• The Journal of Internal Korean Medicine
• /
• v.21 no.1
• /
• pp.156-161
• /
• 2000

The unripe fruit of Poncirus trifoliata Raf has been used for the treatment of allergic disease. Recently it was reported that the fruit inhibits passive cutaneous anaphylaxis and histamine release at mast cell. Type I immediate hypersensitivity of anaphylactic type is caused by released mediate chemical at mast cell. Histamine is also known as one of potent mediate chemical. Also release of mediate chemical is affected by specific stimulation of IgE combined with mast cell. Activation of mast cell is known to be stimulated by compound 48/80 and inhibited by increase of cAMP. In this experiment, the effect of water extract of Poncirus trifoliata Raf fruit (PT) on a histamine release, cAMP concentration and IgE production was measured. Compound 48/80 was administrated to the mouse peritoneal cell which was pretreated with PT. PT dosedependently inhibited histamine release at peritoneal mast cell and the serum level of histamine induced by compound 48/80. PT also instantly increased cAMP level of peritoneal mast cell right after it was added and the level gradually decreased. Production of IgE induced by antigens at mouse peritoneal cell was inhibited by PT. The IgE synthesis is induced by IL-4 and it is known that lipopolysaccharide(LPS) plus IL-4 cause an increase in IgE secretion by murine B cells. The effects of PT inhibited the production of IgE activated by LPS plus IL-4 at human U266B1 cells. These results indicate that PT has antiallergic activity by Inhibition of IgE production from B cells and histamine release by increase of cAMP.

• Parasites, Hosts and Diseases
• /
• v.37 no.3
• /
• pp.171-179
• /
• 1999

Non-biting midges are known to contain potent inhalant allergens. IgE antibody responses to the crude extract of Chironomus kiiensis adults, a dominant chironomid species in Korea, were examined. With the IgE-ELISA or passive cutaneous anaphylaxis reactions, increased levels of chironomid-specific IgE were detected in the skin test positived human sera, or immunized BALB/c mouse sera with the crude extract adsorbed to alum. IgE-immunoblot analysis showed mafor IgE-reacting protein band patterns, which reacted with more than 50% of the skin test positive human sera, at 110, 80, 46, 40, 37, 34 and 31 kDa. The reactive band patterns were larely similar between skin test positive humans and immune BALB/c mice. However, the bands of 55, 31, 27, 26, 24 and 23 kDa were found only in sensitized humans, but not in immunized mice.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.19 no.6
• /
• pp.1659-1665
• /
• 2005

Gamipaidok-san(GPDS) is the Hyungbangpaidok-san prescription fortified with the additional ingredients known to be effective for dermatitis. So it has been used for atopic dermatitis in the clinic work actually. In this study, we investigated the effects of GPDS on in vitro and in vivo anti-allergic effect on RBL-2H3 rat basophilic leukemia Cells and on IgE-induced passive cutaneous anaphylaxis (PCA) in mice. The in vitro anti-inflammatory activity of GPDS in RAW 264.7 cells was investigated. GPDS potently inhibited $\beta$-hexosaminidase release from RBL-2H3 and the IgE-mediated PCA reaction in mice. GPDS inhibited LPS-induced NO and PGE2 production in a dose-dependent manner Furthermore, It also inhibited inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression in RAW 264.7 cells, and the activation of the transcription factor, NF-kB, in nuclear fraction. The antiallergic action of GPDS may originate from anti-inflammatory activities, and can improve the inflammation caused by allergies.

• The Korean Journal of Food And Nutrition
• /
• v.11 no.2
• /
• pp.228-236
• /
• 1998

Egg is an important foods containing many good proteins. But it is well known that egg protein has a lot of allergenicity. The purpose of this study is to develop the methods to reduce the allergenicity of egg. I tried various experimental methods ; For example, heat treatment, irradiation with ultraviolet and microwaves, treatment with polyphosphate, enzyme hydrolysis and PCA inhibition test using guinea pigs and degrees of hydrolysis. The results obtained were as follows ; 1. Heat treatment reduced allergenicity of egg protein. The longer the heat time, the better the effect. 2. Irradiating with ultraviolet and microwave increased both the degree of protein hydrolysis and PCA inhibition reduced the allergenicity. Ultraviolet was more effective than microwaves on egg protein. Fertilized eggs did not reduce allergenicity. 3. Enzyme treatment increased the degree of hydrolysis and PCA inhibition, and reduced allergenicity considerably. Alcalase was more effective than neutrase. 4. Adding polyphosphate did not induced protein hydrolysis, but increased PCA inhibition and reduced allergenicity. 5. The picture of various treatments of egg gel by SEM showed a light surface which indicated that protein was desolved. Neutrase was lighter than alcalase, and the longer the heating time, the lighter the surface became. 6. Measurements of the hardness of egg gel by Instron showed that the longer the reaction time with enzyme, the softer it became.

