Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Anti-leukemic Effects of Hwoangbaec-tang in Human Promyelocytic Leukaemia, HL-60 Cells

항백탕 추출물의 인간 백혈병 세포주 HL-60에서 항백혈병 효과

  • Kim, Young-Jun (Department of Microbiology and Immunology, Wonkwang University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Ju, Sung-Min (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Jang-Chun (Department of Prescription, College of Oriental Medicine, Sangji University) ;
  • Park, Yang-Koo (Department of Prescription, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Sang-Hyun (Department of Prescription, College of Oriental Medicine, Wonkwang University) ;
  • Chon, Young-Gyun (Department of Prescription, College of Oriental Medicine, Wonkwang University) ;
  • Hwang, Joo-Min (Department of Prescription, College of Oriental Medicine, Wonkwang University) ;
  • Lim, Dae-Hwan (Department of Prescription, College of Oriental Medicine, Wonkwang University) ;
  • Yun, Young-Gab (Department of Prescription, College of Oriental Medicine, Wonkwang University)
  • 김용준 (원광대학교 의과대학 미생물학 및 면역학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 주성민 (원광대학교 한의과대학 병리학교실) ;
  • 이장천 (상지대학교 한의과대학) ;
  • 박양구 (원광대학교 한의과대학 방제학교실) ;
  • 이상현 (원광대학교 한의과대학 방제학교실) ;
  • 전영균 (원광대학교 한의과대학 방제학교실) ;
  • 황주민 (원광대학교 한의과대학 방제학교실) ;
  • 임대환 (원광대학교 한의과대학 방제학교실) ;
  • 윤용갑 (원광대학교 한의과대학 방제학교실)
  • Published : 2005.06.25
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Abstract

The composition of Hwoangbaec-tang has been traditionally used in Korea to treat cancer. Hwoangbaec-tang I is the water extracts prepared from Angelica dahurica, Fritillariae verticillata, Ailanthus altissima, Viscum coloratun, Scutellaria Radix, Ginseng Radix, Astragalus membranaceus, and Glycyrrhizae Radix. Hwoangbaec-tang II also is the water extracts prepared from Ginseng Radix, Astragalus membranaceus, and Glycyrrhizae Radix. The anti-leukemic effects of human promyelocytic leukaemia (HL-60 cells) by Hwoangbaec-tang I or II was accessed by propidium iodide staining flow cytometric analysis, and apoptosis-inducing activity was further confirmed by a nuclear morphological change, a ladder pattern of DNA fragmentation, and an activation of caspase-3 and 9. Hwoangbaec-tang I was found to induce the apoptosis of HL-60 cells via caspase-3 and 9 pathway. In the other side, Hwoangbaec-tang II was found to inhibit the growth of HL-60 cells by inducing these cells to differentiate toward granulocytes. These results indicate that the different anti-leukemic effects of Hwoangbaec-tang in HL-60 cells can be induce the apoptosis or differnetiation of HL-60 cells in Hwoangbaec-tang composition dependent manner.

Keywords

References

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