• Journal of Food Hygiene and Safety
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• v.14 no.4
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• pp.319-326
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• 1999

Recently, it is continuously rising to concern about the health risk being induced by microorganisms in food such as Escherichia coli O157:H7 and Listeria monocytogenes. Various organizations and regulatory agencies including U.S.FPA, U.S.DA and FAO/WHO are preparing the methodology building to apply microbial quantitative risk assessment to risk-based food safety program. Microbial risks are primarily the result of single exposure and its health impacts are immediate and serious. Therefore, the methodology of risk assessment differs from that of chemical risk assessment. Microbial quantitative risk assessment consists of tow steps; hazard identification, exposure assessment, dose-response assessment and risk characterization. Hazard identification is accomplished by observing and defining the types of adverse health effects in humans associated with exposure to foodborne agents. Epidemiological evidence which links the various disease with the particular exposure route is an important component of this identification. Exposure assessment includes the quantification of microbial exposure regarding the dynamics of microbial growth in food processing, transport, packaging and specific time-temperature conditions at various points from animal production to consumption. Dose-response assessment is the process characterizing dose-response correlation between microbial exposure and disease incidence. Unlike chemical carcinogens, the dose-response assessment for microbial pathogens has not focused on animal models for extrapolation to humans. Risk characterization links the exposure assessment and dose-response assessment and involve uncertainty analysis. The methodology of microbial dose-response assessment is classified as nonthreshold and thresh-old approach. The nonthreshold model have assumption that one organism is capable of producing an infection if it arrives at an appropriate site and organism have independence. Recently, the Exponential, Beta-poission, Gompertz, and Gamma-weibull models are using as nonthreshold model. The Log-normal and Log-logistic models are using as threshold model. The threshold has the assumption that a toxicant is produce by interaction of organisms. In this study, it was reviewed detailed process including risk value using model parameter and microbial exposure dose. Also this study suggested model application methodology in field of exposure assessment using assumed food microbial data(NaCl, water activity, temperature, pH, etc.) and the commercially used Food MicroModel. We recognized that human volunteer data to the healthy man are preferred rather than epidemiological data fur obtaining exact dose-response data. But, the foreign agencies are studying the characterization of correlation between human and animal. For the comparison of differences to the population sensitivity: it must be executed domestic study such as the establishment of dose-response data to the Korean volunteer by each microbial and microbial exposure assessment in food.

• The Korean Journal of Food And Nutrition
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• v.15 no.2
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• pp.112-118
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• 2002

Sporulation in the fission yeast Schizosaccharomyces pombe has been regarded as an important model of cellular development and differentiation. S. pombe cells proliferate by mitosis and binary fission on growth medium. Deprivation of nutrients especially nitrogen sources, causes the cessation of mitosis and initiates sexual reproduction by matting between two sexually compatible cell types. Meiosis is then followed in a diploid cell in the absence of nitrogen source. DNA fragment complemented with the mutations of sporulation gene was isolated from the S. pombe gene library constructed in the vector, pDB 248' and designated as pDB(spo5)1. We futher analyzed six recombinant plasmids, pDB(spo5)2, pDB(spo5)3, pDB(spo5)4, pDB(spo5)5, pDB (spo5)6, pDB(spo5)7 and found each of these plasmids is able to rescue the spo5-2, spo5-3, spo5-4, spo5-5, spo5-6, spo5-7 mutations, respectively. Mapping of the integrated plasmid into the homologous site of the S. pombe chromosomes demonstrated that pDB(spo5)1, and pDB(spu5)Rl contained the spo5 gene. Transcripts of spo5 gene were analyzed by Northern hybridization. Two transcripts of 3.2 kb and 2.5kb were detected with 5kb Hind Ⅲ fragment containing a part of the spo5 gene as a probe. The small mRNA(2.5kb) appeared only when a wild-type strain was cultured in the absence of nitrogen source in which condition the large mRNA (3.2kb) was produced constitutively. Appearance of a 2.5kb spo5-mRNA depends upon the function of the meil, mei2 and mei3 genes.

