• Biomolecules & Therapeutics
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• 제13권4호
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• pp.232-239
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• 2005

An aqueous extract of oriental herbal composition named Onchengyeum and curcumin, an antioxidant isolated from turmeric (Curcuma Zonga L.) reduced hepatotoxicity induced by carbon tetrachloride ($CCI_4$). Improved liver function was observed by measuring the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), blood urea nitrogen (BUN), creatinine (CRE), total cholesterol (T-CHO), triglyceride (TG), low density lipoprotein cholesterol (LDL-CHO), high density lipoprotein cholesterol (HDL-CHO), total protein (TP), albumin (ALB) and total bilirubin (BIL) in serum. Hepatic parameters monitored were levels of cholesterol (CHO), triglyceride (TG), and malondialdehyde (MDA) and activities of cytochrome P450 (CYP), NADPH-CYP reductase, superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione S-transferase (GST), glutathione reductase (GR), and glutathione peroxidase (GPx). The histopathological examination showed that the treatment of Onchengyeum and curcumin relieved the ballooning degeneration of hepatocytes which had been generated by $CCI_4$. The results suggested that hepatoprotective effects of Onchengyeum and curcumin possibly are due to their promising antioxidative activity.

• 생약학회지
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• 제27권4호
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• pp.378-382
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• 1996

We previously reported the morphological, physiochemical characteristics of Alismae Rhizoma. In this study, we examined the antimicrobial and antifungal activities of 85% ethanol and water extracts of Alismae Rhizoma using disc method. Ethanol extracts of Korean Alismae Rhizoma showed antimicrobial activity on B. subtilis and S. aureus on the concentration dependent manner, whereas, Chinese Alismae Rhizoma revealed antimicrobioal activity on E. coli and N. gonorrhoeae. The water solution(500 mg/ml) of each lyophilized powder of aqueous extracts didn't show any antimicrobial activity on B. subtilis, S. aureus and E. coli, on the contrary, they stimulated cell growth slightly. Ethanol extracts of Korean samples showed antifungal activity on A. niger, but not on C. albicans. Neither A. niger nor C. albicans were affected by Chineses samples.

• Advances in Traditional Medicine
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• 제9권1호
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• pp.74-82
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• 2009

Pharmacological investigations were carried out with aqueous methanolic extract (AME) of Swietenia mahagoni (L.) Jacq. (Meliaceae) leaves. Acute toxicity studies revealed that the $LD_{50}$ dose of AME was 600 mg/kg, i.p. AME was found to possess significant anti-inflammatory activity in acute, sub-chronic and chronic models of inflammation. AME selectively inhibited cyclooxygenase (COX)-2 activity, which is involved in arachidonic acid metabolism and biosynthesis of prostaglandins under inflammatory conditions. Treatment with AME significantly enhanced total peritoneal cell count and the number of macrophages in normal mice, which revealed that AME may also alter the immune response along with its anti-inflammatory effect. The saponins or the alkaloids present in AME may be responsible for the anti-inflammatory activity.

• 대한한의학회지
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• 제28권4호
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• pp.124-135
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• 2007

Backgrounds : Scutellaria baicalensis has been used as a medicinal plant to treat various disease conditions accompanying inflammatory response and oxidative stress. Objectives : The aim of this study is to evaluate the effects of Scutellaria baicalensis against inflammatory, pain and edema Methods : In vitro, the effects of Scutellaria baicalensis against lipopolysaccharide-induced inflammation were investigated in mouse BV2 microglial cells. In vivo, the effects of Scutellaria baicalensis on acetic acid-induced writhing response, carrageenan-induced edema and the plantar test (nociceptive thermal stimulation) were investigated using rats and mice. Results : The present results showed that pre-treatment with the aqueous extract of Scutellaria baicalensis suppressed the lipopolysaccharide-stimulated cyclooxygenase-2 expressions in mouse BV2 microglial cells. The aqueous extract of Scutellaria baicalensis inhibited acetic acid-induced abdominal pain in mice and also reduced thermal pain in rats. However, no significant inhibition on carrageenan-induced edema in rats. Conclusions : The present study showed that Scutellaria baicalensis possesses anti-inflammatory and analgesic effects.

