• Korean Journal of Medicinal Crop Science
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• v.7 no.4
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• pp.288-295
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• 1999

This experiment was conducted to evaluate the appropriate nutrient solution for hydroponics in Angelica keiskei. The nutrient solutions used were Korean solution, Yamazaki solution, Netherlands solution and open field cultivation. The highest yield of Angelica keiskei was 4, 542kg/10a at Netherlands solution. 'The yield at Netherlands solution was increased about 139 percent, compared to that at open field cultivation. The highest germanium contents was 0. 35ppm at Korean solution. The contents increased 40 percent at Korean solution compare to that at open field cultivation.

• Korean Journal of Medicinal Crop Science
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• v.8 no.1
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• pp.29-40
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• 2000

This experiment was conducted to find appropriate media in hydroponic Culture of Angelica keiskei. The mediaused were carbonized rice hull, perlite, vermiculite, mixture(carbonized rice hull (1) : perlite (1) : peatmoss (1)), mixture+peatmoss20%, mixture+peatmoss40%, mixtrue+peatmoss60%, perlite 40%+peatmoss40%+vermiculite20% and open field cultivation. The highest yield of Angeli ca keiskei was shown 4,428ka/10a at vermiculite. The yield of vermiculite was increased about 133 percent compared to that of open field cultivation. The highest germanium contents was shown 0.52ppm at mixture+peatmoss 40%. The contents of germanium increased about 108 percent at mixture+peatmoss40% compared to that of open field cultivation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.12
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• pp.1846-1853
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• 2010

The storage attributes of green juice prepared by washing Angelica keiskei with various washing solutions using air bubble method were investigated. The washing solutions were distilled water (DW), sodium hypochlorite electrolyzed water (SHEW), and slightly acidic electrolyzed water (SAEW). During storage at $4^{\circ}C$, the number of bacteria after 1 day was $>10^5$ CFU/mL when DW was used, whereas bacterial growth was $<10^5$ CFU/mL after 4 days when SHEW or SAEW was used. The pH and color were not changed, and the polyphenol content, electron donating ability and total antioxidant ability were decreased slowly with the increase of storage time. No significant difference in any of the measured properties was found among washing methods (p<0.05). Consequently, these results suggest the possible use of electrolyzed water for washing to enhance the shelf life of green juice with A. keiskei because SHEW and SAEW decreased bacterial growth without affecting other properties of the green juice.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.12
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• pp.1654-1661
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• 2011

We investigated the effect of Angelica keiskei ethanol (AKE) extract on cell death in MDA-MB-231 human breast cancer cells. MDA-MB-231 cells were cultured in the presence 125, 150 and 175 ${\mu}g$/mL concentrations of AKE for 24 hours. MTT assays demonstrated that mitochondrial dehydrogenase activities decreased in a dose-dependent manner in MDA-MB-231 cells (p<0.05). In contrast, the proportion of dual staining with Hoechst 33342/ethidium bromide(EtBr) for cell death increased in a dose-dependent manner in MDA-MB-231 cells (p<0.05). In particular, the levels of cell death caused by apoptotic program showed marked increases in the 150 and 175 ${\mu}g$/mL AKE groups, as revealed by flow cytometry. An apoptotic suppressor gene, Bcl-2, significantly decreased at the transcript level (p<0.05). The expression levels of proapoptotic genes, both Bax and caspase 3 significantly increased (p<0.05). Furthermore, the ratio of Bcl-2/Bax mRNA which is considered to be an important indicator of apoptosis, significantly decreased in a dose-dependent manner (p<0.05). These results taken together indicate that, the AKE extract used in this study induces cell death in MDA-MB-231 human breast cancer cells.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.7
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• pp.367-373
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• 2016

This study was performed to set a limitation rule to the UV sterilization process for green vegetable juice containing barley sprouts. The main constituents of the green vegetable juice, viz. barley sprouts, organic Angelica keiskei, carrots, organic dandelion, kale, wild parsley, Angelica keiskei and cabbage, were provided by the CSJ company located in Jincheon, Chungbuk, from December 1st, 2015 to March 1st, 2016. Our manufacturing process followed the general manufacturing process of a general green vegetable juice manufacturer. Table 1 shows the mixing ratio for the green vegetable juice. This green vegetable juice includes 18% vegetable sprouts, 3% barley sprouts, 18% angelica utilis makino, 1% orange concentrate, 4% apple concentrate, 6% fructooligosaccharide, 10% vegetable fermentation juice, 0.4% lemon concentrate and 39.6% purified water. We conducted UV sterilization at levels 10, 8 and 5 with powers of 230W, 320W and 320W, respectively. The UV sterilization was conducted twice at 25Hz and then once at 40Hz. In the bacterial tests after sterilization, the number of bacteria in the dandelions decreased from $2.56{\times}10^8CFU/mL$ to $5.96{\times}10^3CFU/mL$. In addition, no food poisoning or coliform bacteria were detected. Thus, this study provides the information required for setting a limitation rule using bacteria tests in the UV sterilization process for green vegetable juice.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.6
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• pp.1227-1236
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• 2001

Genotoxicological safety on 10 kGy-gamma irradiated vegetable juices such as Oenanthstolonifera DC., Daucus carota L., Brassica oleracea var. acephala and Angelica keiskei was determined by the Salmonella typhmurium reversion assay, the SOS Chromotest using in Escherichia cloi PQ37 and chromosome aberration test in cultured Chinese hamster lung (CHL) fibroblast cells. Vegetable juices exposed to 10 kGy-gamma ray revealed negative results in these three in vitro mutagenetic tests.

