• Fisheries and Aquatic Sciences
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• v.22 no.8
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• pp.17.1-17.9
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• 2019

Bacillus is a diverse genus consisting of more than 200 species with extensive genetic diversity. Their beneficial effects in industrial shrimp farming have been well documented. However, little is known about the biodiversity of the Bacillus spp. in this aquaculture system. Taxonomic analysis by 16S rRNA sequencing does not always allow species-level identification of Bacillus spp. In this study, 26 Bacillus isolates from two industrial Litopenaeus vannamei shrimp ponds in Bac Lieu Province, Vietnam, were analyzed for their genetic diversity by multi-locus sequence typing (MLST). A total of 22 sequence types were identified and segregated into four distinct clusters, corresponding to B. subtilis, B. velezensis, B. siamensis, and B. licheniformis. Bacillus subtilis and B. velezensis accounted for more than 73% of the Bacillus isolates. Notably, the MLST scheme exhibited high discriminatory power and might be further simplified to be a convenient method to identify species of the genus Bacillus.

• Journal of Soil and Groundwater Environment
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• v.20 no.7
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• pp.34-42
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• 2015

Feasibility tests for the hydraulic fracturing were conducted in order to secure additional saline groundwater for irrigating to the land-based aquaculture farm. Two boreholes were placed to the aquaculture farm A and B, respectively. A hydraulic fracturing using single packer was applied to major fracture zones within two boreholes. To identify effects of hydraulic fracturing on securing additional saline groundwater, some selective methods including well logging methods, pumping tests, and groundwater quality analysis were commonly applied to the boreholes before and after the hydraulic fracturing. Enlarging/creating fracture zones, increasing water contents in bedrock near boreholes, and increasing transmissivity were observed after the hydraulic fracturing. Even though the hydraulic fracturing could be an alternative to secure additional saline groundwater to the land-based aquaculture farm, salinity of the groundwater did not meet optimal thresholds for each fingerling in two farms: Fresh submarine groundwater discharge flowed the more into borehole of the farm A that resulted in decreasing a salinity value. Increased saline groundwater quantity in the borehole of the farm B rarely affect to the salinity. Although salinity problem of groundwater limited its direct use for the farms, the mixing with seawater could be effectively used for the fingerlings during the early stage. A horizontal radial collector well placed in the alluvial layer could be an alternative for the farms as well.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2010.04a
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• pp.26-27
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• 2010

Illegal aquaculture farms that cause marine pollution and ship accidents are managed and controlled by local organizations, but they experience many difficulties due to the vast waters and insufficient human resources. This study aims to utilize new IP-RFID technology to propose an efficient aquaculture farm facility management system This system attaches Ip-Tags to aquaculture farms that can provide facility information and location in real-time through IP communications. This allows for efficient management of facilities and the restriction of illegal farms. This system can also provide locations of farm facilities that sink to the ocean floor, reducing marine pollution caused by lost farms.

• Proceedings of the KSRS Conference
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• 2008.10a
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• pp.320-323
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• 2008

This study attempts to establish a system extracting and monitoring cultural grounds of seaweeds (lavers, brown seaweeds and seaweed fulvescens) and abalone on the basis of both KOMPSAT-2 and Terrasar-X data. The study areas are located in the northwest and southwest coast of South Korea, famous for coastal cultural grounds. The northwest site is in a high tidal range area (on the average, 6.1 min Asan Bay) and has laver cultural grounds for the most. An semi-automatic detection system of laver facilities is described and assessed for spacebome optic images. On the other hand, the southwest cost is most famous for seaweeds. Aquaculture facilities, which cover extensive portions of this area, can be subdivided into three major groups: brown seaweeds, capsosiphon fulvescens and abalone farms. The study is based on interpretation of optic and SAR satellite data and a detailed image analysis procedure is described here. On May 25 and June 2, 2008 the TerraSAR-X radar satellite took some images of the area. SAR data are unique for mapping those farms. In case of abalone farms, the backscatters from surrounding dykes allows for recognition and separation of abalone ponds from all other water-covered surfaces. But identification of seaweeds such as laver, brown seaweeds and seaweed fulvescens depends on the dampening effect due to the presence of the facilities and is a complex task because objects that resemble seaweeds frequently occur, particularly in low wind or tidal conditions. Lastly, fusion of SAR and optic spatial images is tested to enhance the detection of aquaculture facilities by using the panchromatic image with spatial resolution 1 meter and the corresponding multi-spectral, with spatial resolution 4 meters and 4 spectrum bands, from KOMPSAT-2. The mapping accuracy achieved for farms will be estimated and discussed after field verification of preliminary results.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.51 no.3
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• pp.333-339
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• 2015

