• KSBB Journal
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• 제17권2호
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• pp.203-206
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• 2002

신체성장은 세포수가 증가하고 세포의 크기가 커지면서 신체의 크기가 증가하는 것으로서 몸을 구성하는 성분인 단백질이 증가를 수반하고 골격과 근육이 커지며 이득의 기능도 강화된다. 사람의 키가 커진다는 것은 팔과 다리의 긴 뼈가 늘어나야 하는 것인데 이와같은 성장은 호르몬의 작용에 의해서 이루어진다. 본 연구에서는 이와같은 성장에 미치는 영향을 검토하기 위해 성장호르몬 및 IGF-1의 분비촉진 여부를 확인하였다. 체외 래트 성장호르몬의 분비량은 88 - 99 ng/mL으로 대조군에 비해 약 6배 이상의 성장호르몬이 분비되었다. 또한 생체내 IGF-1 분비촉진 효과실헐에서는 YGF 투여 후 8시간에서 1140 ng/mL 정도로 대조군에 비해 현저하게 높은 혈중 IGF-1의 농도가 유지되었다. YGF를 장기 복용시킨 경우 실제로 대퇴부 배의 길이성장이 약 6% 정도 이루어졌음을 확인할 수 있었다. 따라서 본 실험을 통해 확인된 성장호르몬 및 IGF-1 분비촉진을 유발하는 식물 추출물 YGF에 대해 좀더 연구개발하여 키 성장이 저조한 어린이 및 청소년에게 신체성장에 도움이 될 수 있도록 되었으면 한다.

• 생명과학회지
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• 제10권6호
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• pp.570-576
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• 2000

This study was designed to investigate the effects of mulberry (Morus alba L.) leaf extract (MLE) on oxygen radicals and their scavenger enzymes in brain membranes of rats. Sprague-Dawley (SD) male rats (160$\pm$10 g) were fed basic diet (control group), and experimental diets (MLE-100 and MLE-300 groups) added 100 and 300 mg/kg BW/day for 6 weeks. Hydroxyl radical (.OH) lecels resulted in significant decreases (13.4% and 21.1%, 12.0% and 13.4%, respectively) in brain mitochondria and microsome of MLE-100 and MLE-300 groups compared with control group. Superoxide radical ($O_2$) levels were significantly decreased about 12% in brain cytosol of MLE-300 group compared with control group. Lipid peroxide (LPO) levels were effectively inhibited (18.1% and 12.3%, respectively) in brain mitochondria and microsomes of MLE-300 groups compared with control group. Oxidized protein (OP) levels were significantly decreased (14.2%, and 10.9%, respectively) in brain mitochondria and microsomes of MLE-300 groups compared with control group. Mn-SOD activities in brain mitochondria were significantly increased (13.5% and 18.6%, respectively) in MLE-100 and MLE-300 groups, and Cu/Zn-SOD activities in brain cytosol were also effectively increased (about 17.7%) in MLE-300 groups compared with control group. GSHPx activities in brain cytosol were remarkably increased (17.2% and 23.9%, respectively) in MLE-100 and MLE-300 groups compared with control group. These results suggest that anti-aging effect of mulberry leaf extract (MLE) may play a pivotal role in attenuating a various age-related changes in brain.

• 생명과학회지
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• 제10권4호
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• pp.340-346
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• 2000

This study was designed to investigate the effects of silk fibroin(Mw 500) powder (SFP) on oxygen radicals and the scavenger enzymes in brain membranes of rats. Spragu-Dawley(SD) male rats(160${\pm}$10g) were fed basic diet(control group), and experimental diets(SFP-2.5 and SFP-5.0 groups) added 2.5 and 5.0g/kg BW/day for 6 weeks. Hydroxyl radical($.$OH) levels resulted in a decreases(6.6% and 9.7%, 2.8% and 11.9%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group, but were significantly decreased in these membrances of SFP-5.0 group only. Superoxide radical (O2) levels were a slightly decreased (2.0% and 9.1%, respectively) in brain cytosol of SFP-2.5 and SFP-5.0 groups compared with control group. Lipid peroxide(LPO) levels were significantly decreased (12.9% and 21.9%, 13.2% and 22.5%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. Oxidized protein (OP) levels were significantly decreased (16.7% and 15.7%, respectively) in brain microsomes of SFP-2.5 and SFP-5.0 group compared with control group, but significantly difference between in brain mitochondria of these two groups could not be obtained. Mn-SOD activities were remarkably increased (11.2% and 24.2%, respectively) in mitochodria of SFP-2.5 and SFP-5.0 groups. CuZn-SOD activities were effectively increased (7.7% and 19.6%, respectively) in brain cytosol of SFP-2.5 and SFP-5.0 groups, but significant difference between control and SFP-2.5 groups could be not obtained. GSHPx activities were considerably increased (5.3% and 11.7%, respectively) in brain cytosol of SFP-2.0 and SFP-5.0 groups compared with control group. There results suggest that anti-aging effect of silk fibroin may play an effective learning and memory role in a attenuating a oxidative stress and increasing a scavenger enzyme activity in brain membranes.

