• Parasites, Hosts and Diseases
• /
• v.30 no.4
• /
• pp.263-268
• /
• 1992

A study was presented on the anisakid larvae in Astroconger myriaster which were caught at the Southern Sea and waled at Pusan area. The nematodes were morphologically classified into Anisakis sp. and Centracgecum sp. after fixation in 70% ethanol and clearing in lactophenol. Total of 1, 768 larval anisakids were collected from 259(67.8% positive rate) out of 382 examined A. myriaster. Total 642 larvae of Anisakis sp. were obtained from 94 (24.6%) and 1, 126 Ccntracaecum sp. were recovered from 165 (43.1%) fish. The average number of worms per infected fish was 4.6. The infection rate increased according to the length of fish, and all of the fish over 71 cm were found infected. The numbers (proportions) of recovered worms by the organs were 1, 440 (80.5%) in the omentuin, 166(9.4%) in the intestine, 107(6.0%) in the stomach, 32(1.8%) in the skin, 18(1.0%) in the muscle, 13(0.7%) in the liver, and 2(0.1%) in the head. The larvae in the muscle may infect the humans who are enjoying raw sliced meat of the fish.

• Parasites, Hosts and Diseases
• /
• v.52 no.1
• /
• pp.9-19
• /
• 2014

Anisakis spp. (Nematoda: Anisakidae) parasitize a wide range of marine animals, mammals serving as the definitive host and different fish species as intermediate or paratenic hosts. In this study, 18 fish species were investigated for Anisakis infection. Katsuwonus pelamis, Euthynnus affinis, Caranx sp., and Auxis thazard were infected with high prevalence of Anisakis type I, while Cephalopholis cyanostigma and Rastrelliger kanagurta revealed low prevalence. The mean intensity of Anisakis larvae in K. pelamis and A. thazard was 49.7 and 5.6, respectively. A total of 73 Anisakis type I larvae collected from K. pelamis and A. thazard were all identified as Anisakis typica by PCR-RFLP analysis. Five specimens of Anisakis from K. pelamis and 15 specimens from A. thazard were sequenced using ITS1-5.8S-ITS2 region and 6 specimens from A. thazard and 4 specimens from K. pelamis were sequenced in mtDNA cox2 region. Alignments of the samples in the ITS region showed 2 patterns of nucleotides. The first pattern (genotype) of Anisakis from A. thazard had 100% similarity with adult A. typica from dolphins from USA, whereas the second genotype from A. thazard and K. pelamis had 4 base pairs different in ITS1 region with adult A. typica from USA. In the mtDNA cox2 regions, Anisakis type I specimens from A. thazard and K. pelamis showed similarity range from 94% to 99% with A. typica AB517571/DQ116427. The difference of 4 bp nucleotides in ITS1 regions and divergence into 2 subgroups in mtDNA cox2 indicating the existence of A. typica sibling species in the Makassar Strait.

• The Korean Journal of Malacology
• /
• v.30 no.3
• /
• pp.295-298
• /
• 2014

Finless porpoise is the one of porpoises in Korean waters that frequently observed in the coastal area. This study reported parasite infection cases of stranded finless porpoise, Neophocaena asiaeorientalis which was stranded on the Saemangeum Dyke on the west coast of Korea 21 May, 2012. The finless porpoise was male, and estimated at 8 years old. Parasites were found in abdominal cavity, skull and lung. The parasites were identified as nematode, trematoda and lungworm. Nematoda in abdominal cavity was Crassicauda sp. Trematoda found in the skull was indentified Nasitrema sp. Lungworm in lung seemed one of genus Pseudaliids. Anisakis spp. has not been found.

• Parasites, Hosts and Diseases
• /
• v.31 no.4
• /
• pp.371-374
• /
• 1993

Fecal samples of cats purchased in Seoul were examined for helminth ova or protozoan oocysts from December 1987 to March 1988. Out of the 41 samples,31 (75.6%) were positive and 60 (146.3%) were cumulative positive for parasites. The followings were identified In the samples: Eggs of Toxocarn cacti. Clonorchis sinensis, Metoeonimn sp., Phnrvngostomum cordntum, Spirometra erinocei, Tcenia toenicejormis and oocysts of Isosporn sp. From nine autopsied cats, larvae of Anisakis simplex, adults of C. sinensis, M. yokogawai. P cordatum, S. erinacei and T. tqeniaejormis were identified. This is the first report on the detection of Anisakis larvae from cats In Korea. The possible role of cats as a source of human infection with each parasite was discussed. Key words: Cat, intestinal parasite, Anisckis simplex, Toxoccra cati, Clonorchis sinensis, Metagonimus yokogawai. Spirometro erinccei, Taenic tonniaelormis, Isospora Sp .

