• Archives of Pharmacal Research
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• 제28권11호
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• pp.1228-1232
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• 2005

Four compounds, including two novel macrolides, were isolated from an endophyte Streptomyces sp. Is9131 of Maytenus hookeri. Spectral data indicated that these compounds were dimeric dinactin (1), dimeric nonactin (2), cyclo-homononactic acid (3), and cyclo-nonactic acid (4). Bioassay results showed that dimeric dinactin had strong antineoplastic activity and antibacterial activity.

• 약학회지
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• 제17권2호
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• pp.115-121
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• 1973

Seven ${\beta}$-substituted-2,4-dihydroxy-5-nitropropiophenones were synthesized as potential antineoplastic agents, and subjected to the screening test of survival effect and cell decrease effect. Of the sunthesized compounds, ${\beta}$-(1-methyl-1-hydroxymlethy) ethylamino-2, 4-dihydrocy-5-nitropropiophenone and ${\beta}$(1,1-dihydorxymethyl) ethylamino-2,4-dihydroxy-5-dihyropropiophenone and ${\beta}$-(1,1-dihydroxymethyl) propylamino-2,4-dihydroxy-5-nitropropiophenone were found to be active against both Ehrlich ascites carcinoma and Sarcoma 180.

• 생약학회지
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• 제25권2호
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• pp.171-177
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• 1994

Antineoplastic activity against human gastric and colon carcinoma cell lines was measured in 100 extracts from 90 plants using MTT (3-[4,5-dimethyl thiazo-2-yl]-2, 5-diphenyl tetrazolium bromide) method. Four extracts from four plants have been reported to have antineoplastic effect. Extracts from remaining 86 plants failed to show significant cytotoxic effect at the concentration of less than $230\;{\mu}/ml$.

• 생약학회지
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• 제24권3호
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• pp.223-230
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• 1993

Antineoplastic activity against human gastric, colon and hepatocellular carcinoma cell lines were measured in 100 extracts from 80 medicinal plants using MTT (3-[4, 5-dimethyl thiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) method. Seventeen extracts from fourteen plants, all of which have previously been reported to have antineoplastic effect, had $IC_{50}$(50% inhibitory concentration) values of less than $230{\;}{\mu}g/ml$ in at least one of the three cell lines. Extracts from remaining sixty-six medicinal plants failed to show significant cytotoxic effect at the concentration of less than $230{\;}{\mu}g/ml$.

• 약학회지
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• 제41권3호
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• pp.283-293
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• 1997

Some analogs and their Mannich bases of Antineoplaston A10 (A10) were synthesized. Chemical yield for the 2-(or 3-)thienyl, benzol, and phenylpropionyl analogs were high but 1-naphthyl analog was synthesized in low yield. The Mannich bases formation of these analogs with morpholine went verywell compared to other bases. 1-Naphthyl, 4-nitrobenzoyl, and phenylpropionyl analogs of A10 showed weak in vitro activity but the other A10 analogs showed weaker or no activity at 10-1000mcg/ml. But their Mannich bases containing A10analogs showed good in vitro activity compared to simple A10 analogs.

• 한국자원식물학회지
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• 제6권1호
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• pp.13-23
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• 1993

To data, many types of compounds having antineoplastic activity have been isolated from higher plants, that is, alkalodids, terpenes, lignans, steroids and so on. Some of ther were isolated from Indonesian plants, Curcuma xanthorrhiza and Eurycoma longifolia. Bisaborane type compounds were compounds were isolated as antimeoplastic compounds againest Sarcoma 180A from C. xanthorrhiza, and quassinoids and euryrene type triterpenes from triterpenes from El longifolia. Casearines, a kind of diterpene, had been isolated as cytotxic components from Casearia sylvestris distributed in South America. RA series Cyclic hexapeptides isolated from Rubia akane and R. cordifolia also have strong antineoplastic activity against various types of tumors. Till now, 16 kinds of RA series compounds were isolated and named as RA-I~XVI. Moreover, monoglucoside of RA-V newly isolated from same plant. Many kinds of derivatives including natural RA compounds were tested for QSAR, and one of them, RA-VII was screened up as a most suitable substance as an antitumor agent. RA-VII(=RA 700) has strong cytotoxic activity against KB cells, P388 lymphocytic leukemia and MM2 mammary carcinoma cells. In some solution, three conformers of RA-VII were observed by NMR. It was discussed the relationship between conformation and activity. Total synthesis was already completed, but there is left room for improvement. Phase I clinical trials for RA-VII has been finished, then Phase II trials will be started before long.

