• Journal of Applied Biological Chemistry
• /
• 제61권4호
• /
• pp.357-365
• /
• 2018

된장의 최적 섭취 기간을 판단하기 위해 원료 대두로부터 3년 동안 발효 숙성 하면서 6개월 단위로 시료를 채취하여 분석하였다. 이화학적 분석, 항산화력과 관능 검사를 주성분분석과 k-평균 군집 분석하여 종합적인 품질 특성을 평가하였다. 된장의 품질 특성은 PCA에 의해 1년 간격으로 큰 변화가 확인되었고, 군집 분석에 의해 숙성 기간에 따라 각각 cluster 1, 2, 3의 군집으로 분류되었다. 건강기능성과 관련된 항산화력으로 총 페놀함량, SOD 유사활성, DPPH 라디칼 소거 능력은 cluster 3에서 높았다. Cluster 3에서는 맛과 관련된 관능 평가 항목과 유리아미노산, 유기산 등도 높게 나타났다. 기호도 평가도 cluster 3에서 높게 나타났고 기호도에 가장 큰 영향을 준 관능 항목 요인은 감칠맛과 갈색이었다. 따라서 된장은 숙성 12개월 이후부터 섭취 가능하지만 된장의 맛과 건강 기능적 측면을 동시에 고려한다면 숙성 30개월 이후가 된장의 최적 섭취 시기라고 할 수 있다.

• Clinical and Experimental Reproductive Medicine
• /
• 제49권1호
• /
• pp.49-56
• /
• 2022

Objective: Oxidative stress and sperm DNA fragmentation (SDF) have been linked to idiopathic male infertility (IMI). Various antioxidants have been tried to improve semen parameters and fertility potential in IMI patients, but with inconsistent results. The study aimed to compare the effects of coenzyme Q10 (CoQ10) and Centrum multivitamins on semen parameters, seminal antioxidant capacity, and SDF in infertile men with idiopathic oligoasthenospermia (OA). Methods: This prospective controlled clinical study involved 130 patients with idiopathic OA and 58 fertile controls. The patients were divided randomly into two groups: the first group received CoQ10 (200 mg/day orally) and the second group received Centrum multivitamins (1 tablet/day) for 3 months. Semen parameters, CoQ10 levels, reactive oxygen species (ROS), total antioxidant capacity (TAC), catalase, SDF, and serum hormone levels (follicle-stimulating hormone, luteinizing hormone, testosterone, and prolactin) were compared at baseline and after 3 months. Results: Both CoQ10 and Centrum improved sperm concentration and motility, but the improvement was greater with Centrum therapy (p<0.05). Similarly, both therapies improved antioxidant capacity, but TAC and catalase improvement was greater (p<0.01 and p<0.001 respectively) with CoQ10, whereas ROS (p<0.01) and SDF (p<0.001) improvements were greater with Centrum administration. Centrum therapy was associated with reduced serum testosterone (p<0.05). Conclusion: In conclusion, both CoQ10 and Centrum were effective in improving semen parameters, antioxidant capacity, and SDF, but the improvement was greater with Centrum than with CoQ10. Therefore, Centrum-as a source of combined antioxidants-may provide more effective results than individual antioxidants such as CoQ10 in the treatment of infertile men with idiopathic OA.

• 한국수산과학회지
• /
• 제55권6호
• /
• pp.886-893
• /
• 2022

This study was conducted to evaluate the effects of dietary silymarin supplementation on the growth performance, feed utilization, innate immunity and antioxidant capacity of Pacific white shrimp Litopenaeus vannamei. Four experimental diets were formulated to contain 0, 0.025, 0.05 and 0.1% silymarin (designated as Con, S0025, S005 and S01, respectively). Triplicate groups of shrimp (initial body weight: 0.70 g) were fed each of the diets for 6 weeks. After the feeding trial, weight gain and specific growth rate were significantly higher in silymarin-supplemented groups compared to Con group. Dietary silymarin significantly enhanced protein efficiency ratio of S01 group and reduced feed conversion ratio of S005 and S01 groups. Phenoloxidase and anti-protease activities were significantly higher in S01 group compared to Con group. Glutathione peroxidase and catalase activities were significantly higher in silymarin-supplemented groups compared to those of Con group. The results of this study indicate that dietary silymarin could improve the growth performance, feed utilization, innate immunity and antioxidant capacity of Pacific white shrimp. The optimum level of silymarin in diet for Pacific white shrimp is suggested to be ≥0.025%.