• Journal of Environmental Impact Assessment
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• v.28 no.5
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• pp.492-506
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• 2019

Heavy metal contaminants in the shooting range are mostly present in a warhead circle or a metal fragment present as a particle, these fine metal particles are weathered for a long period of time is very likely that the surface is present as an oxide or carbon oxide. In particular, lead which is a representative contaminant in the shooting range soil, is present as more fine particles because it increases the softness and is stretched well. Therefore, by physical washing experiment, we conducted a degree analysis, concentration of heavy metals by cubic diameter, composition analysis of metallic substances, and assessment of applicability of gravity, magnetism and floating selection. The experimental results FESEM analysis and the measurement results lead to the micro-balance was confirmed thatthe weight goes outless than the soil ofthe same size in a thinly sliced and side-shaped structure according to the dull characteristics it was confirmed that the high specific gravity applicability. In addition, the remediation efficiency evaluation results using a hydrocyclone applied to this showed a cumulative remediation efficiency of 71%,twice 80%, 3 times 91%. On the other hand, magnetic sifting showed a low efficiency of 17%,floating selection -35mesh (0.5mm)target soil showed a relatively high efficiency to 39% -10mesh (2mm) efficiency was only 16%. The target treatment diameter of soil washing should be 2mm to 0.075mm, which is applied to the actual equipment by adding an additional input classification, which would require management as additional installation costs and processes are constructed. As a result, it is found that the soilremediation of shooting range can be separately according to the size of the warhead. The size is larger than the gravel diameter to most 5.56mm, so it is possible to select a specific gravity using a high gravity. However, the contaminants present in the metal fragments were found to be processed by separating using a hydrocyclone of the soil washing according to the weight is less than the soil of the same particle size in a thinly fragmented structure.

• Journal of The Korean Association For Science Education
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• v.19 no.3
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• pp.428-440
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• 1999

The colorful overhead projector films, named as Bio-cosmos II, including photographs, pictures, concept maps, and diagrams, were developed and manufactured as audio-visual teaching aids and teaching resources for students' biology learning in high school, and the CD-ROM and web sites for their application to the school were also constructed. The content of the films was organized based upon the analysis of seven different biology textbooks approved by the Ministry of Education. The films were designated based on various instructional strategies and manufactured using multimedia with various educational softwares. The CD-ROM was composed of the scenes as logo, initial main, chapters list, contents, and quit. Initial main scene indicated various chapters according to the texts of biology areas in General Science, Biology I, and II. Each chapters linked with the scenes for detailed concept maps, the downstream real subjects, and contents. The subject screens were composed of various types of summarized diagrams including lesson contents, figures, pictures, photographs, and their explanation, experimental procedures and results, tables for summarized contents, and additional animation with video captures, explanations, glossary, etc. Most files were manufactured in software Adobe Photoshop by scanning the pictures, figures and photographs, and then the explanation, modification, storing with PICT or PSD files, and transformation with JPG files, were processed in the aspect of high quality in terms of instructional strategies and graphic skills on gracefulness, clearness, colorfulness, brightness, and distinctness. A 14 films for biology areas in General Science, 80 for Biology I, and 142 for Biology II were manufactured and loaded to the CD-ROM and web site, and the files had been attempted to opened with an internet home-page of http://gic.kyungpook.ac.kr/biocosmos.

• Journal of the Korean Society for Marine Environment & Energy
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• v.13 no.1
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• pp.18-29
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• 2010

Offshore subsurface storage of $CO_2$ is regarded as one of the most promising options to response severe climate change. Marine geological storage of $CO_2$ is to capture $CO_2$ from major point sources, to transport to the storage sites and to store $CO_2$ into the offshore subsurface geological structure such as the depleted gas reservoir and deep sea saline aquifer. Since 2005, we have developed relevant technologies for marine geological storage of $CO_2$. Those technologies include possible storage site surveys and basic designs for $CO_2$ transport and storage processes. To design a reliable $CO_2$ marine geological storage system, we devised a hypothetical scenario and used a numerical simulation tool to study its detailed processes. The process of transport $CO_2$ from the onshore capture sites to the offshore storage sites can be simulated with a thermodynamic equation of state. Before going to main calculation of process design, we compared and analyzed the relevant equation of states. To evaluate the predictive accuracies of the examined equation of states, we compare the results of numerical calculations with experimental reference data. Up to now, process design for this $CO_2$ marine geological storage has been carried out mainly on pure $CO_2$. Unfortunately the captured $CO_2$ mixture contains many impurities such as $N_2$, $O_2$, Ar, $H_{2}O$, $SO_{\chi}$, $H_{2}S$. A small amount of impurities can change the thermodynamic properties and then significantly affect the compression, purification and transport processes. This paper analyzes the major design parameters that are useful for constructing onshore and offshore $CO_2$ transport systems. On the basis of a parametric study of the hypothetical scenario, we suggest relevant variation ranges for the design parameters, particularly the flow rate, diameter, temperature, and pressure.

