• 생명과학회지
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• 제21권10호
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• pp.1428-1433
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• 2011

본 연구에서는 ACE 저해능 및 항산화능이 있는 식물성 젖산균을 다양한 식물체로부터 분리한 후, 그 특성을 조사하였다. 김치, 부추, 포도 및 동동주에서 K-1, K-21, L-5, G-3 및 D-3 균주가 분리되었으며, 16S rRNA gene 염기서열 분석을 통하여 이들은 각각 Pediococcus pentosaceus, Lactobacillus plantarum, Weissella cibaria, L. plantarum 및 L. brevis로 동정되었다. 분리균주들은 MRS broth에서 44.3-71.9%의 ACE 저해능을 나타내었으며, 특히 G-3, L-5, K-1 균주는 skim milk가 함유된 MRS broth에서 59.0-8-98.6%의 높은 ACE 저해능을 나타내었다. 분리 균주는 42.5-82.7%의 DPPH radical 소거능을 나타내었으며, G-3 및 K-1 균주는 pH 2.5의 인공위액에서 42.2-88.1%의 높은 생존율을 나타내었다. 분리균주는 0.3% oxgall에서 24시간 배양시 55.4-112.8%의 내성을 나타내었다. 또한 분리균주는 유기산 생성에 따른 pH 감소 효과로 인하여 Pseudomonas aeruginosa를 포함한 일부 병원성 세균의 생육을 억제할 수 있었다.

• 사상체질의학회지
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• 제11권2호
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• pp.185-194
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• 1999

근래에 유전자를 이용하여 체질(體質)을 객관화하려는 시도가 있었다. 이에 본인은 그 동안 한의학계(韓醫學係)에서 이루어진 유전자를 이용한 사상체질(四象體質)의 객관화 연구와 의학계 쪽의 연구 중에서 어느 정도 연관성이 있는 논문들을 비교 검토하여 앞으로 사상의학(四象醫學)에서 유전자를 이용한 연구를 하는데 있어서 방향설정을 하는데 도움이 되고자 본 논문을 쓰게 되었다. 그 결과를 요약하면 다음과 같다. 1. 유전자 polymorphism(다형성)이 기능성을 가진 것을 대상으로 하여야한다. 2. Microsatellite는 기능을 가지지 않는 부분이므로 이것의 polymorphism이 체질(體質)과 관련될 가능성은 적을 것으로 사료된다. 3. Angiotensin converting enzyme (ACE)은 일반적으로 체질(體質)과 관련이 있다고 믿어지는 인내력(忍耐力)을 결정하는데 중요한 유전자로 알려져 있는 바 이 효소의 polymorphism을 이용하여 체질(體質)을 분류하고자 한 시도는 의미가 있는 것으로 생각된다. 4. HLA는 다형성을 가지고 있고 HLA유전자의 발현에 따라 질병에 대한 감수성이 차이가 있다는 사실을 고려해 볼 때 앞으로 이 분야는 연구해볼 충분한 가치가 있는 것으로 생각된다. 5. DNA chip의 사용이 보편화되면 사상체질(四象體質)의 객관화에 많은 도움이 될 것으로 생각된다.

• The Korean Journal of Physiology and Pharmacology
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• 제19권2호
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• pp.131-139
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• 2015

This study analyzed the differences in aerobic and anaerobic exercise ability and growth-related indicators, depending on the polymorphism of the ACE and the ACTN3 genes, to understand the genetic influence of exercise ability in the growth process of children. The subjects of the study consisted of elementary school students (n=856, age $10.32{\pm}0.07yr$). The anthropometric parameters, physical fitness and growth factors were compared among groups of the ACE I/D or the ACTN3 R577X polymorphisms. There were no significant differences between the anthropometric parameters, physical fitness and growth factors for the ACE gene ID or the ACTN3 gene R577X polymorphism. However, the DD type of ACE gene was highest in the side step test (p<0.05), and the DD type was significantly higher than the II+ID type (p<0.05) in the early bone age. The combined group of the ACE gene II+ID and the ACTN3 gene XX type significantly showed lower early bone age (p< 0.05). This study did not find any individual or compounding effects of the polymorphism in the ACE I/D or the ACTN3 R577X polymorphisms on the anthropometric parameters, physical fitness and growth factors of Korean children. However, the exercise experience and the DD type of the ACE gene may affect the early maturity of the bones.

