OS plus AC. In conclusion, our results demonstrates that OS plus AC significantly suppressed the progression of CIA and that this action was characterized by the decreased production of IL-6, IFN-${\gamma}$ and collagen II specific antibody in serum, CD3+CD69+ T cells, MHC class II+/CD11c+ (in DLN), CD11b+Gr-1+ cells (in PBMC), CD11b +Gr-1+ cells, B220+/CD23+ (in paw joint). Conclusions : The the levels of IFN-${\gamma}$ in the culture supernatant of splenocytes stimulated with CD3/CD28 or collagen were dramatically decreased, while those of IL-4 was increased. In the serum of OS and AC-treated mice, the levels of IgM RA factor were decreased.