• Animal Bioscience
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• v.37 no.3
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• pp.481-491
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• 2024

Objective: This study determined fermentation characteristics of commonly used feedstuffs, especially tropical roughages, for dairy cattle in Southeast Asia. This information is considered relevant in the context of the observed low milk fat content and milk production in Southeast Asia countries. Methods: A total of 29 feedstuffs commonly used for dairy cattle in Vietnam and Thailand were chemically analysed and subjected to an in vitro gas production (GP) test. For 72 h, GP was continuously recorded with fully automated equipment and methane (CH₄) was measured at 0, 3, 6, 9, 12, 24, 30, 36, 48, 60, and 72 h of incubation. A triphasic, nonlinear, regression procedure was applied to analyse GP profiles while a monophasic model was used to obtain kinetics related to CH₄ production. Results: King grass and VA06 showed a high asymptotic GP related to the soluble- and non-soluble fractions (i.e. A1 and A2, respectively) and had the highest acetate to propionate ratio in the incubation fluid. The proportion of CH₄ produced (% of GP at 72 h) was found to be not different (p>0.05) between the various grasses. Among the selected preserved roughages (n = 6) and whole crops (n = 4), sorghum was found to produce the greatest amount of gas in combination with a relatively low CH₄ production. Conclusion: Grasses belonging to the genus Pennisetum, and whole crop sorghum can be considered as suitable ingredients to formulate dairy rations to enhance milk fat content in Vietnam/Thailand.

• Journal of Microbiology and Biotechnology
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• v.30 no.4
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• pp.515-525
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• 2020

Interferon (IFN)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. Lactobacillus plantarum (L. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. Alphacoronaviruses, especially porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV), cause high morbidity and mortality in piglets resulting in economic loss. Co-infection by these two viruses is becoming increasingly frequent. Therefore, it is particularly important to develop a new drug to prevent diarrhea infected with mixed viruses in piglets. In this study, we first constructed an anchored expression vector with CWA (C-terminal cell wall anchor) on L. plantarum. Second, we constructed two recombinant L. plantarum strains that anchored IFN-λ3 via pgsA (N-terminal transmembrane anchor) and CWA. Third, we demonstrated that both recombinant strains possess strong antiviral effects against coronavirus infection in the intestinal porcine epithelial cell line J2 (IPEC-J2). However, recombinant L. plantarum with the CWA anchor exhibited a more powerful antiviral effect than recombinant L. plantarum with pgsA. Consistent with this finding, Lb.plantarum-pSIP-409-IFN-λ3-CWA enhanced the expression levels of IFN-stimulated genes (ISGs) (ISG15, OASL, and Mx1) in IPEC-J2 cells more than did recombinant Lb.plantarum-pSIP-409-pgsA'-IFN-λ3. Our study verifies that recombinant L. plantarum inhibits PEDV and TGEV infection in IPEC-J2 cells, which may offer great potential for use as a novel oral antiviral agent in therapeutic applications for combating porcine epidemic diarrhea and transmissible gastroenteritis. This study is the first to show that recombinant L. plantarum suppresses PEDV and TGEV infection of IPEC-J2 cells.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.11
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• pp.1560-1564
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• 2003

The study was carried out in Sahiwal, Holstein Friesian, Jersey and crossbred cattle to find out the effect of genotype of beta-lactoglobulin gene on milk production traits. The polymorphism at beta-lactoglobulin gene was identified by conducting PCRRFLP studies. A 398 bp fragment of the gene was amplified and digested with Hae III restriction enzyme. The two alleles A and B and three genotypes AA, AB and BB were identified in all cattle breeds. The frequency of B allele was comparatively higher than that of A allele. The AA genotype produced significantly higher milk yield in Sahiwal cattle whereas BB genotype yielded higher milk in Holstein friesian cattle. In other cattle breeds the genotypic effect was non-significant. In conclusion it may be stated that the genotype with significantly higher milk yield may be favoured in the farm along with other conventional selection criteria to enhance the milk production of animals.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.1
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• pp.2-11
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• 2020

Assisted reproductive technologies (ART) merely depend on improving the oocyte maturation and their developmental competence to produce good quality embryos. Oocyte maturation passes through long and complex molecular steps starts from the early embryonic life and ends with sperm fertilization. Oocyte developmental competence can be attained by improving the nuclear and cytoplasmic mechanisms together with some epigenetic maturation. In this review, we highlight the cornerstones of oocyte maturation on both nuclear and cytoplasmic levels. Interfering or supporting these molecular mechanisms would help in the development of novel regulating agents for reproductive performance of humans and livestock species.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.10
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• pp.1565-1574
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• 2018