• Mycobiology
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• 제48권5호
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• pp.399-409
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• 2020

Many of the 𝛽-glucans are known to have antihypertensive activities, but, except for angiotensin-converting enzyme II inhibition, the underlying mechanisms remain unclear. Corin is an atrial natriuretic peptide (ANP)-converting enzyme. Activated corin cleaves pro-ANP to ANP, which regulates water-sodium balance and lowers blood pressure. Here, we reported a novel antihypertensive mechanism of 𝛽-glucans, involved with corin and ANP in mice. We showed that multiple oral administrations of 𝛽-glucan induced the expression of corin and ANP, and also increased natriuresis in mice. Microarray analysis showed that corin gene expression was only upregulated in mice liver by multiple, not single, oral administrations of the 𝛽-glucan fraction of Phellinus baumii (BGF). Corin was induced in liver and kidney tissues by the 𝛽-glucans from zymosan and barley, as well as by BGF. In addition to P. baumii, 𝛽-glucans from two other mushrooms, Phellinus linteus and Ganoderma lucidum, also induced corin mRNA expression in mouse liver. ELISA immunoassays showed that ANP production was increased in liver tissue by all the 𝛽-glucans tested, but not in the heart and kidney. Urinary sodium excretion was significantly increased by treatment with 𝛽-glucans in the order of BGF, zymosan, and barley, both in 1% normal and 10% high-sodium diets. In conclusion, we found that the oral administration of 𝛽-glucans could induce corin expression, ANP production, and sodium excretion in mice. Our findings will be helpful for investigations of 𝛽-glucans in corin and ANP-related fields, including blood pressure, salt-water balance, and circulation.

• 생명과학회지
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• 제19권9호
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• pp.1277-1283
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• 2009

본 연구는 대한민국 공군사관생도들을 대상으로 12주간 복합 트레이닝(유/무산소성 트레이닝, 공간지각 트레이닝)이 Angiotensin Converting Enzyme (ACE) gene polymorphism에 따른 신체조성과 심폐지구력 변화에 미치는 영향을 규명하는데 목적이 있었다. 연구결과, 12주간 복합 트레이닝 후 신체조성은 ACE gene polymorphism에 따라 변화의 차이가 나타나지 않았으며, 심폐지구력은 I allele 집단(II/ID genotype group)이 D allele 집단(DD genotype group)보다 트레이닝의 효과가 높게 나타났다. 본 연구 결과로 볼 때, 복합 트레이닝은 ACE gene polymorphism에 따라 심폐지구력 변화에 다르게 반응하고 있으며, I allele (II/ID genotype group)를 가지고 있는 개체가 더 높은 트레이닝의 효과를 얻을 수 있음을 제시해 주고 있다.

• Journal of Ginseng Research
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• 제36권2호
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• pp.176-189
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• 2012

This study examined the effects of Korean red ginseng (KRG) on obese women and aimed to confirm that the effects of KRG on obesity differ dependently on a gene. Fifty obese women were recruited and randomized to receive KRG (n=24) or placebo (n=26) for 8 wk. Measurements of blood pressure, height, weight, waist circumference, waist-hip ratio (WHR), total fat mass, percentage of body fat, resting metabolic rate, basal body temperature, and daily food intake (FI), blood test (serum lipid, liver and renal function), Korean version of obesity-related quality of life scale (KOQOL), and a gene examination were performed. Comparisons of subjects before and after the administration of KRG revealed significant improvements of obesity in terms of weight, body mass index (BMI), WHR, FI, and KOQOL. However, in the comparison between KRG group and placebo group, only KOQOL was significantly different. KRG displayed significant efficacy on BMI and KOQOL in the CT genotype of the G protein beta 3 gene, but not in the CC genotype, on blood sugar test in the Trp64/Arg genotype of the beta 3 adrenergic receptor gene, but not in Trp64/Trp genotype, on KOQOL in the DD genotype of the angiotensin I converting enzyme gene, but not in the ID and DD genotypes. The effects of KRG on obesity were confirmed to some extent. However, a distinct effect compared to placebo was not confirmed. KRG is more effective for improving the secondary issues of the quality of life derived from obesity rather than having direct effects on the obesity-related anthropometric assessment and blood test indices.

• 사상체질의학회지
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• 제10권2호
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• pp.283-290
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• 1998

Sasang Constitutional Medicine focuses on the different constitutional manifestations of the individual's nature and emotions. The nature and emotions drive the ascending and descending of Qi in the body. And this dynamics of the Qi's ascent and descent shapes the different types of structures, functions and temperaments. Although Sasang Constitutional Medicine has many advantages, its diagnosis of the constitution still depends on the doctors' own idea and has no objective identification. So many doctors in Korea have been trying to solve this problem. Recently, there are several efforts to find out the relationship between genetic information and constitution. By the way, May, 1998 there is a astonishing report about the gene which determines the human performance, that is ACE(angiotensin converting enzyme). And it suggests that the I allele was associated with improved endurance performance. ACE has three genotype including II, ID and DD. "I" means insertion and "D" means deletion. We determined the type of the Sasang constitution with QSCCII questionaire and the one's ACE genotype with PCR of the 127 people and we discovered the relationship between the constitution and the ACE genotype. The result is as follow. Among 39 people who have the II genotype, 7(18%) belong to Taeum(Taiyin), 9(23.1%) belong to Soyang(Shaoyang) and 23(59%) belong to Soeum(Shaoyin). Among 62 people who have the ID genotype, 18(29%) belong to Taeum(Taiyin), 21(33.9%) belong to Soyang(Shaoyang) and 23(37.1%) belong to Soeum(Shaoyin). Among 26 people who have DD genotype, 11(42.3%) belong to Taeum(Taiyin), 4(15.4%) belong to Soyang(Shaoyang) and 11(42.3%) belong to Soeum(Shaoyin). This data indicates that there are implicable relationship between the Sasang constitution and ACE genotype. Especially people who have II genotype have much possibility to be a Soeum(Shaoyin) person (59%) and Soyang(Shaoyang) person have less possibility to have DD genotype (15.4%). With this conclusion, we suggest further study of relationship between the Sasang constitution and ACE genotype and we think that other polymorphism can be a candidate of the partner of Sasang constitution.

