• KSBB Journal
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• v.28 no.5
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• pp.310-314
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• 2013

Antifreeze peptide from Myoxocephalus octodecemspinosus was overexpressed and purified in Escherichia coli. Green fluorescence protein-AFP chimera was constructed by integrating gfp and afp genes. Produced GFP-AFP chimera protein was purified using polyhistidine tag which was inserted at C-terminus. By addition of GFP-AFP chimera protein, freezing point of elution buffer was decreased from $-13^{\circ}C$ to $-20^{\circ}C$. This result suggested that GFP-AFP chimera can be considered as a potential candidate of novel inhibitor for gas hydrates.

• International Journal of Highway Engineering
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• v.3 no.4 s.10
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• pp.105-116
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• 2001

This study was performed to evaluate the characteristics of waste foundry sand (WFS) and the asphalt mixture made of a foundry waste sand. To estimate the applicability of WFS, chemical and physical properties were measured by XRF(X-ray fluorescent), and SEM(Scanning electronic microfilm). To improve the stripping resistance of WFS asphalt mixture, anti-stripping agents (a hydrated lime and a liquid anti-stripping agent) were used. To improve tensile properties and durability of WFS asphalt concrete mixture, LDPE(low-density polyethylene) was used as an asphalt modifier Marshall mix design, indirect tensile strength, tensile strength ratio(TSR) after freezing and thawing, moisture susceptibility and wheel tracking tests were carried out to evaluate performance of WFS asphalt concrete. Comparing with conventional asphalt concrete, WFS asphalt concretes showed similar or the better qualify in mechanical properties, and satisfied all specification limits. Therefore, it Is concluded that waste foundry sand can be recycled as an asphalt pavement material.

• Fire Science and Engineering
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• v.30 no.1
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• pp.49-56
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• 2016

This paper numerically examines, straight and twisted electrical heat trace installations for their anti-freezing effects on water inside a pipe. The unsteady incompressible Navier-Stokes equations coupled with an energy equation were solved to compare the two installation methods. The heat conduction of the pipe with a heat source interacts with the natural convection of the water, and the conjugate heat transfer was considered using a commercial code (ANSYS-FLUENT) based on a SIMPLE-type algorithm. Numerical experiments, were done to investigate the isotherms and the vector fields in the water region to extract the evolutions of the minimum and maximum temperatures of the water inside the pipe. There was no substantial difference in the anti-freezing effects between the straight and twisted. Therefore, the straight installation is recommended after considering the damage and short circuit behavior of the electrical heat trace.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2019.05a
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• pp.283-284
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• 2019

In the last decades patient's suffering with Parkinson's disease is increasing at a rapid rate and as per prediction it will grow more rapidly as old age population is increasing at a rapid rate through out the world. As the performance of wearable sensor based approach reached to a new height as well as powerful machine learning technique provides more accurate result these combination has been widely used for assessment of various neurological diseases. ON state is the state where the effect of medicine is present and OFF state the effect of medicine is reduced or not present at all. Classification of ON/OFF state for the Parkinson's disease is important because the patients could injure them self due to freezing of gait and gait related problems in the OFF state. in this paper wearable sensor based approach has been used to collect the data in ON and OFF state and machine learning techniques are used to automate the classification based on the gait pattern. Supervised machine learning techniques able to provide 97.6% accuracy while classifying the ON/OFF state.

• International Journal of Highway Engineering
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• v.11 no.4
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• pp.143-152
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• 2009

The purpose of this study is to evaluate the freezing index and frost penetration depth. The freezing index and frost penetration depth were analyzed using air temperature and temperature profile of pavement system in Korea LTPP-SPS(Long Term Pavement Performance-Specific Pavement Study) site. The predicted frost penetration depth were then compared with the measured one from the LTPP sites. And the trend of annual freezing index was analyzed using the temperature data of meteorological stations located in the vicinity of Korea LTPP-SPS site. The result showed that the freezing index was rapidly decreased since 1987, and it was known that the use of freezing index determined from the past 30 years temperature data could cause the over estimates in the pavement thickness design. The temperature profile measured at 3 sections of LTPP-SPS sites showed that the temperature of subbase layer was above $0^{\circ}C$, even though anti-frost layers were found in these sections. Comparing the measured and calculated frost depth, the frost depth calculated from the subgrade frost penetration permissible method showed a similar trend with the measured frost depth.

