• Journal of Korean Medicine Rehabilitation
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• v.28 no.2
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• pp.47-60
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• 2018

Objectives The objective of this study was to investigate the effect of Anti-condensing on the composition of ChondroT Methods Specimens are divided in 7 groups (Control, ChondroT, Lonicerae Folium (Gumenhwa, GEH), Angelicae Gigantis Radix(Danggui, DG), Phellodendri Cortex(HwangBaek, HB), Osterici Radix(Kanghwal, KH), Clematidis Radix(Weeryungsun, WRS)) Each specimen is subjected to a concentration of 20 %, 10 %, and 5 %, and is administered to collagen and thrombin-stimulated platelets. Results In the anticoagulance effect test, Lonicerae Folium and ChondroT very well. The effect was high in order of Lonicerae Folium-Angelicae Gigantis-Phellodendri Cortex-Osterici Radix and Clematidis Radix. Conclusions ChondroT has anti-condensing effects on blood platelet.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.17 no.1
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• pp.131-142
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• 2004

The purpose of this research was to investigate cytotoxity of Sunbanhwalmyungeum extract. Cytotoxity was determined by MTT assay method. After tumor cell lines(G361, BI6F10, MDA, A549) transplantation, the extracts of SunBangHwalMyungEum and its composition oriental medicines were administered, cytotoxity was measured by absorbance. The results were obtained as follows. 1. Sunbanhwalmyungeum extract and its composition oriental medicines extracts showed the concentration was higher, the more cytotoxity increased. 2. Both water and ethanol extracts of Sunbanhwalmyungeum showed excellent cytotoxity against G361, B16F10, MDA, A549 and high cytotoxity over 80$\%$ against G361, B16F10, MDA except A549 at the concentration of 1000ppm. 3. In water extract, Rhei Radix et Rhizoma, Gleditsiae Spina, Trichosanthis Radix, Glycyrrhizae Radix, Ledebouriellae Radix showed excellent cytotoxity. In ethanol extract, Gleditsiae Spina, Citri Pericarpium, Trichosanthis Radix, Paeoniae Radix Rubra, Myrrha showed excellent cytotoxity. 4. Rhei Radix et Rhizoma, Gleditsiae Spina, Trichosanthis Radix, Paeoniae Radix Rubra showed high cytotoxity in both water and ethanol extrats. 5. In water extract, Glycyrrhizae Radix, Ledebouriellae Radix, Myrrha showed high cytotoxity against A361, Lonicerae Flos, Olibanum, Fritillariae cirrhosae Bulbus, Paeoniae Radix Rubra, Manitis Squama against B16F10, Paeoniae Radix Rubra, Manitis Squama against MDA, Rhei Radix et Rhizoma, Angelicae gigantis Radix against A549. 6. In ethanol extract, Lonicerae Flos, Trichosanthis Radix showed high cytotoxity against G361, Rhei Radix et Rhizoma, Angelicae gigantis Radix, Gleditsiae Spina, Olibanum, Angelicae dahuricae Radix, Fritillariae cirrhosae Bulbus, Paeoniae Radix Rubra, Glycyrrhizae Radix, Ledebouriellae Radix, Myrrha against B16F10, Rhei Radix et Rhizoma, Manitis Squama against MDA, Citri Pericarpium, Manitis Squama against A549.

• Korean Journal of Pharmacognosy
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• v.29 no.4
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• pp.402-412
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• 1998

