• Korean Journal of Veterinary Service
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• v.15 no.1
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• pp.17-25
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• 1992

For evaluation on effects guiding fasciola prevention of which the specimens collected from 6,011 cows rears at 387 farms in inchon were tested to egg count examination for 4 years(1987-1990), and the questionnaire checked up 181 dairy farms for 2 years (1989-1990). The results were summarized as follows. 1. A yearly variation of fasciola infection rate above 31% were 83% In 1987, 58% in 1988, 25% in 1989, noninfection in 1990. 2. The average concentration in feces of infected dairy cattle was 79.4% below 1-10 eggs for 4 years(1987-1990) 3. In the results of the questionnaire for 2 years(1989-1990) the farmers gradually took interest in fasciola prevention. 4. To re-dose anthelmintics was 75.4% in 1989 and 82.5% in 1990 after 5∼7 months from previous dosage.

• Korean Journal of Veterinary Research
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• v.38 no.4
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• pp.737-744
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• 1998

The rhizomes of the Acorus gramineus Soland have been used as sedatives, analgesics, stomachics and anthelmintics in chinese medicine. It is known that the rhizomes of the Acorus gramineus Soland contains the essential oil about 0.5~0.8% and this essential oil contains asarone about 86%. The asarone possess many pharmacological properties similar to those of reserpine and chlorpromazine. Sedative and analgesic effects of essential oil of Acorus gramineus Solaad in mouse was observed. The essential oil of Acorus gramineus Soland decreased the frequency of ambulation on mouse in proportion of concentration. ${\alpha}_2$ receptor antagonist(yohimbine hydrochloride) and opioids receptor antagonist(naloxone hydrochloride) were markedly decreased in frequency of ambulation. The essential oil of Acorus gramineus Soland decreased writhing syndrome in mouse and ${\alpha}_2$ receptor antagonist(yohimbine hydrochloride), opioids receptor antagonist(naloxone hydrochloride) were not increased above effects. In conclusion, these experimental results show that the essential oil of Acorus gramineus Soland have sedative and analgesic effects, but it did not antagonized ${\alpha}_2$ receptor antagonist(yohimbine hydrochloride) and opioids receptor antagonist(naloxone hydrochloride).

• Korean Journal of Veterinary Research
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• v.58 no.3
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• pp.167-170
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• 2018

Two 12-month-old cattle with anthelmintics containing trichlorfon the day before death presented to the Animal and Plant Quarantine Agency for diagnosis. In necropsy, they revealed enlargement of the spleens, redness of mucosa and serosa in stomachs and intestines, and friable kidneys. Histopathologically, hemorrhages in the spleens, omasums, abomasums, and intestines as well as renal tubular necrosis were observed. Trichlorfon was detected at above the lethal dose in the ruminal contents. Based on these findings, we diagnosed this case as death caused by trichlorfon poisoning.

• Korean Journal of Veterinary Research
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• v.24 no.1
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• pp.83-90
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• 1984

In the evaluation of anthelmintic efficacy by fecal egg counts for cattle naturally infected with helminths, the reasonable technique of fecal egg counts and the reliable guidelines were determined as follows; 1. Modified technique of Harashigeru and Kim's sedimental tube was the most reasonable in fluke egg counts. 2. Universal egg counting technique was preciser than McMaster egg counting technique and was lower in coefficient variation. 3. Fecal egg counts of pretreatment should be carried out twice and mean of the epg should be calculated. 4. Fecal egg counts of posttreatment should be carried out 3 times and established at suitable intervals in consideration of anthelmintic mechanism and withdrawal days of anthelmintics. 5. If nematodal eggs are not found by UECT in posttreatment, direct flotation method should be carried out. And if positive, this epg was calculated at 20 (factor of UECT; $40{\times}1/2$).

• Korean Journal of Veterinary Service
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• v.44 no.3
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• pp.125-131
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• 2021

Benzimidazole is an widely used anthelmintics in livestock clinics. The aim of present study was to investigate benzimidazole residues in the meat in Sejong City. A total of 338 samples of meat(beef 287, pork 37, chicken 14) were selected from the laboratory meat(Korean beef genetic test, hygiene test) supplied to school in Sejong City. Representative benzimidazole class drug such as thiabendazole, 2-amino albendazole sulfone, fenbendazole, oxfendazole, and oxfendazole sulfone were monitored in samples by using lc-ms/ms and the analysis was preformed based on the Korea Food Code guideline. Limit of quantitation (LOQ) was ranged from 0.56 to 3.02 ppb for target drugs and the accuracy was within the acceptance criteria. Among the sample analyzed only one case, the pork meat was found to have drug levels that exceed LOQ. However, the overall residue concentration was 2.17 ppb, which was lower than the maximum residue levels (100 ppb).

• Parasites, Hosts and Diseases
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• v.30 no.3
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• pp.163-168
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• 1992

Hugot (1983), Hugot and Tourte-Schaefer (1985) reported that nematodes of the genus nnterobius causing human enterobiasis might be classified into two difEerent species on the basis of the size and shape of spicule in male worms. E. gregorii Hugot, 1983 has signif;cantly shorter spicules in males than those in E. vermicularis. A total of 194 male Enterobius were collected from 17 schoolchildren by anthelmintics at Wonju, Kangwon-do. The sizes of spicules and morphologies were comparatively observed. Two different types of spicules in male Enterobius were also observed in Korea. Out of 112 male worms observed, the percentages of E. gregorii (short-spicule type) and E. vermicularis (long-spicule type) were 23.2% and 76.8%, respectively.

