• 한국식품조리과학회지
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• 제23권3호통권99호
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• pp.294-301
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• 2007

본 연구에서는 피클의 특징인 아삭아삭함을 유지하면서 우리나라의 전통식 장아찌를 혼용한 땅두릅 피클을 제조하여 적합 가능성을 알아보고자 하였다. 땅두릅 피클을 제조하여 품질특성을 조사한 결과, pH는 저장기간이 경과함에 따라 비슷한 경향으로 감소하였으며 적정산도는 저장 초기부터 저장 5개월까지는 약간씩 증가하여 저장 6개월에는 급격히 증가하였다. 염도는 저장기간이 경과함에 따라 증가하였는데 저장초기에서 1개월 사이에 급격히 증가하다가 그 이후로는 완만하게 증가하였다. 총균수는 비슷한 경향으로 증가하였으며 유산균수의 변화는 저장 초기 모든 처리구에서 거의 측정이 되지 않았으며 저장 3개월째에 약 0.7${\sim}$1.5 log CFU/g이다가 그 이후에는 유산균수가 측정되지 않았다. 땅두릅 피클의 색의 변화는 저장기간별 외관적 품질 변화가 거의 없는 것으로 판명되었다. 경도는 간장피클과 소금피클에서 모두 CaCl$_2$ 용액 처리구 가 경도를 증가시켜 품질특성 항목에서 가장 좋은 것으로 나타났으며 관능검사에서는 CaCl$_2$ 용액 처리와 관계없이 모두 높게 평가되었다. 따라서 땅두릅 이용한 피클제조의 적합 가능성을 보여주었다.

• 식물조직배양학회지
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• 제28권5호
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• pp.273-276
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• 2001

두릅나무의 체세포배 유래 소식물체의 효과적인 순화 방법을 개발하고자 플라스틱 용기를 사용 4가지 인공배양토 및 MS 액체배지 등 7가지 수용액 처리를 통해 활착률 및 생장을 조사하였다. 상토는 perlite가 vermiculite보다 활착 및 생장에 양호하였고, 입자가 클수록 활착률 및 생장이 좋았다. 액체배지는 2차 증류수 처리가 가장 양호하여 96%의 활착률을 나타냈으며, 염류농도를 1/4 및 1/8로 낮춘 MS배지는 각각 92%의 활착률을 보인 반면 기본배지 및 1/2 MS배지는 활착률이 저조하였다. 한편 0.1% hyponex와 2% sucrose 처리는 84% 및 76%의 활착률을 각각 나타냈다. 결론적으로 두릅나무의 체세포배 유래 소식물체의 순화는 입자가 큰 perlite를 사용하고 증류수 처리 혹은 염류 농도를 낮춘 MS배지 처리로 효과적인 순화가 가능함을 보여주었다.

• Natural Product Sciences
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• 제15권1호
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• pp.50-54
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• 2009

A simple and reliable reversed phase HPLC method was developed to determine pharmacologically active compounds ent-continentalic acid, kaurenoic acid, and continentalic acid from the roots of Aralia cordata. Quantitative analysis of diterpenoids of A. cordata were performed by reverse phase C-18 column using a isocratic of acetonitrile : 0.1% trifluoroacetic acid(70:30) with UV detection at 205 nm. The correlation coefficients of each calibration curve of ent-continentalic acid, kaurenoic acid, and continentalic acid were 0.9987, 0.9983, and 0.9986, respectively. The detection limit of ent-continentalic acid, kaurenoic acid, and continentalic acid were $0.5{\sim}1.0\;{\mu}g/mL$, respectively. The contents of ent-continentalic acid (1), kaurenoic acid (2), and continentalic acid (3) of the roots of A. cordata collected from seventeen district markets in Korea were $0.09{\sim}0.75\;mg/g$, $1.09{\sim}5.43\;mg/g$, and $2.69{\sim}9.08\;mg/g$, respectively.