• 생명과학회지
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• 제14권2호
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• pp.205-208
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• 2004

아위버섯을 약용과 식용으로 이용하고자 일반성분, 무기질, 구성아미노산, 유리 아미노산, 비타민 등을 분석검토한 결과 조지방이 8.0 g%, 탄수화물이 54.3 g%, 조단백질이 20.2 g%이었다. 무기물은 K가 2,337.0 mg%으로 가장 많이 함유 되어있고, Mg, Na, Zn, Ca, Fe의 순으로 함유되었다. 구성 아미노산은 glutamic acid가 13.65 %mol로 가장 많이 함유되어있고, aspartic acid, glysine, alanine, leucine, arginine, valine, lysine등의 순으로 17종의 아미노산을 함유하고 있으며 필수아미노산은 37,78 %mol이었다. 유리아미노산은 alanine이 547.97 $\mu\textrm{g}$%으로 가장 많이 함유되어 있고, phenylalanine, tyrosine, leucine 등의 순이며, 총 함유량은 2,737.38 $\mu\textrm{g}$%이고, 21종을 함유하고 있기 때문에 아위버섯의 맛이 좋을 것이라 생각되어 진다. 비타민의 경우 vitamin A가 0.12 mg%, vitamin B$_1$은 0.31 mg%, vitamin B$_2$는 0.68 mg%, vitamin C는 7.99 mg%, vitamin E는 316.88 mg%, vitamin D$_3$는 0.29 mg%을 함유하였고, 비타민의 총 함유량은 326.27 mg%이었다. 특히 vitamin C와 vitamin E가 많이 함유되어 노화방지에 좋으리라 생각된다.

• 한방비만학회지
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• 제15권2호
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• pp.93-103
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• 2015

Objectives: It was investigated the cytoprotective efficacies of isorhamnetin, a flavonoid originally derived from Hippophae rhamnoides L., against oxidative stress-induced apoptosis in C2C12 myoblasts. Methods: The effects of isorhamnetin on cell growth, apoptosis and reactive oxygen species (ROS) generation were evaluated by trypan blue dye exclusion assay, 4',6-diamidino-2-phenylindole staining and flow cytometry. The levels of apoptosis-regulatory and nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway-related proteins, and caspase activities (caspase-3 and -9) were determined by Western blot analysis and colorimetric assay, respectively. Results: Our results revealed that treatment with isorhamnetin prior to hydrogen peroxide ($H_2O_2$) exposure significantly increased the C2C12 cell viability and, indicating that the exposure of C2C12 cells to isorhamnetin conferred a protective effect against oxidative stress. Isorhamnetin also effectively attenuated $H_2O_2$-induced apoptosis and ROS generation, which was associated with the restoration of the upregulation of Bax and downregulation of Bcl-2 induced by $H_2O_2$. In addition, $H_2O_2$ enhanced the activation of caspase-9 and -3, and degradation of poly (ADP-ribose)-polymerase, a typical substrate protein of activated caspase-3; however, these events were almost totally reversed by pretreatment with isorhamnetin. Moreover, isorhamnetin increased the levels of heme oxygenase-1, a potent antioxidant enzyme, associated with the induction of Nrf2. Conclusions: Our data indicated that isorhamnetin may potentially serve as an agent for the treatment and prevention of muscle disorders caused by oxidative stress.

• 대한예방한의학회지
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• 제14권3호
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• pp.93-105
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• 2010

Objective : Recently the number of health functional food (HFF) made with Traditional Korean Medicine (TKM) herbs have been increasing. However, there was a lack of the functional and safety information on HFF. Thus, this study was performed to investigate perceptions and consumptions of the HFF in Daegu area. Method : We used the data of 'Survey in Daegu' in 2010 and analyzed characteristics of perceptions and intake conditions of HFF of 1,208 participated inhabitants. Result : Among the 1,208 respondents, 30.4% were male and 63.5% were female. Most respondents (69.3%) were between 30's and 50's decade. The recognition rate of HFF made with TKM herb appeared to 86.2%. The major route of acquiring HFF information was 'mass-media' (49.8%) ; the primary reason of using HFF was 'For health promotion and anti-aging' (69.2%). On the other hand, the recognition rate of adverse effects was 53.3%. As for intake effect, 41.5% were satisfied at HFF consumption, while 56.0% did not feel special effects through the HFF consumption. In regards to purchase place, 'HFF store' was most selected by 38.0%. 69.1% of respondents selected to TKM decoction, the reason is that it made according to TKM physician's diagnosis considered their health condition. Conclusion : HFF intake was purpose to health promotion, but HFF made with TKM was consumed without any information about that. In order that HFF be used properly to promote health, the scientific and reasonable information of HFF made with TKM herb is need for consumers.

