• 대한한방소아과학회지
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• 제34권2호
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• pp.57-74
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• 2020

Objectives The purpose of this study is to analyze clinical studies on the efficacy and safety of herbal medicine in vitiligo by analyzing randomized controlled trials (RCTs). Methods Three electronic databases including the PubMed, EMBASE, and Cochrane library were used to search for randomized controlled trials, by using specific key words and criteria up to January 4^th, 2020. Data in regards to years of publication, nation, demographic information, disease characteristics, duration of diseases, treatment methods, treatment period, outcome measures, results and adverse events were collected for this study. Results A total of 11 randomized controlled trials were selected and analyzed. The total effective rate of the treatment group treated with herbal medicine was significantly higher than that of the control group. In the other outcome measures, the treatment group also showed statistically significant differences in improving the outcome measures compared to the control group, or showed similar treatment effects to the control group. The most commonly used herbal medicines were Carthami Flos (紅花), Angelicae Gigantis Radix (當歸), Angelica dahurica Bentham et Hooker f. (白芷), Astragali Radix (黃芪), Glycyrrhizae Radix et Rhizoma (甘草), Salviae Miltiorrhizae Radix (丹參), Persicae Semen (桃仁), Araliae Continentalis Radix (獨活), Tribuli Fructus (白蒺藜), Psoraleae Semen (補骨脂) etc. Hardly any severe adverse events were reported from the trials selected. Conclusions Based on the results of the clinical studies, herbal medicine treatment could be an effective and safe option for vitiligo treatment and symptom improvement.

• 사상체질의학회지
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• 제25권3호
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• pp.208-217
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• 2013

Objectives Modified Yeolda-Hanso tang (MYH) is a traditional herbal formula in Korea for various diseases. MYH is containing the 10 herbs : Pueraria lobata (Willd.) Ohwi, Angelica tenuissima Nakai, Scutellaria baicalensis Georgi, Platycodon grandiflorum (Jacq), Angelicae Dahurica, Cimicifuga heracleifolia Kom, Raphanus sativa L., Polygala tenuifolia (Willd), Acorus gramineus Soland and Dimocarpus longan Lour. The 10 herbs is constituted as a ratio of the 6:4:2:1:2:2:2:4:6:6. We investigated neuroprotective effects of MYH on human neuroblastoma SH-SY5Y cells and evaluated the ability of MYH to prevent and treat for neurodegenerative diseases such as Parkinson's disease via basal autophagy enhancement. Methods Pharmacological induction of Autophagy by MYH in SH-SY5Y cells: Induction of autophagy by MYH in human neuroblastoma SH-SY5Y cells was carreid out by immunoblot analysis with several autophagy markers. SH-SY5Y cells were treated with MYH at the concentration of 400 and $800{\mu}g/ml$ for 24 hr. Specifically, the autophagosome proteins LC3 II and Atg5 levels were increased and autophagy pathway related proteins such as beclin-1, PI3 Kinase class III protein, ULK1, mTOR and AMPK were activated. Conclusions MYH can enhance the induction of autophagy through key regulator AMPK, mTOR, and Beclin-1 and it should be considered as a possible candidate of neuroprotective agents for such as Parkinson's disease.

• 한방안이비인후피부과학회지
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• 제15권2호
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• pp.80-103
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• 2002

To screen the effective materials for hair loss treatment, several natural extracts were tested using in vivo and in vitro test models. Firstly, all test materials were applicated onto the back skin of C57BL／6 mouse and then hair growth pormoting effect were measured using hair growth index As a result, Polygonum muitifiorum Thunb and Terrninalia chebula Retz. showed potent hair growth promoting effect, ranking as 1.5-2.0 of hair growth index. However, there were no plant extracts, which have remarkable potential of growth promotion of human hair dermal papilla cells cultured in vitro. In the experiments of 5${\alpha}$-reductase type Ⅱ inhibition assay, Morus alba L., Chaenomelis Fructus, Saussureae Radix, Angelicae Gigantis Radix, Polygonum multifiorum Thunb, and Angelica dahurica (Fischer) Bentham et Hooker f. showed effective potential to inhibit the activity of 5${\alpha}$-reductase type Ⅱ. To investigate the possible involvement of effects of several plant extracts on the gene expression of growth factors in human hair dermal papilla cells, RT-PCR analyses were performed. As a consequences, Mentha haplocalyx Briq., Cimicifuga foetida L., Eclipta prostrata (L.) L., Pinus densiflora S. et. Z, and Polygonum muitifiorum Thunb revealed the regulatory roles on the expression of growth factors such as IGF-I, KGF, HGF and VEGF in the dermal papilla cells. Another test for inhibition of microbial such as P. acne and P. ovale were also carried out to find whether these plant extracts have anti-microbial activities. Morus alba L. and Chaenomelis Fructus showed anti-microbial effects on Propionibacterium acnes, which is believed as a pathogen of acne. Together, these results showed several plant extracts can be used for hair growth promotion.