• Korean Journal of Veterinary Service
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• v.28 no.1
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• pp.81-89
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• 2005

Recently, mass spectrometry coupled with liquid chromatography (LC/MS) has been a preferred technique for determination of organic compounds in complex matrixes. LC/MS provides a high degree sensitivity and specificity of the compounds of interest. The purpose of this study was to confirm analytical method of residual 6 benzimidazoles (thiabendazole, oxfendazole, mebendazole, albendazole, flubendazole and fenbendazole) in meat by LC/MS. Benzimidazoles were analyzed by LC/MS on XTerra $C_{18}$ column with 0.01% trifluoroacetic acid-acetonitrile (TFA) in a gradient mode as mobile phase, and that were identified by electrospray ionization with selected ion recording mode at 150-350 amu mass range. Residual benzimidazoles were extracted from tissue with ethylacetate, and elute benzimidazoles with $50\%$ acetonitrile. In the LC/MS analysis of benzimidazoles, signal to noise ratio was showed relatively high in the positive mode and special ion in the quality analysis was determined via $[M+H]^+$ and Fragment ions. A spectrum of benzimidazoles was showed from all 6 benzimidazoles

• Korean Journal of Veterinary Research
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• v.38 no.3
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• pp.589-594
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• 1998

The in vitro screening tests against the in vitro cultivated $L_3$ of Ascaris suum (in vitro $L_3$), which were cultivated from the embryonated egg to third-stage larva on 7 days in culture(DIC) and the in vivo rat's lung-derived $L_3$ of Ascaris suum (in vivo $L_3$), which were recovered from the lungs of rat on 7 days after infection, carried out in order to compare the anthelmintic efficacy of in vitro $L_3$ and that of in vivo $L_3$ in RPMI medium 1640 with 5% bovine calf serum. And also a screening test of efficacy against adult worms of Trichuris suis performed. The efficacies of screening tests were as follows : 1. The screening efficacies of abamectin and ivermectin against the in vitro $L_3$ were all 100% at the 10ppm concentration in RPMI medium 1640 on 5 DIC. 2. The screening efficacies of abamectin and ivermectin against the in vivo $L_3$ were all 100% at the 20ppm on 5 DIC or at 40ppm on 3 DIC. 3. The screening efficacies of abamectin and ivermectin against the adult worms of Trichuris suis were all 100% at 20ppm on 4 DIC. And therefore, the in vitro cultivated $L_3$ of Ascaris suum were used in the screening test as well as the in vivo rat's lung-derived $L_3$ of Ascaris suum. And also the adult worms such as Trichuris suis and filaroids which is small size and difficult to cultivate to vitro, were used in the screening test in vitro.

• Parasites, Hosts and Diseases
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• v.30 no.1
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• pp.59-62
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• 1992

An imported case of rectal hookworm infection was diagnosed by stool examination and recovery of adult worms from the rectal mucosa by sigmoidoscopy. The chief complaints of a patient were diarrhea, abdominal pain and weight loss for about 1 month after returning from his travel abroad to the Southeast Asia. Leukocytosis ($16, 750/{\mu}\ell$) and peripheral eosinophilia (33.7%) were noticed without anemia. Typical hookworm eggs were detected by stool examination, and 3 worms were collected by sigmoidoscopy from rectal mucosa of this patient. Those worms were confirmed as adult worms of Ancylostoma duodenale(♂:1, ♀: 2) based on their morphological characteristics. The symptoms were relieved after treatment with anthelmintics. This case was considered as one of the imported parasitic infections in Korea, and a rare case of hookworm infection on human rectal mucosa.

• Parasites, Hosts and Diseases
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• v.51 no.3
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• pp.297-304
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• 2013

Trichinosis is a parasitic zoonosis caused by the nematode Trichinella spiralis. Anthelmintics are used to eliminate intestinal adults as well as tissue-migrating and encysted larvae. This study aimed to investigate the effects of ivermectin and myrrh obtained from the aloe-gum resin of Commiphora molmol on experimental trichinosis. Ninety albino mice were orally infected with 300 T. spiralis larvae. Drugs were tested against adult worms at day 0 and day 5 and against encysted larvae on day 15 and day 35 post-infection (PI). Mature worms and encysted larvae were counted in addition to histopathological examination of muscle specimens. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), total protein, albumin, globulin, urea, and creatinine values were estimated. Significant reductions in mean worm numbers were detected in ivermectin treated mice at day 0 and day 5 PI achieving efficacies of 98.5% and 80.0%, while efficacies of myrrh in treated mice were 80.7% and 51.5%, respectively. At days 15 and 35 post-infection, ivermectin induced significant reduction in encysted larval counts achieving efficacies of 76.5% and 54.0%, respectively, while myrrh efficacies were 76.6% and 35.0%, respectively. AST, ALT, urea, and creatinine levels were reduced, while total proteins were increased in response to both treatments compared to their values in the infected non-treated mice. Ivermectin use for controlling T. spiralis could be continued. Myrrh was effective and could be a promising drug against the Egyptian strains of T. spiralis with results nearly comparable to ivermectin.