• Journal of Applied Biological Chemistry
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• 제51권1호
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• pp.7-12
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• 2008

The suitability of CPWs, by-products of the juice industry, was investigated as a source for the production of cosmeceuticals. Four kinds of CPWs, CW, CWE, CWER, and CWEA, were examined for their antioxidant potentials in terms of DPPH radical-scavenging ability for anti-wrinkle applications, inhibition of tyrosinase or melanin production for whitening products, and anti-inflammatory effects to treat various skin diseases such as atopic dermatitis and acne as well as for anti-bacterial activity against acne-inducing pathogens. Of the four extracts, CWER was the most potent tyrosinase inhibitor ($IC_{50}$ value: $109\;{\mu}g/mL$), and CWEA ($IC_{50}:\;167\;{\mu}g/mL$) showed good antioxidative effects. CWE and CWEA samples had dose-dependent inhibitory effects on the melanin production. The cytotoxic effects of the four CPWs were determined by colorimetric MTT assays using human keratinocyte HaCaT cells. Most extracts exhibited low cytotoxicity at $100\;{\mu}g/mL$. These results suggest CPWs are attractive candidates for topical applications on the human skin.

• KSBB Journal
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• 제32권1호
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• pp.9-15
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• 2017

Chamomile (Matricaria chamomilla), a member of the Asteraceae family, is a well-known for medicinal plant and can be found in India and Europe. Chamomile is an effective sedative and various medical effects. But, the effects of acne treatment by chamomile were not investigated. Therefore, we assessed the anti-oxidant effects, anti-microbial activity and anti-inflammatory effects by chamomile extracts in HaCaT keratinocyte cells. Anti-oxidant effects of chamomile extracts were investigated by DPPH assay. Also, results of MTT assay was demonstrated that chamomile extracts did not have a cytotoxic effect in HaCaT cells. To assess the antimicrobial activity, we determined formation of inhibition zone of Propionibacterium acnes by extracts from chamomile. Tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) induces production of inflammatory cytokines such as interleukin-$1{\beta}$ (IL-$1{\beta}$), IL-6 and IL-8 and expression of COX-2. Chamomile extracts could inhibit TNF-${\alpha}$-induced mRNA expression levels of IL-$1{\beta}$, IL-6, IL-8 and COX-2 gene. These results demonstrated the possibility of chamomile for prevention and treatment of skin inflammatory diseases such as acne.

• Journal of Microbiology and Biotechnology
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• 제32권11호
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• pp.1390-1395
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• 2022

Acne is a chronic inflammatory disease of the sebaceous gland attached to the hair follicles. Cutibacterium acnes is a major cause of inflammation caused by acne. It is well known that C. acnes secretes a lipolytic enzyme to break down lipids in sebum, and free fatty acids produced at this time accelerate the inflammatory reaction. There are several drugs used to treat acne; however, each one has various side effects. According to previous studies, sulforaphene (SFEN) has several functions associated with lipid metabolism, brain function, and antibacterial and anti-inflammatory activities. In this study, we examined the effects of SFEN on bacterial growth and inflammatory cytokine production induced by C. acnes. The results revealed that SFEN reduced the growth of C. acnes and inhibited proinflammatory cytokines in C. acnes-treated HaCaT keratinocytes through inhibiting NF-κB-related pathways. In addition, SFEN regulated the expression level of IL-1α, a representative pro-inflammatory cytokine expressed in co-cultured HaCaT keratinocytes and THP-1 monocytes induced by C. acnes. In conclusion, SFEN showed antibacterial activity against C. acnes and controlled the inflammatory response on keratinocytes and monocytes. This finding means that SFEN has potential as both a cosmetic material for acne prevention and a pharmaceutical material for acne treatment.

• 미생물학회지
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• 제55권3호
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• pp.213-219
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• 2019

여드름은 일반적인 피부 염증성 질환으로 알려져 있다. 여드름은 모낭 내 피지선에서 나타나는 만성 염증 질환이다. Propionibacterium acnes (P. acnes)의 증식은 대식세포가 염증성 사이토카인을 분비하도록 자극한다. 최근 연구에서 여러 천연 추출물이 P. acnes에 의해 매개되는 염증반응을 감소시키는 것을 확인하였다. 그러나 P. acnes에 의한 염증반응에서 E. persicifolia Gagnep. (E. persicifolia) 추출물의 억제효과에 관한 연구는 수행되지 않았다. 따라서 본 연구에서는 P. acnes에 의해 유도된 염증 반응에서 E. persicifolia 추출물의 항 염증효과를 조사하였다. P. persicifolia 추출물은 마우스 대식세포주인 RAW 264.7에서 P. acnes에 의해 유도된 IL-$1{\beta}$, IL-6, TNF-${\alpha}$ 및 iNOS와 같은 염증 매개체의 발현 수준을 억제하였다. 또한 E. persicifolia 추출물이 염증성 사이토카인 발현의 주요 조절인자인 NF-${\kappa}B$ 전사 활성화를 억제한다는 것을 발견했다. 본 연구 결과는 P. acnes의 치료를 위한 잠재적인 치료물질로서 E. persicifolia 추출물을 제안한다.