• 한국잠사곤충학회지
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• 제41권3호
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• pp.135-140
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• 1999

This study was designed to investigate the effects of mulberry leaf extract (MLE) on oxygen radicals and their scavenger enzymes in serum of rats. Sprague-Dawley (SD) male rats (160${\pm}$10g) were fed experimental diets (MLE-100 and MLE-300 groups) added 100 and 300mg/kg BW/day for 6weeks. Triglyceride (TG) levels were significantly inhibited (10% and 20%) in MLE-100and MLE-300 groups, but there were no significant differences in total, LDL-and HDL- cholesterol levels in both MLE-100 and MLE-300 groups. Hydroxyl radical ($.$OH) formations resulted in a marked decreases(20∼25%) in MLE-100 and MLE-300 groups compared with control group, while superoxide radical (O2.-)and hydrogen peroxide formations resulted in a considerable decreases(7∼10% and 5∼10%) in MLE-100 and MLE-300 groups compared with control group. Lipid peroxide (LPO)and oxidized protein(>C=O group) productions resulted in a significant decreases (10% and 6∼10%) in MLE-100 and MLE-300 groups compared with control group. Superoxide dismutase (SOD)and catalase (CAT) activities were remarkably increased (30% and 40∼55%) in MLE-100 and MLE-300 groups, but glutathione peroxidase (GSHPX) activities were significantly increased (10∼15%) in MLE-100 and MLE-300 groups compared with control group. These results suggest that anti-aging effect of mulberry leaf extract (MLE) may play a pivotal role in attenuating a various agerelated changes.

• 한국균학회지
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• 제47권2호
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• pp.153-163
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• 2019

본 연구는 갯버들(Salix gracilistyla)의 화장품 소재로의 활용가능성을 판단하기 위해, 기존 일반적인 방법이 아닌 균사배양 방법을 통해 가능성을 확인하였고, 구체적으로는 대표적인 약용버섯인 불로초(Ganoderma lucidum) 균사의 배양을 통하여 그 효능을 확인하였다. 총 폴리페놀 및 플라보노이드 함량과 DPPH 라디칼소거능 분석결과에서는, 갯버들 추출물(SGE)이 불로초 균사배양 갯버들 추출물(GLSGE) 보다 높게 평가되었으나, 콜라게나제 활성 억제 평가에서는 불로초 균사배양 갯버들 추출물(GLSGE)이 갯버들 추출물(SGE)과 비교하여 상대적으로 낮게 평가되었다. 그럼에도 불구하고, 불로초 균사배양 갯버들 추출물(GLSGE)은 갯버들 추출물(SGE)을 처리한 경우보다, HDFn 세포의 생존율을 높은 수준으로 증가시켰으며, HDFn 세포의 제1형 프로콜라겐의 생합성 또한 매우 높은 수준으로 증가시키는 것이 확인되었다. 결과적으로 앞선 실험을 통해, 불로초 균사배양 갯버들 추출물이 항노화 활성을 가지는 기능성 화장품 소재로 활용 가능성이 있다고 사료되며, 기능성 소재의 효과를 더욱 증진시키기 위한 방안으로 불로초 균사의 배양이 효과적이라는 것을 의미한다.

• 한국응용과학기술학회지
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• 제37권6호
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• pp.1545-1555
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• 2020

In the present study, we investigated the antioxidant activity of Aucklandia lappa Decne (AL). Cell viability was measured in an MTT assay. Antioxidant effects were evaluated based on total polyphenol/flavonoid contents, ABTS radical scavenging activity, DPPH radical scavenging activity, SOD activity, and ROS content. AL was found not to be toxic at concentrations of 1 ㎍/mL, 10 ㎍/mL, and 100 ㎍/mL, respectively. The phenolic content was higher in AL-D than in AL-E, while the flavonoid content was higher in AL-E than in AL-D. AL-E exhibited higher ABTS radical scavenging activity than AL-D, and the EC50 values for BHA were 217.1 ㎍/mL in AL-D and 180.5 ㎍/mL in AL-E. AL-E also showed the highest DPPH radical scavenging activity. EC50 values for BHA were 114.2 ㎍/mL in AL-D and 95.8 ㎍/mL in AL-E. The SOD-like activity of AL-E was higher than that of AL-D. The EC50 values for ascorbic acid were 48.5 ㎍/mL in AL-D and 72.9 ㎍/mL in AL-E, indicating that both AL extracts have a SOD activity higher than that of ascorbic acid. AL-E reduced relatively more ROS than AL-D. With 100 ㎍/mL AL-E, the reduction level was almost similar to that of dexamethasone. Our results demonstrate that AL have antioxidant effects, and we believe that they could be very valuable as raw materials for anti-aging products, based on their antioxidant activity.