• Bulletin of the Korean Chemical Society
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• 제28권10호
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• pp.1867-1870
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• 2007

• 약학회지
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• 제47권2호
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• pp.110-119
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• 2003

The purpose of the present study was to kinetically investigate the carrier-mediated uptake in the hepatic transport of organic anions, and to simulate the ″in vivo counter-transport″ phenomena, using kinetic model which was developed in this study. The condition that the mobility of carrier-ligand complex is greater than that of free carrier is not essential for the occurrence of ″counter-transport″ phenomenon. To examine the inhibitory effects on the initial uptake of a ligand by the liver, it is necessary to judge whether the true counter-transport mechanism (trans-stimulation) is working or not. The initial plasma disappearance curves of a organic anion were then kinetically analyzed based on a flow model, in which the ligand is eliminated only from the peripheral compartment (liver compartment). Moreover, ″in vive counter-transport″ phenomena were simulated based on the perfusion model which incorporated the carrier-mediated transport and the saturable intracellular binding. The ″in vivo counter-transport″ phenomena in the hepatic transport of a organic anion were well demonstrated by incorporating the carrier-mediated process. However, the ″in vivo counter-transport″ phenomena may be also explained by the enhancement of back diffusion due to the displacement of intracellular binding. In conclusion, one should be more cautious in interpreting data obtained from so-called ″in vivo counter-transport″ experiments.

• Bulletin of the Korean Chemical Society
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• 제23권1호
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• pp.119-122
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• 2002

Weakly coordinating anions show little affinity for binding to unfunctionalized iron(II) porphyrins. The electron-deficient 5, 10, 15, 20-tetrakis(pentafluorophenyl)porphinatoiron(II) compound is utilized in this study to demonstrate solution coordination by chloride, bromide and acetate ions. The binding strength of anions to the iron(II) porphyrin is reflected by a systematic change in pyrrole proton chemical shift in $^1H$ NMR spectra; the pyrrole resonance moves downfield when the ${\sigma}$-donor ability of anions is decreased.

• 한국동물학회지
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• 제37권1호
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• pp.66-75
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• 1994

Juvenile hormone binding protein was identified in the hemolymph of fifth instar larvae and purified using column chromatography. Hemolymph was mixed with [3H] JH-III and electrophoresed on 691 NON-SDS gel, indicating that radioactivity peak appears at Rf value of 0.55. Gel filtration showed two radioactivity peaks equivalent to bound and free [3H]JH-III, respectively. JHBP was purified from hemolymph through gel filtration (Sephadex G-100), anion exchange chromatosraphv (DEAE Sepharose CL-6B), chromatofocusing chromatographv (PBE 94) and preparative electrophoresis.

• EDISON SW 활용 경진대회 논문집
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• 제2회(2013년)
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• pp.77-88
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• 2013

The computational study on the fluoride ion binding with bis(bora)calix[4]arene has been performed using density functional theory and ONIOM model. The computed structure and fluorescent behavior of bis(bora)calix[4]arene was corresponded to experiment value. The binding energy for fluoride anion is computed to be 28.05kJ/mol in the chloroform solution. We also predicted that this sensing mechanism is only valid for fluoride ion in halogens. By analyzing molecular orbitals, binding with fluoride ion reduces energy differences between HOMO and LUMO, which leads to fluorescent sensing.

• Journal of Microbiology
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• 제37권1호
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• pp.21-26
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• 1999

Two novel calcium-binding proteins, named CAB-I and CAB-II, have been isolated from Streptomyces coelicolor. Purification of the calcium-binding proteins involved heat treatment, fractionation with ammonium sulfate, acid treatment, anion exchange and hydrophobic interaction column chromatography, FPLC gel filtration, and preparative isoelectric focusing. A chelex competitive assay and 45Ca autoradiography verified the calcium-binding ability of the proteins. The major band CAB-II has an apparent molecular weight of 26,000 determined by SDS-polyacrylamide gel electrophoresis and 340,000 determined by gel filtration. The isoelectric point of this molecule showed the acidic nature of the molecule. N-terminal amino acid sequence analysis shows homology to rat Ca2+/calmodulin-dependent protein kinase-II (CAB-II) and yeast phosphoprotein phosphatase (CAB-I).