• Environmental Analysis Health and Toxicology
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• 제17권4호
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• pp.299-305
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• 2002

심폐 지구력은 유전인자에 의해 부분적으로 결정되며, 현재까지 이루어진 연구 결과에 의하면 안지오텐신 전환효소 유전자에 존재하는 다형성과 이 형질 사이에 유의한 관련성이 보고되고 있다. 그러나, 이러한 연구는 주로 서양인을 대상으로 수행되었기 때문에 유전적 배경이 다른 아시아 집단에 대해서는 아직까지 이렇다 할 연구 성과가 없는 실정이다. 이에, 본 연구에서는 아시아 집단중에서도 민족적으로 순수한 한국인 집단을 대상으로 안지오텐신 전환효소 유전자에 존재하는 다향성이 한국인 집단에서도 심폐 지구력과 유의한 관련성이 있는 지를 조사하였다. 그러나, 한국인 운동선수군을 대상으로 한 연구에서는 안지오텐신 전환효소 유전자의 다향성이 심폐 지구력을 비롯한 신체 계측치 및 생화학적인 측정치 들과 어떠한 관련성도 나타내지 않았다(P＜0.05). 그러나, 본 연구 대상으로 다양한 종목에서 선발된 운동선수들을 표본으로 하였기 때문에, 단일 종목의 운동 선수군을 대상으로 한 추시가 요구된다.

• Clinical and Experimental Pediatrics
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• 제55권2호
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• pp.48-53
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• 2012

Purpose: Gaucher disease is caused by a ${\beta}$-glucocerebrosidase (GBA) deficiency. The aim of this study is to investigate the clinical and genetic characteristics according to subtypes of Gaucher disease in the Korean population. Methods: Clinical findings at diagnosis, $GBA$ mutations, and clinical courses were reviewed in 20 patients diagnosed with Gaucher disease. Results: Eleven patients were diagnosed with non-neuronopathic type, 2 with acute neuronopathic type, and 7 with chronic neuronopathic type. Most patients presented with hepatosplenomegaly, thrombocytopenia, and short stature. In the neuronopathic group, variable neurological features, such as seizure, tremor, gaze palsy, and hypotonia, were noted at age $8.7{\pm}4.3$ years. B cell lymphoma, protein-losing enteropathy, and hydrops fetalis were the atypical manifestations. Biomarkers, including chitotriosidase, acid phosphatase, and angiotensin-converting enzyme, increased at the initial evaluation and subsequently decreased with enzyme replacement treatment (ERT). The clinical findings, including hepatosplenomegaly, thrombocytopenia, and skeletal findings, improved following ERT, except for the neurological manifestations. L444P was the most common mutation in our cohort. One novel mutation, R277C, was found. Conclusion: Although the clinical outcome for Gaucher disease improved remarkably following ERT, the outcome differed according to subtype. Considering the high proportion of the neuronopathic form in the Korean population, new therapeutic strategies targeting the central nervous system are needed, with the development of a new scoring system and biomarkers representing clinical courses in a more comprehensive manner.

• Journal of Microbiology and Biotechnology
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• 제17권6호
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• pp.952-959
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• 2007

His-His-Leu (HHL), a tripeptide derived from a Korean soybean paste, is an angiotensin-I-converting enzyme (ACE) inhibitor. We report here a method of producing this tripeptide efficiently by expressing tandem multimers of the codons encoding the peptide in E. coli and purifying the HHL after hydrolysis of the peptide multimers. The HHL gene, tandemly multimerized to a 40-mer, was ligated with ubiquitin as a fusion gene (UH40). UH40 was inserted into vector pET29b; the UH40 fusion protein was then produced in E. coli BL21. The recombinant UH40 protein was purified by cation-exchange chromatography with a yield of 17.3mg/l and analyzed by matrixassisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry and protein N-terminal sequencing. Leucine aminopeptidase was used to cleave a 405-Da HHL monomer from the UH40 fusion protein and the peptide was purified using reverse-phase high-performance liquid chromatography (HPLC) on a C18 HPLC column, with a final yield of 6.2mg/l. The resulting peptide was confirmed to be HHL with the aid of MALDI-TOF mass spectrometry, glutamine-TOF mass spectrometry, N-terminal sequencing, and measurement of ACE inhibiting activity. These results suggest that our production method is useful for obtaining a large quantity of recombinant HHL for functional antihypertensive peptide studies.