• Parasites, Hosts and Diseases
• /
• v.53 no.3
• /
• pp.349-353
• /
• 2015

Anisakis simplex sensu stricto (s.s.), Anisakis pegreffii, Anisakis berlandi (=A. simplex sp. C), and Anisakis typica are the 4 major species of Anisakis type I larvae. In the Republic of Korea (Korea), A. pegreffii, A. berlandi, and A. typica larvae in fish hosts has seldom been documented. In this study, molecular analysis was performed on Anisakis larvae from the sea eels (Astroconger myriaster), the major source of human anisakiasis in Korea, collected from Tongyeong City, a southern coastal area of Korea. All 20 sea eels examined were infected with Anisakis type I larvae (160 larvae; 8 per fish). Their species were analyzed using PCR-RFLP patterns and nucleotide sequences of internal transcribed spacers (ITS1, 5.8 subunit gene, and ITS2) and mitochondrial cytochrome c oxidase 2 (cox2). Most (86.8%; 112/129) of the Anisakis type I larvae were A. pegreffii, and 7.8% (10/129) were A. typica. The remaining 5.4% (7/129) was not identified. Thus, A. pegreffii is the major species of anisakid larvae in sea eels of the southern coast of Korea.

• Parasites, Hosts and Diseases
• /
• v.61 no.3
• /
• pp.240-250
• /
• 2023

The genus Anisakis is among the most significant parasites to public health, as it causes anisakiasis, a parasitic infection in humans resulting from consuming raw or undercooked seafood. Although the infection status of Anisakis in second intermediate hosts, such as marine fishes and cephalopods, and humans have been severally reported in Korea, no information about the definitive host in Korean waters is available. In 2014, 2 adult gastric nematodes were collected from a common minke whale (Balaenoptera acutorostrata) found in the East Sea, Korea. These worms were identified as A. simplex sensu stricto (s.s.) by comparing the mitochondrial COX2 marker with previously deposited sequences. Phylogenetic and phylogeographic analyses of A. simplex (s.s.) worldwide revealed 2 distinct populations: the Pacific population and the European waters population. This is the first report on adult Anisakis and its definitive host species in Korea. Further studies on Anisakis infection in other cetacean species and marine mammals in Korean seas are warranted.

• Applied Microscopy
• /
• v.41 no.3
• /
• pp.205-213
• /
• 2011

Numerous anisakids were parasitic near the digestive tract of the black rockfish, Sebastes schlegeli and some anisakids observed on the liver of the host. Anisakids in the host were identified three species (Hysterothylacium sp., Anisakis simplex, A. pegreffii) and Hysterothylacium sp. was the high occurrence of anisakid worms in the host. Hysterothylacium sp. was shorter and thinner than A. simplex. Both of anisakids observed lip, mouth, nerve ring, excretory pore and excretory duct in the anterior portion. In the mid portion, anisakids had esophagus, ventriculus and intestine and especially, Hysterothylacium sp. had intestinal ceacum and ventricular appendage. There was conical nodulose apex at the end of the posterior portion in Hysterothylacium sp. and spine in A. simplex. SEM examination revealed that there was three lip near the mouth of Hysterothylacium sp. Dorsal lip was approximately 65 ${\mu}m$ and paried lateroventral lip were approximately 60 ${\mu}m$ in the width. All lips were found double papilla (approximately 8 ${\mu}m$ in the width). The body width of the Hysterothylacium sp. and A. simplex was approximately 480 ${\mu}m$ and 900 ${\mu}m$ respectively. The hight of the lateral alae was about 7 ${\mu}m$ and width of papilla on the cornical nodulose apex was about 3.3 ${\mu}m$ in Hysterothylacium sp. The hight of spine was approximately 20 ${\mu}m$ in A. simplex. There was mainly Hysterothylacium sp. in the intestinal lumen of the host. The nematod worms were parasitic near the mucosal fold and in the submucosal. In the mucosal epidermal layer, it was increased mucous cells by the infection of the parasites.