• Animal Bioscience
• /
• 제36권5호
• /
• pp.731-739
• /
• 2023

Objective: This study was to evaluate the interaction of three different light-emitting diode (LED) light colors (white, green, and blue) and three intensities (5, 10, and 15 lx) on slaughter performance, meat quality and serum antioxidant capacity of broilers raised in three-layer cages. Methods: A total of 648 (8-days-old) male broiler chicks (Cobb-500) were randomly assigned in 3×3 factorially arranged treatments: three light colors (specifically, white, blue, and green) and three light intensities (namely, 5, 10, and 15 lx) for 35 days. Each treatment consisted of 6 replicates of 12 chicks. The test lasted for 35 days. Results: The semi-eviscerated weight percentage (SEWP) in 5 lx white was higher than that in 15 lx (p<0.01). The eviscerated weight percentage (EWP) (p<0.05) and water-loss percentage (WLP) (p<0.01) decreased in 10 lx white light than those in green light. Under blue light, the content of hypoxanthine (Hx) in muscle was lower than that under white and green light (p<0.01). The content of malondialdehyde (MDA) in 15 lx blue light was higher than that in 10 lx green light (p<0.05). Light color had an extremely significant effect on thigh muscle percentage, WLP, Hx, and crude protein content (p<0.01). Light intensity had a significant effect on SEWP (p<0.05), EWP (p<0.05), lightness (L^*) value (p<0.05), WLP (p<0.01), and the contents of superoxide dismutase (p<0.05), MDA (p<0.01), glutathione peroxidase (p<0.01). Conclusion: Using white LED light with 10 lx light intensity can significantly improve the chicken quality of caged Cobb broilers, improve the content of inosine acid in chicken breast and enhance the antioxidant capacity of the body. We suggest that the broiler farm can use 10 lx white LED light source for lighting in 8 to 42 days.

• 한국잠사곤충학회지
• /
• 제49권2호
• /
• pp.60-66
• /
• 2007

기능성 소재로서 각광받고 있는 뽕잎의 이용효율을 높이기 위해 특히 전라북도에서 보유하고 있는 뽕나무 유전자원을 이용하여 재배생태적 특성을 조사함과 동시에 품종별 및 시기별 뽕잎의 항산화능 변화 양상을 구명함으로써 항산화능이 높은 유전자원을 선발하고자 하였다. 그 결과를 요약하면 다음과 같다. 1. 종합적인 재배 생태적 특성 조사 결과 '신일뽕', '수원상 2호' 및 '일본금' 3계통은 뽕잎이 비교적 크고 수량이 많으며 병에 대한 저항성이 강해 전라북도 유전자원을 활용한 뽕 품종 선택시 우수 품종으로 활용할 가치가 큰 것으로 판단되었다. 전북 지역에서 뽕나무를 심을 경우 내동성에 약한 '구문용' 계통은 선택하지 않도록 주의해야 할 것이다. 2. 공시 계통 전체(15계통)의 뽕잎에 대한 항산화능을 품종별 및 시기별로 분석한 결과, 춘기 뽕잎의 경우 5개 엽기의 어린 뽕잎의 항산화능은 1380.1 nmol이었으며, 오디가 착색되기 직전에 채취한 뽕잎의 항산화능은 1611.8 nmol으로 다소 증가된 값을 나타냈다. 그러나 오디가 성숙함에 따라 뽕잎의 항산화능은 급격히 감소하였다. 3. 추기 뽕잎의 항산화능은 춘기 뽕잎의 항산화능에 비해 높았다. 즉 시기가 경과함에 따라 2109.8 nmol, 2,617.8 nmol, 3,311.5 nmol으로 증가하였다. 추기 1차(8월16일 채취)의 경우 '용천뽕'의 항산화능이 4026.7 nmol로 가장 높았으며, 다음으로 '불란서'가 2659.7 nmol의 항산화능을 나타냈다. 추기 2차(9월 15일 채취)에는 '갑선'과 '용천뽕'이 각각 3438.0 nmol, 3426.9 nmol로 높은 항산화능을 나타냈으며, 추기 3차(10월 17일 채취)의 경우 '용천뽕'(4439.1 nmol '덕천상'(4307.7 nmol)의 항산화능이 높았다.

