• Journal of the Korean Society of Tobacco Science
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• v.4 no.2
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• pp.31-36
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• 1982

The field evaluation were conducted on dihaploid progenies derived from anther culture of F1 plant of Drama x Sohyang (N. tabacum L.) The mean values of agronomic and chemical traits of dihaploids such as Nicotine, length/width, plant height, Leaf length, leaves per plant and Yield were greater than those of tar parents. The correlation coefficient of dihaploids were similar to those of conventional lines and varieties. In path analysis, in relating yield components to yield, leaves per plant and leaf width had a direct effect on Yield, but the other components such as plant height, leaf length and days to flowering influenced Yield indirect way.

• Plant Resources
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• v.1 no.2
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• pp.109-112
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• 1998

Aloe in vitro culture was attempted to induce callus and regeneration ability from different explant sources onto MS medium with 0.5mg/l NAA plus 1.0mg/l BA. Anthers that no developed any callus and plant regeneration, while only four out of 274 filament explants induced calli at cut edge without regenerated plants. Twenty ovary explants regenerated four direct plantlets without via callus from the base of epidermal tissues. Regenerated plants on the root tip gave 2n=14 of chromosome numbers.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.8 no.1
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• pp.117-119
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• 1970

The anthers of Yellow Special A, a leading tobacco variety, were cultured on the modified RM-1964 medium. Approximately three weeks after culture, plantlets emerged out of the anthers, and they reached blooming stage in six months. 24 univalent chromosomes were most frequently observed at M1 of PMC. Number of bivalent was 0.8 per PMC.

• Journal of the Korean Society of Clothing and Textiles
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• v.29 no.3_4 s.141
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• pp.569-575
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• 2005

The objective of this study is to consider the concept of postmodernism and the characteristics concerning the stage of the development, and to investigate the effect of the postmodernism on the clothing. Postmodernism is the result of the late capitalism and its characteristics are the narrative, mixture and deconstruction. First, since it is impossible to express the social culture of various society through one theoretical disclosure, the novel value on the narrative is provided and its characteristics arise. Hence, the society becomes afford to accept the other and provides the novel value to the absolute sub-concept. Second, the recognization and emphasis of the narrative in the post modem society bring about the repulsion as to the strict classification between the high culture and low one established from modernism and the closure between each genre of the art, and this causes to raise the composed characteristics which borrow or mix the style and image of different age and culture. Third, the deconstructive feature arises which eliminates the boundary between the high and low class, the past and future and the destruction and construction. This means not only anther recomposition but also skeptical attitude about it.

• Korean Journal of Plant Tissue Culture
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• v.22 no.1
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• pp.35-39
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• 1995

In order to investigate the effects of gelling agent on rice anther culture, anthers of rice (Japonica cv Daecheongbyeo) were cultured on N$_{6}$ media supplemented with 0.8, 1.2 or 1.6% Junsei agar and 05, 0.4, 0.6, 0.8 or 1.0% Gelrite (Phytagel, Sigma). On Junsei agar media, the frequency of callus induction was decreased in proportion to agar concentration. The frequency of callus induction was more increased as 67.6% and 54.8% in media containing 0.4 and 0.6% Gelrite than in agar media. The frequency of plant regeneration and spontaneous doubled-diploid was directly proportional to Junsei agar and Gelrite concentration. The number of green and spontaneous doubled diploid plant was highest on 0.6% Gelrite medium. In order to optimize the concentration of growth regulators for the callus induction medium containing 0.6% Gelrite, anthers were cultured on N$_{6}$ media supplemented with 2mg/L NAA, 2 mg/L 2,4-D, 1mg/L NAA and 1mg/L 2, 4-D, or 1mg/L NAA, 1mg/L 2,4-D and 0.5mg/L kinetin. The maximum frequency of callus induction and plant regeneration was obtained from the medium supplemented with 2 mg/L NAA and 0.6% Gelrite. In conclusion the induction of embryogenic callus, the frequency of plant regeneration and in vivo chromosome doubling was more effective in Gelrite media than in Junsei agar media.dia.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.4
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• pp.425-430
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• 1983

Rice (Oryza sativa L.) anthers collected from different genotypes were cultured to investigate the priority to increase callus production rate among several factors affecting on callus formation. Rice varieties, F$_1$ hybrids and F$_2$ plants differed greatly in their abilities to produce callus from anthers, and it was confirmed that the culturability of rice anther was a heritable characteristic. Cold shock treatment before plating anthers promoted callus formation rate, but combined effect with genotype having high culturability was more significant. The response to sucrose concentration in culture medium in callus induction rate was different between rice genotypes, but combined effect with genotype was not significant. Single supplement of NAA to R$_2$ medium increased callus production rate remarkably only in rice genotype having high culturability. Conclusively selection of genotypes is most important to increase callus initiation frequency from rice anthers.

