• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.184-184
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• 2005

• Journal of Plant Biology
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• v.29 no.1
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• pp.11-18
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• 1986

Protopasts were isolated from cultured cells of potato (Solanum tuberosum L.) tuber tissue. The ability of callus formation from the culture cells was higher in cultivars Dejima and Superior than in Shimabara and Irish Cobbler on Lam's medium. Therefore, the former was used as sources for protoplast isolation. Friable calli were transferred to liquid media and cells in exponential phase were used for protoplast isolation. In both of Dejima and Superior, the yield of protoplasts was high in the enzyme solution of 2% Onozuka cellulase and 1% macerozyme. Also, viability of isolated protoplasts was very good. Thus, it seems that these protoplasts would be applicable to various aims of research.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.48-48
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• 2020

Assessment of plant genetic resources is applicable when breeding improved plant caltivars. In Korea, early maturing wheat germplasm is anticipated for wheat-rice double-cropping. In this study, we assessed five agronomical traits including days of heading after sowing(HD), days of maturity after sowing(MD), culm length(CL), ear length(EL), and thousand grain weight(TW) of 500 wheat germplasms collected form 10 different countries (Mexico, the United States, Afghanistan, Ethiopia, India, Japan, China, Turkey, Pakistan and Portugal), and grown in Korea. Besides, the correlation between the agronomical traits was analyzed using XLSTAT softerware version 2019 (Addinsoft, NY, USA). The result showed wide-ranging maturity period. Among the entire population, 2 accessions (K256306/JPN/breeding line and K256328/JPN/breeding line) that matured early were identified. Furthermore, HD showed strong correlation with MD (r=0.684) and CL (r=0.610), and weak correlation with the rest two agronomical traits (EL and TW). Overall, the results of our study provides wide spectrum of prospects, and could be applicable to breed new wheat varieties with early maturity.

• Journal of Biosystems Engineering
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• v.23 no.6
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• pp.629-634
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• 1998

Factory-style plant production system aims to produce the standardized horticultural products with high quality and cleanness. In Korea, researches for year-round leaf vegetables production system are in progress and the most of them are focused on environment control. Automating technologies for harvesting, transporting and grading need to be developed. A lettuce harvesting system applicable to the plant factory was studied. It was composed of an articulated robot with a cutter and a gripper, lettuce feeding conveyor and air blower. Success rate of the developed system was 94.7 %. The system carried out harvesting a lettuce smoothly and the harvesting time was about 6 seconds per lettuce. The results showed a feasibility of robotic lettuce harvesting.

• Plant Journal
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• v.6 no.1
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• pp.56-63
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• 2010

While the local plant market is reducing its volume, the plant market over the world since 2000s is rapidly expanding. The nation's construction companies, aggressively dedicated in launching out overseas plant market, increase the volume of orders in that sector, but there also are much difficulty in the project management as those projects ordered are gradually large scaled with more cutting-edge high-tech requirements along with comparatively higher risk. Though the local construction companies have developed their own types of measures to analyze the risk evaluation putting into practice, the specialized decision-making model for the overseas plant market or the risk measure understandable easily and applicable practically is not yet shown. This paper aims at providing the methodology to evaluate the risk by way of constructing the risk evaluation process in order to induce risk measuring elements through appropriate indexing system. Furthermore, through studying the risk management system, it aims to seek for a thorough risk management method from beginning of the project to the end.

• The Plant Pathology Journal
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• v.21 no.3
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• pp.288-292
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• 2005

Pathogenesis-related protein-1a (PR-1a) is strongly induced in tobacco plants by pathogen attack, exogenous salicylic acid (SA) application and by other developmental processes. In order to develop a rapid screening system for the selection of plant defense-inducing compounds originated from various sources, we have transformed tobacco Samsun NN plants with a chimeric construct consisting of GUS $(\beta-glucuronidase)$. In the $T_1$ generation, three transgenic lines having stable GUS expression were selected for further promoter analysis. Using GUS histochemical assay, we observed strong GUS induction driven by PR-1a promoter in PR1a-GUS transgenic tobacco leaves in response to the exogenous application of SA or benzol (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester (BTH), a SA­derivative compound. In addition, GUS expression was maintained locally or systemically in PR1a-GUS transgenic line $\#5\;T_2$ generation) until after 3 days when they were treated with same chemicals. Our results suggested that the PR1a-GUS reporter gene system in tobacco plants may be applicable for the large-scale screening of defense-inducing substances.

