DOI QR코드

DOI QR Code

Influence of formulated organic Plant tissue culture medium in the shoot regeneration study of Brassica juncea (l.) - Indian mustard

  • Received : 2019.03.18
  • Accepted : 2019.06.14
  • Published : 2019.06.30

Abstract

Efficient protocol for plant shoot regeneration of Brassica juncea L. CZERN was established by using organic media components and growth stimulating factors of the vermicompost and coelomic fluids. Formulated organic plant tissue culture media (Vermicompost (30%) extracts supplemented with 20 mL/L coelomic fluid) have shown maximum shoot regeneration when compared with the Murashige and Skoog (MS) medium, which were supplemented with 1 mg/L 6-benzyladenine (BA) and 0.1 mg/L of Naphthaleneacetic acid (NAA). Cotyledon explants produced the highest shoot regeneration frequency from fourday-old germinated seedlings in comparison with non-germinated seedlings. The vermicompost extracts have proved to be the best organic plant growth media to induce shoots from cotyledons compared to the MS media. Statistically significant difference (P = 0.008) for the root length, shoot length (P=0.000350) and the leaves (P=0.375) of the mustard plantlets were analyzed successfully. The survival rate was 98% in the mustard cotyledons on the Vermicompost extract media and 63% on MS media respectively. The coelomic fluid also is much suitable to induce shoots from cotyledons at lower concentrations. It was also shown that the vermicompost extract, which comprised of humic acids along with coelomic fluid, affected shoot regeneration from the cotyledons. An efficient and organic shoot regeneration study was standardized and it can be applicable in the improvement of the economically important crops.

Keywords

References

  1. Cao J, Earle ED (2003) Transgene expression in broccoli (Brassica oleracea var. italica) clones progated in vitro leaf explants. Plant Cell Rep 21:789-796 https://doi.org/10.1007/s00299-003-0589-6
  2. Chi GL, Barfield DG, Sim GE, Pua EC (1990) Effect of silver nitrate and aminoethoxyvinylglycine on in vitro shoot and root organogenesis from seedling explants of recalcitrant Brassica genotypes. Plant Cell Rep 9:195-198 https://doi.org/10.1007/BF00232178
  3. Eapen S, George L (1997) Plant regeneration from peduncle segments of oil seed Brassica species: influence of silver nitrate and silver thiosulfate. Plant Cell Tiss. Org. Cult 51:229-232 https://doi.org/10.1023/A:1005926108586
  4. Frankenberger WT, Arshad M (1995) Phytohormones in Soils: Microbial Production and Function. Marcel and Deckker Pub., New York, pp 503
  5. Hemingway JS (1976) Mustard: Brassica spp. and Sinapis alba (Cruciferae). pp 56-9. (in) Evolution of Crop Plants. Simmonds (Ed.). Longmans, London.
  6. Kim MS, Schumann CM, Klopfenstein NB (1997) Effects of thidiazuron and benzylaminopurine on axillary shoot proliferation of three green ash (Fraxinus pennsylvanica Marsh.) clones. Plant Cell Tiss. Org. Cult 48:45-52 https://doi.org/10.1023/A:1005856720650
  7. Mathews VH, Bhatia CR, Mitra R, Krishna TG, Rao PS. (1985) Regeneration of shoots from Brassica juncea (Linn.) Czern and Coss cells transformed by Agrobacterium tumefaciens and expression of nopaline dehydrogenase genes. Plant Sci 39:49-54 https://doi.org/10.1016/0168-9452(85)90191-8
  8. Mathews VH, Bharathan N, Litz RZ, Rao PS, Bhatia CR (1990) Transgenic plants of mustard Brassica juncea L. czern and coss. Plant Sci 72:245-252 https://doi.org/10.1016/0168-9452(90)90088-6
  9. Metz TD, Dixit R, Earle ED (1995) Agrobacterium tumefaciens-mediated transformation of broccoli (Brassica oleracea var. italica) and cabbage (B. oleracea var. capitata). Plant Cell Rep 15:287-292 https://doi.org/10.1007/BF00193738
  10. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacoo tissue culture. Physiol. Plant. 15:473-495 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  11. Sinha K, Rajiv, Herat Sunil, Valani, Dalsukhbhai, Chauhan, Krunalkumar (2009) Earthworms Vermicompost: A Powerful Crop Nutrient over the Conventional Compost & Protective Soil Conditioner against the Destructive Chemical Fertilizers for Food Safety and Security, American-Eurasian Journal of Agricultural & Environmental Sciences, pp 1-55
  12. Tomati U, Grappelli A, Galli E (1988) The hormone-like effect of earthworm casts on plant growth. Biol Fertil Soils 5:288-294 https://doi.org/10.1007/BF00262133
  13. Wahlroos T, Susi P, Tylkina L, Malyshenko S, Zvereva S, Korpela T (2003) Agrobacterium- mediated transformation and stable expression of the green fluorescent protein in Brassic arapa. Plant Physiol Biochem 41:773-778 https://doi.org/10.1016/S0981-9428(03)00119-0