• Korean Journal of Acupuncture
• /
• v.20 no.3
• /
• pp.81-90
• /
• 2003

Objectives : This experimental research has been done to study the effects of herbal-acupuncture with Juglandis Semen on the anti-allergic reaction. Methods : ICR mice were injected intraperioneally with compound 48/80 to induce the immediately type hypersensitivity (anaphylaxis). ICR mice were sensitized by PC and SRBC to induce the delayed type hypersensitivity. Sprage-Dawley rats were injected subcutaneously with egg albumin to measure the WBC. ICR mice and Sprage-Dawley rats were injected with Juglandis Semen herbal-acupuncture for the anti-allergic reaction test. Results : 1. In the restraint effect of systemic anaphylactic reaction, the mortality of mice decreased 20% each before 10 minutes and after 5 minutes. 2. In the contact dermatitis induced by PC, both Treat Ⅰand Treat Ⅱ group showed significant(p<0.01, p<0.05) decrease in comparison with the Control group, and also Treat Ⅲ group showed significance(p<0.005). 3. In the delayed type hypersensitivity in mice challenged by SRBC, Treat Ⅰ group showed significant(p<0.05) decrease in comparison with the Control group, and there was significance(p<0.05, p<0.05) in Treat Ⅲ and Treat Ⅳ group. 4. The change of the WBC in allergic rats induced by egg albumin showed significant(p<0.01, p<0.05, p<0.05) decrease in Treat Ⅰ, Treat Ⅱ and Treat Ⅲ group. Conclusions : The restraint effects of Juglandis Semen herbal-acupuncture on the anti-allergic reaction showed significance statistically. And also allergic group treated with $BL_{17}$ acupuncture (Treat Ⅲ group) showed same results.

• The Journal of Korean Medicine
• /
• v.18 no.1
• /
• pp.316-325
• /
• 1997

The dried unripe fruit of Poncirus trifoliata L. is widely used to treat urticaria, itch and indigestion in folk medicine. And recently it was reported the component of the fruit was found to exhibit an inhibitory effect on histamine release from mast cells. So to evaluate the effect of aqueous extract of Poncirus trifoliata L.(PTFE) on compound 48/80-induced histamine release, the study was carried out in rat peritoneal mast cell. PTFE $(10^{-3}{\sim}1mg/ml)$ inhibits the histamine release induced by compound 48/80 $(5{\mu}g/ml)$ in rat peritoneal mast cells. To clarify the mechanism of these inhibition, we investigated the effect of PTFE on cAMP and intracellular calcium content. The increase in cAMP content, when PTFE was added, was transient. At concentration of 1mg/ml, the cAMP content of mast cells was significantly increased at a rate of 53 times of basal cells at 10sec. PTFE inhibits histamine release by augmenting the cAMP content in mast cells. Moreover, PTFE inhibits intracellular calcium release induced by compound 48/80. This result suggests that PTFE may be useful for the prevention and treatment of allergy-related disease.

• Journal of Dental Anesthesia and Pain Medicine
• /
• v.17 no.3
• /
• pp.163-181
• /
• 2017

In the field of orofacial surgery, a red blood cell transfusion (RBCT) is occasionally required during double jaw and oral cancer surgery. However, the question remains whether the effect of RBCT during the perioperative period is beneficial or harmful. The answer to this question remains challenging. In the field of orofacial surgery, transfusion is performed for the purpose of oxygen transfer to hypoxic tissues and plasma volume expansion when there is bleeding. However, there are various risks, such as infectious complications (viral and bacterial), transfusion-related acute lung injury, ABO and non-ABO associated hemolytic transfusion reactions, febrile non-hemolytic transfusion reactions, transfusion associated graft-versus-host disease, transfusion associated circulatory overload, and hypersensitivity transfusion reaction including anaphylaxis and transfusion-related immune-modulation. Many studies and guidelines have suggested RBCT is considered when hemoglobin levels recorded are 7 g/dL for general patients and 8-9 g/dL for patients with cardiovascular disease or hemodynamically unstable patients. However, RBCT is occasionally an essential treatment during surgeries and it is often required in emergency cases. We need to comprehensively consider postoperative bleeding, different clinical situations, the level of intra- and postoperative patient monitoring, and various problems that may arise from a transfusion, in the perspective of patient safety. Since orofacial surgery has an especially high risk of bleeding due to the complex structures involved and the extensive vascular distribution, measures to prevent bleeding should be taken and the conditions for a transfusion should be optimized and appropriate in order to promote patient safety.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.6 no.1
• /
• pp.15-29
• /
• 1993