• Food Science and Preservation
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• v.11 no.3
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• pp.424-429
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• 2004

Proximate components, minerals, vitamins and chlorophyll contents were determined in vegetables purchased at Garak-dong market and organic vegetables cultivated at Hongchun, Kangwon-do. The vegetables were Kale, Angelica Keiskei Koidz, Celery, Lettuce and Allium fistulosum. In proximate component contents, analyzed crude protein and fat contents of organic vegetables were higher than those of general vegetables. Organic vegetables had strong green color. Minerals such as sodium, potassium, calcium, phosphorus, iron as well as vitamins were determined. Contents of minerals and vitamins in organic vegetables were higher than those of general vegetables. Total mineral content of organic and general kales were 811.8 mg/l00g, and 688.1 mg/l00g, respectively. Total mineral content of organic Angelica Keiskei Kowz, Celery, Lettuce and Allium fistulosum were 447.9, 486.5, 368.6, and 320.9 mg/100g, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.7
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• pp.1030-1037
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• 2010

A fresh juice has become a new functional food available for dieting and health. However, the shelf-life of vegetable juice is very short because of the absence of heat pasteurization process. To elongate the shelf-life of vegetable juices, such as Angelica keiskei and Brassica loeracea var. acephala, the changes of microbiological, chemical and sensory property by UV irradiation were investigated. The total aerobic bacterial numbers of A. keiskei and B. loeracea var. acephala vegetable juices were $3.2{\times}10^5$ and $7.0{\times}10^4\;CFU/mL$, respectively, after wring process. However, the numbers were $3.6{\times}10^3{\sim}9.7{\times}10^3$ and $3.7{\times}10^3{\sim}2.7{\times}10^4\;CFU/mL$ after UV treatment on wring juice, and this lower microbial number was maintained during storage. The number of coliform bacteria also reduced significantly by UV treatment, and the bactericidal effect was higher when the flow rate is slower. The increase of lightness and yellowness, and decrease of redness were observed after treatment of UV on both vegetable juices, but the differences were not significant between flow rates. The ascorbic acid contents of vegetable juices were reduced by UV irradiation regardless of flow rate, and storage. Overall acceptance in sensory analysis revealed that there was no significant difference between the control and vegetable juice irradiated UV at 0 days, but sample with UV treatment showed higher score at 3 days. Therefore, UV treatment on vegetable juice can elongate the shelf-life without any problems in flavor and color.

• Journal of the Korean Society of Food Culture
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• v.14 no.2
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• pp.167-175
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• 1999

This study examines the effects of the Korean horticultural foods which are, by traditional wisdom, supposed to contain anti-aging substance. All twenty three kinds of plants are chosen as a sample for the experiment in which the content of three main anti-aging nutrients, -carotene, Vitamin C, and Vitamin E are respectively measured by Nilis, Colorimetric, and AOAC method. The result shows: among the samples, top six plants that contain the most -carotene content are high quality tea(Camellia sinensis), thistle(Cirsium japonicum Var.), Angelica keiskei, mulberry leaves(Morus alba l.), field horsetail(Equisetum arvensa), and carrot(Daucus carota), in order; top four for a Vitamin C contents are low quality tea, mulberry leaves, pine leaves(Pinus densiflora), and high quality tea, in order; top four for a Vitamin E contents are persimmon leaves(Diospyros mongolicum) tea, high quality tea, low quality tea, and pine leaves, in order. Drying or boiling process increases the content of -carotene and Vitamin E in high quality tea, dropwort(Oenanthe javanica), field horsetail, and wild aster(Ligularia stenocephala Marsumum et Koldzumi), while, in case of mulberry leaves, drying process lowers them. Vitamin C content strikingly decreases in heating and drying process.

• Food Industry And Nutrition
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• v.8 no.3
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• pp.45-51
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• 2003

본 연구는 생물공학적인 방법을 도입하여 폐기되는 신선초박에 H. erinaceum의 균사체를 발효시킨 배양생성물을 이용하여 기능성 건강 음료 개발을 검토 하고자 하였다. 1. 종균제조공정개발 : 기본 배지의 선발에서 Hericium erinaceum의 균사 생육에 적합한 배지를 선발하기 위하여 10여종의 고체배지를 사용하여 균사 생육 및 밀도를 조사한 결과는 YMPG 배지에서 59.8mm/14days로 균사 생육이 가장 우수한 것으로 나타났고, 최적 온도는 20-$25^{\circ}C$ 범위에 가장 생육이 좋았으며, 배지의 pH를 조절하여 균사생육을 조사한 결과, pH는 5.5, 접종비는 전배양액 9%(v/v), 배양에 적합한 배지액량은 50mL, 최적교반속도는 120rpm이었다. 이러한 최적 조건 하에서 배양 경시 변화를 살펴 본 결과 당은 거의 일정한 속도로 감소하는 반면에 건조균체량은 배양 8일째까지 증가하다가 더 이상 변화가 없었다. 2. 발효공정개발: 수분함량이 200%(v/v)에서, pH5에서의 생육속도는 90mm/30 days, $25^{\circ}C$에서의 균사생육속도는 89mm/30days로 각각 H. erinaceum균사의 생육이 가장 우수한 결과를 얻었다 3.추출공정 및 시제품 제조: 녹즙을 생산한 후 폐기되는 신선초박에 액체 종균을 접종하여 ,40일 동안 배양시켜 생육 상태가 우수한 배양생성물만을 선별하여, 열수추출방법으로 10$0^{\circ}C$, 10시간 추출한 것을 음료제조의 원료로 하고, 음료의 기호성을 향상시키기 위해 유기산 및 한방추출물을 첨가하는 균사체음료의 조성비를 얻었다.