• 한방재활의학과학회지
• /
• 제28권2호
• /
• pp.47-60
• /
• 2018

Objectives The objective of this study was to investigate the effect of Anti-condensing on the composition of ChondroT Methods Specimens are divided in 7 groups (Control, ChondroT, Lonicerae Folium (Gumenhwa, GEH), Angelicae Gigantis Radix(Danggui, DG), Phellodendri Cortex(HwangBaek, HB), Osterici Radix(Kanghwal, KH), Clematidis Radix(Weeryungsun, WRS)) Each specimen is subjected to a concentration of 20 %, 10 %, and 5 %, and is administered to collagen and thrombin-stimulated platelets. Results In the anticoagulance effect test, Lonicerae Folium and ChondroT very well. The effect was high in order of Lonicerae Folium-Angelicae Gigantis-Phellodendri Cortex-Osterici Radix and Clematidis Radix. Conclusions ChondroT has anti-condensing effects on blood platelet.

• 한방안이비인후피부과학회지
• /
• 제17권1호
• /
• pp.131-142
• /
• 2004

The purpose of this research was to investigate cytotoxity of Sunbanhwalmyungeum extract. Cytotoxity was determined by MTT assay method. After tumor cell lines(G361, BI6F10, MDA, A549) transplantation, the extracts of SunBangHwalMyungEum and its composition oriental medicines were administered, cytotoxity was measured by absorbance. The results were obtained as follows. 1. Sunbanhwalmyungeum extract and its composition oriental medicines extracts showed the concentration was higher, the more cytotoxity increased. 2. Both water and ethanol extracts of Sunbanhwalmyungeum showed excellent cytotoxity against G361, B16F10, MDA, A549 and high cytotoxity over 80$\%$ against G361, B16F10, MDA except A549 at the concentration of 1000ppm. 3. In water extract, Rhei Radix et Rhizoma, Gleditsiae Spina, Trichosanthis Radix, Glycyrrhizae Radix, Ledebouriellae Radix showed excellent cytotoxity. In ethanol extract, Gleditsiae Spina, Citri Pericarpium, Trichosanthis Radix, Paeoniae Radix Rubra, Myrrha showed excellent cytotoxity. 4. Rhei Radix et Rhizoma, Gleditsiae Spina, Trichosanthis Radix, Paeoniae Radix Rubra showed high cytotoxity in both water and ethanol extrats. 5. In water extract, Glycyrrhizae Radix, Ledebouriellae Radix, Myrrha showed high cytotoxity against A361, Lonicerae Flos, Olibanum, Fritillariae cirrhosae Bulbus, Paeoniae Radix Rubra, Manitis Squama against B16F10, Paeoniae Radix Rubra, Manitis Squama against MDA, Rhei Radix et Rhizoma, Angelicae gigantis Radix against A549. 6. In ethanol extract, Lonicerae Flos, Trichosanthis Radix showed high cytotoxity against G361, Rhei Radix et Rhizoma, Angelicae gigantis Radix, Gleditsiae Spina, Olibanum, Angelicae dahuricae Radix, Fritillariae cirrhosae Bulbus, Paeoniae Radix Rubra, Glycyrrhizae Radix, Ledebouriellae Radix, Myrrha against B16F10, Rhei Radix et Rhizoma, Manitis Squama against MDA, Citri Pericarpium, Manitis Squama against A549.

• 생약학회지
• /
• 제29권4호
• /
• pp.402-412
• /
• 1998

This study was attempted to investigate the effect of 'Angelicae gigantis Radix extract (AG.EX.)' and 'Angelicae acutilobae Radix extract (AA.EX.)' on the activities of GOT, GPT and alkaline phosphatase (A1.P), the contents of total cholesterol in serum of $CCl_4$ and D-galactosamine intoxicated rats, and the weight change ratio of body, liver and spleen in $CCl_4-intoxicated-rats$ by administering the extract of 300 and 500 mg/kg P.O.. Significant test was performed by comparision with the biochemical values between intoxicated-control group and extract-administered group. The activities of s-GOT, s-GPT and the contents of total cholesterol elevated by $CCl_4-intoxication$ were significantly decreased in all dose (300, 500 mg/kg) of Angelicae gigantis Radix-water extract (AG.WEX.) and alcohol extract (AG.AEX), and Angelica acutilobae Radix-water extract (AA.WEX.) and alcohol extract (AA.AEX.), respectively, as compared with the control group. And administered group of 300 mg/kg showed more significant decreasing effect than 500 mg/kg, and more significantly decreased in water extract of AG.EX. and ethanol extract of AA.EX. But in the activities of s-A1.P. inhibition effect were significantly decreased only in a dose of 300 mg/kg of AA.WEX. and AA.AEX. The activities of s-GOT and s-GPT elevated by D-galactosamine were not decreased in all samples, as compared to intoxicated-control group. But the activities of s-Al.p was significantly decreased as compared with control groups, in all samples and administration of 300 mg/kg was more significantly decreased than 500 mg/kg. The contents of total cholesterol remarkably decreased than the normal groups by D-galactosamine intoxication was not recovered in all samples. The increasing rate of the body weight increased by $CCl_4-intoxication$ were not decreased than the $CCl_4-control$ group in all sample groups. The increasing rate of liver weight increased by $CCl_4-intoxication$ were significantly decreased in 300 mg/kg of AA.AEX.AG.WEX. and AA.WEX., respectively, as compared with $CCl_4-control$ group.

