• Bulletin of the Korean Chemical Society
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• 제34권9호
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• pp.2629-2634
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• 2013

Fisetin is a naturally occurring flavonoid with some anti-cancer and anti-inflammation capabilities. In this study, we perform docking studies between human c-Jun N-terminal kinase 1 (JNK 1) and fisetin and proposed a binding model of fisetin and JNK 1, in which the hydroxyl groups of the B ring and oxygen at the 4-position of the C ring play key roles in binding interactions with JNK. Fluorescence quenching and saturation-transfer difference (STD) NMR experiments showed that fisetin exhibits good binding affinity to JNK, $1.32{\times}10^8M^{-1}$. The anti-inflammatory activity of fisetin was also investigated. Fisetin significantly suppressed tumor necrosis factor, the NO production, and macrophage inflammatory cytokine release in LPS-stimulated RAW264.7 mouse macrophages. We found that the anti-inflammatory cascade of fisetin was mediated through the JNK, and cyclooxygenase (COX)-2 pathways. Our findings suggest the potential of fisetin as an anti-inflammatory agent.

• Journal of Advanced Research in Ocean Engineering
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• 제2권2호
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• pp.48-60
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• 2016

Accurate simulation of free-surface wave flows around a ship is very important for better hull-form design. In this paper, a computational fluid dynamics (CFD) code which is based on the open source libraries, OpenFOAM, was developed to predict the wave patterns around a ship. Additional anti-diffusion source term for minimizing a numerical diffusion, which was caused by convection differencing scheme, was considered in the volume-fraction transport equation. The influence of the anti-diffusion source term was tested by applying it to free-surface wave flow around the Wigley and KCS model ships. In results, the wave patterns and hull wave profiles of the Wigley and KCS model ships for various anti-diffusion coefficients showed quite close patterns. While, the band width of the water volume-fraction values between 0.1 to 0.9 at the Wigley and KCS model hull surfaces was narrowed by considering the anti-diffusion term. From the results, anti-diffusion source term decreased free-surface smearing.

• 인터넷정보학회논문지
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• 제12권5호
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• pp.47-61
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• 2011

최근 사이버 범죄의 증가와 그 대상 시스템의 다양화로 인하여 디지털 포렌식의 중요성이 커지고 있다. 일부 시스템들은 전원이나 네트워크를 차단하지 않고 수사하는 live forensic의 방법을 채택하고 있는데, 인터넷 사용이 일반화됨에 따라 live forensic 방법이 채택되는 횟수가 증가하고 있다. 그러나 live forensic 기술이 상당한 발전을 거듭하였음에도 불구하고 원격으로 접근하여 행해지는 Anti-forensic 행위에는 여전히 취약한 실정이다. 이와 같은 문제를 해결하기 위하여 첫 번째로 우리는 Anti-forensic 행위를 5개의 계층으로 분류하고 각 계층별로 가능한 Anti-forensic 행위의 시나리오를 생성하는 방법을 제안하였다. 두 번째로 fuzzy 전문가 시스템을 제안하여 효과적으로 Anti-forensic 행위를 탐지할 수 있도록 하였다. 몇몇 Anti-forensic 행위에 사용되는 명령어들은 일반적인 시스템 관리를 위하여 사용되는 명령어와 매우 유사하다. 따라서 우리는 fuzzy logic을 사용하여 모호한 데이터를 다룰 수 있도록 하였다. 미리 정의된 시나리오에서 명령어와 옵션 및 인자 값을 이용하여 룰을 생성하고 fuzzy 전문가 시스템에 이 룰을 학습하도록 하여 유사한 행위가 탐지되었을 때 추론을 통하여 수사관에게 얼마나 위험한 행위인지 알려준다. 이 시스템은 live forensic 수사가 진행될 때 발생할 수 있는 Anti-forensic 행위를 실시간으로 탐지할 수 있도록 하여 증거 데이터의 무결성을 유지하도록 한다.

• 대한물리치료과학회지
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• 제9권2호
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• pp.111-122
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• 2002

In oriental medicine, manual-acupuncture and electroacupuncture (EA) have been widely utilized to cure several inflammatory diseases such as arthritis. We designed this experiment to find neurochemical mechanism related to electroacupuncture induced anti-inflammatory effect on mouse air pouch model. EA with both low frequency (1 Hz) and high frequency (120 Hz) was treated after induction of inflammation in air pouch using injection of zymosan. To verify the role of opioid system in electroacupuncture-induced anti-inflammatory effect, naloxone (10 mg/kg) was pretreated. In addition, idazoxan (5 mg/kg) was pre-treated to evaluate the possible effect of endogenous adrenergic system in autonomic system on EA induced anti-inflammatory effect. As results of this study, naloxone pretreatment did not change the anti-inflammatory effect evoked by high frequency EA, while low frequency EA(1 Hz) induced anti-inflammatory effect was dramatically suppressed by naloxone pretreatment. These data indicated that endogenous opioid system might be extensively involve in anti-inflammatory effect evoked by not high frequency, but low frequency EA. However, idazoxan pretreatment did not produce any modulatory effect on both low and high frequency EA induced anti-inflammatory effect, suggesting that EA induced anti-inflammatory effect was not mediated by endogenous adrenergic system. In conclusion, these data strongly suggested that EA induced anti-inflammatory effect is mediated by endogenous opioid system, not endogenous adrenergic system.