• Fisheries and Aquatic Sciences
• /
• v.19 no.5
• /
• pp.26.1-26.8
• /
• 2016

Background: The nematode species belonging to genus Anisakis occur at their third larval stage in numerous marine teleost fish species worldwide and known to cause accidental human infection through the ingestion of raw or undercooked fish or squids. They may also draw the attention of consumers because of the visual impact of both alive and dead worms. Therefore, the information on their geographical distribution and clear species identification is important for epidemiological survey and further prevention of human infection. Results: For identification of anisakid nematodes species isolated from largehead hairtail (Trichiurus japonicus), polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of internal transcribed spacers of ribosomal DNA were conducted. Mitochondrial cytochrome c oxidase subunit 2 gene was also sequenced, and phylogenetic analysis was conducted. From the largehead hairtail (n = 9), 1259 nematodes were isolated in total. Most of the nematodes were found encapsulated throughout the viscera (56.2 %, 708/1259) or moving freely in the body cavity (41.5 %, 523/1259), and only 0.3 % (4/1259) was found in the muscles. By PCR-RFLP, three different nematode species were identified. Anisakis pegreffii was the most dominantly found (98.7 %, 1243/1259) from the largehead hairtail, occupying 98.7 % (699/708) of the nematodes in the mesenteries and 98.1 % (513/523) in the body cavity. Hybrid genotype (Anisakis simplex ${\times}$ A. pegreffii) occupied 0.5 %, and Hysterothylacium sp. occupied 0.2 % of the nematodes isolated in this study. Conclusions: The largehead hairtail may not significantly contribute accidental human infection of anisakid nematode third stage larvae because most of the nematodes were found from the viscera or body cavity, which are not consumed raw. But, a high prevalence of anisakid nematode larvae in the largehead hairtail is still in concern because they may raise food safety problems to consumers. Immediate evisceration or freezing of fish after catch will be necessary before consumption.

• Korean Journal of Environmental Biology
• /
• v.21 no.2
• /
• pp.177-180
• /
• 2003

Four genera of metazoan parasites were recovered from 3 of 6 Sebastes pinniger examined during January 2003. Two hundred forty-five metazoan parasites were recovered. Trochopus trituba Sekerak and Arai, 1977 and Anisakis larvae were found to he infected S. finniger Gill and te be abundant in this rockfish. One hundred eleven worms of monogenean trematodes and 134 larval anisakine nematodes were also found in this fishes. Precise positions with regard to gills, fins, and gastrointestinal tracts were recorded on S. pinniger to determine distribution of metazoan parasites of Trochopus trituba, Anisakis simplex larva, Contracaecum sp. larva, and Pseudo terranova decipiens larva. The monogenean trematodes, T. trituba were attacked to the gill arches and fin, and the larval anisakine nematodes, A. simplex, Contracaecum sp.,and P. decipiens were found on the visceral organs such as intestine, omentum and stomach wall. The second gill arches were the most heavily infested and posterodorsal region of the gill arches contained most monegenenn trematodes. The nam site of infestation of larval anisakine nematodes were the intestine.

• Journal of Food Hygiene and Safety
• /
• v.17 no.4
• /
• pp.206-209
• /
• 2002

From February through March 2002, sixteen rockfishes representing three species were collected from Oregon Coast Aquarium and Newport fish market and examined far nematodes. During this study, a total of 157 Anisakis type larvae were recovered from Sebastes spp. and parasitized for an average infection prevalence of 50.0%. The yellowtail rockfish, S. flavidus, darkblotched rockfish, S. crameri, and canary rockfish, S. pinniger examined were infected with 110 Anisakis simplex larva, 30 Contracaecum spp. larva and 17 Pseudoterranova decipiens larva. Of the rockfishes examined, 75.0% carried Sebastes crameri, and 12.5% S. flavidus and S. crameri, respectively. The sites of infection far Anisakis type larvae were the intestine, omentum, and stomach walls. A. simplex larvae infected 60.0% on S. crameri and 33.3% on S.flavidus and S. pinniger respectively. The prevalence of Contracaecum sp. larvae and Pseudoterranova decipiens larvae on S. pinniger was 33.3%. The intensities of A. simplex larvae ranged from 1 to 87 in S. flavidus, S. crameri, and S. pinniger. The intensity of Contracaecum spp. larvae and P. decipiens larvae ranged from 0 to 30 in S. pinniger.