• 한국식품위생안전성학회지
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• 제35권6호
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• pp.535-543
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• 2020

유전적 조절, 유전자 발현 그리고 환경적 단서, 화학적 신호에 대응하는 표현형 변이에서 세포 RNA는 ubiquitous 역할 이외에도 세포 외 RNA(exRNA)라 하는 새로운 형태의 RNA는 추후 연구의 방향을 제시한다. exRNA는 membrane vesicles 또는 세포 외 소포체(EV)로 알려진 membrane blebs를 통해 세포 외부로 운반된다. EV의 형성은 원핵생물, 진핵생물, 고세균을 포함한 모든 미생물군에 우세하게 보존되어있다. 본 리뷰는 세균 유래 exRNA에 관해 세가지 주제에 초점을 두었다. exRNA의 발견과 박테리아 유전자 배열에 대한 외부 RNA의 영향, b. exRNA의 분비기작을 통한 방출, c. 다른 그람음성 및 그람양성균에 의해 분비되는 exRNA로 고안될 수 있는 응용 가능분야이다. 본 리뷰에서 장내 미생물군의 probiotics 및 후성유전학적 규제에서 본 exRNA와 exRNA마커와 같은 EV파생 응용프로그램에 대한 의견을 제공할 것이다.

• International Journal of Computer Science & Network Security
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• 제22권1호
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• pp.358-366
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• 2022

The article considers current information technologies in the organization of educational work in secondary schools in Great Britain. The forms and types of organization of educational work in secondary schools of Great Britain with the help of modern information technologies are covered. The purposes of education with the help of modern information technologies are singled out. Mental, physical, labor, moral, aesthetic and other types of education, merged in a holistic educational process, provide an opportunity to achieve the main goal - the formation of a comprehensive and harmoniously developed personality. All forms of educational work have their pedagogical significance, and each of them is valuable in the process of education. Democratization and partnership development are the leading principles of education in British schools at the present stage; multiculturalism and student independence. This is the ability to promote the role of different types of education, such as: labor, moral, legal, religious, family, environmental, aesthetic, civic, physical, mental education, which is now possible with the help of current information technology. The article considers current information technologies in the organization of educational work in secondary schools in Great Britain. The forms and types of organization of educational work in secondary schools of Great Britain with the help of modern information technologies are covered. The purposes of education with the help of modern information technologies are singled out. Mental, physical, labor, moral, aesthetic and other types of education, merged in a holistic educational process, provide an opportunity to achieve the main goal - the formation of a comprehensive and harmoniously developed personality. All forms of educational work have their pedagogical significance, and each of them is valuable in the process of education. Democratization and partnership development are the leading principles of education in British schools at the present stage; multiculturalism and student independence. This is the ability to promote the role of different types of education, such as: labor, moral, legal, religious, family, environmental, aesthetic, civic, physical, mental education, which is now possible with the help of current information technology.

• 대한본초학회지
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• 제34권6호
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• pp.79-89
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• 2019

Objective : This study investigated the anti-diabetic effects of DM1, a herbal mixture with Atractylodis Rhizoma, Anemarrhenae Rhizoma, and Cinnamomi Cortex in high fat diet (HFD)-induced diabetic mice and the mechanism in C2C12 mouse skeletal muscle cells. Methods : The C57B/6 mice were fed high fat for 12 weeks, and then administrated DM1 extract (500 mg/kg, p.o.) for 4 weeks. The changes of body weight, calorie and water intakes, fasting blood glucose levels and the serum levels of glucose, insulin, triglyceride, HDL-cholesterol, AST and ALT were measured in mice. The histological changes of liver and pancreas tissues were also observed by H&E stain. C2C12 myoblasts were differentiated into myotubes and then treated with DM1 extract (0.5, 1, and 2 mg/㎖) for 24 hr. The expression of myosin heavy chain (MHC), PGC1α, Sirt1 and NRF1, and the AMPK phosphorylation were determined in the myotubes by western blot, respectively. Results : The DM1 extract administration significantly decreased the calorie and water intakes, glucose, triglyceride, AST and ALT levels and increased insulin and HDL-cholesterol in HFD-induced diabetic mice. DM1 extract inhibited lipid accumulation in liver tissue and improved glucose tolerance. In C2C12 myotubes, DM1 treatment increased the expression of MHC, PGC1α, Sirt-1, NRF-1 and the AMPK phosphorylation. Conclusion : In our results indicate that DM1 can improve diabetic symptoms by decreasing the obesity, glucose tolerance and fatty liver in HFD-induced diabetic mice, and responsible mechanism is might be related with energy enhancement.

