• Journal of Plant Biotechnology
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• v.32 no.3
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• pp.151-159
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• 2005

Injury caused by reactive oxygen species (ROS), known as oxidative stress, is one of the major damaging factors in plants exposed to environmental stress. Chloroplasts are specially sensitive to damage by ROS because electrons that escape from the photosynthetic electron transfer system are able to react with relatively high concentration of $O_2$ in chloroplasts. To cope with oxidative stress, plants have evolved an efficient ROS-scavenging enzymes such as superoxide dismutase (SOD) and ascorbate peroxidase (APX), and low molecular weight antioxidants including ascorbate, glutathione and phenolic compounds. To maintain the productivity of plants under the stress condition, it is possible to fortify the antioxidative mechanisms in the chloroplasts by manipulating the antioxidation genes. A powerful gene expression system with an appropriate promoter is key requisite for excellent stress-tolerant plants. We developed a strong oxidative stress-inducible peroxidase (SWPA2) promoter from cultured cells of sweetpotato (Ipomoea batatas) as an industrial platform technology to develop transgenic plants with enhanced tolerance to environmental stress. Recently, in order to develop transgenic sweetpotato (tv. Yulmi) and potato (Solanum tuberosum L. cv. Atlantic and Superior) plants with enhanced tolerance to multiple stress, the genes of both CuZnSOD and APX were expressed in chloroplasts under the control of an SWPA2 promoter (referred to SSA plants). As expected, SSA sweetpotato and potato plants showed enhanced tolerance to methyl viologen-mediated oxidative stress. In addition, SSA plants showed enhanced tolerance to multiple stresses such as temperature stress, drought and sulphur dioxide. Our results strongly suggested that the rational manipulation of antioxidative mechanism in chloroplasts will be applicable to the development of all plant species with enhanced tolerance to multiple environmental stresses to contribute in solving the global food and environmental problems in the 21st century.

• Korean Journal of Veterinary Service
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• v.37 no.4
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• pp.263-271
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• 2014

In this study, we developed a cost and time saving one-step multiplex RT-PCR for the simultaneous detection and differentiation of swine influenza viruses (SIV) and 2009 pandemic influenza H1N1 virus (pH1N1). The one-step multiplex RT-PCR using four sets of primer was confirmed to be capable of detection of all SIV subtypes and differential diagnosis of major SIV subtype H1, H3 and pH1N1 on individual or mixed viral culture samples. The sensitivity of the multiplex RT-PCR was determined to be at least $2^{-6}$ $HA/25{\mu}L$ of the presented SIVs, providing sufficient efficacy for a routine SIV monitoring in diagnostic laboratories. In addition, compared with the conventional RT-PCR methods that cannot avoid the carryover DNA contamination, the developed RT-PCR applied with the uracil DNA glycosylase (UNG) system was proven to prevent a false positive reaction by carryover contamination of the pre-amplified DNA. In conclusion, the one-step RT-PCR with UNG system could be applicable to detect and differentiate of SIV from the viral cultures without worry of carryover DNA contamination in clinical laboratories.

• Weed & Turfgrass Science
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• v.2 no.2
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• pp.170-175
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• 2013

Korea is carrying out weed quarantine by plant quarantine regulations including heat-treatment method to prevent the influx of exotic weeds. In order to confirm suitability for current heat-treatment criteria and find field-applicable methods which can completely devitalize quarantine weed seeds, the conditions for heat-treatment with/without 40% relative humidity were studied with 9 species among quarantine weed seeds and one weed species similar to quarantine weed. Dry heat-treatments had been tested under various temperatures and time conditions. All seeds were dead at the conditions of $95^{\circ}C$ for 48 hours, $100^{\circ}C$ for 36 hours, $110^{\circ}C$ for 24 hours, $121^{\circ}C$ for 4 hours and $130^{\circ}C$ for 30 minutes. Heat-treatments at 40% relative humidity resulted in complete seed death at the conditions of $85^{\circ}C$ for 36 hours and $90^{\circ}C$ for 16 hours. The above results show that current heat-treatment criteria are not suitable for several quarantine weeds and these conditions could be applied as a quarantine method to prevent the influx of quarantine weeds along with agricultural imports. More specific conditions for heat tolerant species such as Picris echioides and heat susceptible species such as Cuscuta spp. are necessary and will improve plant quarantine process in devitalizing quarantine weed seeds with different heat tolerances.