This study was attempted to investigate the effect of 'Angelicae gigantis Radix extract (AG.EX.)' and 'Angelicae acutilobae Radix extract (AA.EX.)' on the activities of GOT, GPT and alkaline phosphatase (A1.P), the contents of total cholesterol in serum of $CCl_4$ and D-galactosamine intoxicated rats, and the weight change ratio of body, liver and spleen in $CCl_4-intoxicated-rats$ by administering the extract of 300 and 500 mg/kg P.O.. Significant test was performed by comparision with the biochemical values between intoxicated-control group and extract-administered group. The activities of s-GOT, s-GPT and the contents of total cholesterol elevated by $CCl_4-intoxication$ were significantly decreased in all dose (300, 500 mg/kg) of Angelicae gigantis Radix-water extract (AG.WEX.) and alcohol extract (AG.AEX), and Angelica acutilobae Radix-water extract (AA.WEX.) and alcohol extract (AA.AEX.), respectively, as compared with the control group. And administered group of 300 mg/kg showed more significant decreasing effect than 500 mg/kg, and more significantly decreased in water extract of AG.EX. and ethanol extract of AA.EX. But in the activities of s-A1.P. inhibition effect were significantly decreased only in a dose of 300 mg/kg of AA.WEX. and AA.AEX. The activities of s-GOT and s-GPT elevated by D-galactosamine were not decreased in all samples, as compared to intoxicated-control group. But the activities of s-Al.p was significantly decreased as compared with control groups, in all samples and administration of 300 mg/kg was more significantly decreased than 500 mg/kg. The contents of total cholesterol remarkably decreased than the normal groups by D-galactosamine intoxication was not recovered in all samples. The increasing rate of the body weight increased by $CCl_4-intoxication$ were not decreased than the $CCl_4-control$ group in all sample groups. The increasing rate of liver weight increased by $CCl_4-intoxication$ were significantly decreased in 300 mg/kg of AA.AEX.AG.WEX. and AA.WEX., respectively, as compared with $CCl_4-control$ group.

• Food Science and Biotechnology
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• v.14 no.4
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• pp.537-539
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• 2005

Potential of mass spectrometer-based electronic nose to discriminate habitat of Angelicae gigantis radix was evaluated using 24 and 86 Korean and non-Korean samples, respectively. Loading plot(s) of principal component analysis of data measured through this system revealed difference between Korean samples (probability; 100%) and non-Korean ones (probability; 95.3%), suggesting this technique could be used as efficient method to differentiate habitat of A. gigantis radix.

• Natural Product Sciences
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• v.10 no.6
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• pp.262-267
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• 2004

A reversed-phase liquid chromatographic method for decursin and decursinol angelate of Angelicae gigantis Radix, an important crude drug in Korean traditional medicine, was developed and validated. Decursin and decursinol angelate, the structure isomer (pyranocoumarin) each other, are the main organic constituents in Angelicae gigantis Radix. This method was developed using a RP-18 column, UV detector at 280 nm and 50% acetonitrile solution containing 0.01 M sodium dodecyl sulfate and 25 mM sodium dihydrogen phosphate (pH 5.0) as the mobile phase. Various validation parameters were included and evaluated satisfactorily. Linearity was established in range 2-75 mg/ml of decursin and decursinol angelate (correlation coefficient = 0.9997 and 0.9995, respectively). This analytical method showed good accuracy (98.1% and 99.5%, respectively). Precision (repeatability) revealed a relative standard deviation value of 1.71% (decursin) and 3.19% (decursinol angelate). For intermediate precision measure the considered variables were equipment and days. A robustness test showing the influence of deferent counter-ion concentration in mobile phase was also performed.

• Natural Product Sciences
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• v.7 no.2
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• pp.53-59
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• 2001

The possibility of Chungpesagan-tang, which has been recommended on the stroke patients with constipation in Korean traditional clinic, and its ingredients as a novel antithrombotic agent was evaluated. Most of its ingredients except Puerariae Radix exhibited in vitro antiplatelet aggregation activity. However, Puerariae Radix was significantly effective on ex vivo anti-platelet aggregation activity, whereas Angelicae Tenuissimae Radix, Raphani Semen and Angelicae Dahuricae Radix was not effective. Plasma recalcification was potently inhibited only by Puerariae Radix and Rhei Rhizoma treated with intestinal bacteria. Urokinase was also activated only by Chungpesagan-tang, Angelicae Tenuissimae Radix and Puerariae Radix treated with intestinal bacteria. Chungpesagan-tang exhibited the potent anti-thromboembolic activity activity in vitro. These results suggest that anti-thrombotic activity of Chungpesagan-tang should be activated by intestinal bacteria and may be important in the prevention of thrombosis and cardiovascular diseases, such as myocardial infraction stroke and arteriosclerosis.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.1
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• pp.60-65
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• 1993

This experiment was conducted to understand the effects of climatic environment on the useful components in Angelicae gigantis radix. The variation of decursin contents in different seven cultivated regions and the relationship between decursin contents and meteorological factors were investigated. The appropriate analytical methods for decursin and decursinol angelate of Angelicae gigantis radix were HPLC method used normal phase column($\mu$-Porasil) and GC method. In different cultivated regions, the variation of decursin contents of Angelicae gigantis radix was relatively great, which were high with 4.86%, 4.75% in Bongwha, Yongcheon respectively and was low with 2.33% in Suwon. There was no significant relationship between decursin contents of Angelicae radix and meteorological factors during growing periods, but was negative correlation in average temperature and precipitation, and positive correlation in diurnal change of temperature, radiation and sunshine hours.