• Journal of Plant Biotechnology
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• v.46 no.2
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• pp.114-118
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• 2019

Efficient protocol for plant shoot regeneration of Brassica juncea L. CZERN was established by using organic media components and growth stimulating factors of the vermicompost and coelomic fluids. Formulated organic plant tissue culture media (Vermicompost (30%) extracts supplemented with 20 mL/L coelomic fluid) have shown maximum shoot regeneration when compared with the Murashige and Skoog (MS) medium, which were supplemented with 1 mg/L 6-benzyladenine (BA) and 0.1 mg/L of Naphthaleneacetic acid (NAA). Cotyledon explants produced the highest shoot regeneration frequency from fourday-old germinated seedlings in comparison with non-germinated seedlings. The vermicompost extracts have proved to be the best organic plant growth media to induce shoots from cotyledons compared to the MS media. Statistically significant difference (P = 0.008) for the root length, shoot length (P=0.000350) and the leaves (P=0.375) of the mustard plantlets were analyzed successfully. The survival rate was 98% in the mustard cotyledons on the Vermicompost extract media and 63% on MS media respectively. The coelomic fluid also is much suitable to induce shoots from cotyledons at lower concentrations. It was also shown that the vermicompost extract, which comprised of humic acids along with coelomic fluid, affected shoot regeneration from the cotyledons. An efficient and organic shoot regeneration study was standardized and it can be applicable in the improvement of the economically important crops.

• Economic and Environmental Geology
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• v.28 no.5
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• pp.455-467
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• 1995

In order to investigate the dispersion patterns and speciations of Cu, Pb, Zn and Cd in soils, stream sediments and stream waters, geochemical studies of soil, stream sediment and stream water samples collected in the vicinity of the Shi-Heung Cu-Pb-Zn mine was carried out Cation exchange capacity measurement, size analysis, X-ray diffraction analysis and batch test were performed to select applicable soil for adsorption treatment The average content of Cu, Pb, Zn and Cd in soils collected from tailings and ore dressing plant is 1084 ppm, 2292 ppm, 3512 ppm and 29.2 ppm, respectively, and therefore, tailings and ore dressing plant site may be the major contamination sources in this study area. The mean content of Cu, Pb, Zn and Cd in stream sediments is extremely high up to 794 ppm, 1633 ppm, 2946 ppm and 25.2 ppm, respectively. Tailing particles and heavy metal ions are dispersed along the tributary system. Results from the sequential extraction analysis indicate; (1) most of Cu is bound to organic matters and sulphides, (2) fraction of Pb is mainly bound to Fe and Mn oxides. Most of Zn is largely bound to Fe and Mn oxides and residual fraction. Ion exchangeable fraction of Cd is relatively higher than those of Cu, Pb and Zn. Batch test on soils collected from the kaolinite and/or pyrophyllite mines and from the control areas was carried out to select an applicable soil samples for adsorption treatment The sample, S10, collected from the control area 2 (clay content 33.2%) shows the highest $K_d$ (distribution coefficient). Organic content in soils and several clay minerals shows relatively good correlation with $K_d$. It means that applicable soils for adsorption treatment of heavy metals show high organic and clay content.

• The Transactions of the Korean Institute of Electrical Engineers A
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• v.48 no.6
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• pp.760-772
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• 1999

The CAN is a serial communication protocol for distributed real-time control and automation systems. Data generated from field devices in the distributed control of power plant are classified into three categories: real-time event data, real-time control data, non-real-time data. These data share a CAN medium. If the traffic of the CAN protocol is not efficiently controlled, performance requirements of the power plant system could not be satisfied. This paper proposes a bandwidth allocation algorithm that can be applicable to the CAN protocol. The bandwidth allocation algorithm not only satisfies the performance requirements of the real-time systems in the power plant but also fully utilizes the bandwidth of CAN. The bandwidth allocation algorithm introduced in this paper is validated using the integrated discrete-event/continuous-time simulation model which comprises the CAN network and distributed control system of power plant.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.6 no.6
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• pp.974-979
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• 2002

There are so many kind of a control program that is applied in various process fields such as power generation plant, water treatment plant, incinerator plant, chemical plant, cement plant etc.. Because an engineer in field edits and changes and debugs and tests properly control programs using text-based control language, it is very hard for the him to apply to plant. Therefore, this research implemented a graphical tool for control program builder that is applicable to various plants and usable engineers having a little knowledge for control language. I wish to run more efficiently precision process control offering function that can see visual expression about flow of control signal and intermediate output values of control program displayed in screen using this implemented function block builder.