• 대한약침학회지
• /
• 제10권3호
• /
• pp.53-62
• /
• 2007

Objective The objective of this study was to investigate the antioxidative effects of Puerariae Radix extract. Method Total antioxidant capacity (TAC), Total antioxidant response (TAR), Total phenolic content, Reactive oxygen species (ROS), 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activities, lipid peroxidation were examined. Result Total antioxidant status was examined by total antioxidant capacity(TAC) and total antioxidant response(TAR) against potent free radical reactions. TAC and TAR of Puerariae Radix extract at the concentration of 5 mg/ml were 2.02 and 1.50 mM Trolox equivalents, respectively. Total phenolic content of Puerariae Radix extract at the concentration of 5 mg/ml was 2.29 mM gallic acid equivalent. Concentration of Puerariae Radix extract at which DPPH radical scavenging activity was inhibited by 50% was 5.91 mg/ml as compared to 100% by pyrogallol solution as a reference. The inhibitory effect of the extract on lipid peroxidation was examined using rat liver mitochondria induced by FeSO4/ascorbic acid. Puerariae Radix extract at the concentration of 1 mg/ml slightly but significantly decreased TBARS concentration. The extract further prevented lipid peroxidation in a dose-dependent manner. The effect of Puerariae Radix extract on reactive oxygen species (ROS) generation was examined using cell-free system induced by hydrogen peroxide/FeSO4. Addition of 1 mg/ml of Puerariae Radix extract significantly reduced dichloroflurescein (DCF) fluorescence. The extract caused concentration-dependent attenuation of the increase in DCF fluorescence, indicating that the extract significantly prevented ROS generation in vitro. Thus antioxidant effects of Puerariae Radix extract seem to be due to, at least in part, the prevention from free radicals-induced oxidation, followed by inhibition of lipid peroxidation. Conclusion As a result, Puerariae Radix seems to have antioxitative effect and antioxidant compount.

• Journal of Nutrition and Health
• /
• 제39권8호
• /
• pp.801-807
• /
• 2006

To elucidate the effect of soy isoflavone supplementation on bone mineral density and antioxidant enzyme activity in 60 postmenopausal women residing in Iksan area were recruited. There were 31 participants in the treatment group and 29 in the control group. The treatment group consumed isoflavone extract capsules daily (which contained 90 mg of soy isoflavones) for 12 weeks. The study compared before and after isoflavone intake in the following areas. Physical examination, diet survey, bone mineral density (BMD) and antioxidant enzyme activity (superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and total antioxidant capacity (TA)). The average age of the treatment group was 64.6 years and that of the control group was 66.5 years. There were no significant differences between the two groups in terms of height, weight and body mass index. Both groups maintained a regular diet pattern in terms of their average daily nutrient intake. There were no significant differences between the treatment group (23.3 mg) and the control group (24.0 mg) in terms of daily isoflavone intake based on diet. Isoflavone supplementation of 12 weeks did not resulted in any significant changes in BMD or parameters of antioxidant enzyme activity, implying the necessity of more intensive intervention for a substantial change. In conclusion, this study revealed that antioxidant enzyme activity holds a significant relationship with the bone mineral density in postmenopausal women and further systematic research on dose and period of isoflavone supplementation is needed to clearify the positive effect of isoflavone on BMD and/or blood antioxidant capacity in postmenopausal women.