• Food Science and Biotechnology
• /
• 제14권4호
• /
• pp.537-539
• /
• 2005

Potential of mass spectrometer-based electronic nose to discriminate habitat of Angelicae gigantis radix was evaluated using 24 and 86 Korean and non-Korean samples, respectively. Loading plot(s) of principal component analysis of data measured through this system revealed difference between Korean samples (probability; 100%) and non-Korean ones (probability; 95.3%), suggesting this technique could be used as efficient method to differentiate habitat of A. gigantis radix.

• Natural Product Sciences
• /
• 제10권6호
• /
• pp.262-267
• /
• 2004

A reversed-phase liquid chromatographic method for decursin and decursinol angelate of Angelicae gigantis Radix, an important crude drug in Korean traditional medicine, was developed and validated. Decursin and decursinol angelate, the structure isomer (pyranocoumarin) each other, are the main organic constituents in Angelicae gigantis Radix. This method was developed using a RP-18 column, UV detector at 280 nm and 50% acetonitrile solution containing 0.01 M sodium dodecyl sulfate and 25 mM sodium dihydrogen phosphate (pH 5.0) as the mobile phase. Various validation parameters were included and evaluated satisfactorily. Linearity was established in range 2-75 mg/ml of decursin and decursinol angelate (correlation coefficient = 0.9997 and 0.9995, respectively). This analytical method showed good accuracy (98.1% and 99.5%, respectively). Precision (repeatability) revealed a relative standard deviation value of 1.71% (decursin) and 3.19% (decursinol angelate). For intermediate precision measure the considered variables were equipment and days. A robustness test showing the influence of deferent counter-ion concentration in mobile phase was also performed.

• Natural Product Sciences
• /
• 제7권2호
• /
• pp.53-59
• /
• 2001

The possibility of Chungpesagan-tang, which has been recommended on the stroke patients with constipation in Korean traditional clinic, and its ingredients as a novel antithrombotic agent was evaluated. Most of its ingredients except Puerariae Radix exhibited in vitro antiplatelet aggregation activity. However, Puerariae Radix was significantly effective on ex vivo anti-platelet aggregation activity, whereas Angelicae Tenuissimae Radix, Raphani Semen and Angelicae Dahuricae Radix was not effective. Plasma recalcification was potently inhibited only by Puerariae Radix and Rhei Rhizoma treated with intestinal bacteria. Urokinase was also activated only by Chungpesagan-tang, Angelicae Tenuissimae Radix and Puerariae Radix treated with intestinal bacteria. Chungpesagan-tang exhibited the potent anti-thromboembolic activity activity in vitro. These results suggest that anti-thrombotic activity of Chungpesagan-tang should be activated by intestinal bacteria and may be important in the prevention of thrombosis and cardiovascular diseases, such as myocardial infraction stroke and arteriosclerosis.

• 한국작물학회지
• /
• 제38권1호
• /
• pp.60-65
• /
• 1993

7개 재배지역산 참당귀의 decursin 함량변이를 조사하였으며, 각 재배지의 기상요인들과의 상관분석을 통하여 기상환경이 당귀 근의 decursin 함량에 미치는 영향을 파악하고자 수행된 실험결과는 다음과 같다. 1. 참당귀 근의 유효성분인 decursin과 decursinol angelate의 정량은 순상컬럼을 사용한 HPLC법이나 GC법이 양호하였다. 2. 참당귀 뿌리의 decursin 함량은 봉화산 4. 86%, 영천산 4.75%이며 수원산은 2.33%로 재배지역에 따른 함량변이가 비교적 컸다. 3. 생육기간별 각 기상요인들과 decursin 함량과의 상관분석 결과 통계적 유의상관은 보이지 않았으나, 평균기온과 강수량은 대체적으로 부의 상관을 보였으며 일교차, 일사량, 일조시수등은 정의 상관을 나타냈다.

