DNA Profiles Analysed by Polymerase Chain Reaction-Randorn Amplified Polymorphic DNAs in Shortnecked Clam (Ruditapes philippinarum) Populations

Out of 20 primers, 6 generated a total of 1,111 major and minor RAPD bands, producing approximately 4.2 average polymorphic bands per primer in shortnecked clam (Ruditapes philippinarum) population from Anmyeondo. The Bandsharing value altered from 0.15 to 0.74, with the average f 0.51, as calculated by bandsharing analysis. The RAPD profiles obtained with DNAs of two populations from Anmyeondo and Seocheon, respectively, were considerably different (0.20 and 0.51, respectively). The varying degrees of difference among populations amy also be of relevance to the restricted hybridization of wild bivalve. Besides gene mapping and breeding applications, PCR-RAPD systems could be very useful for the rapid certification and quality control of seed production and for every projects based on PCR amplification of specific bivalve DNA fragments.