• The Journal of Internal Korean Medicine
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• v.21 no.1
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• pp.23-30
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• 2000

Objectives : To analyze the effects of Samul-Tang and combinations of constituent herbs on arterial contraction. Method : In order to investigate the effects, Angelicae gigantis Radix, Cnidii Rhizoma, Rehmanniae Radix, Paeoniae Radix, in which one of them, three of them, and all of them, were used to exam. Results : Samul-Tang significantly inhibited the contraction of artery induced by PE(phenylephrine), accordingly as the concentration of Samul-Tang increased and inhibited in both with intact and removed endothelium. Among the constituent herbs of Samul-Tang, Paeoniae Radix and Paeoniae Radix-Cnidii Rhizoma combination inhibited the PE-induced contraction of artery the most. Conclusions : Samul-Tang and constituent herbs of Samul-Tang inhibit the contraction of artery.

• Korean Journal of Plant Resources
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• v.14 no.1
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• pp.24-31
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• 2001

The medicinal constituents contained in Cinidil Rhizoma and Angelicae Radix cultivated in Korea and China, were compared by confirming their qualities. From Cinidil Rhizoma and Angelicae Radix cultivated in Korea and China, butyl phthalide, senkyunolide, and ligustilide etc. phthalides were identified by GC/CMS analysis. Through GC/FID analysis, the senkyunolide content of Cinidil Rhizoma cultivated in Korea was similar to that cultivated in China. The ligustilide content of Ciniail Rhizoma cultivated in Korea was more than that cultivated in China, except for one cultivated at Ankuk province. In the case of Angelicae Radix, the ligustilide content of that cultivated in Korea was more than one cultivated at Yeungil province in China. As for quality, Cinidil Rhizoma and Angelicae Radix cultivated in Korea were better than those cultivated in China since Chinese products possessed more butilidene phthalide photo-decomposed from ligustilide, caused by long storage during the circulation processes, than that of the domestic products.

• The Korea Journal of Herbology
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• v.28 no.5
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• pp.39-44
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• 2013

Objectives : Allergy is an immune dysfunction caused by degranulation from mast cells in the early phase of allergic disease. The purpose of this study was to investigate the anti-allergic effect of fermented Angelicae gigantis Radix in human mast cell line, HMC-1. Method : The Angelicae gigantis Radix was fermented by Lactobacillus acidophilus. The cell toxicity of fermented Angelicae gigantis Radix(FAGR) was determined by MTT assay. The release of ${\beta}$-hexosaminidase from HMC-1 stimulated by phorbol-12-myristate 13-acetate (PMA) plus A23187 was determined by ${\beta}$-hexosaminidase assay. Also, the concentrations of cytokines (interleukin-$1{\beta}$, -6, -8 and tumor necrosis factor-alpha) were measured by enzyme-linked immunosorbent assay. The gene expression of COX-2 from HMC-1 stimulated by phorbol-12-myristate 13-acetate (PMA) plus A23187 was determined by reverse transcription polymerase chain reaction. The release of histamine on substance P-stimulated HMC-1 was measured by histamine assay. Result : The FAGR suppressed the release of ${\beta}$-hexosaminidase, a marker of degranulation, from HMC-1 stimulated by PMA plus A23187. The FAGR inhibited the production of interleukin-$1{\beta}$, -6, -8 and tumor necrosis factor-alpha. The FAGR inhibited the expression of COX-2 mRNA. The FAGR suppressed the release of histamine on substance P-stimulated HMC-1. Conclusion : These results provide that FAGR may be beneficial in the treatment of allergic inflammatory disease.