The Korean Journal of Zoology (한국동물학회지)
- Volume 37 Issue 1
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- Pages.66-75
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- 1994
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- 0440-2510(pISSN)
Hemolymph Juvenile Hormone Binding Protein of Fifth Instar Larvae of Bombyx mori L.: Identification and Purification
누에나방의 5령유충 혈림프의 유약호르몬 결합단백질: 확인 및 정제
- Park, Chul-Ho (Department of Biology, Korea University, Seoul 136-701) ;
- Kim, Hak-Ryul (Department of Biology, Korea University, Seoul 136-701)
- Published : 1994.01.01
Abstract
Juvenile hormone binding protein was identified in the hemolymph of fifth instar larvae and purified using column chromatography. Hemolymph was mixed with [3H] JH-III and electrophoresed on 691 NON-SDS gel, indicating that radioactivity peak appears at Rf value of 0.55. Gel filtration showed two radioactivity peaks equivalent to bound and free [3H]JH-III, respectively. JHBP was purified from hemolymph through gel filtration (Sephadex G-100), anion exchange chromatosraphv (DEAE Sepharose CL-6B), chromatofocusing chromatographv (PBE 94) and preparative electrophoresis.