• 혜화의학회지
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• 제14권2호
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• pp.55-70
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• 2005

In oriental medicine, Gamibangpungtongsungsan (KBTS) has been used as a therapeutic agent for the treatments of acute stage of cerebrovascular diseases and hypertension. In the present study, underlying mechanism on KBTS effects was investigated using spontaneously hypertensive rats (SHR) by determining related parameters such as blood pressure, heart-beat rates, and hormones and plasma constituents. The major finding are summarized as follows. 1. KBTS treatment at concentrations lower than $125\;{\mu}g/m{\ell}$ did not show any cytotoxicity on cultured human fibroblast cells. 2. KBTS treatment in SHR significantly decreased blood pressure and heart-beat rate compared with untreated control. 3. KBTS treatment in SHR decreased aldosterone levels in the blood compared with untreated control, but the difference was not statistically significant. 4. KBTS treatment in SHR significantly decreased dopamine, norepinephrine and epinephrine levels in the blood compared with untreated control. 5. KBTS treatment in SHR decreased plasma ion concentrations such as Na+, K+, Ca2+, Cl- compared with untreated control; decreases in Na+ and Cl- were statistically significant. 6. KBTS treatment in SHR significantly decreased TNF-$\alpha$, IL-6, and IL-10 levels in the blood compared with untreated control. Thus, the present data show evidence on anti-hypertension activity of KBTS in an experimental animal system, which can provide further insights into the development of anti-hypertension therapeutic agents.

• 생약학회지
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• 제49권3호
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• pp.240-245
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• 2018

The fruits of Eleutherococcus sessiliflorus (Rupr. & Maxim.) S. Y. Hu (Araliaceae), as edible fruits, were traditionally used for ingredients of wine or tea in Eastern Asia. In addition to, the fruits of E. sessiliflorus were known for having antioxidant and anti-inflammatory effects. Recently, we investigated that the ethanolic extracts of E. sessiliflorus fruits (DHP1501) have effects on hypertension via vasorelaxation and decrease of blood pressure. In the present study, we investigated that the gene and protein expression levels of endothelial nitric oxide synthase (eNOS) was increased by treatment of DHP1501 in HUVECs. Moreover, we confirmed the angiotensin converting enzyme inhibitory activity of DHP1501 through in vitro tasks. Therefore, DHP1501 could be a candidate of functional food for alleviating hypertension.

• 생약학회지
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• 제13권1호
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• pp.26-32
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• 1982

In order to investigate the effect of 'Whangryonhaedok-Tang' on the anti-inflammatory, ileum of mice, blood pressure, blood glucose, total cholesterol and antibacterial activity, those pharmacological actions were conducted. The results of this studies were summarized as follows; Rat hind paw edema induced by acetic acid was inhibited. In mice, tone of intestinal smooth muscle was suppressed with the treatment of sample and intestinal contractions induced by $BaCl_2$ was inhibited. Hypertensive effect was not observed in rabbits but was observed by the water extract of 'Whangryonhaedok-Tang' excluded Phellodendri Cortex in rabbits. The increased blood glucose levels in alloxandiabetic rabbits were significantly decreased by the oral administration of the sample I after 6 days. The total cholesterol levels in alloxan-induced rabbits were significantly decreased by the oral administration of the sample I after 9 days. Antibacterial activity was observed. In addition, we thought the pharmacological actions were significantly noted by the sample III (Methanol soluble fraction) because of the purification of effective components.

• 한국수산과학회지
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• 제57권2호
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• pp.137-144
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• 2024

In this study, we investigated the antioxidant and physiological activities of the Korean marine algae, Enteromorpha compressa. Solvent extracts of E. compressa were prepared using 70% ethanol, 80% methanol, and water, with extraction yields ranging from 9.55% to 25.67%. The total polyphenol and flavonoid contents ranged from 20.76-28.41 mg/g and 2.56-18.59 mg/g, respectively. Compared with the water extract, the ethanol and methanol extracts were found to have higher antioxidant activities. All three extracts were found to promote alcohol dehydrogenase and aldehyde dehydrogenase activities in a concentration-dependent manner, whereas the methanol and ethanol extracts were established to have the highest angiotensin-converting enzyme (ACE) inhibitory activity (IC₅₀=1.40 ㎍/mL) and β-secretase inhibitory activity (IC₅₀=0.17 ㎍/mL), respectively. These findings thus indicate that E. compressa could have beneficial application as a supplementary antioxidant and functional constituent in food and pharmaceutical materials.