• 동의생리병리학회지
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• 제20권3호
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• pp.617-622
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• 2006

In the present study, we investigated the anticariogenic compounds from Aralia continertalis (A. continnentalis) has been isolated and identified by MS, $^1H-NMR$ and $^{13}C-NMR$. The MeOH extract was suspended in $H_2O$ and sequentially Partitioned with $CHCl_3$, EtOAc, and n-BuOH. The $CHCl_3$ fraction showed remarkable antibacterial activity against S. mutans. The antibacterial activity compounds against 5. mutans by MIC was isolated successively through the screening system and various chromatography methods. From this active chloroform subfraction, isolation and identification finally gave continentalic acid by spectroscopic methods (MS, $^1H-NMR$ and $^{13}C-NMR$) as an active principle. The compound, continentalic acid, showed significant growth, acid production, adhesion and water-insoluble glucan synthesis inhibitory effect against 5. mutars. These results suggest that continentalic acid from A. continentalis may inhibit cariogenic properties of S. mutans and these properties may provide some scientific rationales that the local inhabitants used the extracts for treatment of dental diseases.

• 한국약용작물학회지
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• 제22권2호
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• pp.105-112
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• 2014

The present study investigated an ethanol extract (SSB) of a mixture of three medicinal plants of Vitis amurensis, Aralia cordata, and Glycyrrhizae radix for possible neuroprotective effects on neurotoxicity induced by Amyloid ${\beta}$ protein ($A{\beta}$) (25-35) in cultured rat cortical neurons and antidementia activity in mice. Exposure of cultured cortical neurons to $15{\mu}M$ $A{\beta}$ (25-35) for 36 h induced neuronal apoptotic death. At $1-30{\mu}g/m{\ell}$, SSB inhibited neuronal death, elevation of intracellular calcium concentration ($[Ca^{2+}]_i$), and generation of reactive oxygen species (ROS) induced by $A{\beta}$ (25-35) in cultured cortical neurons. Memory impairment and increase of acetylcholinesterase activity induced by intracerebroventricular injection of mice with 16 nmol $A{\beta}$ (25-35) was inhibited by chronic treatment with SSB (25, 50 and 100 mg/kg, p.o., for 8 days). From these results, it is suggested that antidementia effect of SSB is due to its neuroprotective effect against $A{\beta}$ (25-35)-induced neurotoxicity and that SSB may have a therapeutic role in preventing the progression of Alzheimer's disease.

• 생명과학회지
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• 제18권3호
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• pp.357-362
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• 2008

독활 메탄올 추출물과 그 분획의 항혈전 및 항산화 효과를 확인하기 위하여 ADP를 이용하여 혈소판 응집억제효과를 탐색하고 DPPH 및 ABTS-radical 소거능을 측정하여 항산화능을 측정하였다. 그 결과 독활, 특히 EtOAc 분획은 ADP에 의해 유도된 혈소판응집과 트롬빈에 의한 혈소판 부착에 대하여 농도 의존적으로 저해효과를 나타내었으며, 높은 폴리페놀 함량과 가장 높은 라디칼 소거능을 통하여 독활의 항산화 효과를 확인하였다. 이상의 결과로 독활 EtOAc 분획의 높은 DPPH 및 ABTS radical 소거능이 항혈소판 효과에 영향을 미치는 것으로 사료된다.

• Journal of Microbiology and Biotechnology
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• 제16권9호
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• pp.1399-1404
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• 2006

The roots of Aralia continentalis (AC) have been used traditionally in Korean as a folk medicine for anti-inflammation and as an anti-rheumatic. In this study, we report that the ethyl acetate-soluble traction (ACE) of the methanolic extract of AC root inhibited the cell growth of various human cancer cell lines and induced apoptosis of HL-60, human promyelocytic leukemia cells. Its $IC_{50}$ values on growth inhibition were estimated to be $56.3{\mu}g/ml$ on HL-60, $87.2{\mu}g/ml$ on HepG2, $93.2{\mu}g/ml$ on HeLa, $135.5{\mu}g/ml$ on DU-145, and $135.8{\mu}g/ml$ on HT-29 cells. Interestingly, ACE showed no antiproliferative effect on normal lymphocyte cells used as control. Furthermore, nuclear DAPI staining revealed the typical nuclear features of apoptosis in the HL-60 cells exposed to $80{\mu}g/ml$ ACE, and a flow cytometric analysis of the HL-60 cells using propidium iodide showed that the apoptotic cell population increased gradually from 5% at 0 h to 16% at 12 h and 20% at 24 h after treated with $50{\mu}g/ml$ of ACE. TUNEL assay also revealed the apoptotic induction of the HL-60 cells treated with ACE. To obtain further information on the ACE-induced apoptosis, the expression level of certain apoptosis-associated proteins was examined using a Western blot analysis. Treatment of the HL-60 cells with ACE resulted in the activation of caspase-3, and subsequent proteolytic cleavage of poly(ADP-ribose) polymerase (PARP). The above results confirmed that the apoptosis in the HL-60 cells was induced by ACE, and that caspase-3-mediated PARP cleavage was involved in the process.

