• 복식문화연구
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• 제12권3호
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• pp.407-414
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• 2004

A natural dye aqueous walnut shell extract was obtained by extraction of walnut shell using water at various conditions in this study. Silk fabrics were dyed at various dyeing and mordanting conditions using walnut shell extract and various mordants (Al, Cu, Fe ions). Studies have been made on the effects of dyeing and mordanting conditions on the dyeing properties and fastness (light, water and dry cleaning fastness) of dyed silk Fabric. The results obtained in this study were as follows;. The dye content in the walnut shell extract increased with increasing extraction temperature to 90℃ and extraction time to 120min, and thereafter the dye content decreased a little. The dry cleaning and water fastness of non-mordanted silk fabrics were better than those of mordanted silk fabrics.

• Advances in Traditional Medicine
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• 제2권2호
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• pp.101-105
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• 2002

A battery of neuropharmacological experiments showed the hot water extract of Ocimum sanctum Linn. (Labiateae) had a depressant effect on the central nervous system (CNS), but the aqueous extract showed no effect on it. The hot water extract reduced the spontaneous locomotor activity, exploratory head dipping, propulsive locomotion and exploratory ambulation as well as prolonged the pentobarbital induced sleeping time. The depressant effect starts from 60 minutes after the drug administration and continued to 180 minutes. The drug may exert central depressant effect by interfering with the function of the cortex.

• 한국자원식물학회지
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• 제5권1호
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• pp.49-56
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• 1992

In the preliminary antitumor screening tests of Crude Drugs and collected plants, the methanol ic extract of Forsythia Fruits in Korean marcket showed signif icant cytotoxic activity against Chinese hamster V-79 cells, but that in Japanese market did not. The former was identifid as Forsythia viridissima Lindley and latter as F.suspensa Vahl on the basis of the morphological observation. When an aqueous solution of the extract prepared from the fruits of F. viridissima (Oleaceae) was partitioned successively with n-hexane, methylene chloride, n-butanol, the cytoyoxic activitywas concetrated in the methylene chloride extract. Fractionation of the extract was made with the guidance of bioassay against V-79 cells to give cytotoxic lignans, matariresiol (1) and arctigenin (2). Their ICso values of compounds 1 and 2 were respectively $7.8{\;}\mu\textrm{g}/ml$ 1 and $1.65{\;}\mu\textrm{g}/ml$. Also, their structures were confirmed by comparison of physical and spectral data in the literature.

• Fisheries and Aquatic Sciences
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• 제13권1호
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• pp.12-17
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• 2010

This study was conducted to evaluate the potential of a microbial fermentation procedure to improve the functional qualities of seaweeds. Aspergillus oryzae, which has been used in traditional Korean fermented foods, was inoculated and cultivated in an aqueous extract of sea tangle (Laminaria japonica). Fermentation of the sea tangle extract by A. oryzae for 4 days resulted in a 3-fold increase in $\gamma$-aminobutyric acid (GABA) content. GABA is known to be a bioactive compound. Fungal fermentation of the extract also enhanced its antioxidant activity and increased its total content of phenolic compounds. It was assumed that these changes stemmed from the biodegradation of active compounds of the sea tangle packaged within its rigid structural matrix or occurred as result of fungal fermentation. These results suggested that the application of microbial fermentation to the processing of seaweeds will help in the development of processed foods to meet consumer demands.

• Advances in Traditional Medicine
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• 제4권2호
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• pp.95-99
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• 2004

The ethanolic extract of some medicinal plants having anti-asthmatic activity such as Solanum xanthocarpum, Curcuma longa, Glycyrrhiza glabra, Piper longum, A. vasica, A. lebbeck, and Tinospora cordifolia was evaluated for antihistaminic and anti-cataleptic activity. The aqueous solution of ethanolic extract of S. xanthocarpum and G. glabra potentiated histamine-induced tracheal chain contractions. Whereas, C. longa, P. longum, and T. cordifolia, and A. lebbeck were without any significant effect on histamine. Only A. vasica inhibited histamine-induced tracheal chain contraction. G. glabra per se produced contraction of the tracheal chain, which was blocked by pretreatment with atropine. Single dose of S. xanthocarpum potentiated clonidine-induced catalepsy but on repeated doses (once in a day for 3 days) inhibited catalepsy. Pretreatment with ethanolic extract of C. longa, P. longum, T. cordifolia inhibited catalepsy whereas G. glabra and A. lebbeck significantly potentiated clonidine-induced catalepsy. None of the extracts inhibited haloperidol-induced catalepsy. Thus the extracts having antihistaminic activity or mast cell stabilizing activity inhibited clonidineinduced catalepsy.