• 한국자원식물학회지
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• 제10권1호
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• pp.100-104
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• 1997

백지(白芷) 1호(號)는 1990년(年)에서 1995년(年)까지 5개년간(個年間) 전국(全國)에서 수집(蒐集)한 13개(個) 백지재래종(白芷在來種) 중(中) 영주종(榮州種)을 순계분리(純系分離) 한 후(後) 생산역검정시험(生産力檢定試驗), 지성적응시험(地城適應試驗) 및 농가실증시험(農家實證試驗) 결과(結果) 다수성(多收性) 계통(系統)임이 확인(確認)되어 1995년(年) 농촌진흥원(農村振興聽) 종자심요회(種子審議會)에서 우량품종(優良品種)으로 결정(決定)되었기에 육성과정(育成過程)에서 나타난 주요특성(主要特性)과 수량성(收量性)을 요약(要約)하면 다음과 같다. 1. 백지(白芷) 1호(號)는 근피색깔이 황갈색(黃褐色)이고 직근(直根)이며 장원추형(長圓錐形)으로 봉화종(奉化種)보다 우수(優秀)하여 양질(良質)의 생약재(生藥材) 생산(生産)에 유리 한 품종(品種)이었다. 2. 백지(白芷) 1호(號)는 초장(草長), 근장(根長) 및 근직경(根直徑)은 봉화재래종(奉化在來種) 보다 컸으나 출현기(出現期), 개화기(開化期), 엽수(葉數)는 비 슷하였다. 3. 백지(白芷) 1호(號)는 근중(根中) 엑스 함량(含量)과 회분(灰分) 및 산불용성(酸不用性) 회분(灰分) 함량(含量)으로 보아 대한약전(大韓藥典) 규격한(規格韓)에 규정(規定)된 함량치(含量置)에 적합(適合)하여 생약(生藥)으로 이용시(利用時) 안전성(安全性)과 유효성(有效性) 갖추었다. 4. 백지(白芷) 1호(號)는 내토양선충성(耐土壤線蟲性) 및 내서성(耐署性)이 봉화종(奉化種) 보다 강한 편이었고 탄저병(炭저病), 도복(倒伏), 내습성(耐濕性)은 봉화종(奉化種)과 비슷하였다. 5. 백지(白芷) 1호(號)의 건근수량성(乾根收量性)은 생산력검정시험(生産力檢定試驗), 지역적응시험(地域適應試驗) 및 농가실증시험(農家實證試驗) 결과(結果) 모두 봉화종(奉化種)보다 21-31% 증수(增收)하였다.\sim}6%$로 생각된다. 4. 1년생(年生) 사초(飼草)인 수단그라스계(系) 잡종(雜種)에 있어서 재생(再生)에 기여하는 저장탄수화물(貯藏炭水化物)의 역할은 뚜렷하지 못하며, 예취시(刈取時) 기상조건(氣象條件) 등 다른 요인(要因)들이 재생수량(再生收量)에 더 많은 영향을 미치는 것으로 생각된다.지(4.5%), 자연자원(3.9%), 수질 (3.9%), 국립공원(3.4%) 등의 순으로 나타나 환경교육에서 다양한 환경대상에 다양한 관심을 가지고 있음을 알 수 있다. 이들 결과로 부터 외국에서 수행원 환경교육 연구의 종류와 동향을 알 수 있으며, 이는 우리나라 환경교육 연구의 방향성을 설정하는데 도움을 줄 수 있을 것으로 생각된다.등전점(登電點) 전기영동법(電氣泳動法)으로 esterase와 peroxidase 동위효소(同位酵素) 특성(特性)을, 그리고 SDS-PAGE법(法)으로 단백질(蛋白質) 양상을 등숙시기별(等熟時期別)로 조사(調査)한 결과(結果) 두 계통간(系統間) 질적(質的)인 차이(差異)는 보이지 않았으나 시기별(時期別)로는 양적차이(量的差異)를 나타냈다.間)에는 부(負)(-)의 상관(相關)이 있다.($P{\leq}0.01%$). 5. NEL 및 starch value 환경온도(環境溫度)가 상승(上昇)됨에 따라 감소(減少)된다. 4 엽기(葉期) sorghum식물(植物)의 환경온도(環境溫度)를 달리 하였을 때 NEL가치(價値)는 각각(各各) 4.87MJ($30/25^{\circ}C$), 5.46MJ($25/20^{\circ}C$) 및 5.81MJ/kg($18/8^{\circ}C$)로 변(變)하여 고온(高溫)에서 net energy lactation 축적(蓄積)이 크게 감소(減少)되었다.다. 그러나 기온(氣溫)이 낮은 조건(條件)에서는 예취관리(刈取管理)