• 한국수산과학회지
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• 제54권1호
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• pp.111-117
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• 2021

The study was aimed at developing an alternative therapeutic agent against acne vulgaris, one of the most common skin diseases, to meet the continuing demand for new therapies. Acne vulgaris is often associated with the acne-causing bacteria such as Cutibacterium acnes. To investigate the safety of agents against acne vulgaris, we evaluated the potential antibacterial activities of edible seaweeds against C. acnes in Korea. Forty-one edible seaweeds, including the brown, green, and red varieties, were selected for the antibacterial test. In comparison with other seaweeds, 70% ethanolic extracts of brown seaweeds, such as Cladophora wrightiana var. minor, Eisenia bicyclis, Ecklonia cava, Ishige foliacea, Ishige okamurae, Sargassum filicinum, and Sargassum miyabei Yendo, exhibited potential antibacterial activity against C. acnes with minimum inhibitory concentrations ranging between 64 and 128 ㎍/mL. To investigate the active anti-acne agents and to enhance our understanding of the antibacterial activities against C. acnes, further solvent-fractionation experiments are warranted. The findings imply that brown seaweeds can be a potential source of natural agents against acne vulgaris.

• Journal of Applied Biological Chemistry
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• 제64권1호
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• pp.5-11
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• 2021

말오줌나무 잎 추출물의 생리활성 및 항균활성을 측정한 결과, 말오줌나무 잎 추출물의 생리활성은 말오줌나무 잎 추출물에 함유되어있는 phenolic성분들에 의해 효과가 발현되는 것으로 판단되었다. 말오줌나무 잎으로부터 생리활성에 관여하는 phenolic 화합물을 물과 에탄올로 추출하였을 때 각각 11.60±0.18과 12.39±0.07 mg/g의 함량을 나타내었다. 말오줌나무 잎의 ACE 저해 활성은 50-200 ㎍/mL의 phenolic을 함유한 물과 에탄올 추출물에서 각각 71.94-92.08와 48.42-78.33%의 저해 활성이 측정되었으며, 농도의존적인 양상을 나타내었다. 말오줌나무 잎 추출물의 XOase에 대한 추출물의 저해 활성은 50-200 ㎍/mL의 phenolic을 함유한 물과 에탄올 추출물에서 각각 42.11-100와 35.61-100%의 매우 우수한 저해효과를 나타내었다. 말오줌나무 잎 추출물에 대한 HAase 저해활성은 200 ㎍/mL의 phenolic을 함유한 농도에서 물과 에탄올 추출물이 각각 25.35±0.41와 43.38±2.26%의 HAase 저해활성을 나타내었다. 말오줌나무 잎 추출물의 항균활성은 충치균인 S. mutans와 식중독균인 S. aureus 및 여드름균인 P. acnes에 대한 우수한 항균 효과를 나타내었다. 따라서 말오줌나무는 고혈압억제, 통풍억제 및 염증억제 작용 등의 생리활성 및 S. mutans와 S. aureus 및 P. acnes에 대한 항균활성이 우수한 것으로 확인되어 말오줌나무를 활용한 기능성 식품 소재로서 산업화에 활용할 수 있을 것으로 생각되었다.