• The Korean Journal of Ecology
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• 제20권2호
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• pp.103-109
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• 1997

Eleven phenolic compounds including caffeic acid were identified through analyzing the aqueous extracts of Pinus rigida by HPLC. Among them, protocatechuic acid was the maximum amount of 6.84 ppm. Seed germination of Cassia mimosoides var. nomame was significantly stimulated by the extract of P. rigida leaves in the proportion ot concentration. However, root growth was elevalted at a threshold concentration below 25%, but it was inhibited at high concentrations. In 50% extract of P. rigida, upward root tip of C. mimosoides var. nomame showed negageotropism which the root end showed necrosis. New isozyme bands were induced indicating concentration activity of peroxidase from the extract of C. mimosoides var. nomame, especially in the cathodic region. Although it reduced the mumber of isozyme bands of esterase, esterase activities were stimulated in the anodic region of C. mimosoides var. nomame. The activity of amylase was not remarkably different between control and treatment.

• 한국식품영양과학회지
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• 제38권12호
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• pp.1640-1648
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• 2009

본 연구에서는 조릿대 잎의 물과 70% 에탄올 조추출물 2종 및 70% 에탄올 조추출물의 분획 5종에 함유된 총 폴리페놀과 총 플라보노이드 함량을 정량하고, 이들 조추출물과 분획의 항산화 활성을 검증하고자 전자공여능, SOD 유사활성, 환원력 및 지질과산화물 생성억제능을 측정하였다. 조추출물 2종의 수율은 물과 70% 에탄올 각각 8.5%와 11.4%이었다. 분획 5종의 수율은 5.1-0.6%이었으며, aqueous, n-butanol, n-hexane, chloroform, ethyl acetate 분획 순으로 높았다. 총 폴리페놀 함량은 물과 70% 에탄올 추출물은 두 조추출물 간에 유의한 차이가 없었으나 총 플라보노이드 함량은 70% 에탄올 추출물이 물 추출물보다 유의하게 많았다. 분획 5종의 총 폴리페놀 함량은 chloroform, ethyl acetate, n-butanol, aqueous, n-hexane 분획 순으로 많았고, chloroform 분획은 타 분획에 비해 유의성 있게 많았다. 각 분획별 총 플라보노이드 함량은 ethyl acetate, chloroform, n-hexane, n-butanol, aqueous 분획 순으로 함량이 높았으며, chloroform과 ethyl acetate 분획은 타 분획에 비해 유의적으로 많았다. 물과 70% 에탄올 조추출물은 모두 BHT에 비해, 유의성 있게 높거나 또는 같은 수준의 지질과산화물 생성억제능을 보였으며, 동일한 수준의 SOD 유사활성을 나타내었다. 다만, 전자공여능과 환원력은 BHT에 비해 낮았다. 물과 70% 에탄올 조추출물 간에 이들 항산화 활성은 다르지 않았다. 다만, 전자공여능만 일부 농도에서 70% 에탄올 조추출물이 물 조추출물에 비해 유의하게 높았다. 분획 5종은 모두 BHT에 비해 유의성 있게 높거나 같은 수준의 지질과산화물 생성억제능을 보였다. Chloroform과 ethyl acetate 및 n-butanol의 세 분획은 전자공여능과 SOD 유사활성도 BHT에 비해 유의하게 높았다. 다만, 환원력은 분획 5종 모두 BHT보다 낮았다. 조추출물 2종과 분획 5종의 전자공여능이나 SOD 유사활성, 환원력 및 지질과산화물 생성억제능 모두 본 연구에서 실험한 농도 범위에서, 농도 의존적으로 증가했거나 또는 증가하는 추세를 보였다. 이러한 연구결과에 기초해 조릿대 잎의 물 또는 70% 에탄올의 조추출물이나 70% 에탄올의 조추출물의 chloroform과 ethyl acetate 및 n-butanol 분획은 천연 항산화제로서 특히, 지질의 과산화를 방지하는 용도로서의 활용 가치가 있다고 결론지을 수 있다. 또한 건강기능성 소재로서의 식품산업 분야에 특히, 지질과산화물 생성억제능과 SOD 유사활성이 BHT보다 우수했고, 전자공여능을 보인 점은 이들 추출물이나 분획이 생체 내에서 활성산소종을 제거하고 생체막의 구성성분의 과산화를 방지하는 등 건강기능성 작용을 수행할 것이란 점을 시사한다.

