• Title/Summary/Keyword: Angelica keiskei

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Isolation and Characterization of Antioxidative Compounds from the Aerial Parts of Angelica keiskei

  • Kim, So-Joong;Cho, Jeong-Yong;Wee, Ji-Hyang;Jang, Mi-Young;Kim, Cheol;Rim, Yo-Sup;Shin, Soo-Cheol;Ma, Seung-Jin;Moon, Jae-Hak;Park, Keun-Hyung
    • Food Science and Biotechnology
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    • v.14 no.1
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    • pp.58-63
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    • 2005
  • Ethyl acetate-soluble neutral fraction of hot water extracts from the aerial parts of Angelica keiskei showed a 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity. Six antioxidative compounds were purified and isolated by various chromatographic procedures. Based on the analyses of FAB-MS and NMR, the isolated compounds were structurally elucidated as luteolin 7-O-${\beta}$-D-glucopyranoside (1), quercetin 3-O-${\beta}$-D-galactopyranoside (2), quercetin 3-O-${\beta}$-D-glucopyranoside (3), quercetin 3-O-${\alpha}$-D-arabinopyranoside (4), kaempferol 3-O-${\alpha}$-D-arabinopyranoside (5), and luteolin 7-O-rutinoside (6). The glycosides of flavonols and luteolin showed DPPH radical-scavenging activity. One molecule of 2, 3, 4, 6, 1, and 5 scavenged 4.2, 4.2, 4.1, 2.5, 2.2, and 1.4 molecules of DPPH radical, respectively.

Variation of Retrogradation and Preference of Bread with Added Flour of Angelica keiskei Koidz during the Storage (신선초가루를 첨가한 식빵의 저장 중 노화도와 기호도의 변화)

  • 최옥자;정현숙;고무석;김용두;강성두;이홍철
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.1
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    • pp.126-131
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    • 1999
  • We have got the following results from tests in the course of time retrogradation and taste change in bread with added flour of Angelica keiskei Koidz, which had been stored in room temperature (27±2oC), refrigerating(2±1oC) and freezing( 20±1oC), respectively. Bread with the added flour showed a little lower degree of retrogradation than control group, and every group in room temperature retrograded from the very first day. The degree of retrogradation of the group in refrigerating was far greater comparing with the group in room temperature. The longer bread was stored, the lower the degree of retrogradation was, and the higher the adding rate of the flour got, the later its retrogradation happened. Especially the flour of this vegetable's stem turned out to be the most effective in retarding its retrogradation. Group stored 30 days in freezing didn't show a wide difference in retrograde comparing with the group before storage. Preference for color and shape of the flour added bread stored in room temperatue, refrigerating and freezing did not change significantly from the group before storage. Although preference decreased for flavor, texture, mouth feel and overall quality with the lapse of time, flour added group improved in preference for these factors comparing with the control group. Above all, added flour of pretreated stem proved to be the most effective in the sensory tests. With respect to the storing method, the group stored in refrigerating showed greater preference for the texture and mouth feel over the groups in room temperature and freezing. Flavor preference of the group in freezing was the lowest of all.

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Isolation and Identification of Two Psoralen Derivatives as Antioxidative Compounds from the Aerial Parts of Angelica keiskei (신선초에 함유된 항산화활성물질 Psoralen 유도체들의 단리 및 동정)

  • Kim, So-Joong;Cho, Jeong-Yong;Wee, Ji-Hyang;Jang, Mi-Young;Rim, Yo-Sup;Kim, Cheol;Shin, Soo-Cheol;Moon, Jae-Hak;Park, Keun-Hyung
    • Korean Journal of Food Science and Technology
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    • v.37 no.4
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    • pp.656-659
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    • 2005
  • Hot water extracts of Angelica keiskei aerial parts were solvent-fractionated with ethyl acetate (EtOAc) and buffers (5% $NaHCO_3$, pH 8.0; 1.0 N HCl, pH 3.0) to obtain EtOAc-soluble acidic and neutral fractions. EtOAc-soluble neutral fraction was purified by Sephadex LH-20 column chromatography and reverse phase HPLC. Assay for purification of antioxidative compounds was performed by spraying DPPH solution on thin layer chromatography. Two isolated substances were identified as pseudoisopsoralen and 8-methoxypsoralen(xanthotoxin) by FAB-MS and NMR analyses.

