The Korean Journal of Zoology (한국동물학회지)

The Korean Society for Integrative Biology (한국통합생물학회)
권호 표지

Hemolymph Juvenile Hormone Binding Protein of Fifth Instar Larvae of Bombyx mori L.: Identification and Purification

누에나방의 5령유충 혈림프의 유약호르몬 결합단백질: 확인 및 정제

  • Park, Chul-Ho (Department of Biology, Korea University, Seoul 136-701) ;
  • Kim, Hak-Ryul (Department of Biology, Korea University, Seoul 136-701)
  • Published : 1994.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

Juvenile hormone binding protein was identified in the hemolymph of fifth instar larvae and purified using column chromatography. Hemolymph was mixed with [3H] JH-III and electrophoresed on 691 NON-SDS gel, indicating that radioactivity peak appears at Rf value of 0.55. Gel filtration showed two radioactivity peaks equivalent to bound and free [3H]JH-III, respectively. JHBP was purified from hemolymph through gel filtration (Sephadex G-100), anion exchange chromatosraphv (DEAE Sepharose CL-6B), chromatofocusing chromatographv (PBE 94) and preparative electrophoresis.

Keywords