Yenhwagamchotang has been widely used in treatment of inflammatory disease which is based on Oriental Medical literatures. These studies were attempted experimental effects of Yenhwagamchotang(sample A), Yenhwagamchotang plus Forsythia koreana NAKAI.(sample B), and Yenhwa-gamchotang plus Taraxacum mongolicum HAND- MAZZ(sample C),on the Anti-allergic reaction, the antipyretic action,the anti-inflammatory and the analgesic action,in rats. THe results of the studies were as follow: 1. Vascular permeability responses to intradermal serotonin in rats were showed significant effect at all sample groups. 2. The homologous passive cutaneous anaphylaxis in rats provoked by the IgE-like antibody aganist egg white albumin showed the decreasing effect. 3. The delayed type hypersensitivity responses to picryl chloride in mice were showed significant effect at all sample groups. 4. The delayed type hypersensitivity response to sheep red blood cell in mice were showed significant effect at all sample groups. 5. In anti-pyretic effect by yeast method were showed significant effect at all sample groups. 6. The anti-inflammatory effect by carrageenin method were showed significant effect at all sample groups. 7. The analgesic action by acetic acid method in mice were showed significant effect at all sample groups. According to the above result, Yenhwagamchotang(sample A), Yenhwagamchotang plus Forsythia koreana NAKAI(sample B), AND yenhwagamchotang plus Taraxacum mongolicum HNAN- MAZZ.(sample C ) were concluded to have the anti-allergic reaction, the antipyretic action, the anti-inflammatory, the analgesic action.

• The Journal of Korean Medicine
• /
• v.19 no.2
• /
• pp.439-449
• /
• 1998

Anal Therapy is another way of taking medicine. It is a traditional pathway but not available in common situation. Nevertheless, It has many benifect and usefulness, it has not treated so much. Through Anal Therapy, the valid compound of Herb med can be reach to the desination in theory of the organism and loca1 medical action. The former is called Jung-Chei Theory(整體論), which is the one of the most important basements in building traditional Korean medicine. As there are many kinds of Anal therapy, this study use reservation type. Sosihotang(SSHT) is one of the well-known korean medicines for a long time. It is used for the treatment of such dieases as infectious diseases, hepatic diseases and gastroenteritis and so on. In this study, the author investigated the effect of an aqueous extract of SSHT by Anal therapy(Reservative Enema) in anaphylactic shock. The following results were obtained 1. SSHT inhibited anaphylactic shock 100% with a dose of 1.0 g/kg 1 hr before intraperitoneal injection of compound 48/80. SSHT significantly reduced serum histamine contents induced by compound 48/80. 2. SSHT (0.1 g/kg) also inhibited to 30.9% (P<0.05)) local cutaneous anaphylactic reaction activated by anti-dinitrophenyl (DNP) IgE. 3. The validity rate of reservative enema is as much as oral pathway. 4. In addition, SSHT dose-dependently inhibited the histamine release from the peritoneal mast cells by compound 48/80 or anti-DNP IgE. These results provide evidence that Anal Therapy(Reservative enema) of SSHT may be beneficial in the treatment of systemic and local anaphylactic reaction. Moreover, I wish another much sincere study of Anal Therapy (Reservative enema) would be obtained.

• Journal of Pharmaceutical Investigation
• /
• v.30 no.3
• /
• pp.159-166
• /
• 2000

Kunitz-type soybean trypsin inhibitor (SBTI) and chondroitin sulfate (A, and C type) were conjugated using sodium periodate method. And the physicochemical, pharmacokinetic properties and immunogenecity of the conjugates (Chon-A-SBTI or Chon-C-SBTI) were characterized. We expected the conjugation using chondroitin sulfate to reduce the immunogenecity and to improve the pharmacological effect. As the results, the mean molecular weight of the conjugate highly increased. After I.V. injection of the radiolabeled conjugates or native SBTI into mice, it was found that native SBTI showed rapid elimination from plasma, whereas Chon-A-SBTI and Chon-C-SBTI were slowly eliminated. Organ distribution of the two agents at 30 min after I.V. injection was different : Chon-A-SBTI or Chon-C-SBTI accumulated to a large extent in the liver (13% in Chon-A-SBTI and 16% in Chon-C-SBTI), whereas native SBTI was taken up more rapidly by the kidney (107% dose/g of tissue) and excreated into the urine (26%). In addition we evaluated the therapeutic value of the conjugates by using the sublethal septic shock model caused by pseudomonal elastase and tested the immunogenecity by passive cutaneous anaphylaxis shock (PCA). The conjugates were more effective than native SBTI against pseudomonal elastase induced septic shock in guinea pig. In case of the conjugates, the pharmacological and therapeutic effect lasted over 3 hours long. In immunogenecity test, both of the conjugates showed the reduction of their immunogenecity, especially Chon-A-SBTI looked most effective.