• 한국식품영양과학회지
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• 제41권4호
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• pp.571-575
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• 2012

참두릅과 개두릅가루의 단백질과 지방함량은 개두릅가루가 참두릅가루보다 많았으며, 회분과 식이섬유 함량은 참두릅가루보다 적었다. 참두릅과 개두릅가루는 높은 식이섬유 함량을 나타냈다. 두릅가루를 혼합하여 제조한 색도는 L값은 낮았으며, a값은 증가하였고 b값은 감소하였다. 조리면의 cutting force는 두릅가루를 첨가한 조리면이 대조군보다 낮게 나타났고 두릅가루의 첨가량이 많을수록 감소하는 것으로 나타났으며, 참두릅가루를 첨가한 조리면이 개두릅가루를 첨가한 조리면보다는 높은 것으로 나타났다. 국수의 기능성을 높이고 기호도를 향상시키기 위하여 참두릅가루와 개두릅가루를 3, 5, 7% 첨가한 조리면의 관능검사를 실험한 결과 5% 첨가한 국수가 가장 높은 관능적 평가를 받았다.

• 한국자원식물학회지
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• 제20권6호
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• pp.499-503
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• 2007

Antioxidant activity, total phenolics level and cytotoxicity of the methanol extracts from the young sprouts of 5 Korean woody salad plants were determined. Methanol extracts of Kalopanax pictus had the highest DPPH radical scavenging activity, with an $IC_{50}$ value of 23.5mg $100g^{-1}$, and followed by Valeriana fauriei(43.1mg $100g^{-1}$), and Morus alba(>100mg $100g^{-1}$). Total phenolic content showed the highest amount in methanol extracts from Kalopanax pictus(23.7mg $100g^{-1}$), and followed by Valeriana fauriei(22.7mg $100g^{-1}$), Aralia elata(16.8mg $100g^{-1}$) and Morus alba(14.2mg $100g^{-1}$). In a MTT assay, methanol extracts of Aralia elata with $IC_{50}$ values of 151.0 and $140.7{\mu}g\;mL^{-1}$ showed the most potent cytotoxicity on Calu-6 and MCF-7, respectively. On the other hand, methanol extracts of Kalopanax pictus($IC_{50}=96.5{\mu}g\;mL^{-1}$) showed the highest activity against HCT-116, and followed by those of Aralia elata($123.3{\mu}g\;mL^{-1}$), and Actinidia arguta($162.0{\mu}g\;mL^{-1}$). Total phenolic content of the tested plant extracts was correlated with the DPPH radical scavenging activity, suggesting the phenolic compounds may contribute to the antioxidant properties of Korean salad plants.

• 동의생리병리학회지
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• 제23권6호
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• pp.1314-1319
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• 2009

In previous study, we have shown that continentalic acid (CA) isolated from Aralia continentalis induced the growth inhibition and apoptosis in HepG2 cells. In this study, we examine the effects of CA from A. continentalis on growth inhibition and apoptosis induction in human leukemia HL-60 and mouse fibroblast NIH 3T3 cell lines. The results demonstrated that CA decreased cell growth of leukemia HL-60 cells but not human HaCaT keratinocytes, assessed with the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and LDH (lactate dehydrogenase) assay. Flow cytometric analysis of mouse fibroblast cell lines exposed to CA showed that apoptotic cells increased in a time- and dose-dependent manner. Treatment with CA decreased the number of normal cells and increased the number of early apoptotic and late apoptotic cells in a dose-dependent manner. The induction of apoptosis in mouse cell lines by CA was mediated through the activation of caspase-3, Bak, and Bax and the down-regulation of Bcl-2. Our results suggest that CA efficiently induces apoptosis in human leukemia cells.