• Journal of Microbiology and Biotechnology
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• 제23권10호
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• pp.1357-1364
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• 2013

Human skin is continuously exposed to ultraviolet (UV)-induced photoaging. UVA increases the activity of MMP-1 in dermal fibroblasts through mitogen-activated protein kinase (MAPK), p38, signaling. The irradiation of keratinocytes by UVA results in the secretion of the inflammatory cytokine, tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), and the stimulation of MMP-1 in normal human dermal fibroblasts (NHDFs). Lipoteichoic acid (LTA) is a component of the cell wall of gram-positive Lactobacillus spp. of bacteria. LTA is well known as an anti-inflammation molecule. LTA of the bacterium Lactobacillus plantarum has an anti-photoaging effect, but the potential anti-photoaging effect of the other bacteria has not been examined to date. The current study showed that L. sakei LTA (sLTA) has an immune modulating effect in human monocyte cells. Our object was whether inhibitory effects of sLTA on MMP-1 are caused from reducing the MAPK signal in NHDFs. It inhibits MMP-1 and MAPK signaling induced by UVA in NHDFs. We also confirmed effects of sLTA suppressing TNF-${\alpha}$ inducing MMP-1 in NHDFs.

• 대한예방한의학회지
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• 제19권1호
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• pp.109-116
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• 2015

Objective : Solar ultraviolet (UV) light can cause photoaging of human skin. Many researchers have focused on effective prevention to mitigate the aging process. This study was aimed to investigate anti-wrinkle effects of pomegranate concentration solution (PCS) using photoaged hairless mouse model. Method : To clarify the protective effect of PCS against UVB-induced photo-damage, water capacity was measured with transepidermal water loss (TEWL) in SKH-1 hairless mice. In addition, image of skin replicas was analyzed. Results : Our results showed that the PCS treatment protects skin against UVB-induced photoaging, based on the parameters including moisture capacity and TEWL. According to visual assessment of skin replica, application of 1 ml/kg PCS inhibited UV-induced wrinkle formation in mice skin as compared to the vehicle-applied control mice. Conclusion : Taken together, these results indicated that PCS could improve skin wrinkle formation induced by UVB irradiation in SHK-1 hairless mice. PCS could be applied as an anti-wrinkle agent.

• 대한화장품학회지
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• 제45권1호
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• pp.27-36
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• 2019

대두(Glycine max)는 식물성 단백질이 풍부한 식품 중 하나로, 생리활성물질을 다량 함유하고 있어 심혈관질환, 비만 등에 효능이 있을 뿐 아니라 피부 개선 효과도 있는 것으로 보고되어 있다. 본 연구에서는 대두 단백질을 유산균인 Lactobaillus Rhamnosus 균주에 의해 발효한 대두발효물의 광노화 예방 효과를 조사하기 위해 자외선으로부터 기인한 세포독성에 대한 보호 효과 및 콜라겐 생성, 티로시나아제 활성, 멜라닌 색소 생성에 대한 평가를 실시하였다. 결과로서 대두발효물 처리군은 피부섬유아세포에서 자외선으로 유도된 세포독성을 억제하여 보호 효과를 나타내었으며, 세포 내 콜라겐 합성을 증가시켜 주름 개선의 가능성을 확인하였다. 또 다른 실험에서 티로시나아제 활성 및 멜라닌 색소 생성을 억제하여 피부 개선 효과를 나타내었다. 이러한 결과로 미루어 볼 때 대두발효물이 자외선으로부터 유도되는 주름, 색소 침착 등의 피부 손상을 예방하는 새로운 기능성 소재로서의 활용 가능성을 가지는 것으로 평가된다.

• 한국식생활문화학회지
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• 제35권5호
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• pp.478-482
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• 2020

We evaluated the protective effects of cricket methanol extract (CME) on ultra-violet B (UVB)-induced photoaging in human skin fibroblasts. The fibroblast cells were treated with 10, 50, and 100 ㎍/mL of CME for 24 h, and then exposed to UVB (30 mJ/㎠). CME showed a dose-dependent cytoprotective effect without any observable cytotoxicity. CME reduced UVB-induced production of reactive oxygen species (ROS) by 34.4, 34.9, 40.6% at concentrations of 10, 50, 100 ㎍/mL respectively. CME inhibited the release of matrix metalloproteinase (MMP) 1 and 3. Furthermore, CME also reduced UVB-induced collagen degradation in the fibroblast cells. Taken together, our data suggests that CME has a significant protective effect on UVB-induced photoaging of the skin. This benefit occurs through multiple mechanisms. The results also suggest a potential role for CME as an ingredient in anti-photoaging cosmetic products in the future.