• Economic and Environmental Geology
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• v.57 no.4
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• pp.397-416
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• 2024

This study assessed the hydrogeological properties of the deep geological environment to develop safety criteria for the natural barriers used in the deep geological disposal of high-level radioactive waste in Korea. The assessment focused on the distribution and trends of hydraulic conductivity and permeability properties appropriate for the domestic geological environment, using various in-situ hydraulic test data collected for groundwater development and management. To develop a depth-hydrogeological property relationship model suitable for domestic conditions, the study reviewed various international research examples and applied a representative model that explains the trends of hydraulic conductivity and permeability with depth. The development of the model suitable for Korea involved applying ensemble regression analysis to account for the uncertainty of various factors in the collected data. The results confirmed that existing international depth-hydrogeological property relationship models adequately describe the characteristics of the domestic geological environment. Considering the preferred hydrogeological criteria suggested by countries like Sweden, Germany, and Canada, there is a high likelihood that a suitable geological environment exists in Korea. Additionally, the application of hydrogeological criteria indicative of low-permeability environments showed that suitable conditions for disposal construction increase at depths greater than 300 m, where the influence of fractures on groundwater flow might be minimal at depths exceeding 500 m. This research can serve as foundational information for establishing hydrogeological safety standards for natural barriers in Korea according to international regulatory guidelines.

• Journal of Korean Tunnelling and Underground Space Association
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• v.2 no.2
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• pp.11-21
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• 2000

Tunnelling has been created as a great extent in view of less land space available because the growth of population in metropolitan has been accelerated at a faster pace than the development of the cities. In tunnelling, it is often faced that measures are obliged to be taken without confirmation for such abnormality as diverged movement of surrounding rock mass, growing crack of shotcrete and yielding of rockbolts. In this case, it is usually said that the judgments of experienced engineers for the selection of measure are importance and allowed us to get over the situations in many construction sites. But decrease of such experienced engineers need us to develop the new system to assist the selection of measures for the abnormality without any experiences of similar tunnelling sites. In this study, After a lot of tunnelling reinforcement methods were surveyed and the detail application were studied, an expert system was developed to predict the safety of tunnel and choose proper tunnel reinforcement system using fuzzy quantification theory and fuzzy inference rule based on tunnel information database. The expert system developed in this study have two main parts named pre-module and post-module. Pre-module decides tunnel information imput items based on the tunnel face mapping information which can be easily obtained in-situ site. Then, using fuzzy quantification theory II, fuzzy membership function is composed and tunnel safety level is inferred through this membership function. The comparison result between the predicted reinforcement system level and measured ones was very similar. In-situ data were obtained in three tunnel sites including subway tunnel under Han river. This system will be very helpful to make the most of in-situ data and suggest proper applicability of tunnel reinforcement system developing more resonable tunnel support method from dependance of some experienced experts for the absent of guide.

• Journal of Soil and Groundwater Environment
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• v.13 no.5
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• pp.81-87
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• 2008