• Journal of Nutrition and Health
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• 제57권1호
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• pp.53-64
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• 2024

본 연구는 근생성 효능을 가진 천연화합물을 발굴하고자 oxypeucedanin hydrate가 근생성과 미토콘드리아에 미치는 영향 및 항암제 유도 미토콘드리아 손상에 대한 완화효과를 C2C12 근원세포와 zebrafish 모델을 통해 각각 확인하였다. 그 결과, oxypeucedanin hydrate는 다핵의 근관세포의 수와 분화말기 표지자인 Myh4의 발현량을 증가시켰고 근육단백질 분해 인자인 MuRF1과 MAFbx의 발현량은 감소시켰다. 또한 미토콘드리아 생합성 조절인자인 Pgc1α, Tfam과 전자전달계 구성인자인 Sdha, Cox1의 발현은 증가시키고, 미토콘드리아 융합인자인 Opa1의 발현 또한 증가시킴과 동시에 미토콘드리아 분열을 표지하는 Drp1의 발현은 감소시켰다. 한편 zebrafish 모델을 통해 항암제 유도 미토콘드리아 손상에 대한 개선효과를 확인한 결과, oxypeucedanin hydrate는 항암제에 의해 유도된 미토콘드리아 손상을 완화시켰다. 이상의 결과들은 oxypeucedanin hydrate가 미토콘드리아 기능 증진을 매개로 근원세포 분화 촉진 및 근육 단백질 분해 저하 효과를 나타냄을 시사한다. 따라서 본 연구를 통해 oxypeucedanin hydrate가 근생성 효과를 나타낼 수 있는 잠재적인 유효소재로서의 가능성을 제시하였다.

• 한방안이비인후피부과학회지
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• 제16권1호
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• pp.42-62
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• 2003

Subjects : We tried to analysis dermatosurgical prescriptions including 477 WonBang(元方) prescriptions for SangJungHaTong(上中下統) introduced by HwangDoYeon(黃道淵). Methods : Prescriptions in BangYakHapPyun(方藥合編) is generally categorized into SangTong(上統), JungTong(中統), HaTong(下統) which are called PoJe(補劑), HwaJe(和劑), KongJe(功劑) respectively. This study classified and analyzed major diseases and symptoms appeared in dermatosurgical prescription and composition of medicine, as well as in BangYakHapPyun(方藥合編). Results and conclusions : The results of examining dermatosurgical prescriptions in WonBang(元方) of SangJungHaTong(上中下統) in BangYakHapPyun(方藥合編) are as follows; 1. The proportion of dematosurgical prescriptions was SangTong(上統) $\frac{10}{126}$(7.9$\%$). JungTong(中統) $\frac{22}{181}$(12.1$\%$), and HaTong(下統) $\frac{16}{163}$(9.8$\%$), which means that JungTong(中統)(HwaJe 和劑) takes up relatively the largest portion. 2. As for SangTong(上統), upper level herbs used in medicine are Glycyrrhiza uralensis(甘草), Paeonia japonica(白芍藥), Angelica gigas(當歸). Astragalus membranaceus(황기). Ginseng(人蔘), Poria cocos(복령), Atractylodis macrocephalae rhizoma(白朮). Cinnamon(肉桂), Rehmaniniae radix preparat(熱地黃). And these herbs are the components of Sipjundaebo-tang(十全大補湯), one of the most well-known medicine for weak energy and blood(補氣血). 3. As for JungTong(中統), in addition to medicine for weak energy and blood. Ledebouriella seseloides(防風) that removes ill elements on skin surface and Pung(風) called "wind". Limonium tetragonum(桔梗) that eliminates discharges and sputum, Angelica dahurica(白芷) that removes discharge and suppress tumor are applied. Other herbs are Ostericum koreanum(羌活). Skullcap(황령),Schizonepeta tenuifolia(荊芥), Aurantii fructus(地殼), Cimicifuga heracleifolia(升麻), Bupleurum falcatum(柴胡), Lonicerae flos(金銀花). These herbs are more effective for wind-calming treatment. cooling down fever, clearing skin irritation, detoxication. removal of tumor and discharge than replenishing energy and blood. 4. As for HaTong(下統), Angelica gigas(當歸) and Ledebouriella seseloides(防風), the two major herbs for SangTong(上統) and JungTong(中統), are mostly used. In addition, Skullcap(黃芩), Gardenia jasminoides(梔子), Eisenia bicyclis(大黃) are other major components and their key efficacy is to lower fever and KongHa(功下). 5. Herbs applied for SangTong(上統), JungTong(中統), and HaTong(下統) in large quantity are Glycyrrhiza uralensis(甘草) that harmoniously combine different herbal elements and Poria cocos(복령) that discharges humidity and watery elements out of body, removes humid and hot elements, and strengthen gastrointestinal system. Based on this, it is inferred that prescriptions for this study focus largely on treatment of humid and hot elements. In the composition of this prescription, Angelica gigas(當歸), Paeonia japonica(白芍藥), and Cnidium officinale(川芎) are taking up relatively large proportion, which are basic herbs for Samul-tang(四物湯). Therefore, it is incurred here that the concept of "replenishing blood" bears importance in dermatosurgical treatment. 6. As for herb medicines used for more than two types of prescriptions of SangTong(上統), JungTong(中統), and HaTong(下統), most of them are simultaneously used for SangTong(上統) and JungTong(中統), or for JungTong(中統), and HaTong(下統) except for Atractylodis macrocephalae rhizoma(白朮) and Gleditsia sinensis(조각자). This finding implies that prescription or treatment that are simultaneously applied are replenishing and harmonizing, or harmonizing and attacking while replenishing and attacking never go together.