Angelica keiskei Improved Beta-amyloid-induced Memory Deficiency of Alzheimer's Disease (아밀로이드 베타로 유발한 알츠하이머병 모델에서 신선초의 기억력 개선 효과)

  • Lee, Jihye;Kim, Hye-Jeong;Kim, Dong-Hyun;Shin, Bum Young;Jung, Ji Wook
    • The Korea Journal of Herbology
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    • v.34 no.3
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    • pp.1-7
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    • 2019
  • Objectives : Amyloid ${\beta}(A{\beta})$ could induce cognitive deficits through oxidative stress, inflammation, and neuron death in Alzheimer's disease (AD). This study was investigated the effect of Angelica keiskei KOIDZUMI (AK) on memory in $A{\beta}$-induced an AD model. Methods : AK was extracted uses 70% ethanol solvent. Total polyphenol and flavonoids content were obtained by the Folin-Ciocalteu and the Ethylene glycol colorimetric methods, respectively. The antioxidant activities were assessed through free radical scavenging assays using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) methods. Intracerebroventrical (i.c.v) injection of $A{\beta}$ 1-42 was used to induce AD in male ICR mice, followed by administrations of 5, 10 or 20 mg/kg AK on a daily. Animals were subjected to short and long term memory behavior in Y-maze and passive avoidance test. Results : The total polyphenol and flavonoids contents of the AK extract were $88.73{\pm}6.36mg$ gallic acid equivalent/g, $84.21{\pm}5.04mg$ rutin equivalent/g, respectively. The assays of DPPH and ABTS revealed that AK extract in treated concentrations (31.25, 62.5, 125, 250, 500, $1000{\mu}g/m{\ell}$) increased antioxidant activity in a dose-dependent manner. Oral administration of AK extract significantly reversed the $A{\beta}$ 1-42-induced decreasing of the spontaneous alternation in the Y-maze test and $A{\beta}$ 1-42-induced shorting of the step-through latency in the passive avoidance test. Conclusions : The findings suggest that AK indicated the antioxidant protective effects against $A{\beta}$-induced memory deficits, and therefore a potential lead natural therapeutic drug or agent for AD.

Effect of Peel and Whole Crop of Kale and Angelica Keiskei Koidz on Fatty Acid Composition and Quality of Eggs (케일과 명일엽 및 부산물의 급여가 계란 품질 및 지방산 조성에 미치는 영향)

  • Kang, Hwan-Ku;Kang, Geun-Ho;Kim, Dong-Wook;Lee, Sang-Jin;Kim, Sang-Ho
    • Food Science of Animal Resources
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    • v.28 no.5
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    • pp.645-650
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    • 2008
  • The effects of various levels of dietary dried whole crop of kale (0.1%, 0.3%), dried peel of kale (0.3%), dried whole crop of Angelica keiskei (Koidz) (0.1%, 0.3%), and peel of Angelica keiskei (Koidz) (0.3%) in egg-laying performance were studied with 560 ISA brown layers for a period of 12 weeks. No significant differences were recognized between the treatments and control in egg production, feed intake and egg mass. The quality of egg and eggshell were not different among treatments but egg yolk color was improved in the both of whole crop and peel of kale and Angelica keiskei (Koidz). Vitamin and polyunsaturated fatty acid concentration of eggs increased in treatments compared to control but were not significantly different from controls. In conclusion, dried peel of kale and Angelica keiskei (Koidz) supplementation in laying hens diet improved egg yolk color, and improve vitamin concentration of chicken eggs.

