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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Overexpression of GFP-AFP Chimera Protein using Recombinant Escherichia coli and Analysis of Anti-freezing Characteristics

재조합 대장균을 이용한 GFP-AFP Chimera 단백질 과량발현 및 특성 파악연구

  • Ko, Ji-Seun (Department of Chemical Engineering, University of Ulsan) ;
  • Hong, Soon Ho (Department of Chemical Engineering, University of Ulsan)
  • 고지선 (울산대학교 화학공학부) ;
  • 홍순호 (울산대학교 화학공학부)
  • Received : 2013.09.12
  • Accepted : 2013.10.17
  • Published : 2013.10.30
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Abstract

Antifreeze peptide from Myoxocephalus octodecemspinosus was overexpressed and purified in Escherichia coli. Green fluorescence protein-AFP chimera was constructed by integrating gfp and afp genes. Produced GFP-AFP chimera protein was purified using polyhistidine tag which was inserted at C-terminus. By addition of GFP-AFP chimera protein, freezing point of elution buffer was decreased from $-13^{\circ}C$ to $-20^{\circ}C$. This result suggested that GFP-AFP chimera can be considered as a potential candidate of novel inhibitor for gas hydrates.

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References

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