• Journal of Applied Biological Chemistry
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• 제49권4호
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• pp.165-170
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• 2006

The antifungal activity of crude methanolic extract and fractions from Yucca smalliana Fern. leaves, roots and flowers were investigated in vitro against a panel of plant pathogenic fungi. The minimal inhibitory concentration(MIC) was determined by an agar dilution method. Preliminary liquid culture and agar plate assays showed that the growth of Fu sarium oxysporum, Phytophthora capsici, Rhizoctonia solani and Botrytis cinerea were inhibited by Y. smalliana extracts. The extracts from flowers and leaves showed antifungal activity of 64.0% and 34.0% against F. oxysporum, 66.0% and 62.0% against P. capsici, and 27.0% and 41.0% against B. cinerea, respectively. The methanolic extract from Y. smallina leaves in distilled water was fractionated using solvents of increasing polarity: hexane, ethyl acetate and butanol. These fractions had a broad spectrum of antifungal activity, found to reside entirely in the butanol and aqueous fraction. The aqueous fraction showed inhibition rate of 60.0, 67.8, 84.6 and 58.3% against F. oxysporum, R. solani, C. gloeosporioides, and B. cinerea, respectively, and the butganol fracgtion showed 36.0, 46.0, 66.1 and 58.3%, respectively. Phenolics(e.g. flavonoids, steroids and terpenoids) were observed in the thin layer profile of the different fractions. Leave extract showed a prominent antioxidant activity totally scavenging the free radical of DPPH at a concentration of 1 mg/ml.

• 한국토양비료학회지
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• 제25권3호
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• pp.263-269
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• 1992

잡초추출물에 의한 작물의 생장반응 및 토양미생물에 미치는 영향과 추출물의 phenolic Compounds 분리에 대한 시험결과를 요약하면 다음과 같다. 1. 잡초추출물에 의하면 대두(大豆), 육도(陸稻), 토마토 공(共)히 발아 및 생장에 영향을 받았는데 특(特)히 쇠비름추출물(5% 추출액)에서 대두(大豆)의 생장이 8~12%의 억제를 나타내었다. 2. 작물의 광합성작용에 대한 잡초추출물의 억제효과는 쇠비름(5% 추출액)이 대두(大豆)의 광합성작용(光合成作用)에 60%의 억제효과를 보였다. 3. 토양중 처리된 phenol화합물은 농도가 높을수록 방선균과 세균의 수(數)는 감소하는 경향을 보였으나 사상균은 증가(增加)하는 추세를 보였다. 4. HPLC에 의하여 분리된 phenol물질은 9종(種)이었으며, p-hydroxybenzoic등 2종(種)이 모든 잡초추출물에 검출되었다. 5. Fusarium Spp.에 대한 Vanillic acid의 억제는 농도가 높을수록 효과가 크게 나타났다.

• Nutrition Research and Practice
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• 제15권5호
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• pp.579-590
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• 2021

BACKGROUND/OBJECTIVES: Petasites japonicus Maxim (P. japonicus) has been used as an edible and medicinal plant and contains many bioactive compounds. The purpose of this study is to investigate the effect of P. japonicus on osteogenesis. MATERIALS/METHODS: The leaves and stems of P. japonicus were separated and extracted with hot water or ethanol, respectively. The total phenolic compound and total polyphenol contents of each extract were measured, and alkaline phosphatase (ALP) activity of each extract was evaluated to determine their effect on bone metabolism. To investigate the effect on osteoblast differentiation of the aqueous extract of P. japonicus leaves (AL), which produced the highest ALP activity among the tested extracts, collagen content was measured using the Sirius Red staining method, mineralization using the Alizarin Red S staining method, and osteocalcin production through enzyme-linked immunosorbent assay analysis. Also, real-time reverse transcription polymerase chain reaction was performed to investigate the mRNA expression levels of Runt-related transcriptional factor 2 (Runx2) and Osterix. RESULTS: Among the 4 P. japonicus extracts, AL had the highest values in all of the following measures: total phenolic compounds, total polyphenols, and ALP activity, which is a major biomarker of osteoblast differentiation. The AL-treated MC3T3-E1 cells showed significant increases in induced osteoblast differentiation, collagen synthesis, mineralization, and osteocalcin production. In addition, mRNA expressions of Runx2 and Osterix, transcription factors that regulate osteoblast differentiation, were significantly increased. CONCLUSIONS: These results suggest that AL can regulate osteoblasts differentiation, at least in part through Runx2 and Osterix. Therefore, it is highly likely that P. japonicus will be useful as an alternate therapeutic for the prevention and treatment of osteoporosis.