• Journal of Applied Biological Chemistry
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• 제63권4호
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• pp.443-449
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• 2020

본 연구에서는 섬괴불나무 잎으로부터 phenol성 화합물의 생리활성 탐색을 통하여 기능성 소재로 활용 가능성을 살펴보았다. 인체에 무해한 용매인 열수와 ethanol로 추출 했을 때 열수는 3.53 mg/g, 40% ethanol은 2.82 mg/g의 TPC를 나타내었으며, 추출물을 이용하여 항산화 활성, xanthine oxidase (XOase), angiotensin converting enzyme (ACE), α-glucosidase 억제효과 및 항균 활성을 검정하였다. 그 결과 섬괴불나무 잎의 열수와 ethanol 추출물 50-200 ㎍/mL TPC에서 농도의존적으로 DPPH 유리라디칼 소거활성 및 PF가 증가하는 경향을 보였고, 50 ㎍/mL의 저농도에서도 모두 매우 높은 활성을 나타내었다. XOase 저해효과에서는 200 ㎍/mL TPC에서 열수와 ethanol 추출물이 각각 76.35, 99.83%의 높은 저해효과 나타냈으며, ACE 저해효과 또한 200 ㎍/mL TPC에서 열수와 ethanol 추출물 각각 79.06, 87.14%의 저해효과를 확인하였다. α-Glucosidase 저해효과를 측정한 결과 200 ㎍/mL TPC에서 열수와 ethanol 추출물 각각 80.45, 63.58%의 저해효과를 보였다. 생육저해환으로 항균활성을 측정 결과 열수 추출물 200 ㎍/100 μL TPC에서 H. pylori, P. acne균에 대해 각각 11.5, 18.5 mm의 clear zone을 형성 하였고, ethanol 추출물 200 ㎍/100 μL 농도에서는 P. acne균에 대해 10 mm의 clear zone을 형성하였다. 따라서 본 연구를 통하여 섬괴불나무 잎 추출물의 항산화 활성, xanthine oxidase (XOase), angiotensin converting enzyme (ACE), α-glucosidase 억제효과 및 항균 활성이 있다고 판단되었다.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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• pp.243-244
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• 2003

The effects of Lactic acid bacteria have been investigated on anti-tumor. cholesterol reduction in blood. promotion of immune and skin-beauty. We are focused on cosmeceutical activity of Lactic acid bacteria (LAB). Ml, which is found in Korean traditional food. Kimchi The LAB.Ml has been identified as Lactobacillus plantarum Ml and individually cultured with Soybean soup and Soybean-Curd whey, until the total acidity has been reached the highest. After then, cell-free extracts from Ml have been used for the following studies. We assessed the effect of Lactobacillus plantarum Ml on the depigmentation of B16FlO melanoma cell. The melanin content of cells was decreased with 1-3% of cultured extracts. The tyrosinase activity was reduced by cell-free extracts of Lactobacillus plantarum Ml. Anti-aging and anti-oxidative activity of Ml cultured extract was also studied in NIH-3T3 human fibroblast cells. It showed that induction of cell proliferation. collagen synthesis and free radical scavenging activity. Additional studies for anti-fungal and anti-acne activity were also detected on Staphylococcus aureus and Propionibacterium acnes, respectively. These results suggest that cultured extract of Lactobacillun plantarum Ml would be used for cosmeceutical ingredients through multifunctional reaction on skin such as whitening, anti-wrinkle. anti-oxidation and anti-acnes.

• 한방안이비인후피부과학회지
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• 제26권1호
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• pp.50-62
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• 2013

Objective : Propionibacterium acnes (P. acnes) is a major pathogenic bacteria for acne vulgaris. This study was performed to evaluate the effects of Lithospermum erythrorhizon extracts on the inflammatory cytokines gene expression by P. acnes in human keratinocytes, HaCaT cell line. Methods : Anti-bacterial activity and cytotoxicity of LE extracts was analyzed by agar plate culture and XTT assay. The cytokines gene expressions were assessed by real time RT-PCR for IL-8, MCP-1 and TNF-${\alpha}$. During the cell culture and treatments, amounts of secreted TNF-${\alpha}$ were measured by ELISA. Translocation of transcription factor NF-${\kappa}B$ from cytoplasm into nucleus was observed by immunocytochemistry and confocal microscopy. Results : There were no anti-bacterial effects and cytotoxicity as high as $1,000{\mu}g/ml$ of LE extracts in XTT assay. Transcription levels of inflammatory cytokines, IL-8, MCP-1 and TNF-${\alpha}$ were increased by P. acnes in HaCaT. LE extracts decreased the upregulated gene transcription levels. However, amounts of secreted TNF-${\alpha}$ were similar in HaCaT cells with P. acnes and LE extracts. Translocation of NF-${\kappa}B$ into nucleus by P. acnes was significantly inhibited by LE extracts. Conclusions : From the results of this study, LE extracts have anti-inflammatory effects on HaCaT cells by P. acnes that decreased the mRNA expressions of IL-8, MCP-1 and TNF-${\alpha}$. This anti-inflammatory effects of LE extracts could provide the potential of therapeutic substance for acne vulgaris.