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http://dx.doi.org/10.3839/jabc.2022.044

Method validation of marker compounds from Angelicae Dahuricae Radix as functional food ingredients  

Bo-Ram Choi (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Dahye Yoon (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Hyeon Seon Na (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Geum-Soog Kim (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Kyung-Sook Han (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Sookyeong Lee (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA)
Dae Young Lee (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Publication Information
Journal of Applied Biological Chemistry / v.65, no.4, 2022 , pp. 343-348 More about this Journal
Abstract
This study was performed to establish an analytical method for the standardization of Angelicae Dahuricae Radix as a functional ingredient. We established six compounds including oxypeucedanin hydrate (1), byakangelcol (2), oxypeucedanin (3), imperatorin (4), phellopterin (5) and isoimperatorin (6) as marker compounds of Angelicae Dahuricae Radix. An analytical method using Ultra Performance Liquid Chromatography (UPLC) was established and validated for marker compounds of Angelicae Dahuricae Radix. The specificity was confirmed by the chromatogram from UPLC and the value of coefficient determination was also higher than 0.999, indicating high linearity. The relative standard deviation (RSD) and recovery of marker compounds were less than 5% and in the range of 90- 110%, respectively, which means that this method has high accuracy and precision. Therefore, this analytical method could be used as basic data for the development of functional ingredients for health functional food of Angelicae Dahuricae Radix.
Keywords
Angelicae Dahuricae Radix; Functional health food; Marker compounds; Method validation; Ultra Performance Liquid Chromatography;
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