• Journal of Nutrition and Health
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• v.25 no.5
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• pp.339-350
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• 1992

This study was performed to investigate the effect on polyunsaturate fatty acid diets and feeding periods on the antithrombosis. the hematological changes in the blood and fatty acid compositions of platelets in rats. Each group of rats was fed a diet containing 20%(W/W) corn oil beef tallow sardine oil and the general stock diet for 10, 20. 40 and 80 days. Rats fed sardine oil diet showed significantly longer bleeding time than any other diet groups after 20 days feeding The whole blood clotting time of sardine oil group fed for 80 days was increased significantly. The number of platelet and the concentration of hemoglobin showed no significant difference among all groups. The number of white blood cell was decreased continously in sardine oil group after 10 days feeding. The level of malondialdehyde generation during thrombin-induced aggregation of platelets was decreased continously in sardine oil grou after 20 days feeding. With regard to the composition of platelet fatty acid the ratio of eicosapentaenoic acid(EPA 20: 5 $\omega$-3) to arachidonic acid(AA 20:4 $\omega$-6) was increased in sardine oil group but decreased in corn oil groups and beef tallow groups with days. In conclusion the rats fed sardine oil diet for more than 20 days showed the fact that EPA induced the antithrombosis. the changes in number of white blood cell and the fatty acid composition of platelets.

• Microbiology and Biotechnology Letters
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• v.42 no.3
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• pp.285-292
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• 2014

In the course of study for development of functional food ingredients from aged black garlic (ABG), heat-treated ripe bulbs of Allium sativum L., the water extracts from raw-garlic (RG) and ABG, and the subsequent organic solvent fractions of ABG were prepared, and their antioxidant, antimicrobial, and antithrombosis activities were compared. The extraction yield of ABG was 4-folds higher than that of RG, and the contents of total polyphenol, total flavonoid, total sugar and reducing sugar in the ABG extract were 4-folds, 1.56-folds, 3.36-folds and 6.75-folds higher than those of the RG extract, respectively. In antioxidation activity assay, the extract of ABG showed minor scavenging activity against DPPH anion, but revealed strong scavenging activity against ABTS cation and nitrite. Especially, the ethylacetate fraction from the ABG extract demonstrated stronger antioxidation activity than the RG extract and other fractions. Although the antimicrobial and antithrombosis activities of the RG extract did not appear in the ABG extract, the ethylacetate fraction from the ABG extract had antibacterial activity against Staphylococcus aureus and Bacillus subtilis, and strong antithrombosis activity via the inhibition of prothrombin, blood coagulation factors and platelet aggregation. All extracts and fractions did not show any hemolytic activity against human red blood cells up to 5 mg/ml. Our results suggest that the ethylacetate fraction of ABG could be applicable to the development of functional food ingredients for antithrombosis agents.

• Journal of Life Science
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• v.33 no.1
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• pp.43-49
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• 2023

Houttuynia cordata belongs to the Saururacease family and its leaves, stems, and roots have been used as oriental medicines to treat pneumonia, acute or chronic bronchitis, enteritis, and abscesses and to remove extravasated blood. Recently, the antioxidant, anti-inflammation, antibacterial, and anti-proliferation activities and protection abilities of H. cordata against liver and neuron cell damage have been reported. In this study, ethanol extract and its solvent fractions (fractions of hexane, ethyl acetate, butanol, and water residue) were prepared, and their antithrombosis, antidiabetes, antioxidant, and hemolysis activities were evaluated. The ethyl-acetate fraction of H. cordata (EF-HC) showed the highest polyphenol and flavonoids contents among the fractions and exhibited strong antithrombosis and antioxidant activities. The EF-HC at 5 mg/ml showed 2.09-folds of thrombin time, 2.19-folds of prothrombin time, and 1.69-folds of activated partial thromboplastin time compared to the their solvent control and 30.9, 19.9, and 49.6 ㎍/ml of RC₅₀ against DPPH, ABTS, and nitrite radicals, respectively. Furthermore, the EF-HC did not show any hemolytic activity up to 1 mg/ml, whereas the hexane fraction of H. cordata showed 55% hemolysis at 1 mg/ml. This is the first report of the antithrombosis activity of H. cordata. Our results suggest that quercitirin, hyperoside, orientin, and isoquercitrin in EF-HC are related to its antithrombosis and antioxidant activities and that the EF-HC could be developed as a promising antithrombosis agent.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2001.04a
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• pp.748-751
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• 2001

Monoleaflet polymer artificial heart valve was known to show remarkable improvement in antithrombosis and pressure drop compared with other type of artificial valve. In this investigation monoleaflet the vertical and horizontal deflection pattern of polymer heart valve with three types of supporting members straight member, and two curved members was analysed using the large deformation nonlinear finite element method.

