• 한국안광학회지
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• 제14권3호
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• pp.95-102
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• 2009

목적: 본 연구는 마늘 allyl 유도체의 안질환 세균에 대한 항균활성을 조사하고, 이를 다목적용액(MPS)에 응용함으로써 천연물유래 마늘성분을 이용하여 MPS 개발에 대한 기초자료를 얻고자 실시하였다. 방법: 녹농균(Pseudomonas aeruginosa)과 황색포도상구균(Staphyloccus aereus)에서 마늘유래 allyl 유도체인 diallyl sulfide(DAS)의 항균활성을 측정하였으며, 항균활성이 없는 MPS에 DAS를 함께 처리하여 세균의 생육억제 효과를 측정하였다. 결과: 50%의 생육저해($IC_{50}$)를 나타내는 DAS농도는 녹농균과 황색포도상구균 각각 0.25%와 0.64% 였다. MPS의 항균활성 실험에서 A사의 제품은 효과가 없었으며 B사의 제품은 두 균 모두에서 항균활성을 보였다. B사 MPS의 항균활성은 그람음성과 그람양성 세균에 대해 모두 유의한 차이를 나타내지 않았다. 따라서 DAS의 항균활성을 이용한 보존제의 개발을 위해 처리된 농도에서 항균활성을 나타내지 않은 A사의 MPS를 사용하여 A사의 MPS에 DAS를 첨가하여 항균활성을 조사한 바 DAS의 항균활성 효과가 나타났으며, 콘택트렌즈 살균제에 대한 적용가능성이 확인되었다. 결론: 마늘의 allyl 유도체는 황색포도상구균과 녹농균에 대한 항균작용을 나타내었으며, 녹농균에서 더큰 항균활성을 가지는 것으로 나타났다. 따라서 마늘성분과 같은 천연물유래 항균활성물질이 MPS의 보존제로 개발 가능성이 있음을 시사한다.

• 생약학회지
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• 제31권1호
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• pp.72-76
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• 2000

This study was carried out to evaluate cytotoxic effects of Ajuga multiflora Bunge extracts on murine leukemia tumor $(P388D_1)$ cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. The comparison of $IC_{50}$ values of Ajuga multiflora Bunge extracts in L1210 and $P388D_1$ cell lines showed that their susceptibility to these extracts decreased in the following order: Adriamycin>methanol extract>chloroform extract>ethyl acetate extract>hexane extract>water extract by the MTT assay. In order to develop an antimicrobial agent, dried Ajuga multiflora Bunge was extracted with several solvents, and then antimicrobial activity was investigated. The minimal inhibitory concentration (MIC) of the extract against microorganisms were also examined. Antimicrobial activities of amocla and ketoconazole as references were compared to those of extracts of $H_2O$, n-hexane, chloroform, ethyl acetate and methanol. The antimicrobial activity of all extracts from the sample had growth inhibition activity against gram-negative bacteria, gram-positive bacteria and fungi $(MIC>200\;{\mu}g/ml)$. These results suggest that the methanol soluble extract of Ajuga multiflora Bunge may be a valuable choice for the studies on the treatment of murine leukemia tumor cell lines.

• 한국식품과학회지
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• 제31권1호
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• pp.206-211
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• 1999

천연에서 발견되는 휘발성 물질인 isothiocyanate계(ITCs) 물질을 대상으로 Listeria monocytogenes, Bacillus subtilis, Pseudomonas fluorescens, Escherichia coli, Erwinia carotovora, Saccharomyces cerevisiae, Candida albicans, Aspergillus oryzae and Penicillium roqueforti 등 9종의 균주에 대한 증식 억제 효과를 측정하였으며 초산과 혼합하여 사용할 때 상승효과를 비교하였다. Allyl isothiocyanate (AIT), benzyl isothiocyanate (BIT) 및 ethyl isothiocyanate (EIT)가 대상 균주 모두에 $100{\sim}200\;{\mu}g/dish$ 수준의 최소증식저해농도(MIC)를 보였고 증식 저해 정도는 곰팡이>효모>Gram 음성 세균>Gram 양성 세균 순 이었다. 초산은 $50{\sim}500\;{\mu}g/dish$로 ITCs 보다 MIC가 낮았다. ITCs와 초산을 혼합하여 사용하는 경우 대부분의 실험 대상 균주에 대하여 증식 저해 상승효과가 인정되었고 AIT의 경우 AIT 단독 또는 초산 단독으로 사용할 때보다 $2{\sim}10$배 이상 효과가 상승하였으며 전체적으로 증식 억제 경향은 ITCs 단독 사용할 때와 같은 경향이었다.