• 복식문화연구
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• 제14권5호
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• pp.802-812
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• 2006

The purpose of this study is to investigate effects of natural incense on the preservation of paper- and textile-based remains and the kinds and applications of natural anti-insect incense by reviewing relevant literatures of the ancient times. There are few ancient literatures of incense published in Korea. The researcher deducted how incense was used in this nation through reviewing verses contained in ancient literatures and medical books. In contrast, the kinds and applications of anti-insect incense used in China, where incense culture prospered, were investigated here through reviewing technical books about incense published during the Song(宋) and Ming(明) periods, $\ll$Incense record(香譜)$\gg$, $\ll$Chen's Incense record(陳氏香譜)$\gg$ and $\ll$Incense record(香乘)$\gg$. There were several methods of keeping clothes better from insects. In relation, how to use anti-insect incense varied in accordance with main materials of clothes, paper, textile, leather and others. Cymbopogon dstans(芸香), Brassica rapu var and Incarvillea sinensis(角蒿) are anti-insect incense which were used for paper. Anti-insect incense for textiles is classified into single and mixed incenses depending on whether only one kind of incense was used or more than seven kinds of the substance. Acori rhizoma(菖蒲), Capsella bursa-pastoris(薺菜花), Lactuca sativa L., Erigeron canadensis(莽草), Stemona japonica(百部) and Moschus sifanicus(麝香) are single anti-insect incense which were used for textile. While, the latter was called. ‘Yi Xiang(衣香)’ is mixed anti-insect incense which was used for textile. Artemisia asiatica(艾葉) and Zanthoxylum schinifolium(花椒) are anti-insect incense which were used for leather. Angelica dahurica(芳香) and Bamboo are anti-insect incense which were used for others. There were three main methods of using incense to prevent insects, that is, diffusing incense's strong scent and ingredients, exposing to smokes from burnt incense and washing with incense-boiled water. Diffusing incenses had a strong scent and antibiotic ingredients, which were put between books or clothes or into a storage box without being processed. If necessary, however, they were processed into rough powders that were in turn used singly, or otherwise mixed for a stronger scent and better insect elimination. Exposing to smokes from burnt incense was done as follows. A clothes was put on 'Long(籠)' underneath which there was a boiling water. The clothes was humidified by the water and then exposed to smokes from burnt incense. 'Long(籠)' had been long used since it was manufactured in the QinHan(秦漢) period for the first time. A local literature, $\ll$Koryo TuGing(高麗圖經)$\gg$ shows that in the Koryo(高麗) period, BoShaLu(博山爐) were used as a means of exposing clothes to smokes to prevent moths, similarly to China. Washing clothes with incense-boiled water was more effective in removing lots of worms and germs from clothes, but leaving the scent and ingredients of the used incense and maintaining the effect of anti-insect.

