Journal of Korean Ophthalmic Optics Society (한국안광학회지)

The Korean Ophthalmic Optics Society (한국안광학회)
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Antimicrobial Activity of Allyl Derivate in Multi-purpose Solution as Natural Preservative

다목적용액에서 천연보존제로서의 Allyl 유도체의 항균활성

  • Lee, Ji-Young (Department of Ophthalmic Optics, Daegu Polytechnic College University) ;
  • Suh, Seung-Kyo (Department of Pet Management, Daegu Polytechnic College University) ;
  • Sung, Duk-Yong (Department of Ophthalmic Optics, Daegu Polytechnic College University) ;
  • Youk, Do-Jin (Department of Ophthalmic Optics, Daegu Polytechnic College University) ;
  • Lee, Koon-Ja (Department of Optometry, Eulji University) ;
  • Chung, Jae-Yong (Department of Pet Management, Daegu Polytechnic College University)
  • 이지영 (대구산업정보대학 안경광학과) ;
  • 서승교 (대구산업정보대학 애완동물관리과) ;
  • 성덕용 (대구산업정보대학 안경광학과) ;
  • 육도진 (대구산업정보대학 안경광학과) ;
  • 이군자 (을지대학교 안경광학과) ;
  • 정재용 (대구산업정보대학 애완동물관리과)
  • Received : 2009.07.25
  • Accepted : 2009.09.08
  • Published : 2009.09.30
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Abstract

Purpose: The purpose of this study is to investigate antimicrobial activity of ally derivative of garlic against eye diseases, and to get basic data for an application development of the derivative, as a natural preservative, to multi-purpose solution (MPS). Methods: Antimicrobial activity of allyl derivative of garlic, diallyl sulfide (DAS), against Pseudomonas aeruginosa (P. aeruginosa) and Staphyloccus aereus (S. aereus) was determined. After DAS treatment in MPS of no microbial activity, its growth inhibition effect to bacteria was also determined. Results: DAS concentrations of $IC_{50}$ against P. aeruginosa and S. aereus were found when both bacteria were treated with DAS concentration of 0.25% and 0.64%, respectively. At MPS test, a product from Company A did not show any effects against both bacteria, but one from Company B showed antimicrobial activity against both bacteria. Antimicrobial activity test of the product from Company B in MPS did not showed any significant difference on both gram-negative and gram-positive bacteria. For a trial application of antimicrobial activity of DAS as a natural preservative, DAS was added to MPS of Company A which did not show any antimicrobial activity at treated concentration. Addition of DAS into MPS of Company A resulted in antimicrobial activity of DAS, which suggested a possible application of DAS as a germicide for contact lenses. Conclusions: This study showed allyl derivative of garlic has antimicrobial activity against both P. aeruginosa and against S. aereus, but more effects on P. aeruginosa. The result suggests that allyl derivative derived from garlic can be developed to a preservative of MPS as a natural substance.

목적: 본 연구는 마늘 allyl 유도체의 안질환 세균에 대한 항균활성을 조사하고, 이를 다목적용액(MPS)에 응용함으로써 천연물유래 마늘성분을 이용하여 MPS 개발에 대한 기초자료를 얻고자 실시하였다. 방법: 녹농균(Pseudomonas aeruginosa)과 황색포도상구균(Staphyloccus aereus)에서 마늘유래 allyl 유도체인 diallyl sulfide(DAS)의 항균활성을 측정하였으며, 항균활성이 없는 MPS에 DAS를 함께 처리하여 세균의 생육억제 효과를 측정하였다. 결과: 50%의 생육저해($IC_{50}$)를 나타내는 DAS농도는 녹농균과 황색포도상구균 각각 0.25%와 0.64% 였다. MPS의 항균활성 실험에서 A사의 제품은 효과가 없었으며 B사의 제품은 두 균 모두에서 항균활성을 보였다. B사 MPS의 항균활성은 그람음성과 그람양성 세균에 대해 모두 유의한 차이를 나타내지 않았다. 따라서 DAS의 항균활성을 이용한 보존제의 개발을 위해 처리된 농도에서 항균활성을 나타내지 않은 A사의 MPS를 사용하여 A사의 MPS에 DAS를 첨가하여 항균활성을 조사한 바 DAS의 항균활성 효과가 나타났으며, 콘택트렌즈 살균제에 대한 적용가능성이 확인되었다. 결론: 마늘의 allyl 유도체는 황색포도상구균과 녹농균에 대한 항균작용을 나타내었으며, 녹농균에서 더큰 항균활성을 가지는 것으로 나타났다. 따라서 마늘성분과 같은 천연물유래 항균활성물질이 MPS의 보존제로 개발 가능성이 있음을 시사한다.

Keywords

References

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