• 한국식품과학회지
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• 제52권5호
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• pp.467-475
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• 2020

자외선(Ultraviolet radiation)은 피부 주름 형성과 피부 건조를 특징으로 하는 광노화의 주요 원인이다. 모자반과(Sargassaceae)에 속하는 모자반(Sargassum fulvellum)은 항염증, 항산화, 항아토피 활성을 갖고 있다. 특히, 모자반은 인간 각질세포(keratinocyte)와 실험동물의 피부에서 UVB에 의한 염증 반응을 완화시키는 것으로 보고되었다. 그러나, 현재까지 모자반 추출물의 광노화 억제 효과에 대해서는 보고된 바가 없다. 본 연구에서는 광노화를 유도하기 위하여 UVB를 SKH-1 무모 마우스의 등 부분에 8주간 처리하였으며, 동시에 모자반 추출물을 300 mg/kg의 농도로 매일 경구 투여하였다. 현상학적으로, 모자반 추출물은 주름 형성, 홍반 및 피부 두께를 감소시켰으며, 피부 탄력을 증가시켰다. 모자반 추출물은 콜라겐 합성 유전의 발현을 증가시키고, matrix metalloproteinase의 발현을 감소시킴으로써 콜라겐의 하이드록시프롤린(hydroxyproline) 함량을 증가시켰다. 추가적으로, 모자반 추출물의 경구 투여는 무모 마우스의 피부 수분 함량을 증가시켰으며, 경피수분손실(transepidermal water loss)을 감소시켰다. 모자반 추출물은 각질세포막 형성에 관여하는 involucrin, loricrin, transglutaminase의 발현과 자연보습인자 관련 생체지표인 filaggrin 및 caspase-14의 발현을 증가시켰다. 종합적으로, 모자반 추출물은 피부의 콜라겐 손실과 건조를 완화함으로써 광노화 억제 활성을 제공하였다. 모자반 추출물은 피부 광노화를 억제하기 위한 기능성 식품 소재로서 활용될 수 있을 것으로 판단된다.

• 동의생리병리학회지
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• 제28권1호
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• pp.35-44
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• 2014

This study was performed to investigate the anti-photoaging effects of Persimmon leaf tea(PLT) in hairless mice(SKH-1) exposed to UVB radiation. The animals were divided into non-treated group (normal, N) and UV-radiated groups. UV-radiated groups were divided into only UV-radiated group(control, C) and UV-radiated and PLT treated experimental groups[first extraction treated group(PLT-I), second extraction treated groupe(PLT-II), and third extraction treated group(PLT-III)]. Three PLT treated experimental groups of mice were treated with both oral administration(300mg/Kg B.W./day) and topical application (100 ul of 2% conc./mouse/day) for 4 weeks. Anti-photoaging effects of Persimmon leaf were evaluated by MTT assay, anti oxidative reaction, MMP immunohistochemistry, gelatin zymography assay and RT-PCR observations. Treatment with Persimmon leaf tea(PLT)-I, and -III groups decreased immunohistochemical density of matrix metalloproteinases(MMP)-3 and -9 related to degradation of extracellular matrix in skin. Especially, immunohistochemical density of MMP-2 decreased in PLT-I, -II and -III groups in skin. On the effects of antioxidant function on the treatment with Persimmon leaf tea(PLT), treatment of HaCaT cells with extracts of PLT-I and PLT-II had also significantly reduced intracellular ROS produced by UVB irradiation in a dose dependent manner(PLT-I, p<0.05, p<0.01, p<0.001; PLT-II, p<0.01, p<0.001). Gelatin zymography assay revealed that PLT-II and PLT-III (200 ug/ml) had inhibitory effect on MMP-9 expression in UVB-radiated HaCaT cells. Western blot analysis revealed that PLT-1, -II and -III groups down-regulates the expression of inflammatory associated genes(IL-$1{\beta}$) and PLT-1 and -II groups down-regulates the expression of TNF-${\alpha}$ in a dose dependent manner. Our study suggests that Persimmon leaf tea(PLT) extracts participates in inhibitory effects on the morphological and molecular experiments related to photoaging skin on UVB irradiated hairless mice.