• 한국자원식물학회지
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• 제10권4호
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• pp.346-350
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• 1997

식용 및 약용작물중에서 암세포에 대하여 생체세포독성을 유발하는 소재를 선발하기 위하여 한국인종양에서 유래한 대장암세포주(SNU-C4)와 위아세포주(SNU-1)에 대해 MTT검색법으로 50% 세포성장저해농도($IC_{50}$)를 측정한 결과는 다음과 같다. 1. 암세포에 대해 생체세포독성활성을 가지는 작물을 선발하기 위하여 17종의 작물을 대상으로 검색한 결과 보리, 옥수수, 오미자에서 세포독성효과가 $230{\mu}g/ml$이하의 농도로 유의한 효과를 나타내었다. 2. 품종간의 세포독성효과의 차이를 검색하기 위하여 세포독성효과를 나타낸 작물중에서는 보리와 옥수수를, 세포독성효과를 미약하게 나타내거나 나타내지 않은 작물중에서는 벼와 귀리를 대상으로 품종별로 검색한 결과, 세포독성의 유무는 1차 검색과 동일한 경향이 나타났으나, 벼에서는 품종에 따라 현저하게 세포독성의 차이를 나타내었으며, 특이할만한 점은 유색미인 흑도에서 다른 품종에 비하여 50% 세포성장저해농도가 유의하게 낮게 나타났다.

• 한국식품영양학회지
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• 제22권4호
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• pp.639-643
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• 2009

키토산 가수분해물의 세포 독성 및 항종양성 실험에서 키토산 가수분해물은 정상세포주인 Vero E6(Africa green monkey kidney cell)에 대한 세포 독성을 거의 나타내지 않았다. 정상세포주에 대한 키토산 가수분해물의 $IC_{50}$값은 1,107.95 ${\mu}g/m{\ell}$이었다. 키토산 가수분해물은 폐암 세포주인 A549, 방광암 세포주인 J82, 대장암 세포주인 SNU-C4, 위암 세포주인 SNU-1, 유방암 세포주인 ZR75-1 등과 같은 사람의 종양세포주에 대한 in vitro 항종양성을 나타내었다. 종양세포주에 대한 키토산 가수분해물의 $IC_{50}$값은 A549, J82, SNU-C4, SNU-1, ZR75-1 세포주의 경우에 각각 421.06 ${\mu}g/m{\ell}$, 417.99 ${\mu}g/m{\ell}$, 445.54 ${\mu}g/m{\ell}$, 380.65 ${\mu}g/m{\ell}$, and 460.49 ${\mu}g/m{\ell}$이었다.

• Journal of Ginseng Research
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• 제21권2호
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• pp.78-84
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• 1997

As a part of our ongoing effort to develop new antineoplastic immunostimulator from natural sources, bioassay-directed fractionationn of polysaccharides from Korean red ginseng was carried out by observing the proliferation of marine spleen cells and the generation of lymphoklne activated killer (LAK) cells. The acidic polysaccharide fractions proliferated spleen cells and generated LAK cells in proportion to their acidity in vitro. The LAK cell which was induced by ginseng showed tumoricidal activity against both NK celt sensitive and insensitive tumor target cells without major histocompatibility (MHC) restriction. Adherent macrophages and CD4+helper T cells were involved in the generation of the LAK cells. The acidic polysaccharide from Korean red ginseng synerglzed with recombinant IL-2 (rIff-2) at lower than 3 U/ml. The optimal doses of the acidic polysaccharide from Korean red ginseng for the proliferation of spleen cells and for the generation of LAK cells were 1 mg/ml and 100 $\mu\textrm{g}$/ml, respectively; this means that the mechanisms for the both activities may be different from each other.

• Archives of Pharmacal Research
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• 제8권4호
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• pp.253-256
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• 1985

The effect of skullcapflavon II, 5, 2'- dihydroxy-6, 7, 8, 6'-tetramethoxyflavone, on the growth of transplantable L 1210 and sarcoma 180 tumors in mice was studied. Intraperitional treatment of skullcapflavon II cased a significant (T/C = 166%) and a moderate (T/C = 122 %) prolongations of the life spans of ICR and $BDF_{1}$ mice respectively, which had been intraperitioneally inoculated with sarcoma 180 and L 1210 cells. Peritumoral injection of skullacapflavon II on the solid form of sarcoma 180 in mice inhibited the tumor growth strongly (Inhibition rate = 71%).