• 예술경영연구
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• 제56호
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• pp.5-38
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• 2020

본 연구는 문화기반 마을공동체가 어떻게 고유의 공동체 문화를 형성하고, 지속가능한 성숙한 공동체가 되는지 파악하기 위해 정책대상자의 인식, 정서, 역량에 대해 살펴보았다. 공동체의 개념, 공동체 성장요인 및 성장단계, 문화활동, 그리고 정책 집행이론에 대한 선행연구를 살펴보고, 문화기반 마을공동체 사업에 적합한 정책집행 분석모형을 도출하였다. 연구방법은 질적사례연구방법을 채택하였고, 연구대상은 안산 사동 '감골주민회', 안산 대부동 '상동공동체', 안동 신세동 '그림애문화마을'로 문화기반 마을공동체의 성공사례를 선정하였다. 심층인터뷰와 문헌분석, 직접관찰을 통해 얻은 자료를 로버트 인(2009)이 제시한 패턴매칭, 사례통합 분석기법을 활용하여 분석하였다. 연구결과는 첫째, 정책대상자는 공동체 활동을 통해 신뢰와 정서적 친밀감, 소속감을 느끼는 정서적 변화를 겪으며, 지역에 대한 애착심이 향상되었다. 둘째, 정책대상자의 공동체 의식과 공동체 역량이 성장에 중요한 요인으로 나타났다. 공동체 활동을 통해 참여유도, 상호교류, 역량강화, 파트너쉽의 단계를 거치며 성장해나가고 있음을 알 수 있었다. 셋째, 다양한 창의적 문화활동을 통해 스스로를 표현하면서 자신을 재발견하고, 타인과 소통하면서 마을공동체의 공통적 고민과 사회적 문제를 해결하는 경험을 하는 것으로 나타났다. 본 연구는 문화기반 마을공동체에 대한 생태학적 접근 연구를 기반으로 정책집행 분석 모형을 도출하였고, 문화기반 마을공동체의 사례를 분석하여 공동체의 성장 방향성과 실천적 함의를 제시하였다.

• International Journal of Computer Science & Network Security
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• 제22권10호
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• pp.17-24
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• 2022

The article proves that the main goal of informatization of higher education institutions in the EU countries is to improve the quality of education of future specialists by introducing digital educational resources into the education process. The main tasks of informatization of education are defined. Digital educational resources are interpreted as a set of data in digital form that is applicable for use in the learning process; it is an information source containing graphic, text, digital, speech, music, video, photo and other information aimed at implementing the goals and objectives of modern education; educational resources on the Internet, electronic textbooks, educational programs, electronic libraries, etc. The creation of digital educational resources is defined as one of the main directions of informatization of all forms and levels of Education. Types of digital educational resources by educational functions are considered. The factors that determine the effectiveness of using digital educational resources in the educational process are identified. The use of digital educational resources in the training of future specialists in the EU countries is considered in detail. European countries note that digital educational resources in professional use allow you to implement a fundamentally new approach to teaching and education, which is based on broad communication, free exchange of opinions, ideas, information of participants in a joint project, on a completely natural desire to learn new things, expand their horizons; is based on real research methods (scientific or creative laboratories), allowing you to learn the laws of nature, the basics of techniques, technology, social phenomena in their dynamics, in the process of solving vital problems, features of various types of creativity in the process of joint activities of a group of participants; promotes the acquisition by teachers of various related skills that can be very useful in their professional activities, including the skills of using computer equipment and various digital technologies.

