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http://dx.doi.org/10.7841/ksbbj.2013.28.5.310

Overexpression of GFP-AFP Chimera Protein using Recombinant Escherichia coli and Analysis of Anti-freezing Characteristics  

Ko, Ji-Seun (Department of Chemical Engineering, University of Ulsan)
Hong, Soon Ho (Department of Chemical Engineering, University of Ulsan)
Publication Information
KSBB Journal / v.28, no.5, 2013 , pp. 310-314 More about this Journal
Abstract
Antifreeze peptide from Myoxocephalus octodecemspinosus was overexpressed and purified in Escherichia coli. Green fluorescence protein-AFP chimera was constructed by integrating gfp and afp genes. Produced GFP-AFP chimera protein was purified using polyhistidine tag which was inserted at C-terminus. By addition of GFP-AFP chimera protein, freezing point of elution buffer was decreased from $-13^{\circ}C$ to $-20^{\circ}C$. This result suggested that GFP-AFP chimera can be considered as a potential candidate of novel inhibitor for gas hydrates.
Keywords
Antifreeze peptide; Hydrate; Green fluorescence protein; Escherichia coli;
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