Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Antifungal and Antioxidative Activities of Yucca smallina Fern

  • Jin, Yu-Lan (Department of Plant Protection, Laiyang Agricultural College) ;
  • Jung, Woo-Jin (Glucosamine Saccharide Materials-National Research Laboratory(GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Kuk, Ju-Hee (Glucosamine Saccharide Materials-National Research Laboratory(GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Kim, Jung-Bong (National Institute of Agriculture Biotechnology(NIAB)) ;
  • Kim, Kil-Yong (Glucosamine Saccharide Materials-National Research Laboratory(GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Park, Ro-Dong (Glucosamine Saccharide Materials-National Research Laboratory(GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University)
  • Published : 2006.12.31
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Abstract

The antifungal activity of crude methanolic extract and fractions from Yucca smalliana Fern. leaves, roots and flowers were investigated in vitro against a panel of plant pathogenic fungi. The minimal inhibitory concentration(MIC) was determined by an agar dilution method. Preliminary liquid culture and agar plate assays showed that the growth of Fu sarium oxysporum, Phytophthora capsici, Rhizoctonia solani and Botrytis cinerea were inhibited by Y. smalliana extracts. The extracts from flowers and leaves showed antifungal activity of 64.0% and 34.0% against F. oxysporum, 66.0% and 62.0% against P. capsici, and 27.0% and 41.0% against B. cinerea, respectively. The methanolic extract from Y. smallina leaves in distilled water was fractionated using solvents of increasing polarity: hexane, ethyl acetate and butanol. These fractions had a broad spectrum of antifungal activity, found to reside entirely in the butanol and aqueous fraction. The aqueous fraction showed inhibition rate of 60.0, 67.8, 84.6 and 58.3% against F. oxysporum, R. solani, C. gloeosporioides, and B. cinerea, respectively, and the butganol fracgtion showed 36.0, 46.0, 66.1 and 58.3%, respectively. Phenolics(e.g. flavonoids, steroids and terpenoids) were observed in the thin layer profile of the different fractions. Leave extract showed a prominent antioxidant activity totally scavenging the free radical of DPPH at a concentration of 1 mg/ml.

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References

  1. Cheeke, P. R., Piacente, S. and Oleszek, W. (2006) Antiinflammatory and anti-arthritic effects of Yucca schidigera: A review. J. Inflamm. 3, 1-7 https://doi.org/10.1186/1476-9255-3-1
  2. Torres, P., Avila, J. G., Vivar, A. R., García, A. M., Marín, J. C., Aranda, E. and Céspedes, C. L. (2003) Antioxidant and insect growth regulatory activities of stilbenes and extracts from Yucca periculosa. Phytochemistry. 64, 463-473 https://doi.org/10.1016/S0031-9422(03)00348-0
  3. Asaka, O. and Shoda, M. (1996) Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14. Appl. Environ. Microbiol. 62, 4081-4085
  4. Shim, J. O. and Lee, M. W. (1994) Growth rate and colony morphology of progenies of zoospores and oospores of Phytophthora cactorum causing phytophthora rots in apple trees. Kor. J. Mycol. 22, 281-285
  5. Bors, W., Saran, M. and Eltsner, E. F. (1992) Modern Methods of Plant Analysis. New Series, Academic Press, N.Y
  6. Cuendet, M., Hostettmann, K., Potterat, O. and Dyatmiko, W. (1997) Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helv. Chim. Acta 80, 1144-1152 https://doi.org/10.1002/hlca.19970800411
  7. Killeen, G. F., Madigan, C. A., Connolly, C. R., Walsh, G. A., Clark, C., Hynes, M. J., Timmins, B. F., James, P., Headon, D. R. and Power, R. F. (1998) Antimicrobial saponins of Yucca schidigera and the implications of their in vitro properties for their in vivo impact. J. Agr. Food Chem. 46, 3178-3186 https://doi.org/10.1021/jf970928j
  8. Uematsu, Y., Hirata, K., Suzuki, K., Iida, K. and Kamata, K. (2004) Investigation of spectrophotometrically determined substances in yucca extract by GC/MS, TLC and on-column injection GC. Shokuhin Eiseigaku Zasshi. 3, 141-145
  9. Piacente, S., Bifulco, G., Pizza, C., Stochmal, A. and Oleszek, W. (2002) A novel phenolic spiro derivative, Yuccaone A, from Yucca schidigera bark. Tetrahedron Lett. 43, 9133-9136 https://doi.org/10.1016/S0040-4039(02)02244-X
  10. Grayer, R. J. and Harbone, J. B. (1994) A survey of antifungal compounds from high plants. Phytochemistry. 37, 19-42 https://doi.org/10.1016/0031-9422(94)85005-4
  11. Rimando, A., Cuendet, M., Desmarchelier, C., Metha, R. G., Pezzuto, J. M. and Duke, S. O. (2002) Cancer chemopreventive and antioxidant activities of pterostilbene, a naturally occurring analogue of resveratrol. J. Agric. Food Chem. 50, 3453-3457 https://doi.org/10.1021/jf0116855
  12. Burns, J., Yokota, T., Ashihara, H., Lean, M. E. J. and Crozier, A. (2002) Plant foods and herbal sources of resveratrol. J. Agric. Food Chem. 50, 3337-3340 https://doi.org/10.1021/jf0112973
  13. Doh, E. S. (1999) Antifungal of Coptis japonica root-stem extract and identification of antifungal substances. Korean J. Plant. Res. 12, 260-268
  14. Yu, S.Y., Kim, J. C., Kim, Y. S., Chae, G. J., Kim, S. G., Kim, H. T., Kim, J. S., Lee, S. U. and Jo, G. Y. (2001) Antifungal activities of coumarins isolated from Angelica gigas and Angelica dahurica against plant pathogenic fungal. Kor. J. Pesticide Sci. 5, 26-35