• Journal of Microbiology and Biotechnology
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• 제31권4호
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• pp.630-636
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• 2021

Glabridin, a compound of the flavonoid, has shown outstanding skin-whitening and anti-aging properties, but its water insolubility limits its wide application. Therefore, glabridin liposome (GL) has been developed to improve its poor bioavailability, while there are few studies to evaluate its amelioration of UVB- induced photoaging. This study is performed to investigate the amelioration of GL against UVB- induced cutaneous photoaging. The prepared GL has a spheroidal morphology with an average diameter of 200 nm. The GL shows lower cytotoxicity than glabridin, but it has a more effective role in inhibition of melanin. Moreover, the application of GL can effectively relieve UV radiation induced erythema and leathery skin, associated with the down-regulated expression of inflammatory cytokines (TNF-α, IL-6 and IL-10). Taken together, these results demonstrate that GL has potentials as topical therapeutic agents against UVB radiation induced skin damage through inhibiting inflammation.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2003년도 Annual Meeting of KSAP : International Symposium on Pharmaceutical and Biomedical Sciences on Obesity
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• pp.110-110
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• 2003

It is well-documented that decreased antioxidant defense system by ultraviolet(UV) irradiation is the most important reason to induce the skin aging, especially photoaging. Chlorogenic acid(CA), a nonflavonoid catecholic compound, is present in the diet as part of fruits, tea, coffee and wine and has been reported to have anti-inflammatory, antimutagenic and anticarcinogenic activities. In this study, we examined the effects of CA on the UV -induced photoaging. Firstly, we investigated the protective effect of CA on antioxidant defense system in HaCaT human keratinocytes after UV irradiation treatment. UV irradiation decreased antioxidant defence enzyme activities of superoxide dismutase, catalase and GSH contents, which were restored by CA. To elucidate the effect of CA, 1% of CA and vehicle were applied to human buttock skin before and after UV irradiation (2MED). CA prevented UV -induced matrix metalloproteinase-1 mRNA expression and procollagen mRNA depression. And CA also increased CD1a(Langerhans cell) expression significantly. Our results suggest that CA has protective effects on UV -induced photoaging by increasing cellular antioxidant defense system. Therefore, CA may be a useful anti-aging agent for cosmetic purpose.

• Journal of Microbiology and Biotechnology
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• 제28권8호
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• pp.1391-1400
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• 2018

Angelica tenuissima root has historically been used as a traditional medicine in Korea. Previous studies have identified the anti-melanogenic effects of the extract of A. tenuissima root fermented by Aspergillus oryzae (FAT). This study investigated the protective effects of FAT against ultraviolet light B exposure (UVB; $30mJ/cm^2$) in HaCaT (human keratinocyte) or Hs68 (human foreskin fibroblast) skin cells. FAT treatment was able to stimulate wound healing rate at the basal condition. FAT also favored the maintenance and/or improvement of extracellular matrix impairment caused by UVB irradiation through: 1) upregulation of procollagen Type-1 synthesis and secretion; 2) suppression of MMP-1 and elastase expression. FAT was able to play a role in the attenuation of inflammatory responses caused by UVB irradiation via upregulation of photo-protective hemeoxygease-1 and suppression of proinflammatory cyclooxygenase-2 expression. After further verification of the anti-photoaging potential of FAT, it could be utilized as an effective ingredient in anti-aging and anti-wrinkle cosmetics.

• Preventive Nutrition and Food Science
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• 제17권4호
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• pp.245-253
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• 2012

Collagen tripeptide (CTP) is a functional food material with several biological effects such as improving dry skin and wound and bone fracture healing. This study focused on the anti-photoaging effects of CTP on a hairless mouse model. To evaluate the effects of CTP on UVB-induced skin wrinkle formation in vivo, the hairless mice were exposed to UVB radiation with oral administration of CTP for 14 weeks. Compared with the untreated UVB control group, mice treated with CTP showed significantly reduced wrinkle formation, skin thickening, and transepidermal water loss (TEWL). Skin hydration and hydroxyproline were increased in the CTP-treated group. Moreover, oral administration of CTP prevented UVB-induced MMP-3 and -13 activities as well as MMP-2 and -9 expressions. Oral administration of CTP increased skin elasticity and decreased abnormal elastic fiber formation. Erythema was also decreased in the CTP-treated group. Taken together, these results strongly suggest that CTP has potential as an anti-photoaging agent.