• Journal of Bio-Environment Control
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• v.28 no.4
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• pp.358-365
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• 2019

This study was conducted to estimate the dimensions of horticultural products and the mean plant height of plug seedlings using three-dimensional (3D) images. Two types of camera, a ToF camera and a stereo-vision camera, were used to acquire 3D images for horticultural products and plug seedlings. The errors calculated from the ToF images for dimensions of horticultural products and mean height of plug seedlings were lower than those predicted from stereo-vision images. A new indicator was defined for determining the mean plant height of plug seedlings. Except for watermelon with tap, the errors of circumference and height of horticultural products were 0.0-3.0% and 0.0-4.7%, respectively. Also, the error of mean plant height for plug seedlings was 0.0-5.5%. The results revealed that 3D images can be utilized to estimate accurately the dimensions of horticultural products and the plant height of plug seedlings. Moreover, our method is potentially applicable for segmenting objects and for removing outliers from the point cloud data based on the 3D images of horticultural crops.

• Journal of the Korea Concrete Institute
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• v.28 no.6
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• pp.703-711
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• 2016

The present study is mainly aimed at securing adequate pores which are applicable to the aquatic environment and satisfying the required strength of porous concrete as a structure by substituting pumice for crushed stone which is usually used for the fabrication of porous concrete. Accordingly, in order to deduce the optimum mixing conditions applicable to the aquatic environment, we sought to evaluate the porosity, coefficient of permeability and compressive strength of porous concrete based on the target porosity and the mixing factors for pumice. By examining the porosity and coefficient of permeability of porous concrete and the physical properties of its compressive strength based on the target porosity and the mixing factors for pumice, it is judged that the optimum mixtures for porous concrete applicable to the aquatic environment which satisfy both the necessity of securing adequate pores and the required strength for porous concrete as a structure are PC I I-10-0, PC I I-10-5 and PC I I-10-10.

• Korean Journal of Agricultural and Forest Meteorology
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• v.25 no.4
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• pp.387-397
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• 2023

Plant height is a growth parameter that provides visible insights into the plant's growth status and has a high correlation with yield, so it is widely used in crop breeding and cultivation research. Investigation of the growth characteristics of crops such as plant height has generally been conducted directly by humans using a ruler, but with the recent development of sensing and image analysis technology, research is being attempted to digitally convert growth measurement technology to efficiently investigate crop growth. In this study, the canopy height of rice grown at various nitrogen fertilization levels was measured using a laser scanner capable of precise measurement over a wide range, and a comparative analysis was performed with the actual plant height. As a result of comparing the point cloud data collected with a laser scanner and the actual plant height, it was confirmed that the estimated plant height measured based on the average height of the top 1% points showed the highest correlation with the actual plant height (R² = 0.93, RMSE = 2.73). Based on this, a linear regression equation was derived and used to convert the canopy height measured with a laser scanner to the actual plant height. The rice growth curve drawn by combining the actual and estimated plant height collected by various nitrogen fertilization conditions and growth period shows that the laser scanner-based canopy height measurement technology can be effectively utilized for assessing the plant height and growth of rice. In the future, 3D images derived from laser scanners are expected to be applicable to crop biomass estimation, plant shape analysis, etc., and can be used as a technology for digital conversion of conventional crop growth assessment methods.