In accompany with the transfer of US army bases, recent surveys reported serious contamination of soils by the release of petroleum from storage facilities and heavy metals accumulated in rifle-ranges. These problems have made an increased concerns of cleanup technology for contaminated soils. In this study, a full-scale soil washing process improved by multistage continuous desorption and agitational desorption techniques was examined for petroleum-contaminated soils obtained from three different remedial sites that contained 29.3, 16.6, and 7.8% of silt and clay, respectively. The initial concentrations of total petroleum hydrocarbon (TPH) were 5,183, 2,560, and 4,860 mg/kg for each soil. Pure water was applied to operate washing process, in which water used for washing process was recycled 100% for over 6 months. The results of full-scale washing tests showed that the TPH concentrations for soils (> 3.0 mm) were 50${\sim}$356 mg/kg (85.2${\sim}$98.2% removal rates), regardless of the contents of silt and clay from in A, B and C soil, when the soils were washed at 3.0 kg/$cm^2$ of injection pressure with the method of wet particle separation. Based on the initial TPH concentration, the TPH removal rates for each site were 85.2, 98.2 and 89.9%. For soils in the range of 3.0${\sim}$0.075 mm, the application of first-stage desorption technique as a physical method resulted 834, 1,110, and 1,460 mg/kg of TPH concentrations for each soil, also additional multi-stage continuous desorption reduced the TPH concentration to 330, 385, and 245 mg/kg that were equivalent to 92.4, 90.6, and 90.1% removal rates, respectively. The result of multi-stage continuous desorption for fine soil (0.075${\sim}$0.053 mm) were 791, 885, and 1,560 mg/kg, and additional agitation desorption showed 428, 440, and, 358 mg/kg of TPH concentrations. Compared with initial concentration, the removal rates were 92.0, 93.9 and 92.9%, respectively. These results implied we could apply strategic process of soil washing for varies types of contaminated soils to meet the regulatory limit of TPH.

• Journal of the Korean Society for Marine Environment & Energy
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• v.11 no.1
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• pp.24-34
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• 2008

To mitigate the climate change and global warming, various technologies have been internationally proposed for reducing greenhouse gas emissions. Especially, in recent, carbon dioxide capture and storage (CCS) technology is regarded as one of the most promising emission reduction options that $CO_2$ be captured from major point sources (eg., power plant) and transported for storage into the marine geological structure such as deep sea saline aquifer. The purpose of this paper is to review the latest progress on the development of technologies for $CO_2$ storage in marine geological structure and its perspective in republic of Korea. To develop the technologies for $CO_2$ storage in marine geological structure, we carried out relevant R&D project, which cover the initial survey of potentially suitable marine geological structure fur $CO_2$ storage site and monitoring of the stored $CO_2$ behavior, basic design for $CO_2$ transport and storage process including onshore/offshore plant and assessment of potential environmental risk related to $CO_2$ storage in geological structure in republic of Korea. By using the results of the present researches, we can contribute to understanding not only how commercial scale (about 1 $MtCO_2$) deployment of $CO_2$ storage in the marine geological structure of East Sea, Korea, is realized but also how more reliable and safe CCS is achieved. The present study also suggests that it is possible to reduce environmental cost (about 2 trillion Won per year) with developed technology for $CO_2$ storage in marine geological structure until 2050.

• Korean Journal of Agricultural and Forest Meteorology
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• v.17 no.1
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• pp.15-24
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• 2015

$CH_4$ is a trace gas and one of the key greenhouse gases, which requires continuous and systematic monitoring. The application of eddy covariance technique for $CH_4$ flux measurement requires a fast-response, laser-based spectroscopy. The eddy covariance measurements have been used to monitor $CO_2$ fluxes and their data processing procedures have been standardized and well documented. However, such processes for $CH_4$ fluxes are still lacking. In this note, we report the first measurement of $CH_4$ flux in a rice paddy by employing the eddy covariance technique with a recently commercialized wavelength modulation spectroscopy. $CH_4$ fluxes were measured for five consecutive days before and after the rice transplanting at the Gimje flux monitoring site in 2012. The commercially available $EddyPro^{TM}$ program was used to process these data, following the KoFlux protocol for data-processing. In this process, we quantified and documented the effects of three key corrections: (1) frequency response correction, (2) air density correction, and (3) spectroscopic correction. The effects of these corrections were different between daytime and nighttime, and their magnitudes were greater with larger $CH_4$ fluxes. Overall, the magnitude of $CH_4$ flux increased on average by 20-25% after the corrections. The National Center for AgroMeteorology (www.ncam.kr) will soon release an updated KoFlux program to public users, which includes the spectroscopic correction and the gap-filling of $CH_4$ flux.