• 한국식품영양과학회지
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• 제39권1호
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• pp.154-158
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• 2010

본 연구에서는 현미 발아 시 침지 및 발아 조건에 따른 GABA 함량의 변화를 분석하고 효과적으로 GABA 함량을 증가시키기 위한 처리조건을 살펴보았다. 발아 현미의 GABA 함량 증가를 위해 침지 전 열처리를 실시하였으며, 한약재로 널리 쓰이는 오미자와 용안육, 구릿대, 천마를 침지수와 발아수로 이용하였을 때의 GABA 함량의 변화를 확인하였다. 실험 결과 $45^{\circ}C$에서의 열처리군이 3977.3 nmol/g로 무처리군에 비해 약 1.4배 정도의 증가 효과를 나타내었다. 또한 한약재를 침지 및 발아수로 이용한 결과 용안육은 3% 추출물을 발아수로 이용하였을 경우가 가장 높은 증가율을 보여주었으며, 오미자는 5% 추출물을 발아수로 이용한 경우가 대조군보다 2.1배로 가장 높은 GABA 함량을 나타내었다. 천마 추출물의 경우는 5% 추출물을 침지수와 발아수로 이용했을 경우 약 2.5배의 증가율을 보여 실험한 한약재 중 가장 높은 효과를 보여주었다. 본 연구 결과는 점차 관심이 높아지고 있는 발아현미 연구에 있어서 기초자료가 될 것으로 예상되며, 쌀 소비촉진에 영향을 미칠 것으로 기대된다.

• 동의생리병리학회지
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• 제19권3호
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• pp.633-639
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• 2005

The composition of Hwoangbaec-tang has been traditionally used in Korea to treat cancer. Hwoangbaec-tang I is the water extracts prepared from Angelica dahurica, Fritillariae verticillata, Ailanthus altissima, Viscum coloratun, Scutellaria Radix, Ginseng Radix, Astragalus membranaceus, and Glycyrrhizae Radix. Hwoangbaec-tang II also is the water extracts prepared from Ginseng Radix, Astragalus membranaceus, and Glycyrrhizae Radix. The anti-leukemic effects of human promyelocytic leukaemia (HL-60 cells) by Hwoangbaec-tang I or II was accessed by propidium iodide staining flow cytometric analysis, and apoptosis-inducing activity was further confirmed by a nuclear morphological change, a ladder pattern of DNA fragmentation, and an activation of caspase-3 and 9. Hwoangbaec-tang I was found to induce the apoptosis of HL-60 cells via caspase-3 and 9 pathway. In the other side, Hwoangbaec-tang II was found to inhibit the growth of HL-60 cells by inducing these cells to differentiate toward granulocytes. These results indicate that the different anti-leukemic effects of Hwoangbaec-tang in HL-60 cells can be induce the apoptosis or differnetiation of HL-60 cells in Hwoangbaec-tang composition dependent manner.

• Journal of Applied Biological Chemistry
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• 제65권4호
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• pp.343-348
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• 2022

백지는 산형과(Umbelliferae)에 속하는 구릿대(Angelica dahurica)의 뿌리를 건조한 것으로, 다양한 약리작용을 하는 것으로 알려져 예로부터 한약재로 사용되어 왔다. 백지의 대표적인 활성 성분인 coumarin을 포함하여 다양한 성분이 알려져 있으며, 최근 백지의 다양한 생리활성이 보고되고 있다. 본 연구는 백지를 건강기능식품의 기능성 원료로 활용하고자, 백지의 지표성분 설정 및 이에 대한 UPLC 분석법을 확립하여 분석법 유효성 검증을 실시하였다. 백지의 지표성분으로는 oxypeucedanin hydrate, byakangelicol, oxypeucedanin, imperatoin, phellopterin, isoimperatorin의6종을 설정하였다. 분석법 유효성 검증은 특이성, 직선성, 검출한계, 정량한계, 정확성, 정밀성 등의 항목을 이용하여 확인하였다. 본 시험법에서 지표성분 6종은 서로 간섭없이 분리되어 특이성을 확인하였고, 결정계수의 값도 0.999 이상으로 나타나 높은 직선성을 나타내었다. 분석법 유효성 검증 결과, 지표 성분 6종의 RSD는 5% 이하로 나타났으며 90-110%의 범위의 회수율을 나타내 높은 정확성과 정밀성이 있는 것을 확인할 수있었다. 이러한 결과는 UPLC를 이용한 백지의 지표성분 분석법이, 새롭게 제시한 6종에 대해 적합한 동시분석 분석법임을 나타낸다. 따라서, 본 연구를 통하여 확립된 백지의 지표성분 분석법이 향후 백지(구릿대)의 건강기능식품 기능성 원료 개발을 위한 기초자료로 활용될 것으로 기대된다.