• Journal of Nutrition and Health
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• 제35권8호
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• pp.880-885
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• 2002

중국에서 약용 및 음용식물로 사용되고 있는 여주 (Mo-mordica charantia L.)를 이용하여 추출, 분획한 후 항균, 항암 및 암예방 효과를 살펴보았다 여주의 MCU층을 제외한 모든 층에서 항균력을 보였으며 특히 Staphylococru aureus의 MCMEE층에서 가장 높은 항균활성을 나타내었다 실험에 사용한 각균에 대한 여주 분획물의 암세포 증식 억제 효과를 MTT assay로 실험한 결과, 3종의 인체 암세포 HepG2, HeLa 및 MCF극에서 모두 여주의 ethyleth-er 분획층인 MCMEE와 ethylacetate 분획층인 MCMEA 층에서 아주 높은 암세포 증식억제 효과를 나타내었다. HepG2세포를 이용하여 암예방 QR 유도 활성을 측정한 결과, 다른 분회층에 비해 비극성 용매층인 hexane 분획층 MCMH에서 아주 높은 QR 유도를 활성시키는 것으로 나타났다. 본 실험 결과, 여주에는 식품 보존과 항균제로서의 개발가능성이 보이며. 항발암 효과를 보기위한 암세포 증식 억제 효과는 ethylether층 MCMEE과 ethylacetate층 MCMEA층에서 월등하였고, 비극성 용매층인 hexane 분획층에는 암발생을 예방하는 quinone reductase inducer가 있는 것으로 사료되며. 나아가 단계적인 생리활성 연구의 분리 동정이 계속 이루어져야 할 것으로 사료된다.

• Journal of Periodontal and Implant Science
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• 제32권1호
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• pp.249-255
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• 2002

Zea Mays L. has been known to be effective for improving tissue health and Magnoliae cortex to have effective antibacterial and antimicrobial activity against pathogenic microbes. The purpose of this study was to examine the antimicrobial effects of Zea Mays L. and Magnoliae cortex extract mixtures on periodontal pathogens(Prevotella intermedia, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Streptococcus mutans )and to examine the effects on human gingival fibroblast cellular activity. Zea Mays L. and Magnoliae cortex extracts and their mixtures were prepared with various mixing ratios (0.5:1, 1:1, 1.5:1, 2:1). These extracts were loaded to periodontal pathogen cultured petri dish for antimicrobial test and also loaded to cultured human gingival fibroblast for cellular activity test. Each test was repeated 3 times and data were analyzed by one-way ANOVA with 95% confidence level. Mixture of these two extracts showed greater amount of inhibition area on periodontal pathogen and more improved gingival fibroblast activity as Zea Mays L. ratio reduced. So, mixture ratio 0.5:1 (Zea Mays L. : Magnoliae cortex) group showed statistical significance in antimicrobial activity and cellular activity among various mixtures(p < 0.05). In conclusion, 0.5:1 (Zea Mays L. : Magnoliae cortex) mixture possessed best gingival fibroblast cellular activity and antimicrobial activity toward periodontal pathogens.

• Journal of Microbiology and Biotechnology
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• 제22권2호
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• pp.264-269
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• 2012

Recently, we reported that the synthetic Coprisin analog peptide 9-mer dimer CopA3 (consisted of all-L amino acid sequence) was designed based on a defensin-like peptide, Coprisin isolated from Copris tripartitus. The 9-mer dimer CopA3 (L-CopA3) had antibacterial activity and induced apoptosis in human leukemia cells via a caspase-independent pathway. In this study, all of amino acid sequences of L-CopA3 were modified to all D-form amino acids (DCopA3) to develop a more effective antimicrobial peptide. We investigated whether D-CopA3 had antimicrobial activities against pathogenic microorganisms and pro-apoptotic effects in human leukemia cells (U937, Jurkat, and AML-2). The synthetic peptide D-CopA3 had antimicrobial activities against various pathogenic bacteria and yeast fungus with MIC values in the 4~64 ${\mu}M$ range. Moreover, D-CopA3 caused cell growth inhibition, and increased the chromosomal DNA fragmentation and the expression of inflammatory cytokines, TNF-${\alpha}$ and IL1-${\beta}$, transcripts in human leukemia cells. The all-D amino acid peptide DCopA3 proved as effective as the L-CopA3 reported previously. These results provide the basis for developing D-CopA3 as a new antibiotic peptide.