• 생약학회지
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• 제25권1호
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• pp.51-58
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• 1994

The studies were conducted to investigate the anti-diabetic activities on the hyperglycemia induced by streptozotocin in rats, anti-hypertensive activities in SHR, anti-fatigue and decrease of body weight activities in mice by Kyung Ok-Ko water extract and drink, which is a traditional preparation in Korea. 1. The blood glucose levels of streptozotocin-induced hyperglycemic rats were dose-dependently decreased by administrations of various doses(300, 600, 1200 mg/kg) of Kyung Ok-Ko water extract. Particularly, administration of 600, 1200 mg/kg were significantly shown to decrease glucose levels comparing with control group. 2. The serum total cholesterol levels of streptozotocin-induced hyperglycemic rats were dose-dependently decreased by administrations of various doses(300, 600, 1200 mg/kg) of Kyung Ok-Ko water extract. Particularly, administration of 600, 1200 mg/kg were significantly shown to decrease total cholesterol levels comparing with control group. 3. The serum triglyceride levels of streptozotocin-induced hyperglycemic rats were dose-dependently decreased by administrations of various doses(300, 600, 1200 mg/kg) of Kyung Ok-Ko water extract. Particularly, administration of 600, 1200 mg/kg were significantly shown to decrease triglyceride levels comparing with control group. 4. The blood pressure in SHRs were dose-dependently lower descended by administration of Kyung Ok-Ko water extract 300, 600, and 1200 mg/kg, respectively. 5. The swimming time of Kyung Ok-Ko water extract(100, 200, 400 mg/kg) and drink(0.7, 1.4, 2.1 ml/kg) were significantly extended to all experimental group dose-dependantly. 6. The decrease of body weight of Kyung Ok-Ko water extract(100, 200, 400 mg/kg) and drink(0.7, 1.4, 2.1 ml/kg) were significantly evaluated dose-dependently in all experimental group.

• 식품과학과 산업
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• 제51권4호
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• pp.278-301
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• 2018

최근 들어 먹거리와 건강에 대한 사람들의 관심이 점점 높아지면서 이에 관한 정보 또한 중요시 되고 있다. 우리나라 전체 사회가 고령화 사회로 접어들면서 환경의 악화로부터 오는 질병과 생활 습관병의 증가를 자기 자신의 문제로 받아들이는 사람들이 늘고 있기 때문이다. 의료 분야는 치료 중심에서 예방 의학으로 관심이 높아지고 있지만 예방약이라고 할 수 있는 약품은 매우 적다. 암, 동맥경화, 당뇨병 같은 성인병을 예방하려면 약에 의한 예방이 아닌 먹거리에 의한 예방을 적극적으로 도입시킬 필요가 있다. 해양은 육상과는 다른 특이한 생태계를 이루는 환경 때문에 적자생존의 경쟁 속에서 살아남기 위하여 특히 물리적 방어 능력이 부족한 해양생물의 2차 대사산물은 육상생물의 그것과는 상이한 화학적 특성을 가진다. 이러한 다양한 2차 대사산물은 화학적 방어 수단의 일환으로 생성되는 것으로 알려져 있는데 이들 물질이 인체나 다른 포유동물에 투여되면 강력한 생리활성을 발현하는 경우가 많다. 이러한 이유들 때문에 해양생물로부터 새로운 생리활성 선도물질을 개발하여 인류의 건강 보전에 이용하고자 하는 연구가 최근 각광받고 있다. 해조류, 어패류 및 수산가공부산물(폐기물)에서 기능성을 나타내는 단백질 가수분해물 및 펩티드의 제조 방법 및 항균, 항산화, 심장보호(항고혈압, 항동맥경화 및 항응고), 면역조절, 항당뇨, 식욕억제 및 신경보호 활성과 같은 여러가지 생리 기능성에 대하여 살펴보았다. 무엇보다도 해양단백질 가수분해물 및 펩티드가 다양한 생리활성을 갖고 있어 건강식품 및 식의약 산업에서 응용이 가능하고 원료인 해조류 및 수산가공부산물이 대부분 미이용자원이므로 관련기업에서의 상품화 개발이 이루어질 것으로 기대된다. 최근에 소비자나 환자들도 의약품에 대한 바람직하지 않은 부작용을 의식하고 있고 화학적으로 합성된 의약품을 기피하는 경향이 있고 자연식품이나 천연 생리기능성 물질에 대한 욕구로 자가치료에 대한 사고방식이 높아지고 있어 의약품 보다는 효능이 다소 낮을지라도 어떤 질병의 예방이나 치료를 위해 특수한 생리기능성 물질의 섭취는 더욱 더 인기를 끌게 될 것으로 기대된다. 그러나 단백질 가수분해물이나 펩티드가 신체에서 나타내는 상태와 생리기능성 효과 사이에 상호관계를 충분히 밝혀져야 하며 이들을 이용한 제품의 보다 확실한 생리기능성의 작용 메커니즘과 임상에서의 효능의 확인이 이루어져야 하며 마켓팅을 위해서는 무엇보다도 공인기관인 식약처 또는 FDA에 인증을 받아야 할 것이다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 춘계학술발표회
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• pp.338-339
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• 2011