• Journal of the Korean Society for Precision Engineering
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• v.20 no.3
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• pp.120-124
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• 2003

Monoleaflet polymer artificial heart valve was known to show remarkable improvement in antithrombosis and pressure drop compared with other type of artificial valve. In this investigation of monoleaflet heart valve the vertical and horizontal deflection pattern of polymer heart valve with three types of supporting members, straight member and two curved members were analysed using the large deformation nonlinear finite element method.

• Natural Product Sciences
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• v.10 no.3
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• pp.109-113
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• 2004

The possibility of Chungpesagantang, which has been recommended for stroke patients in Korean Sasang Constitutional Clinics, and its ingredients as a novel antithrombotic agent was evaluated. Chungpesagantang potently inhibited ADP and collagen-induced rat platelet aggregation in vitro as well as ex vivo in a dose-dependent manner. Puerariae Radix and Rhei Rhizoma potently inhibited ADP-induced rat platelet aggregation ex vivo. However, Puerariae Radix did not inhibit both in vitro ADP and collagen-induced rat platelet aggregations. Chungpesagnatang and its ingredients except Rhei Rhizoma did not affect certain plasma clotting times, such as APTT, PT, and TT. Chungpesagantang and its ingredients Raphani semen and Scutellariae Radix showed significant protection against death due to pulmonary thrombosis in mice.

• YAKHAK HOEJI
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• v.53 no.5
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• pp.298-302
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• 2009

Mume Fructus extract is used in folk medicine in Eastern Asian countries including Korea. However, its therapeutic effect on thrombosis is not known. Mume Fructus methanol extract (ME) dose-dependently inhibited ADP-induced platelet aggregation and also exhibited about 130% fibrinolytic activity compared to the natto. Oral administration of ME to mice significantly extended tail-bleeding time. ME prolonged both aPTT and PT in human citrated plasma also. These results suggest that the methanol extract from Mume Fructus have antithrombosis activity.

• Biomolecules & Therapeutics
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• v.9 no.4
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• pp.258-262
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• 2001

In the advanced age, cardiovascular disease is more serious than any other disease. Especially, the thrombus causes the serious disease like apoplexia, carebri and myocardial infarction. Thrombosis is caused by the injury of endothelium and the alterations in normal blood flow. To investigate activities of Carthamus tinctorius L. Semen butanol fraction for blood coagulation system, endotoxin (4000EU/kg) was injected (i..v) to rats at 1hr after administration of Carthamus tinctorius L. Semen butanole fraction (500 mg/kg, p.o.). Carthamus tinctorius L. Semen butanol fraction was found to have antiplatelet activity in vitro. In vivo it showed a delay of blood clotting time, and prothrombin time, and reduction of fibrinogen and FDP It also increased SOD activity, and decreased MDA content. These results suggest that the antithrombosis effect of Carthamus tinctorius L. Semen butanol frac tion results from suppressive activity for a blood coagulation system and antioxidative activity.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.5
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• pp.593-598
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• 2005

Antithrombosis and antioxidant activity of the methanol extracts, which were prepared from 51 different brands of rice, were evaluated to investigate bioactive-substances. Among them, the extract of sample No. 2, 25, 40 and 41 showed strong thrombin inhibition activities ($401\~764\%$ inhibition at 5 mg/mL), and the extract of sample No. 5, 9, 23, 36, 44 and 48 showed strong DPPH scavenging activities ($40.8\~64.1\%$ at 0.4 mg/mL), respectively. The average contents of total flavonoid and polyphenol with 50 brands of rice were 51.89 mg/kg and 195.23 mg/kg, respectively. However, the activities were not linked with the contents of flavonoid and polyphenol. By the treatment at $100^{\circ}C$ for 10 min, the activities of the selected rices were severely decreased. Maximal remaining activities of antithrombosis and antioxidant were $208\%$ of No. 40 and $23.2\%$ of No. 48, respectively. These results support a functional superiority of rice-based livelihood, and suggest that the development of healthy food using functional ingredients of rice is possible.

• Proceedings of the Korean Society of Life Science Conference
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• 2006.09a
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• pp.83.1-83.1
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• 2006