• 치위생과학회지
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• 제23권4호
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• pp.389-395
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• 2023

Background: Dental caries prevention is a key research focus in dentistry, requiring advancements in the formulation of dental sealants. This study investigated the physical and antibacterial attributes of dental sealant enriched with phytoncide. Methods: Phytoncide was mixed with a commercially available dental sealant (Clinpro) at concentrations of 0 (control), 1.5%, 3%, and 4.5% by weight (wt%). The flexural strength, curing depth, and wettability of the dental sealant were measured. Antibacterial properties against Streptococcus mutans were evaluated through the enumeration of colony-forming units. Statistical analyses employed one-way variance analysis followed by Tukey's test (p<0.05). Results: The dental sealant containing 3% phytoncide showed no significant difference in flexural strength and curing depth compared with that in the control group (p>0.05). The flexural strength and curing depth decreased with increasing phytoncide content and significantly differed in sealant containing 4.5 wt% phytoncide (p<0.05). Wettability did not differ between the experimental and control groups (p>0.05). The antibacterial properties of the sealant containing 1.5% phytoncide were the same as those of the control group (p>0.05). The bacterial viability was significantly reduced in groups containing 3% and 4.5% phytoncide compared with that in the control group (p<0.05). Conclusion: Dental sealants incorporating phytoncide have a promising potential as antibacterial dental materials.

• 한국산학기술학회논문지
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• 제22권1호
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• pp.562-567
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• 2021

다양한 건축시설 및 생활제품에 적용하기 위한 기능성 항균강화유리개발을 위해 국산 소재들의 항균력을 조사하여, 특정농도에서 99% 이상의 항균력을 갖는 은, 구리, 아연으로 강화유리 시편을 제작하였다. 은, 구리, 아연을 Ethylene glycol + Glycerol로 분산시킨 시편의 항균력은 모두 99% 이상으로 측정되었고, Ethylene glycol과 Glycerol을 단독으로 사용했을 때는 항균력이 감소되었다. 표면분석기를 사용하여 세척에 의한 결합된 금속소재의 두께 변화를 측정한 결과, 물을 포함한 다양한 종류의 세제로 세척한 시편에서는 표면에 도포된 소재의 두께가 1% 미만으로 감소하였지만, 염기성 세제에서는 약 10%의 표면두께 감소가 확인되었다. 또한, 시편의 인체 안전성을 확인하기 위해, MTT 분석법을 통한 세포독성실험을 수행하였고, 항균물질들이 도포된 시편에서의 세포독성은 대조군과 비교하였을 때 거의 나타나지 않았다. 마지막으로, 시제품의 항균력이 99% 이상임을 Bacterial Live/dead kit을 이용하여 확인하였고, 공인인증기관의 필름밀착법으로 다시 한 번 시제품의 항균력을 검증하였다. 결국, 본 연구에서는 국산소재들의 항균력을 검증과 함께 이를 이용한 고기능성 항균효과 갖는 강화유리를 개발하였다.

• Toxicological Research
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• 제21권1호
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• pp.39-43
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• 2005

The present study was carried out to investigate the antibacterial activity against Staphylococcus aureus with antibiotic-resistance in vitro and the skin irritation in rabbits with surfactin produced by Bacillus subtilus Complex BC1212. The antibacterial activities of selected antimicrobial agents (surfactin, amoxacillin, colistin, norfloxacin and streptomycin) were evaluated by using the broth microdilution method. As the results, the minimum inhibitory concentration (MIC) of the surfactin was less than 15.6 ㎍/ml. In the skin irritation test, two out of 4 rabbits showed very slight edema at 24 h after the administration of surfactin, and then recovered at 72 h. The change of body weight was normal during the skin irritation test. The primary irritation index in accordance with the Draize evaluation of topical reaction was calculated to be '0.125', which meant not irritating. Based on these results, it could be concluded that the test agent, surfactin, was a non-irritant. We could also think that the surfactin may be useful for the treatment of S. aureus infections such as bovine mastitis.