• 한국음향학회지
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• 제19권2호
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• pp.90-94
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• 2000

본 논문에서는 HMM 기반의 인식시스템에서 신뢰성이 낮은 입력패턴을 거절하기 위해 필요한 임계 문턱값을 효과적으로 추정하는 방법을 제안한다. Anti-model을 이용한 거절방식은 통계적 가설(statistical hypothesis)에 근거하여 주어진 입력에 대한 HMM과 anti-model 간의 유사도를 임계 문턱값과 비교하여 거절 여부를 결정한다. HMM은 각 클래스 별로 출력값의 편차가 심하게 나타나므로 일률적인 문턱값을 적용하는 것이 어렵다. 본 논문에서는 각 클래스 별로 HMM 생성을 위해 사용된 학습데이터를 이용하여 클래스에 종속적인 임계 문턱값을 추정하는 방법을 제안한다. 각 클래스에서의 임계 문턱값은 학습데이터에 대한 HMM과 anti-model 간의 유사도로부터 추정된다. 실험에서는 HMM 기반의 3차원 손 제스처 인식시스템에 대해 제안한 문턱값 추정방법을 적용하여 거절검사를 수행하였다. 실험 결과로부터 제안한 임계 문턱값 추정방법이 anti-model 구현 방식에 관계없이 적용이 가능하고 추정된 문턱값을 이용하여 부적절한 입력 패턴을 적절하게 거절할 수 있음을 확인할 수 있었다.

• 대한화장품학회지
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• 제29권2호
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• pp.37-65
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• 2003

새로운 주름개선 효능물질을 개발하기 위하여 여러 가지 세포배양 모델을 이용한 in vitro 효능물질의 검색방법을 이용하여 후보물질을 도출하고 있다. 이처럼 In vitro에서 선별된 주름개선 후보물질은 사람피부에 직접 도포 하였을 때 in vitro에서 와 는 달리 주름개선 효능이 없는 경우가 많이 있다. 즉 in vitro 검색결과와 사람피부에서의 효능은 현실적으로 큰 차이를 보인다는 것이다. 그러나 모든 효능 후보물질을 사람 피부에 직접 검사할 수는 없는 현실적 어려움이 있다. 이러한 애로점을 해결하기 위하여, 동물모델이나, 인공피부배양모델 등을 이용하는 추세이다. 이번 강의에서, 본인은 동물모델과 in vitro에서 효능 측정방법을 상세하게 리뷰 하고 각 방법에 대해 토의할 것이며, 임상 전 시험 결과를 보고 할 것이다. 그 중 특히 동물 모델은 주름개선 효능물질의 효능을 평가하는데 좋은 대상이 될 것이다.

• Journal of Pharmaceutical Investigation
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• 제36권5호
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• pp.309-314
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• 2006

The multicellular layers(MCL) of human cancer cells is a three dimensional(3D) in vitro model for human solid tumors which has been used primarily for the assessment of avascular penetration of anti-cancer drugs. For anti-cancer drugs with penetration problem, MCL represents a good experimental model that can provide clinically relevant data. Calcein-AM is a fluorescent dye that demonstrates the cellular vitality in a graded manner in cancer cell culture system. In the present study, we evaluated the use of calcein-AM for determination of anti-proliferative activity of anti-cancer agents in MCL model of DLD-1 human colorectal cancer cells. Optical sectioning of confocal imaging was compromised with photonic attenuation and penetration barrier in the deep layers of MCL. By contrast, fluorescent measurement on the cryo-sections provided a feasible alternative. Cold pre-incubation did not enhance the calcein-AM distribution to a significant degree in MCL of DLD-1 cells. However, the simultaneous determination of drug penetration and cellular vitality appeared to be possible in drug treated MCL. In conclusion, these data suggest that calcein-AM can be used for the simultaneous determination of drug-induced anti-proliferative effect and drug penetration in MCL model.

• 한국소음진동공학회논문집
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• 제16권11호
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• pp.1108-1114
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• 2006

Underwater acoustic transducers can be exposed to a underwater explosive shock caused by various types of underwater weapon. So, a robust anti-shock design is required for transducers to endure the underwater explosive shock. To check the anti-shock characteristics of a transducer, underwater explosive shock test is needed. The conditions of underwater explosive shock test are set up referring to various oversea explosive shock test specifications, and the explosive shock pressure values are calculated according to those conditions. Transient analyses art: carried out for two kinds of underwater acoustic transducer model to verify the anti-shock characteristics. The applied model has robust anti-shock characteristics enough to endure the explosive shock up to 2300 psi. In the future, the transducer design should be certified through the fields test, and modified if needed.

• 경영과정보연구
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• 제26권
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• pp.61-90
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• 2008

The purpose of this paper is to investigate the effects of anti-smoking advertising on attitude toward anti-smoking and behavioral intention to quit smoking in terms of audience's involvement with anti-smoking and knowledge on smoking. For this, a total of 10 hypothesis were established and statistically tested. According to the results, all but hypothesis 1-1(attitude toward anti-smoking is more favorable in the high involvement condition than in the low involvement condition) were unfortunately rejected. These results can be justified by theoretical explanations such as Hierarchy Effects Model or Elaboration Likelihood Model. In addition, some methodological reasons were provided as well.

• 한국군사과학기술학회지
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• 제21권6호
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• pp.877-885
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• 2018

The anti-submarine helicopter is the most effective weapon system in anti-submarine warfare. Recently changes in the introduction of the anti-submarine warfare sonar system are expected to operate multi-static sonar equipment of the anti-submarine helicopter. Therefore, it is required to study the operational concept of multi-static of anti-submarine helicopter. This paper studies on the optimal search of multi-static based on anti-submarine helicopter considering Furthest On Circles(FOC). First, the deployment of the sensors of the anti-submarine helicopter is optimized using genetic algorithms. Then, the optimized model is extended to consider FOC. Finally, the proposed model is verified by comparing pattern-deployment the search method in Korean Navy.