• Journal of Applied Biological Chemistry
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• 제51권3호
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• pp.111-116
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• 2008

Aralia elata seeds were successively extracted with water, methanol, ethanol, acetone and chloroform. The crude extracts were investigated for antioxidant and antidiabetic activities. The antioxidant properties of various extracts were evaluated by antioxidant tests, such as DPPH free radical-scavenging activity, hydroxyl radical-scavenging assay, metal-chelating activity, lipid peroxidation inhibition activity and reducing power assay. The 70% methanol extract exhibited the highest activity in the in vitro models of DPPH free radical-scavenging activity, metal-chelating activity, and reducing power assay. Acetone extract showed good effects on lipid peroxidation inhibition and hydroxyl radical-scavenging assay at a low concentration. In addition, the $\alpha$-glucosidase inhibition assay showed that 70% methanol extract had the highest activity. These results indicate the high possibility of using A. elata seeds for medical application due to their efficient antioxidant properties.

• The Korean Journal of Physiology and Pharmacology
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• 제5권2호
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• pp.183-188
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• 2001

To elucidate antidiabetic effect and mechanism(s) of acarbose in a polygenic spontaneous hyperglycemic and hyperinsulinemic diabetic animal model, $KKA^y$ mice, acarbose was administered orally for 4 weeks and effects on body weight, plasma glucose and insulin levels, genetic expressions of intestinal sucrase-isomaltase (SI), sodium-glucose cotransporter (sGLT1) and glucose transporter in quadriceps muscle (GLUT4) were examined in this study. Although no differences in body weight were detected between control and acarbose-treated groups, plasma glucose level in acarbose-treated group was markedly reduced as compared to the control. In the mechanism study, acarbose downregulated the SI and SGLT1 gene expressions, and upregulated the GLUT4 mRNA and protein expressions when compared to the control group. In conclusion, the data obtained strongly implicate that acarbose can prevent the hyperglycemia in $KKA^y$ mice possibly through blocking intestinal glucose absorption by downregulations of SI and sGLT1 mRNA expressions, and upregulation of skeletal muscle GLUT4 mRNA and protein expressions.

• Natural Product Sciences
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• 제5권4호
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• pp.155-158
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• 1999

Several biological activities of extracts from roots of Cichorium intybus Linne (Compositae) were studied in this paper. The antiinflammatory activity of the methanol extract of this root was investigated against carrageenin induced edema in rat‘s hind paw. Significant inhibitory effects were observed at the dose of 1,000 mg/kg and were compared with aspirin as a control. The hepatoprotective activities of the methanol extract, ethylacetate and butanol fraction were studied on mice whose livers are damaged by $CCl_4$. The serum transaminase activities (ALT, AST) were reduced at the dose of 1,000 mg/kg of the methanol extract, 500 mg/kg of ethylacetate and butanol fraction, respectively. The bile juice secretion was also increased significantly from each fraction. The antidiabetic activity was examined on strepto-zotocin-induced diabetic rats with methanol extract. Methanol extract gave a significant reduction of blood glucose levels in 1 week and 3 weeks.

• Journal of Ginseng Research
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• 제21권3호
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• pp.169-173
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• 1997

The effects of extract mixture of red ginseng and steamed Rehmaniae radix prepared for antidiabetic activity was examined in streptozotocin-Induced diabetic rats. The increased blood glucosec level in the streptozotocin-induced diabetic rats was significantly decreased by the treatment with the mixture (800, 1600 mg/kg, p.o.). However, neither red ginseng extract nor steamed Rehmaniae radix extract alone showed significant hypoglycemic effects. The mixture prevented a weight loss in streptozotocin-induced diabetic rats. These results suggest that the mixture has the relieving action against streptozotocin-induced hyperglycemia.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.412-413
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• 2010

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.119.2-119.2
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• 2002

• Toxicological Research
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• 제25권2호
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• pp.93-99
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• 2009