• 대한한방내과학회지
• /
• 제21권1호
• /
• pp.23-30
• /
• 2000

Objectives : To analyze the effects of Samul-Tang and combinations of constituent herbs on arterial contraction. Method : In order to investigate the effects, Angelicae gigantis Radix, Cnidii Rhizoma, Rehmanniae Radix, Paeoniae Radix, in which one of them, three of them, and all of them, were used to exam. Results : Samul-Tang significantly inhibited the contraction of artery induced by PE(phenylephrine), accordingly as the concentration of Samul-Tang increased and inhibited in both with intact and removed endothelium. Among the constituent herbs of Samul-Tang, Paeoniae Radix and Paeoniae Radix-Cnidii Rhizoma combination inhibited the PE-induced contraction of artery the most. Conclusions : Samul-Tang and constituent herbs of Samul-Tang inhibit the contraction of artery.

• 한국자원식물학회지
• /
• 제14권1호
• /
• pp.24-31
• /
• 2001

천궁에서는 butylidene phthalide, butyl phthalide, senkyunolide, cnidilide + neocnidilide 및 ligustilide가 동정되었으며, 이들 성분의 70% 이상을 ligustilide와 senkyunolide가 차지하였다. Phthalide류 총량으로 볼 때 연길산 및 안국산의 중국재배 천궁은 한국재배종보다 약간 높은 경향이었으나, 길림산은 한국 재배종보다 뒤떨어지는 것으로 나타났으며 각 성분의 함량도 낮아 품질이 나쁜 것으로 확인되었다. 또한, 중국의 연길산 천궁은 ligustilide와 senkyunolide의 광분해생성물인 butylidene phthalide 및 butyl phthalide함량이 높아 유통기간과 보관상의 문제가 있었다고 판단되어져 약재 수입시 유의하여야 할 것으로 사료된다. 당귀에서는 butylidene phthalide와 ligustilide가 동정 되었으며, 전체의 약 90%를 ligustilide가 차지하였다. 우리나라 재배종 당귀 중의 ligustilide 함량은 중국 연길산의 상품 및 하품보다 높았으나, 안국산 및 길림산보다는 낮았다. 그러나 길림산을 제외한 중국산 당귀는 ligustilide의 광분해산물로 생성되는 butylidene phthalide함량이 우리 나라 재배종에 비하여 훨씬 높은 것으로 나타나, 천궁에서와 같이 중국산 약재의 유통과 보관상의 문제점이 제기되었다.

• 대한본초학회지
• /
• 제28권5호
• /
• pp.39-44
• /
• 2013

Objectives : Allergy is an immune dysfunction caused by degranulation from mast cells in the early phase of allergic disease. The purpose of this study was to investigate the anti-allergic effect of fermented Angelicae gigantis Radix in human mast cell line, HMC-1. Method : The Angelicae gigantis Radix was fermented by Lactobacillus acidophilus. The cell toxicity of fermented Angelicae gigantis Radix(FAGR) was determined by MTT assay. The release of ${\beta}$-hexosaminidase from HMC-1 stimulated by phorbol-12-myristate 13-acetate (PMA) plus A23187 was determined by ${\beta}$-hexosaminidase assay. Also, the concentrations of cytokines (interleukin-$1{\beta}$, -6, -8 and tumor necrosis factor-alpha) were measured by enzyme-linked immunosorbent assay. The gene expression of COX-2 from HMC-1 stimulated by phorbol-12-myristate 13-acetate (PMA) plus A23187 was determined by reverse transcription polymerase chain reaction. The release of histamine on substance P-stimulated HMC-1 was measured by histamine assay. Result : The FAGR suppressed the release of ${\beta}$-hexosaminidase, a marker of degranulation, from HMC-1 stimulated by PMA plus A23187. The FAGR inhibited the production of interleukin-$1{\beta}$, -6, -8 and tumor necrosis factor-alpha. The FAGR inhibited the expression of COX-2 mRNA. The FAGR suppressed the release of histamine on substance P-stimulated HMC-1. Conclusion : These results provide that FAGR may be beneficial in the treatment of allergic inflammatory disease.