• Toxicological Research
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• 제5권1호
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• pp.1-7
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• 1989

Toxic effects of a mushroom, Amanita volvata, were studied in respect to biochemical and histological changes induced in the liver and kidneys of mice. The changes in biochemical parameters characteristically appeared 12 hrs after oral administration of an aqueous extract of the mushroom. The hepatic glycogen decreased markedly to 17% of the control level and a concemitant decrease in blood glucose was also observed. The activity of serum glutamic oxaloacetic transaminase (SGOT) was elevated by 2.5-fold and the level of blood urea nitrogen (BUN) increased by 3-fold, respectively, 12 hrs after administration of the mushroom, their levels being maintained up to 24 hrs.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.113.3-114
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• 2003

The increased deposition of extracellular matrix by hepatic stellate cells following liver injury in a process known as activation is considered a key mechanism for increased collagen content of liver during the development of liver fibrosis. In this study, we investigated the protective effects of an aqueous extract from the roots of Platycodon grandiflorum A. DC (Campanulaceae), Changkil (CK), on hepatic fibrosis in hepatic stellate cells. We report that CK reduces the accumulation of collagen in acetaldehyde-induced hepatic stellate cells. (omitted)

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.288.1-288.1
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• 2002

Herbal medicines are increasingly being utilized to treat a wide variety of disease processes. The aim of this study was to evaluate the ability of aqueous extract from the roots of Platycodon grandiflorum A. DC (Campanulaceae). Changkil (CK). to affect cellular response in primary cultures of rat hepatocytes to t-butyl hydroperoxide (t-BHP) induced oxidative stress and hepatotoxicity. CK-treated cells showed an increased resistance to oxidative challenge. as revealed by a higher percent of survival capacity in respect to control cells. CK added prior or simultaneously with I-BHP reduced enganced lipid peroxidation measured as production of malondialdehyde and enhnaced intracellular reduced glutathinoe depletion by t-BHP. Furhtermore. CK protected from the t-BHP-induced intracellular generation of reactive oxygen species assessde by montioting dichlorodihydrofluorescein fluorescence. it can be concluded that CK exerts an antioxidant action insice the cell. responsible for the abserved modulation of the cellular response to oxidative challenge. and CK have a marked anitioxdative and hepatoprotective potency.

• 생명과학회지
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• 제22권7호
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• pp.889-896
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• 2012

멜라닌 생합성은 tyrosinase의 효소적 산화반응에 의해 일어난다. 톳(Hizikia fusiformis)의 미백효과를 검증하기 위하여 메탄올, 헥산, 부탄올 및 물을 용매로 이용한 분획과정을 통해 톳 에탄올 추출물로부터 분획물을 제조하였다. 에탄올 추출물과 분획물들을 대상으로 B16F10 흑색종 세포에서 tyrosinase 활성 저해효과 및 멜라닌 생합성 억제효과를 평가하였다. 생쥐의 에탄올 추출물 및 수층 분획물은 미백효과를 나타냈지만, 세포독성은 나타내지 않았다. 에탄올 추출물은 시료들 중에서 가장 높은 미백활성을 보였다. 에탄올 추출물은 $100{\mu}g/ml$의 농도에서 arbutin $10{\mu}g/ml$의 활성보다 높은 저해활성을 보였으나, kojic acid $10{\mu}g/ml$의 활성보다는 낮은 저해활성을 보였다. 또한, 메탄올, 헥산, 부탄올 및 수층 분획물은 $100{\mu}g/ml$의 농도에서 arbutin $10{\mu}g/ml$의 활성과 유사한 저해활성을 보였다. 항산화 활성은 L-ascorbic acid를 양성 대조군으로 하여 수치를 비교하여 나타냈다. 에탄올 추출물 및 수층 분획물의 DPPH 라디칼 소거활성은 다른 시료들보다 비교적 높게 나타났다. 또한, 에탄올 추출물및 수층 분획물은 $500{\mu}g/ml$의 농도에서 LPS에 의해 유도된 iNOS 발현을 각각 82와 80% 감소시켰다. 이러한 결과들로 톳의 에탄올 추출물과 수층 분획물은 미백효과 및 피부 보호효과를 가지는 화장품 소재로 이용 가능할 것으로 기대된다.