• Journal of Environmental Impact Assessment
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• v.24 no.3
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• pp.260-277
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• 2015

The object of this study was the climate change sensitivity assessment of Korean Fir and Khinghan Fir as a representative subalpine plant in South Korea. Using species distribution models, we predicted the probability of current and future species distribution. According to this study, potential distribution that have been predicted based on the threshold (MTSS) is, Khinghan Fir was higher loss rate than Korean Fir. And in the climate change sensitivity assessment using the scalar sensitivity weight ($W_{is}$), $W_{is}$ of Korean Fir was higher relatively than the sensitivity of Khinghan Fir. When using the species distribution models as shown in this study may vary depending on the probability of presence data and spatial variables. Therefore should be prior decision studies on the ecological environment of the study species. Based on this study, if it is domestic applicable climate change sensitivity assessment method is developed. it would be important decision-making to climate change and biological diversity of adaptation policy.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.1859-1864
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• 2004

An effective methodology is reported for the optimal design of multisite batch production/transportation and storage networks under uncertain demand forecasting. We assume that any given storage unit can store one material type which can be purchased from suppliers, internally produced, internally consumed, transported to or from other plant sites and/or sold to customers. We further assume that a storage unit is connected to all processing and transportation stages that consume/produce or move the material to which that storage unit is dedicated. Each processing stage transforms a set of feedstock materials or intermediates into a set of products with constant conversion factors. A batch transportation process can transfer one material or multiple materials at once between plant sites. The objective for optimization is to minimize the probability averaged total cost composed of raw material procurement, processing setup, transportation setup and inventory holding costs as well as the capital costs of processing stages and storage units. A novel production and inventory analysis formulation, the PSW(Periodic Square Wave) model, provides useful expressions for the upper/lower bounds and average level of the storage inventory. The expressions for the Kuhn-Tucker conditions of the optimization problem can be reduced to two sub-problems. The first yields analytical solutions for determining lot sizes while the second is a separable concave minimization network flow subproblem whose solution yields the average material flow rates through the networks for the given demand forecast scenario. The result of this study will contribute to the optimal design and operation of large-scale supply chain system.

• Korean Journal of Plant Resources
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• v.32 no.6
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• pp.689-697
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• 2019

This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoot tips of strawberry (Fragaria × ananassa Duch.) cvs. 'Wonkyo3114' and 'Gurumi40'. The shoot tips were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.5M). Precultured explants were osmoprotected with loading solution (LS, C4) containing 20% glycerol and 20% sucrose for 40 min and exposed to dehydration solution (B5) containing 40% glycerol and 40% sucrose for 40 min at 25℃, Subsequently, the explants were transferred onto droplets containing 2.5 μL PVS3 on sterilized aluminum foils (4 cm × 0.5 cm) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regrowth rate (%) in both the cultivars was obtained when the shoot tips were precultured with MS + 0.3M sucrose for 40 h at 25℃. The cryopreserved shoots tips exhibited 55% regrowth rate by culturing in NH₄NO₃-free MS medium supplemented with 3% sucrose, 1.0 g/L casein, 1.0 mg/L GA₃, and 0.5 mg/L BA for 5 weeks and in MS medium supplemented with 0.5 mg/L GA₃ for 8 weeks. This result shows that droplet-vitrification could be employed as a promising method for cryostorage of strawberry germplasm.

• Korean Journal of Environmental Agriculture
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• v.33 no.4
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• pp.271-281
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• 2014

BACKGROUND: Application of the transfer functions derived from local soil data is necessary in order to develop proper management protocols for agricultural soils contaminated with heavy metals through phytoavailability control of the heavy metals. The aim of this study was to derive the transfer functions of Korean agricultural soils affected by the abandoned mining sites and evaluate suitability of the derived transfer functions. METHODS AND RESULTS: 142 agricultural soils affected by the abandoned mining sites were collected and analyzed. Two extraction methods, including 1 M $NH_4NO_3$ extraction and 0.01 M $Ca(NO_3)_2$ extraction were applied to determine phytoavailable metal pools in soils. Multiple stepwise regression of phytoavailable metal pools against the corresponding total metal concentration and soil properties was conducted to derive suitable transfer functions for estimating phytoavailable heavy metal pools. Applicability of the derived transfer functions was examined by calculating NME and NRMSE. CONCLUSION: Soil pH and organic matter were valid variables for derivation of the transfer functions which were applicable for estimating phytoavailable metal concentrations in the soils being contaminated by heavy metals. In addition, it was confirmed that transfer functions need to be developed based on local soil conditions to accurately estimate heavy metal-phytoavailability.