Fish exportation using airplanes incurs sizable logistics costs (12,000KRW/kg), according for more than 45% out of the total export cost. Thus, it is unreasonable to quantitatively expand fish exportation by means of air transport. In addition, cases of failing to deliver fish at the right time to the right place occurs frequently due to the limited cargo capacity and insufficient cargo space of airplanes, especially during the peak season. Therefor, a technology that not only minimizes the logistics costs but also transports fish freshly and safely, in the case of long distance exportation to countries such as the United States and Taiwan, should be developed. In this study, a live fish container control system for long distance transportation was designed and implemented. Live flatfish (2,000kg) were selected as the target fish, were transported to the United States to analyze and verify the performance of the a live fish container control ystem and transportation ability.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.71-71
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• 2003

This study was conducted to investigate effects of dietary pigment sources on shell color of juvenile abalone, Haliotis discus hannai. Three replicate groups of the abalone, average weight 173 mg, were fed the diets containing various pigment sources such as Porphyra powder, Spirulina, yeast astaxanthin and paprika extract for 16 weeks. Survival and weight gain were not affected by dietary pigment sources (P>0.05). Shell color of abalone fed diets containing Porphyra powder and Spirulina was approached to yellow-red and orange which are similar to shell color of wild abalone. However, shell color of abalone fed the diets containing yeast astaxanthin and paprika extract were similar to that of control diet showing bright green. Porphyra powder and Spirulina contain not only fat-soluble pigment such as chlorophyll and carotenoids but also water-soluble pigment such as phycoerythrin and phycocyanin. These results would be useful information to change shell color of abalone in aquaculture.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2002.05a
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• pp.281-282
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• 2002

Out of 20 primers, 6 generated a total of 1,111 major and minor RAPD bands, producing approximately 4.2 average polymorphic bands per primer in shortnecked clam (Ruditapes philippinarum) population from Anmyeondo. The Bandsharing value altered from 0.15 to 0.74, with the average f 0.51, as calculated by bandsharing analysis. The RAPD profiles obtained with DNAs of two populations from Anmyeondo and Seocheon, respectively, were considerably different (0.20 and 0.51, respectively). The varying degrees of difference among populations amy also be of relevance to the restricted hybridization of wild bivalve. Besides gene mapping and breeding applications, PCR-RAPD systems could be very useful for the rapid certification and quality control of seed production and for every projects based on PCR amplification of specific bivalve DNA fragments.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.10a
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• pp.243-243
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• 2000

There are a large number of researches concerning about artificial reefs in Japan, and many Japanese companies have developed and specialized in the coastal engineering in recent years. Various shape, size and material of artificial reefs are constructed, however, concrete and steel structures are popular material in Japan. Five kinds of artificial reefs were put down on gravel bottoms 5-10 m deep in February 1999, which is located 100 m off Ikata, Shikoku, southern part of Japan. (omitted)

• Development and Reproduction
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• v.19 no.4
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• pp.253-258
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• 2015

Spermiation was stimulated in the mature grass puffer, Takifugu niphobles, with an injection of human chorionic gonadotropin (HCG) or carp pituitary extract (CPE). Spermatocrit and sperm density were reduced, but milt production was increased in both the HCG and CPE treatment groups relative to those in the control group (P < 0.05). These results should be useful for increasing the fertilization efficiency in grass puffer breeding programs.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.497-498
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• 2000

The venus clam, Ruditapes philippinarum, is an aquaculture shellfish mainly distributed in an intertidal zone of East Asia including Korea, China and Japan. The morphological variation of this species is great. In fact, two of the most popular markers used in molecular evolution, mitochondrial DNA (mtDNA) and nuclear ribosomal DNA (rDNA), have quite different properties, which could translate into different consequences of mutation, drift, migration and selection on patterns of geographical variation and molecular divergence. (omitted)