• Clinical and Experimental Reproductive Medicine
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• v.31 no.1
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• pp.75-81
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• 2004

Objective: The purpose of this study was to evaluate the efficacy and effect of various cryopreservation method on the survival and the cytoskeletal stability of metaphase II mouse oocyte. Methods: Mouse ovulated oocytes were collected and cryopreserved by a modified slow-freezing method with 1.5 M 1, 2-propanediol (PrOH)+0.1 M sucrose or by vitrification using cryo loop and EM grid with 40% ethylene glycol+0.6 M sucrose. Four hours after thawing, intact oocytes were fixed and stained with fluorescein isothiocyanate (FITC)-conjugated monoclonal anti-$\beta$-tubulin antibody to visualize spindle and propidium iodide (PI) to visualize chromosome. Spindle morphology was classified as follows: normal (barrel-shaped), slightly and absolute abnormal (multipolar or absent). Results: Survival rate of the frozen-thawed oocytes in vitrification group was significantly higher than that of slow-freezing group (62.7% vs. 24.4%, p<0.01). Vitrification with cryo loop showed significantly higher survival rate than that with EM grid (67.7% vs. 53.5%, p<0.05). On the other hand, proportion of normal spindle and chromosome configurations of the frozen-thawed oocytes between two vitrification group was not significantly different. Conclusion: For mouse ovulated oocytes, vitrification with cryo loop may be a preferable procedure compared to slow-freezing method. Further study should be needed to investigate developmental competency of frozen-thawed mouse oocytes.

• Journal of the Korean Solar Energy Society
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• v.25 no.4
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• pp.37-44
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• 2005

An experimental study was conducted to analyze performance of a heating system with variation of control logic of the system. The system uses a solar as heat source and composed with heat pump that uses R-22 as working fluid. The difference between the developed system and the commercially available heating system is working fluid. The solar assisted heating system which was widely distributed in the market uses water as a working fluid. It could be freezing in case of the temperature drops down under freezing point. The anti-freezing fluids such as methyl-alcohol or ethylene-glycol are mixed with the water to protect the freezing phenomena. However, the system developed in this study uses a refrigerant as a working fluid. It makes the system to run under zero degree temperature conditions. Another difference of the developed system compare with commercial available one is auxiliary heating method. The developed system has removed an auxiliary electric heater that has been used in conventional solar assisted heating system. Instead of the auxiliary electric heater, an air source heat exchanger which generally used as an evaporator of a heat pump was adapted as a backup heating device of the developed system. As results, an efficiency of the developed system is higher than a solar assisted heat pump with auxiliary electric heater. The merit of the developed system is on the performance increment when the system operates at a lower solar energy climate conditions. In case of the developed system operates at a normal condition, COP of the solar collector driven heat pump is higher than the air source heat exchanger driven heat pump's.

• Magazine of the Korea Concrete Institute
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• v.10 no.4
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• pp.205-212
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• 1998

Frost damage of a storage tank for chemical admixtures caused by a low temperature in winter and quality deterioration of chemical admixtures have been often reported. However, there have been few regulations and researches related to the frost damage of chemical admixtures and facilities. Therefore, this paper is intend to investigate not only, the freezing points of chemical admixtures such as AE admixtures and water-reducing AE admixtures, etc., but also the quality variation of concrete used with chemical admixtures before freezing and after freeze-thaw cycles. According to the results of experiments, most chemical admixtures except anti-freezing agent and accelerating water-reducing AE admixtures should be kept from being frozen in low winter temperatures. However, full agitation of frozen chemical admixtures dose not cause the problems of concrete related to the quality of chemical admixtures.

• Journal of the Korean Data and Information Science Society
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• v.27 no.1
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• pp.67-74
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• 2016

Due to the character of the climate of Korea, the pavement of a road is Influenced by freezing in winter season and thawing in thawing season. In the last few years, several articles have been devoted to the study to minimize the damage of freezing and thawing action. The purpose of this paper is to identify appropriacy of factors that influence road pavement thickness. We conduct the decision tree analysis on the field data of road pavement. The target variable is 'Frost penetration'. This value was calculated from the temperature data. The input variables are 'Region', 'Type of road pavement', 'Anti-frost layer', 'Month' and 'Air temperature'. The region was divided into 9 regions by freezing index $350{\sim}450^{\circ}C{\cdot}day$, $450{\sim}550^{\circ}C{\cdot}day$, $550{\sim}650^{\circ}C{\cdot}day$. The type of road pavement has three-section such as area of cutting, boundary area of cutting and bankin, lower area of banking. As the result, the variables that influence 'Frost penetration' are Month, followed by anti-frost layer, air temperature and region.

• Proceedings of the SAREK Conference
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• 2007.06a
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• pp.117-117
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• 2007