• Journal of the Korean Society of Tobacco Science
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• v.14 no.1
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• pp.24-32
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• 1992

The present study was conducted to compare the relative efficiency of two different haploid breeding methods in tobacco varietal development. A single F t hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L., Bright Yellow4(BY4) and NC 95, was used to develop the 30 dihaploid lines by anther culture(F1-ADH) and maternally-derived doubled haploid utilizing Nicotiana africana(F1-MDH), respectively. As compared with mid-parent, ADH lines showed increasing in number of leaves, delaying in days to flower and narrowing in leaf width. However, no significant differences in the other characters investigated were recognized. MDH lines also showed narrow leaf width, while no significant differences in the other characters were observed. The variations of the characters investigated were generally greater in ADH than MDH lines. MDH lines had higher plant height and shorter days to flower than ADH lines, while other characters did not show remarkable differences. The degree of heritability for each of the characters observed between ADH and MDH was almost the same. The characters showing high heritability value were plant height, leaf number, days to flower, and yield, while those showing relatively low value were leaf length, leaf width, and total alkaloid content. Predicted gains from selection for increased yield were calculated for both populations(F1-ADH, F1-MDH) and correlated responses associated with selection for yield were estimated. Plant height, leaf width, days to flower, percent reducing sugar and disease resistance would be expected to improve with selection for yield much faster in the MDH population than in the ADH.

• Journal of the Korean Society of Tobacco Science
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• v.14 no.2
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• pp.104-115
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• 1992

The present study was conducted to compare the relative efficiency of four different breeding methods in tobacco varietal development. A single Fl hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L Bright Yellow 4(BY4) and NC95, was selfed. F2population above cross was screened for resistance against bacterial wilt caused by Pseudommonas solanacearum E.F.Smith under the naturally infested field conditions, and the 30 lines were developed from F2 individual plant by anther culture (ADH), maternal method utilizing Nicotiana afpicana (MDH), single- seed descent(SSD) and Bulk breeding method, respectively. All characters except content of total alkaloids of ADH and MDH which wore bred by haploid methods reduced more than that of lines bred by conventional methods(SSD & Bulk) : however, the yields were 8% lower than other lines. The total alkaloid content of ADH was higher than that of MDH, and yield was reduced about 4n even though the number of leaf was identical with the MDH. All other characters of ADH were also reduced significantly. In the lines bred by conventional methods, population developed by SSD showed significantly wider leaf width, shorter plant height, later days to flower, and lower in percent reducing sugar than those by Bulk. The populations derived from haploid method showed greater phenotypic variance and wider range of variation than conventionally developed ones. The results obtained indicate that selection will provide a significantly greater genetic gain for leaf number and leaf length in the ADU and MDH populations, and for plant height and days to flower in the SSD and Bulk populations.

• Korean Journal of Agricultural Science
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• v.4 no.2
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• pp.239-253
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• 1977

In order to establish the effective method of producing calluses of Prunus persica, anthers were cultured on Nitsch's medium supplemented with combinations of several growth regulators. Anthers of tetrad stage were preserved in the refrigerator at $4^{\circ}C$ for 50~60 days. Calluses were embeded on the Murashige and Skoog's medium supplemented with multiplicate and differentiate the calluses. Changes of anther color, callus formation, and proliferation of haploid callus were observed under the different medium conditions. The results obtained were summarized as fellows: 1) The cultured anthers were turned dark brown 2~6days after were explanted anthers into the medium. 2) The anthers which were not changed in color were observed more frequently in the medium not added the growth regulators. 3) Calluses were induced from anthers which were turned dark brown and liberated from the anther slit. 4) BA. was very effective to induce calluses and to form the chlorophyll. The medium supplied with BA 0.5ppm were best to induce calluses. 5) The best medium supplied with BA 0.5ppm+IAA or 2.4-D were best to proliferation of calluses. 6) The medium was adjusted to pH 4.5 and supplied with 250mg/l of $CaCl_2{\cdot}2H_2O$ were induced calluses from anthers.

• Journal of Korean Society of Forest Science
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• v.43 no.1
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• pp.6-13
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• 1979

In order to substract the time and cost of propagation for inducing the haploid plants per each species. 500 anthers of late uninucleate microspore on early binucleate microspore stage of Robinia pseudoacacia (Fuel tree) Punius granatum (Ornamental tree). Aleurites fordii (Faty tree) and Styrax japonica (Silvicultural tree) were cultured on the modified Murashige and Skoog's medium supplemented with Kinetin, 2.4-D and NAA as growth regulators. And I observed the samples of cultured anthers under the microscope which were made by Microtoming method and Paraffin method. The results were summarized as follows: 1) Among 500 cultured anthers per each species, anther numbers inducing the diploid callus were as follow: Styrax japonica 20 (4% for the species total); Aleurites fordii 10 (2% for the species, total) and Punica granatum 45 (9% for the species total) were showed. 2) 2n Callus were induced from anther wall. but haploid callus were induced from anther locule. 3) Haploid callus were induced only in 25 anthers (5% for the species total) of Robinia pseudoacacia. 4) These haploid callus were not originated from body cell of anther wall tissue, but from reduced microspores, 5) Since already reported many herbaceous haploid plants were induced from the callus which were originated from reduced microspores, I conclude that the anther of woody plant which induced the haploid callus also will be cultured haploid plant.