Objective: The aim of the present study was to identify genetic variants based on RNA-seq data, obtained via transcriptome sequencing of muscle tissue of pigs differing in muscle histological structure, and to verify the variants' effect on histological microstructure and production traits in a larger pig population. Methods: RNA-seq data was used to identify the panel of single nucleotide polymorphisms (SNPs) significantly related with percentage and diameter of each fiber type (I, IIA, IIB). Detected polymorphisms were mapped to quantitative trait loci (QTLs) regions. Next, the association study was performed on 944 animals representing five breeds (Landrace, Large White, Pietrain, Duroc, and native Puławska breed) in order to evaluate the relationship of selected SNPs and histological characteristics, meat quality and carcasses traits. Results: Mapping of detected genetic variants to QTL regions showed that chromosome 14 was the most overrepresented with the identification of four QTLs related to percentage of fiber types I and IIA. The association study performed on a 293 longissimus muscle samples confirmed a significant positive effect of transforming acidic coiled-coil-containing protein 2 (TACC2) polymorphisms on fiber diameter, while SNP within forkhead box O1 (FOXO1) locus was associated with decrease of diameter of fiber types IIA and IIB. Moreover, subsequent general linear model analysis showed significant relationship of FOXO1, delta 4-desaturase, sphingolipid 1 (DEGS1), and troponin T2 (TNNT2) genes with loin 'eye' area, FOXO1 with loin weight, as well as FOXO1 and TACC2 with lean meat percentage. Furthermore, the intramuscular fat content was positively associated (p<0.01) with occurrence of polymorphisms within DEGS1, TNNT2 genes and negatively with occurrence of TACC2 polymorphism. Conclusion: This study's results indicate that the SNP calling analysis based on RNA-seq data can be used to search candidate genes and establish the genetic basis of phenotypic traits. The presented results can be used for future studies evaluating the use of selected SNPs as genetic markers related to muscle histological profile and production traits in pig breeding.

• Asian-Australasian Journal of Animal Sciences
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• v.2 no.3
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• pp.243-245
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• 1989

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.2
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• pp.215-219
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• 1999

This study aims to make detail examination of smallholder dairy farming systems in the Nongpho Dairy Cooperative. Forty-three dairy farms were selected from three geographical areas i.e. irrigated area, municipality area, and factory area. Within each area some number of sample farms were selected from each of the three levels of farm and animal crowdedness (very crowded, crowded, and not crowded farms). Detail data were collected during 1996 to 1997, they were socio-economic conditions of the sample farms and farmers, dairy production systems and management of resources (animals, bam, feeds, stocking rates, herd structure, animal body conditions, milk yield and milk quality, manure and farm wastes management, and other related items). Detail information useful for the improvement of farm production efficiency were discussed. It was very clear that much improvement of smallholder dairy production can be achieved if the recommendations given by this study were implemented.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.9
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• pp.1263-1266
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• 2002

The objective of this study was to evaluate the effects of some production factors on commercial production of Etawah Crossbred Goats (ECG) of Inpres Desa Tertinggal member groups (AKIDT) at Krasak, Pandansari, Brajan, and Kragilan villages in Boyolali regency, Central Java, Indonesia. The study was from February to April 2000. Eighty respondents of AKIDT were selected by simple random sampling and the data were analyzed using Cobb Douglas Production Function. The results showed that ECG production simultaneously were highly significant (p<0.01) influenced by amount of feed consumed (kg TDN/year, $x_1$), number of does of ECG (Animal Unit/year, $x_2$), number of kids and does/ barn/year (Animal Unit/year, $x_3$), labor use (man-days/year, $x_4$) and work capital (US$/year, $x_5$) with $R^2= 0.6568$. In addition, ECG production was partially influenced by $x_2$, $x_3$ and $x_5$ (p<0.01) and $x_1$ (p<0.05), but not significant (p>0.05) by $x_4$. Technically, production factors of $x_1$, $x_2$, $x_3$, $x_4$, $x_5$ had reached technique efficiency (0$x_1$, $x_3$, $x_4$, $x_5$ did not showed efficiency (Ep<1) and $x_2$ was not efficient yet.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.2
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• pp.157-163
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• 1997

This paper analyses a number of important areas relating to methane production in ruminants, consequent hazards and different methods of reducing this gas. Clearly methane not only affects on the environment but also on the economy of animal production. Several factors including feed, species, microbes, rumen environment, etc. are responsible for methane production in animals. Although methane production can be reduced by chemical manipulation, defaunation and strategic feeding, the latter was found to be effective because the method is easier to follow than the others. Furthermore, feeding technology could play an important role in reducing methane production particularly in developing countries because of its relative cost effectiveness. however, it needs to compare to what extent it could reduce methane production as well as cost of animal production. Therefore, research program needs to be concentrated on the appropriate feeding system to reduce methane production, consequently pollution and cost of production particularly in developing countries.

• Asian-Australasian Journal of Animal Sciences
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• v.4 no.2
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• pp.165-168
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• 1991

The maternal weigh-suckle-weigh (WSW) method for estimating milk production of sows was further evaluated by comparing this to the traditional WSW method. Twenty one estimates of hourly milk production were obtained by both methods. Total hourly milk production between the two methods was not significantly different (292.4 vs 303.3 g/h, p > 0.05). Hourly milk production determined by the maternal WSW method was highly correlated with hourly milk production estimates using the traditional WSW method ($R_2$ = 0.94, p < 0.001). When corrections for metabolic and salivary losses were made, the milk production figures for the maternal WSW method were approximately 27% less than those estimated by the traditional WSW method.