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Inhibitory Constituents against Cyclooxygenases from Aralia cordata Thunb  

Dang Nguyen Hai (College of Pharmacy, Chungnam National University)
Zhang XinFeng (College of Pharmacy, Chungnam National University)
Zheng MingShan (College of Pharmacy, Chungnam National University)
Son Kun Ho (Department of Food and Nutrition, Andong National University)
Chang Hyeun Wook (College of Pharmacy, Yeungnam University)
Kim Hyun Pyo (College of Pharmacy, Kangwon National University)
Bae KiHwan (College of Pharmacy, Chungnam National University)
Kang Sam Sik (Natural Products Research Institute and College of Pharmacy, Seoul National University)
Publication Information
Archives of Pharmacal Research / v.28, no.1, 2005 , pp. 28-33 More about this Journal
Abstract
Seven diterpenes, four polyacetylenes, a lipid glycerol, and two sterols were isolated from the methylene chloride fraction of the root of Aralia cordata. Their chemical structures were determined as (-)-pimara-8(14), 15-dien-19-oic acid (2), pimaric acid (3), (-)-kaur-16-en-19-oic acid (4), 17-hydroxy-ent-kaur-15-en-19-oic acid (9), $7{\alpha}$-hydroxy-(-)-pimara-8(14), 15-dien-19-oic acid (10), $16\alpha$, 17 -dihydroxy-(-)-kauran-19-oic acid (11), 16-hydroxy-17-isovaleroyloxy-ent-kauran-19­oic acid (12), falcarindiol (5), dehydrofalcarindiol (6), dehydrofalcarindiol-8-acetate (7), falcarin­diol-8-acetate (8), alpha-mono palmitin (13), stigmasterol (1), and daucosterol (14) by the spectral evidences. These compounds were tested with COX-1 and COX-2 inhibition assays. This study found that compounds 2, 4, 5, 6, 7, 8, and 10 inhibited COX-1 dependent conversion of the exogenous arachidonic acid to $PGE_2$ in a dose-dependent manner with $IC_{50}$ values of $134.2{\mu}M$, $121.6{\mu}M$, $170{\mu}M$, $50.4{\mu}M$, $11.7{\mu}M$, $99.6{\mu}M$, and $69.6{\mu}M$, respectively. But, most of these compounds weakly inhibited COX-2 dependent $PGE_2$ generation. Among them, only compound 4 showed relatively significant inhibitory activity $(IC_{50}\;:\;127.6{\mu}M)$.
Keywords
Aralia cordata; Diterpenoids; Polyacetylenes; Lipid glycerol; Steroids; Anti-inflammatory; COX-1; COX-2;
Citations & Related Records

Times Cited By Web Of Science : 22  (Related Records In Web of Science)
Times Cited By SCOPUS : 20
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