• 동의생리병리학회지
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• 제26권1호
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• pp.53-58
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• 2012

The aim of this study was to investigate the antimicrobial, antiperspirant and deodorant effect of herb mixtures including Schisandra chinensis(SC). This herb mixtures including Schisandra chinensis was made of Astragalus membranaceus(AM), Triticum aestivum(TA), Atractylodes japonica(AJ) and peppermint essential oil extracted from Mentha piperita(MP). We examined antimicrobial activity, antiperspirant activity and L-leucine dehydrogenase activity of the herbal extracts. We found that the mixture of SC and MP have the best antimicrobial effects on B. subtilis subsp. spizizenii and mixture of SC, AJ, AM and TA have best antiperspirant effect. Moreover, the combination of SC and AM showed the most inhibitory effect on L-leucine dehydrogenase activity. These results suggested that the herb mixtures Including Schisandra chinensis have good antimicrobial and antiperspirant effects.

• 한국식품위생안전성학회지
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• 제11권3호
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• pp.209-214
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• 1996

Irradiated and non-irradiated Korean medicinal herbs were extracted by water and 70% ethanol. Antimicrobial activity of these extracts were invesigated against selected food hygiene microoganisms. The ethanol extracts of the non-irradiated Agrimonia pilosa ledebour japonica Nakai, Curcuma longa Linne and Angelica gigas Nakai were completely inhibited on four species of bacteria, such as Vibrio parahaemolyticus, Clostridium perfringenes, Bacillus subtilis and Staphylococcus aureus. Also, above four strains did not have antibacterial activity in the water-ethanol mixtures. Futhermore, the ehtanol extracts of the non-irradiated Agimonia pilosa ledebour Japonica Nakai, Curcuma Iedoaria Roscoe, Curcuma longa Linne and Scutellaria baikalensis George were shown inhibitory effects against Aspergillus flavus and Pen icillium islandicum. And the water extract of Scutellaria baikalensis George was the same effect to these molds. Essentially the same results were observed when samples irradiated at a dose of 10 kGy.

• 한국임상수의학회지
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• 제9권1호
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• pp.277-281
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• 1992

To investigate the effect of chlorhexidine gluconate and povidone iodine on wound healing, their antimicrobial activity and their effects on wound contraction in rabbits were studied. Staphyococcus aureus (1${\times}$10$\^$7/) was inoculated on the full thickness skin defects (2${\times}$2cm) on the back. Antiseptics were applied on the defects 2 hours after Inoculation. The defects were swabbed for bacterial culture 24 and 48 hours after the application of antiseptics. Percentage of wound contraction, based on wound area measured on the day of surgery was calculated for each wound on days 1,7 and 14. The 0.5% chlorhexidine gluconate solution had more effective antimicrobial activity against Staphyococcus aureus in wounds than 0.05% chlorhexidine gluconate solution (p<0.05), and povidone iodine solution and saline (p<0.01). Percentages of wound contraction were not significantly different among different dilutions of the antiseptics and saline. Present study indicated that the antimicrobial activity of chlorhexidine gluconate in wound was superior to that of povidone iodine.

• 한국미생물·생명공학회지
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• 제22권2호
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• pp.217-221
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• 1994

The essential oil of astringent persimmon leaves was extracted by a gas co-distillation method and its effects on Kimchi fermentation were examined. The changes of pH and titratable acidity of Kimchi during fermentation at 15$\circ $C generally decreased by the addition of essential oil of astringent persimmon leaves, and the growth of microorganisms associated with Kimchi fermentation was also inhibited. The fermentation time of Kimchi reaching to the maximum number of cell counts was greatly extended as compared to that of the Kimchi fermented without the addition of essential oil. The greatest antimicrobial activity activity of essential oil of persimmon leaves was shown against Lactobacillus plantarum among varoius bacteria associated with Kimchi fermentation. The antimicrobial activity of essential oil against Saccharomyces cerevisiae was much higher then that against lactic acid bacteria such as L. plantarum.