Antitumor Activity of Methylene Chloride Fraction from Angelica Keiskei Through Induction of Apoptosis in Human Prostate Carcinoma DU145 Cells (전립선 암세포주 DU145의 세포고사 유도를 통한 신선초 (Angelica keiskei) 메틸렌 클로라이드 추출물의 항암효과)

  • Kang, Yoon-Mook;Kim, Sung-Moo;Kim, Hyun-Jung;Park, Kyung-Ran;Shim, Bum-Sang;Kim, Sung-Hoon;Choi, Seung-Hoon;Ahn, Kyoo-Seok;Ahn, Kwang-Seok
    • Journal of Korean Traditional Oncology
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    • v.15 no.1
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    • pp.19-27
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    • 2010
  • The roots and leaves of Angelica keiskei (AK) have been used for the treatment of various diseases including coronary heartdisease, hypertension, and cancer in the Korean folk medicine. However, the mechanism by which methylenechloride fraction (MF) from AK exerts anti-tumorigenic activity in human prostate cancer cells has not been fully understood. In the present study, we report the MF exerted the highest cytotoxicity against prostate cancer DU145 cells compared with other fractions. Especially, MF caused the accumulation of sub-G1 DNA contents of cell cycle and increased annexin V-positive apoptotic bodies and DNA fragmentation. MF down-regulated several proliferative (Cyclin D1) and anti-apoptotic (Bcl-xl, Bcl-2, IAP-1/2, and survivin)gene products in these cells. Hence, MF induced apoptosis through the caspase-3 activation in DU145 cells. We further confirmed that caspase-3 plays an importance role in MF-induced apoptosis in DU145 cells by using caspase-3 inhibitor. Additionally, we observed that MF potentiated Dox-induced apoptosis in DU145 cells. Taken together, our data demonstrate the evidence that MF induces apoptosis depend on caspase-3 activation of and overcomes resistance to chemotherapy in human prostate cancer cells.

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Analysis of Antioxidant Nutrients in Green Yellow Vegetable Juice (녹즙의 항산화 영양성분 분석)

  • Chung, So-Yong;Kim, Hyeon-Wee;Yoon, Sun
    • Korean Journal of Food Science and Technology
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    • v.31 no.4
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    • pp.880-886
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    • 1999
  • The present study was attempted to investigate the contents of antioxidant nutrients in green juice of Angelica keiskei, kale, carrot, celery, cucumber. All 5 green juice were found to contain antioxidant nutrients such as ${\beta}-carotene$, vitamin E, vitamin C, Se, Cu, Mn, Zn and phenols. ${\beta}-carotene$ contents of carrot, kale, Angelica keiskei, celery and cucumber juice were 5909.0, 3285.3, 1398.4, 176.7 and 61.4 mg/l00 g, respectively. ${\alpha}-tocopherol$ contents of 5 green juices were $0.04{\sim}1.41$ mg/l00 g. Kale juice had highest ${\alpha}-tocopherol$ and celery juice contained lowest, kale juice also contained highest vitamin C (105.1 mg/l00 g) and Angelica keiskei, cucumber, celery and carrot had vitamin C contents of 31.4, 9.3, 7.5 and 5.2 mg/l00 g, respectively. Angelica keiskei had highest amount of antioxidant minerals among the sample such as Cu (0.40 mg/l00 g), Mn (0.5 mg/l00 g), Zn (0.24 mg/l00 g), Se $(0.61\;{\mu}g/kg)$. Kale was the second best source of antioxidant minerals which were Cu (0.45 mg/l00 g), Mn (0.22 mg/l00 g), Zn (0.24 mg/l00 g), Se $(0.33\;{\mu}g/kg)$. However, celery and cucumber were poor in antioxidant minerals. Total phenolic contents of 5 green juices were $0.01\;{\sim}0.05%$, kale juice also had highest total phenolics among the samples.

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Changes in Antioxidant and Nitrite Scavenging Activities of Angelica keiskei and Brassica loeracea var. acephala Vegetable Juices Treated with UV Irradiation during Storage (UV 조사한 신립초 및 케일 녹즙의 항산화 활성 및 아질산염 소거작용의 변화)