• Advances in Traditional Medicine
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• 제8권3호
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• pp.316-321
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• 2008

A total of 33 extracts representing 26 plant species belonging to 24 families were collected from different regions of Bangladesh, and screened for their in vitro antibacterial activity against several pathogenic Gram-positive and Gram-negative bacterial strains using the conventional disc diffusion method. The most potent activity was exhibited by the extracts of Aegiceras corniculatum, Alocasia fornicata, Ceriops decandra, Cuscuta reflexa, Lasia spinosa, Lantana camara, Pandanus foetidus and Xylocarpus granatum. The extracts of Abtilon indicum, Derris trifoliata, Dendrophthoe falcat, Ruellia tuberosa and X. moluccensis did not show any antibacterial properties at test concentrations.

• Advances in Traditional Medicine
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• 제8권4호
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• pp.323-328
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• 2008

The MeOH extract of seeds of Cerbera odollam Gaertn. (Apocynaceae) was screened for its antibacterial, cytotoxic and neuropharmacological activities. The extract showed moderate anti-bacterial activity against Salmonella typhi, Streptococcus saprophyticus, and Streptococcus pyogenes. It exhibited high level cytotoxicity against brine shrimp ($LC_{50}$: $3\;{\mu}g/ml$). The extract potentiated pentobarbital induced sleeping time in mice which was further supported by the exploratory behavior test at dose of 25 mg/kg. The overall results tend to suggest the antibacterial, cytotoxic and CNS depressant activities of the extract.

• Applied Biological Chemistry
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• 제38권3호
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• pp.269-272
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• 1995

산국과 감국의 용매분획물과 sesquiterpenoid lactones, Compound I, II를 4종의 미생물에 대하여 항균실험을 하였다. 이 중에서 산국의 chloroform분획과 sesquiterpenoid lactones, Compound I에서 B. subtilis과 V. parahaemolyticus에 대하여 항균력이 있었다. 그리고 야생감국의 chloroform분획과 sesquiterpenoid lactones 에서는 항균력이 산국보다 약하게 나타났으며, Compound II와 재배감국의 모든 분획은 미생물에 대하여 항균력이 전혀 나타나지 않았다.

• 한국염색가공학회지
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• 제12권5호
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• pp.315-322
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• 2000

The purpose of this study was to investigate the antibacterial effects of natural colors extracted from yellow natural dyes(Tumeric, Amur Cork Tree and Onion Shell). The water and the methanol extracts of Tumeric and Amur Cork Tree significally decreased the growth of E. coli in vitro and the methanol extract of Tumeric exhibited the strongest inhibitory effect among the samples. Silk and nylon fabrics dyed with water and methanol extracts of the yellow natural dyes showed antimicrobial activities against E. coli and Staph aureus in the Bioassay Test. Nylon fabric dyed with methanol extracts of them showed strong antibacterial effect on E. coli compared with that of water extracts. However, slik fabrics dyed with the extracts could not reduce the growth of E. coli. Silk or nylon fabrics dyed with methanol or water extracts of yellow natural dyes showed antimicrobial activities against Staph aureus. The antimicrobial activity of the fabrics dyed with methanol extracts from Tumeric, Amur Cork Tree and Onion Shell was stronger than that of water extracts, especially, the fabrics dyed with Tumeric extract showed the highest antibacterial property among the dyed fabrics.

• International Journal of Oral Biology
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• 제46권1호
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• pp.45-50
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• 2021

This study evaluated the antimicrobial activity of Endoseal TCS, an mineral trioxide aggregate-based root canal sealer, mixed with water-soluble mangostin derivatives (WsMD) of Garcinia mangostana L. (mangosteen) ethanol extract against Enterococcus faecalis and Staphylococcus aureus. The antibacterial activity of Endoseal TCS mixed with WsMD against three strains of E. faecalis and three strains of S. aureus was performed using agar diffusion test. The data showed that Endoseal TCS mixed with 0.115% WsMD had a zone of inhibition of 0.7 ± 0.2-2.4 ± 0.1 mm. The results suggest that Endoseal TCS mixed with WsMD of Garcinia mangostana L. ethanol extract is useful as a root canal sealer with antibacterial activity against E. faecalis and S. aureus.