• Animal Bioscience
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• 제35권12호
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• pp.1827-1838
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• 2022

Objective: The semen quality of stallions including sperm motility is an important target of selection as it has a high level of individual variability. However, effects of the molecular architecture of the genome on the mechanisms of sperm formation and their preservation after thawing have been poorly investigated. Here, we conducted a genome-wide association study (GWAS) for the sperm motility of cryopreserved semen in stallions of various breeds. Methods: Semen samples were collected from the stallions of 23 horse breeds. The following semen characteristics were examined: progressive motility (PM), progressive motility after freezing (FPM), and the difference between PM and FPM. The respective DNA samples from these stallions were genotyped using Axiom Equine Genotyping Array. Results: We performed a GWAS search for single nucleotide polymorphism (SNP) markers and potential genes related to motility properties of frozen-thawed semen in the stallions of various breeds. As a result of the GWAS analysis, two SNP markers, rs1141327473 and rs1149048772, were identified that were associated with preservation of the frozen-thawed stallion sperm motility, the relevant putative candidate genes being NME/NM23 family member 8 (NME8), olfactory receptor family 2 subfamily AP member 1 (OR2AP1), and olfactory receptor family 6 subfamily C member 4 (OR6C4). Potential implications of effects of these genes on sperm motility are herein discussed. Conclusion: The GWAS results enabled us to localize novel SNPs and candidate genes for sperm motility in stallions. Implications of the study for horse breeding and genetics are a better understanding of genomic regions and candidate genes underlying stallion sperm quality, and improvement in horse reproduction and breeding techniques. The identified markers and genes for sperm cryotolerance and the respective genomic regions are promising candidates for further studying the biological processes in the formation and function of the stallion reproductive system.

• International Journal of Computer Science & Network Security
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• 제22권6호
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• pp.374-381
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• 2022

The article identifies and theoretically substantiates the trends of national resilience in the context of establishing the security of the country and its civilizational subjectivity. The strategy of development of the pedagogical university in the conditions of European integration into the European educational and scientific space based on certain characterological features of the personality of the volunteer in the context of allocation of personal resilience is developed. The analysis of both external and internal challenges and threats to the civilization of the country needs to be understood in the context of economic, socio-political, legal, military-political, spiritual-cultural, educational-scientific and network-information resilience. The concepts of "national resilience" and "national security" are quite close - at first glance, even identical. However, a deeper understanding clarifies the differences: national security is a state of protection of the country identity and its very existence, the realization of its national interests. In turn, resilience is a fairly effective strategy and a fundamental guarantee of national security. At the same time, it is extremely important to understand that both national security as a state and national resilience as a strategy are only means of achieving and developing a strong and humanistic civilizational subjectivity of the country. After all, such subjectivity opens for citizens the opportunity for development, dignified self-realization and a proper life. The restructuring of the volunteer's motivational sphere is due to the dominance of such leading motives, which are focused mainly on maintaining and restoring health, which leads to distorted meaningful life goals: isolation, alienation, passivity, inertia, reduced activity, limited communication, etc. The characteristics of relatively stable human behavior include several primary and secondary properties. The primary (relevant) properties include patience, trust, hope, faith, confidence, determination, perseverance, and love; the secondary - punctuality, neatness, obedience, honesty, loyalty, justice, diligence, thrift, accuracy, conscientiousness, obligation, etc. The restructuring of the volunteer's motivational sphere is due to the dominance of such leading motives, which are focused mainly on maintaining and restoring health, which leads to distorted meaningful life goals: isolation, alienation, passivity, inertia, reduced activity, limited communication, etc. The characteristics of relatively stable human behavior include several primary and secondary properties. The primary (relevant) properties include patience, trust, hope, faith, confidence, determination, perseverance, and love; the secondary - punctuality, neatness, obedience, honesty, loyalty, justice, diligence, thrift, accuracy, conscientiousness, obligation, etc. The use of information and communication technologies in volunteering will contribute to the formation of resilience traits in the structure of personality formation. Directly to the personal traits of resilience should be included methodological competencies, which include methodological knowledge, skills and abilities (ability to define ultimate and intermediate goals, plan, conduct and analyze knowledge, establish and implement interdisciplinary links with disciplines of medical-psychological-pedagogical cycles, etc.). All these competencies form the professional resilience of the volunteer.