• 한국유기농업학회지
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• 제23권2호
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• pp.311-322
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• 2015

본 연구는 개여뀌를 이용하여 친환경 농자재로 개발하기 위한 기초자료를 제공하기 위해 수용성 추출액의 농도에 따른 재배작물인 감자생육에 미치는 알레로패시 효과를 조사하였다. 감자 시험구 처리는 개여뀌추출물 무처리, 25%, 50%, 75%, 100%로 하였으며, 개여뀌추출물의 처리는 정식 1일 후부터 5일 간격으로 처리구에 따라 관주하였다. 개여뀌의 수용성 추출액농도가 증가함에 따라 대조구에 비하여 맹아가 완료되는 시기가 1일이 빨랐고, 경수는 0.3-0.4/1주 개가 많았다. 경장은 생육초기에는 개여뀌추출물 처리구가 무처리구에 비해 컸으나, 생육 중반 이후에는 차이를 보이지 않았으며, 경직경 및 지상부 생체중은 개여뀌 처리구가 무처리구에 비해 양호한 경향을 보였으나 통계적 유의성은 없었다. 감자의 총수량/$m^2$은 개여뀌를 처리한 100%구에서 $13.8kg/m^2$로 가장 많았으며 처리간에 유의차가 인정되었다. 감자의 크기별 개수는 4 g 이하, 5-30 g 미만의 괴경 수량은 각 처리간에 일정한 경향을 보이지 않았으나 31 g 이상의 괴경 수량은 무처리구에 비해 개여뀌 수용성 추출물 처리구가 현저히 높았다. 이들 결과를 종합하면 개여뀌의 allelopathy 효과는 감자의 생육과 수량형질이 촉진되어 친환경 비료로써 활용 가능성을 가지고 있는 것으로 판단된다.

• 한방재활의학과학회지
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• 제24권2호
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• pp.31-40
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• 2014

Objectives Trachelospermi caulis, known as Nak-Suk-Deung in Korea, is the dried leafy stem of Trachelospermum asiaticum var. intermedium Nakai, and climbing stems and branches of Trachelospermum sdisyivum var, intermedium nakai or Apocyanaceae. Trachelospermi caulis has antipyretic and analgesic activity. It has traditionally been used as a folk remedy in Korea for the treatment of various infla mMatory diseases, including rheumatoid arthritis. The purpose of this study was to evaluate the Effects of Trachelospermum caulis extract on SNP-induced infla mMatory responses in rabbit HIG-82 synovial membrane cells. Methods Anti-infla mMatory effects of the extract of Trachelospermum caulis were investigated using rabbit HIG-82 synovial membrane cells. For this study, 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, western blot analysis, PGE2 i mMunoassay, and NO detection were conducted. Results The aqueous extract of Trachelospermum caulis exerted cytotoxicity and suppressed PGE2 synthesis and NO production in rabbit HIG-82 synovial membrane cells. The aqueous extract of Trachelospermum caulis also inhibited the SNP-induced expressions of COX-2, iNOS, and TNF-$\alpha$ in rabbit HIG-82 synovial membrane cells. Conclusions These results showed that the extract of Trachelospermum caulis exerts the anti-infla mMatory effect by suppressing COX-2, iNOS, and TNF-$\alpha$ expressions in the synovial membrane cells.