• 한국산학기술학회논문지
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• 제11권3호
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• pp.1139-1145
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• 2010

본 연구는 아유르베다의 모발과 두피 프로그램을 적용하고 그 효과를 분석하고자 한다. 아유르베딕(Ayurvedic) 처치는 자연치유력에 의해 면역력을 강화시킨다. 아유르베딕 베이스 오일과 에센셜 오일을 모발과 두피에 사용했을 때, 두피에 상주하는 균에 대한 항균효과를 알아보기 위해 MIC test 와 Halo test를 통해 측정하였다. 그 결과는 다음과 같다. 9종류의 베이스 오일과 18종류의 에센셜 오일을 이용하여 Bacillus subtilis, Propionibacterium acnes균에 대한 베이스 오일과 에센셜 오일의 항균효과를 MIC test를 통해 조사하였다. 실험 결과, 베이스 오일에서는 모두 균에 대한 항균효과가 없는 것으로 나타났으며, 에센셜 오일은 Chamomile, Clary sage, Jasmine, Neroli, Rose오일에서 두 균에 대해 모두 5% 이하에서 항균효과가 없는 것으로 나타났다. 두 균주에 대해 Thyme, Geranium, Lavender, Tea tree가 모두 효과가 높은 것으로 조사되었다. Geranium, Lavender, Thyme 에센셜 오일과 Sesame and Coconut 베이스 오일을 이용하여 7가지 공시균주에 대한 Halo test결과 베이스 오일에서는 항균효과가 없는 것으로 나타났다. 에센셜 오일은 7가지 공시균주에 대해 모두 항균성이 있는 것으로 조사되었다. Thyme은 MIC test 와 Halo test 결과 항균력이 가장 높은 것으로 나타났다.

• 한방재활의학과학회지
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• 제25권2호
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• pp.1-13
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• 2015

Objectives Methicillin-Resistant Staphylococcus aureus (MRSA) is a human pathogen and a major cause of hospital-acquired infections. New antibacterial agents that have not been compromised by bacterial resistance are needed to treat MRSA-related infections. In this study, we investigated the antimicrobial activity ofethanol extract of Haedokgeumhwa-san (HGH) which prescription is composed of korean medicine against MRSA. Methods The antibacterial activity of HGH extract was evaluated against MRSA strains by using the Disc diffusion method, broth microdilution method (minimal inhibitory concentration; MIC), checkerboard dilution test, and time-kill test; its mechanism of action was investigated by bacteriolysis, detergent or ATPase inhibitors. The checkerboard dilution test was used to examined synergistic effect of ampicillin, oxacillin, ciprofloxacin, vancomycin, gentamicin and norfloxacin in combination with HGH ethanol extract. A time-kill assay was performed a survival curve which was obtained by plotting viable colony counts depending on time on bacterial growth. Results The minimum inhibitory concentration (MIC) of ethanol extract (HGH) ranged from 1,000 to $2,000{\mu}g/mL$ against all the tested bacterial strains, respectively. We are able to confirm that HGH extract has potentially strong antibacterial activity. In the checkerboard dilution test, fractional inhibitory concentration index of HGH in combination with antibiotics indicated synergy or partial synergism against S. aureus. A time-kill study showed that the growth of the tested bacteria was considerably inhibited after 8 hr of treatment with the combination of HGH with selected antibiotics. For measurement of cell membrane permeability, HGH $250{\sim}1,000{\mu}g/mL$ along with concentration of Triton X-100 (TX) and Tris-(hydroxymethyl) aminomethane (Tris) were used. In the other hand, N,N-dicyclohexylcarbodimide (DCCD) and Sodium azide ($NaN_3$) was used as an inhibitor of ATPase. TX, Tris, DCCD and $NaN_3$ cooperation against S. aureus showed synergistic action. Accordingly, antimicrobial activity of HGH was affected by cell membrane and inhibitor of ATPase. Conclusions These results suggest that Haedokgeumhwa-san extract has antibacterial activity, and that HGH extract offers a potential as a natural antibiotic against MRSA.