  • Choi, Goo-Hee;Kwon, Sang-Chul;Lee, Kyung-Haeng
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.8
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    • pp.1187-1193
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    • 2010
  • To elongate the shelf-life of Angelica keiskei and Brassica loeracea var. acephala vegetable juices, UV irradiation was used and the changes of antioxidant activity and nitrite scavenging ability were investigated. The content of polyphenols of vegetable juices were slightly reduced by UV treatment and/or storage period. The DPPH radical-scavenging activities of the vegetable juices treated by UV were higher than that of control but were not changed during storage. However, $ABTS{\cdot}^+$ reducing activities of the vegetable juices were reduced by UV treatment. The $ABTS{\cdot}^+$ reducing activity of Brassica loeracea var. acephala juice was lower when the flow rate was slower. The ferrous ion chelating effects of Angelica keiskei vegetable juices were reduced by UV treatment. In contrast, the ferrous ion chelating effects of Brassica loeracea var. acephala vegetable juices were not different from those of right after manufacturing. The ferrous ion chelating effects on both vegetable juices increased during storage periods. The inhibitory activity of lipid oxidation was decreased slightly by UV treatment on vegetable juices. The nitrite scavenging ability of Angelica keiskei and Brassica loeracea var. acephala vegetable juices treated by UV irradiation was not different from that of control. The nitrate scavenging abilities of vegetable juices in pH 1.2 were higher than those in pH 3.0 and 4.2.

Growth and Yield in different Hydroponic Solutions of Angelica keiskei Koidzumi (양액종류(養液種類)에 따른 신선초(神仙草)의 생육(生育) 및 수량(收量))

  • Han, Seoung-Ho;Choi, Byung-Jun;Han, Kwang-Seop;Shin, Cheol-Woo;Moon, Chang-Sik;Seo, Sang-Duck;Chang, Ki-Woon;Park, Sang-Il
    • Korean Journal of Medicinal Crop Science
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    • v.7 no.4
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    • pp.288-295
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    • 1999
  • This experiment was conducted to evaluate the appropriate nutrient solution for hydroponics in Angelica keiskei. The nutrient solutions used were Korean solution, Yamazaki solution, Netherlands solution and open field cultivation. The highest yield of Angelica keiskei was 4, 542kg/10a at Netherlands solution. 'The yield at Netherlands solution was increased about 139 percent, compared to that at open field cultivation. The highest germanium contents was 0. 35ppm at Korean solution. The contents increased 40 percent at Korean solution compare to that at open field cultivation.

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Effects of Angelica keiskei Koidzumi and Turmeric Extract Supplementation on Serum Lipid Parameters in Hypercholesterolemic Diet or P-407-Induced Hyperlipidemic Rats (명일엽과 울금 추출물의 투여가 고콜레스테롤식이와 P-407로 유도한 고지혈증쥐의 혈중 지질 함량에 미치는 영향)

  • Kim, Tae-Hyun;Son, Yeon-Kyung;Hwang, Keum-Hee;Kim, Mi-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.6
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    • pp.708-713
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    • 2008
  • The effects of administration alone or mixed of Angelica keiskei and turmeric extract on blood lipids were evaluated in hypercholesterolemic diet or P-407-induced hyperlipidemic rats. In the study 1, female Sprague-Dawley rats weighing 160-180 g were divided into each groups and fed high cholesterol diets for 8 weeks. Experimental groups were administered with following diets: basal diet (Normal), high cholesterol diets (1% cholesterol). We did the oral administration for evaluation in experimental groups: C (vehicle), A (angelica extract), T (turmeric extract), AT (angelica extract, turmeric extract/ 1:1 complex). The concentrations of serum total cholesterol, and LDL-cholesterol were decreased by 6.8%, 9.8% in A group, by 22.1%, 28.8% in C group and by 28.2%, 35.6% in AT group, compared to the C group, respectively. HDL-cholesterol levels were not different among the experimental groups. In the study 2, we induced the hypertriglyceridemia in rats by intraperitoneal injection of P-407 (0.5 g/kg) once per three days. From the next day after P-407 injection beginning, we did the oral administration as the study 1. Angelica keiskei extract, turmeric extract and complex extract decreased serum triglyceride by 17.2%, 19.7% and 48.3%, respectively. These results suggested that Angelica keiskei and turmeric extract complex might have synergistic effect in lowering total cholesterol, LDL-cholesterol and triglyceride in hyperlipidemic rats.