• 사상체질의학회지
• /
• 제16권1호
• /
• pp.130-147
• /
• 2004

To investigate the antioxidant capacity of traditional Korean herb medicines, water extracts from 42 species which has been used for the Taeum constitution of Sasang Medicine, were tested on their antioxidant activity using radical scavenging effects against ABTS. Some Of them showed strong antioxidant capacities at $50{\mu}g$ concentration. And, antioxidant capacities of 2 prescriptions extract of Sasang Medicine, Cheongsimyeonja-tang and Yeoldahanso-tang, in the different concentration ($10{\mu}g$, $50{\mu}g$, and $100{\mu}g$) were determined. At the same time, the antiperoxidation effects of these 2 prescriptions extract were determined. Lipid peroxidation in brain homogenates induced by NADPH and $ADF-Fe^{2+}$ was strongly inhibited by Cheongsimyeonja-tang in vitro. The above-mentioned 2 prescriptions are a potent antioxidant capacity and antiperoxidation activity, further investigation into the in vivo antioxidant therapeutic potential for treatment of human disorders in brain tissue.

• 한국식품과학회지
• /
• 제44권5호
• /
• pp.643-647
• /
• 2012

The antioxidant capacity of aloe vera gel (AG), aloe vera exudates (AE), and a low molecular filtrate of aloe vera gel (ALMF) prepared from aloe vera grown on Jeju Island, Korea was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS), and oxygen radical absorbance capacity (ORAC) assays, and total phenolic content (TPC), and total flavonoid content (TFC) were determined. The phenolic compounds in aloe samples were analyzed. Antioxidant capacities in oil-in-water emulsions following riboflavin photosensitization were analyzed using lipid hydroperoxide. AE had significantly higher antioxidant capacity than that of the other samples based on the DPPH, ABTS, and ORAC assays (p<0.05). Lipid hydroperoxide values of 5 mg/mL for AG, AE, and ALMF were 521.78, 272.32, and 699.89 mmol/kg oil, respectively, whereas that of samples without aloe vera was 893.07 mmol/kg oil over 48 h. AE had higher TPC and TFC values. Aloesin and aloin were found in AE, whereas those compounds were only found in trace amounts in AG and ALMF.

• Asian-Australasian Journal of Animal Sciences
• /
• 제28권3호
• /
• pp.374-381
• /
• 2015

This study aimed to evaluate the effects of vitamin E (VE), ferulic acid (FA) and their combination supplementation on meat quality and antioxidant capacities of finishing pigs. Sixty barrows were randomly allocated to four experimental diets using a $2{\times}2$ factorial arrangement with 2 VE supplemental levels (0 or 400 mg/kg) and 2 FA supplemental levels (0 or 100 mg/kg) in basal diets. After 28 days, six pigs per treatment were slaughtered. The results showed that VE supplementation increased loin eye area of pigs (p<0.05) and FA supplementation increased $pH_{45min}$ value (p<0.05). The interaction of $FA{\times}VE$ was observed in shear force of longissimus dorsi muscle (p<0.05). Moreover, supplementation with VE decreased hepatic and sarcous malondialdehyde (MDA) content, increased hepatic glutathione (GSH) content and sarcous glutathione peroxidase (GSH-Px) activity (p<0.05). Additionally, supplementation with FA increased hepatic GSH-Px activity and decreased sarcous MDA content (p<0.05). However, dietary treatment did not affect the expression of genes related to nuclear factor, erythroid 2-like 2 (NFE2L2) pathway. These results suggest that dietary FA and VE could partially improve meat quality and antioxidant capacity of finishing pigs, but not by activating NFE2L2 pathway under the normal conditions of farming.