This study investigated the effect of Corni Fructus (Cornus officinalis Sieb. et Zucc.) extract on blood glucose and insulin resistance in db/db mice. Seven weeks old male mice were divided into normal control group (NC), diabetic control group (DC) and Corni Fructus treated diabetic group (DCF). Over an 8-week experimental period, Corni Fructus extract was administered orally at 500 mg/kg BW/day. Corni Fructus inhibited increase in blood glucose level during the OGTT (oral glucose tolerance test). At 8 weeks after beginning of the experiment, blood glucose level in the DCF group was significantly lower (p<0.01) than the DC group. Final fasting serum glucose and triglyceride in the DCF group were significantly lower (p<0.05) than the DC group by 32% and 41% respectively. Serum insulin did not differ among the NC, DC and DCF groups. The mRNA expression of adiponectin, GLUT 4 and PPAR-$\gamma$ in adipose tissue in the DC group were significantly lower than the NC group and they were higher in the DCF group than the DC group by 76%, 130% (p<0.05) and 43%, respectively. In conclusion, these results indicated that Corni Fructus would have antidiabetic effects via improving insulin resistance in favor of higher glucose utilization and reducing blood glucose level in db/db mice.

• The Korean Journal of Physiology and Pharmacology
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• 제26권2호
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• pp.135-144
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• 2022

An antidiabetic drug, rosiglitazone is a member of the drug class of thiazolidinedione. Although restrictions on use due to the possibility of heart toxicity have been removed, it is still a drug that is concerned about side effects on the heart. We here examined, using Chinese hamster ovary cells, the action of rosiglitazone on Kv1.5 channels, which is a major determinant of the duration of cardiac action potential. Rosiglitazone rapidly and reversibly inhibited Kv1.5 currents in a concentrationdependent manner (IC₅₀ = 18.9 μM) and accelerated the decay of Kv1.5 currents without modifying the activation kinetics. In addition, the deactivation of Kv1.5 current, assayed with tail current, was slowed by the drug. All of the results as well as the usedependence of the rosiglitazone-mediated blockade indicate that rosiglitazone acts on Kv1.5 channels as an open channel blocker. This study suggests that the cardiac side effects of rosiglitazone might be mediated in part by suppression of Kv1.5 channels, and therefore, raises a concern of using the drug for diabetic therapeutics.

• Journal of Ginseng Research
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• 제27권2호
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• pp.56-61
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• 2003

복강 내당능 시험과 MLD STZ 당뇨를 유발시킨 rat에서 백삼, 홍삼, 화기삼의 항당뇨 활성을 비교한 결과를 요약하면 다음과 같다. 1. 복강 내당능 시험에서 백삼은 효과를 나타내지 못한 반면 홍삼과 화기삼은 대조군에 대하여 유의적인 변차를 나타내었다. MLD STZ로 당뇨를 유발시킨 SD계 흰쥐에 백삼, 홍삼, 화기삼을 2주간 투여 후 혈당강하 활성을 비교한 결과 홍삼 투여군의 혈당이 가장 낮았으며 백삼과 화기삼은 비슷한 크기의 활성을 나타내었다. 2. 백삼과 홍삼, 화기삼은 모두 고혈당에 의한 체중 감소 현상을 개선시켰으며 각 투여군 간에는 유의적인 차이는 없었다. 3. 당뇨의 대표적인 증상인 다식 (多食)과 다음 (多飮)현상이 백삼, 홍삼, 화기삼 투여에 의하여 개선되었으며 그 중 홍삼에 의한 효과가 가장 컸다. 4. 혈당강하 활성과 ginsenoside profile간의 상관관계를 살펴본 결과 PPT/PPD ratio가 가장 큰 홍삼의 혈당강하 활성이 백삼과 화기삼에 비해 높은 것으로 보아 PPT/PPD ratio가 혈당강하 활성의 key ratio가 아닌가 추측된다.

• 생약학회지
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• 제46권4호
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• pp.321-326
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• 2015

The prevalence of diabetes mellitus continues to rise alarmingly because of industrialization of society. The aim of this study was to investigate the antidiabetic effects of black ginseng extract (BGE) which was performed panax ginseng. First we examined the inhibitory effects of BGE on ${\alpha}$-glucosidase. But there was no practical effects in our observations. We, also, investigate the effects of BGE on glucose uptake of skeletal muscle using 2-NBDG in $C_2C_{12}$ myotube. BGE significantly improved the glucose uptake considered as a lowered blood glucose level. Effects of GBG05-FF on fasting blood glucose and glycated hemoglobin (HbA1c) were investigated in streptozotocin (STZ)-induced diabetic mouse. After injection of STZ, fasting blood glucose and glycated hemoglobin rapidly increased. But STZ-induced diabetic mouse treated with GBG05-FF significantly reduced the level of fasting blood glucose and HbA1c. This results showed that supplementation of BGE improve the diabetic parameters and BGE have a potentiality as a functional food for Diabetes mellitus.