• The Korean Journal of Physiology and Pharmacology
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• 제17권1호
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• pp.31-36
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• 2013

The present study was undertaken to investigate the influence of eupatilin on vascular smooth muscle contractility and to determine the mechanism involved. Denuded aortic rings from male rats were used and isometric contractions were recorded and combined with molecular experiments. Eupatilin more significantly relaxed fluoride-induced vascular contraction than thromboxane $A_2$ or phorbol ester-induced contraction suggesting as a possible anti-hypertensive on the agonist-induced vascular contraction regardless of endothelial nitric oxide synthesis. Furthermore, eupatilin significantly inhibited fluoride-induced increases in pMYPT1 levels. On the other hand, it didn't significantly inhibit phorbol ester-induced increases in pERK1/2 levels suggesting the mechanism involving the primarily inhibition of Rho-kinase activity and the subsequent phosphorylation of MYPT1. This study provides evidence regarding the mechanism underlying the relaxation effect of eupatilin on agonist-induced vascular contraction regardless of endothelial function.

• The Korean Journal of Physiology and Pharmacology
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• 제20권1호
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• pp.69-74
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• 2016

The present study was undertaken to investigate the influence of cardamonin on vascular smooth muscle contractility and to determine the mechanism(s) involved. Denuded aortic rings from male rats were used and isometric contractions were recorded and combined with molecular experiments. Cardamonin significantly relaxed fluoride-, phenylephrine-, and phorbol ester-induced vascular contractions, suggesting that it has an anti-hypertensive effect on agonist-induced vascular contraction regardless of endothelial nitric oxide synthesis. Furthermore, cardamonin significantly inhibited the fluoride-induced increase in pMYPT1 level and phenylephrine-induced increase in pERK1/2 level, suggesting inhibition of Rho-kinase and MEK activity and subsequent phosphorylation of MYPT1 and ERK1/2. This study provides evidence that the relaxing effect of cardamonin on agonist-induced vascular contraction regardless of endothelial function involves inhibition of Rho-kinase and MEK activity.

• BMB Reports
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• 제51권12호
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• pp.611-612
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• 2018

$C1q/TNF-{\alpha}-Related$ Protein 1 (CTRP1) has recently been shown to act as a blood pressure regulator, as it induces vasoconstriction. In the aorta, CTRP1 facilitates recruitment of angiotensin II receptor 1 (AT1R) to plasma membrane, through activation of the AKT/AS160 signaling pathway. This leads to activation of the Ras homolog gene family (Rho)/Rho kinase (ROCK) signaling pathway, resulting in vasoconstriction. Accordingly, mice overexpressing Ctrp1 have hypertensive phenotype. Patients with hypertension also display higher circulating CTRP1 levels, compared to healthy individuals, indicating that excessive CTRP1 may affect development of hypertension. Conversely, CTRP1 is regarded as an 'innate blood pressure modulator' because CTRP1 increases blood pressure under dehydration to prevent hypotension. Mice lacking Ctrp1 fail to maintain normotension under dehydration conditions, resulting in hypotension, suggesting that CTRP1 is an essential protein for maintaining blood pressure homeostasis. In conclusion, CTRP1 is a novel, anti-hypotensive vasoconstrictor that increases blood pressure during dehydration-induced hypotension.