• 생명과학회지
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• 제26권3호
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• pp.346-352
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• 2016

전통적으로 향기(aroma) 치료법은 대체 의학 분야에서뿐만 아니라 정신과 치료에서도 실제로 널리 활용 되었다. 본 연구에서는 일차적으로 odorant-binding protein (OBP) 활성 부위에 대한 스마트 웨이브(SW) 주요 화학적 조성물과 OBP 활성 리간드인 citrate anion 결합 친화도를 비교 분석하였다. SW는 OBP 활성 부위에 대해 citrate anion보다 훨씬 높은 분자 결합 친화도를 나타내었다. 임상적으로 SW혼합 조성물을 후각으로 흡입한 전/후, 뇌파에 어떠한 변화가 유도되는지를 비교 분석하였다. 18명의 피험자들을 대상으로 SW 흡입 전/후 뇌파의 명상(안정감) 변화 지수를, 악취를 내는 음성 대조군(EV)과 파우더향을 발산하는 양성 대조군(HB) 흡입 전/후의 변화 값과 비교하였다. SW를 흡입한 실험군에서는 SW를 흡입하고 난 후 상쾌함을 경험하였다는 반응을 나타냈으며 SW흡입군에서는 EV흡입군과 HB흡입군에 비하여 뇌파의 명상 지수가 현저하게 안정된 값으로 유도되는 것을 확인하였다. 또한 EV흡입 이후 SW를 흡입하였을 때 뇌파 명상 지수가 안정 뇌파값 범위로 개선되었다. 결과적으로 SW혼합 조성물은 OBP에 효과적으로 결합하여 OBP를 활성화 시키고, SW 흡입은 뇌파를 효과적으로 통제하여 심리적 상태를 현저하게 안정화 시키는 것을 확인하였다. 본 연구를 통하여 SW흡입법은 외부의 스트레스 자극으로부터 뇌 활성을 효율적으로 조절할 수 있는 새로운 향기 치료법으로의 활용이 가능하다고 판단된다.

• Bulletin of the Korean Chemical Society
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• 제30권9호
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• pp.2101-2106
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• 2009

A series of facial amphiphiles 3,7-diaminocholestane were synthesized from 3,7-diketocholestane via 2 sequential reductive aminations and anion recognition was evaluated with acetate, chloride, bromide, fluoride and phosphate anions. The stereo-selective reductive amination protocol was utilized to synthesized facial amphiphiles afforded receptors in high yields. The molecular receptor 2 showed the highest binding constant with acetate in a 1:1 ratio.

• 한국환경과학회지
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• 제8권2호
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• pp.205-209
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• 1999

Iron hydroxides are good adsorbents for uncomplexed metals, some metal-ligand complexes and many metal oxyanions. However, their adsorption properties of these precipitations are not fully exploited in wastewater treatment operations because of difficulties associated with their separation from the aqueous phase. This study describes experiments in which iron hydroxides were coated onto the surface of ordinary adsorbents(Sea sand) that are very resistant to acids, The coated adsorbents were used in adsorption of oxyanionic metals. The process was successful in removing some anions such as $SeO_3(-II)$ over a wide range of metal concentrations and sorption of oxyanionic metals increased with decreasing pH. Formation of two surface complexes for oxyanionic metals adsorption on iron hydroxides comprise (1) complexation of the free anion by a positively charged surface site, and (2) protonation of the adsorbed anion (or alternatively adsorption of a protonated form from solution) The coated adsorbents are inexpensive to prepare and could serve as the basis of a useful oxyanionic metal removal.

• 한국막학회:학술대회논문집
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• 한국막학회 2004년도 추계 총회 및 학술발표회
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• pp.142-145
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• 2004

Anion-exchange porous hollow-fiber membranes with a thickness of about 1.2 mm and a pore size of about $0.30{\mu}m$ were used as a supporting matrix to immobilize cycloisomaltooligosaccharide glucanotransferase (CITase). CITase was immobilized to the membrane via anion-exchange adsorption and by subsequent enzymatic cross-linking with transglutaminase, the amount of which ranged from 3 to 110 mg per g of the membrane. The degree of enzyme multilayer binding was equivalent to 0.3 to 9.8. Dextran, as the substrate, was converted into seven- to nine-glucose-membered cycloisomaltooligosaccharides (CI-7, -8, and -9) at a maxi mum yield of $28\%$ in weight at a space velocity of 10 per hour during the permeation of $2.0(w/w)\%$ dextran solution across the CITase-immobilized porous hollow-fiber membrane.