• Nutrition Research and Practice
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• 제16권5호
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• pp.549-564
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• 2022

BACKGROUND/OBJECTIVES: Oxidative stress is caused by an imbalance between harmful free radicals and antioxidants. Long-term oxidative stress can lead to an "exhausted" status of antioxidant defense system triggering development of metabolic syndrome and chronic inflammation. Green perilla (Perilla frutescens) is commonly used in Asian cuisines and traditional medicine in southeast Asia. Green perilla possesses numerous beneficial effects including anti-inflammatory and antioxidant functions. To investigate the potentials of green perilla leaf extract (PE) on oxidative stress, we induced oxidative stress by high-fat diet (HFD) in aging mice. MATERIALS/METHODS: C57BL/6J male mice were fed HFD continuously for 53 weeks. Then, mice were divided into three groups for 12 weeks: a normal diet fed reference group (NDcon), high-fat diet fed group (HDcon), and high-fat diet PE treated group (HDPE, 400 mg/kg of body weight). Biochemical analyses of serum and liver tissues were performed to assess metabolic and inflammatory damage and oxidative status. Hepatic gene expression of oxidative stress and inflammation related enzymes were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: PE improved hepatopathology. PE also improved the lipid profiles and antioxidant enzymes, including hepatic glutathione peroxidase (GPx) and superoxide dismutase (SOD) and catalase (CAT) in serum and liver. Hepatic gene expressions of antioxidant and anti-inflammatory related enzymes, such as SOD-1, CAT, interleukin 4 (IL-4) and nuclear factor erythroid 2-related factor (Nrf2) were significantly enhanced by PE. PE also reduced the levels of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) in the serum and liver; moreover, PE suppressed hepatic gene expression involved in pro-inflammatory response; Cyclooxygenase-2 (COX-2), nitric oxide synthase (NOS), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6). CONCLUSIONS: This research opens opportunities for further investigations of PE as a functional food and possible anti-aging agent due to its attenuative effects against oxidative stress, resulting from HFD and aging in the future.

• 대한바이러스학회지
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• 제29권2호
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• pp.55-64
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• 1999

In Yugoslavia, hemorrhagic fever with renal syndrome (HFRS) is one of the important national health problem, but no vaccine has been used to prevent HFRS. Since first HFRS case in 1952, sporadic cases of HFRS occurred every year and over 4,000 registered cases with $1{\sim}16%$ mortality so far. We performed a prospective, randomized double-blind placebo-controlled trial to evaluate the effectiveness of $Hantavax^{TM}$ against HFRS in 3,900 healthy adults living in the endemic areas of Yugoslavia. 1,900 people were given 0.5 ml of Hantavax subcutaneously twice at one month interval and a booster shot at one year after. For controls other 2,000 healthy people were given 0.5 ml of physiolosical saline as a placebo. We investigated HFRS cases in both the vaccinated and nonvaccinated groups by monitoring the program for patient registration in the areas from 1996 to 1998, and the effect of vaccine was analyzed epidemiologically. No confirmed case of HFRS was observed among 1,900 Hantavax vaccinees, while 20 confirmed cases were observed among 2,000 nonvaccinated control group. There were no remarkable side effects among the vaccinees either locally or in general after inoculation of the vaccine. The Hantavax vaccine showed statistically significant protective efficacy against HFRS among Yugoslavian people.

• 한국심리학회지 : 문화 및 사회문제
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• 제21권3호
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• pp.511-534
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• 2015

본 연구의 목적은 캐나다 소재 대학교에 재학 중인 한국인 대학생을 대상으로 지각된 차별감과 우울의 관계에서 대처양식, 문화 정체성, 이중문화 자기효능감의 조절효과를 검증하는 것이다. 이를 위해 재캐나다 한국인 대학생 214명의 설문지 자료를 사용하여 위계적 회귀분석을 실시하였다. 연구의 결과는 다음과 같다. 첫째, 재캐나다 한국인 대학생들이 지각하는 차별감은 그들의 우울수준을 유의미하게 증가시켰다. 둘째, 대처양식의 세 유형인 사려형 대처, 억제형 대처, 반동형 대처 모두 지각된 차별감과 우울의 관계에서 유의미한 조절효과를 나타내지 않았다. 셋째, 지각된 차별감, 억제형 대처, 모국문화 정체성의 삼원 상호작용은 우울에 유의미한 영향을 미쳤다. 즉 모국문화 정체성이 낮은 경우 지각된 차별감의 수준이 높을 때, 억제형 대처를 많이 사용하면 우울의 수준도 상응해서 증가하나, 억제형 대처를 적게 사용하면 우울수준이 대폭 감소하는 것으로 나타났다. 넷째, 지각된 차별감과 사려형, 억제형, 반동형 대처의 각 유형과 이중문화 자기효능감의 우울에 미치는 삼원 상호작용 효과는 유의미하지 않았다. 논의에서 연구결과에 대한 이론적 논의와 제한점 및 제언을 진술하였다.