• 제목/요약/키워드: Analytical precision

검색결과 818건 처리시간 0.02초

Theoretical analysis of Y-shape bridge and application

  • Lu, Peng-Zhen;Zhang, Jun-Ping;Zhao, Ren-Da;Huang, Hai-Yun
    • Structural Engineering and Mechanics
    • /
    • 제31권2호
    • /
    • pp.137-152
    • /
    • 2009
  • Mechanic behavior of Y-shape thin-walled box girder bridge structure is complex, so one can not exactly hold the mechanical behavior of the Y-shape thin-walled box girder bridge structure through general calculation theory and analytical method. To hold the mechanical behavior better, based on elementary beam theory, by increasing the degree of freedom analytical method, taking account of restrained torsiondistortion angledistortion warp and shearing lag effect at the same time, authors obtain a thin-walled box beam analytical element of 10 degrees of freedom of every node, derive stiffness matrix of the element, and code a finite element procedure. In addition, authors combine the obtained procedure with spatial grillage analytical method, meanwhile, they build a new analytical method that is the spatial thin-walled box girder element grillage analysis method. In order to validate the precision of the obtained analysis method, authors analyze a type Y-shape thin-walled box girder bridge structure according to the elementary beam theory analytical method, the shell theory analytical method and the spatial thin-walled box girder element grillage analysis method respectively. At last, authors test a type Y-shape thin-walled box girder bridge structure. Comparisons of the results of theory analysis with the experimental text show that the spatial thin-walled box girder element grillage analysis method is simple and exact. The research results are helpful for the knowledge of the mechanics property of these Y-shape thin-walled box girder bridge structures.

Simultaneous Determination of Five Porphyrins in Human Urine and Plasma Using High Performance Liquid Chromatography-Tandem Mass Spectrometry

  • Hur, Yeoun;Tae, Sookil;Koh, Yun-Joo;Hong, Sung-Hyun;Yoon, Young Ho;Jang, Haejong;Kim, Sooji;Kim, Kyeong Ho;Kang, Seung Woo;Lee, Youngshin;Han, Sang Beom
    • Mass Spectrometry Letters
    • /
    • 제5권2호
    • /
    • pp.42-48
    • /
    • 2014
  • A specific and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry method (LC-ESI-MS/MS) was developed and validated for the simultaneous quantification of porphyrins (coproporphyrin, pentacarboxylporphyrin, hexacarboxylporphyrin, heptacarboxylporphyrin, and uroporphyrin) in human plasma and urine. Acidified plasma samples and urine samples were prepared by using liquid-liquid extraction using ethyl acetate and protein precipitation with acetonitrile, respectively. The separation was achieved onto a Synergi Fusion RP column ($150mm{\times}2.0mm$, $4{\mu}m$) with a gradient elution of mobile phase A (0.1% formic acid in 2 mmol/L ammonium acetate, v/v) and mobile phase B (20% methanol in acetonitrile, v/v) at a flow rate of $450{\mu}L$/min. Porphyrins and the internal standard (IS), coproporphyrin I-$^{15}N_4$, were detected by a tandem mass spectrometer equipped with an electrospray ion source operating in positive ion mode. Multiple reaction monitoring (MRM) transitions of the protonated precursor ions and the related product ions were optimized to increase selectivity and sensitivity. The proposed method was validated by assessing selectivity, linearity, limit of quantification (LOQ), precision, accuracy, recovery, and stability. The calibration curves were obtained in the range of 0.1-100 nmol/L and the LOQs were estimated as 0.1 nmol/L for all porphyrins. Results obtained from the validation study of porphyrins showed good accuracy, precision, recovery, and stability. Finally, the proposed method was successfully applied to clinical studies on the autism spectrum disorder (ASD) diagnosis of 203 Korean children.

페노피브레이트 유연물질 표준품 대체시험법 개발 (Development of an Alternative Analytical Method without Related Substance Reference Standards for Fenofibrate in Korean Pharmacopoeia)

  • 김정현;김민영;권은경;이광문;최돈웅
    • 약학회지
    • /
    • 제59권3호
    • /
    • pp.98-106
    • /
    • 2015
  • Analytical method for related substances can be categorized into two methods depending on the necessity of reference standard (RS). The analytical method of related substances with RS is fast and accurate, but it's very expensive and technically difficult to synthesize RS due to their complicated structure. Another method is using relative retention time (RRT) and relative response factor (RRF) which are already validated with RS. Validation of this method is not easy and time consuming, but once it has been developed, it can save cost and time. In this study, we developed the analytical method for related substances of fenofibrate using RRT and RRF. We validated the method by evaluating specificity, linearity, accuracy and precision according to the "Manual for Guideline Application for Validation of Analytical Procedures" of MFDS. Also, we calculated RRT and RRF between fenofibrate and fenofibrate related substances. The results of this study showed high specificity for fenofibrate and fenofibrate related substances. Correlation coefficient(r) of all substances were more than 0.99, and the recovery of fenofibrate, fenofibrate related substance I, II and III were 99.44%, 100.84%, 99.14% and 101.58%, respectively. Precision of fenofibrate and its related substances were ranged between RSD 0.29% and 0.93%. Quantification limits of fenofibrate, fenofibrate related substance I, II and III were determined to be $0.03{\mu}g/ml$, $0.05{\mu}g/ml$, $0.04{\mu}g/ml$ and $0.02{\mu}g/ml$, respectively by confirming signal to noise ratio of each chromatogram. The RRT for fenofibrate related substance I, II and III were determined to be 0.35, 0.41 and 1.34, respectively. Also, the RRF for fenofibrate related substance I, II and III were determined to be 1.28, 0.98 and 0.79, respectively. The developed method was applied to determine contents for fenofibrate related substances in commercial fenofibrate (active pharmaceutical ingredient). As a result, developed analytical methods of related substances will be used for revising the monograph of fenofibrate in Korean Pharmacopoeia revision and contribute quality control of drugs by improving cost and time consuming problem of RS.

소형챔버를 이용한 건축자재 오염물질 방출시험방법 평가 (Evaluation of sampling and analytical method for emission experiment of pollutants in building materials using small chamber)

  • 이석조;장성기;김미현;이홍석;임준호;장미;서수연
    • 분석과학
    • /
    • 제18권4호
    • /
    • pp.344-354
    • /
    • 2005
  • 최근 들어 실내공기질 악화의 주 오염원인 건축자재에 대한 관심이 고조되면서 건축자재 오염물질 방출시험 자료 구축이 중요하게 됨에 따라, 신뢰성 있는 자료 확보를 위해 건축자재 방출시험 정도 관리에 대한 필요성이 대두되었다. 이에 본 연구는 건축자재 방출시험 성능에 대한 정도관리를 위해 총 휘발성 유기화합물과 포름알데히드에 대하여 기기분석 재현성, 회수율, 검출한계, 중복 및 반복채취 재현성, 파과용량 평가 등을 실시하였다. 기기분석 및 시료채취에 대한 재현성은 20~30% 이내의 양호한 결과를 보였으며, 회수율은 80% 이상으로 나타났으며, 파과 역시 일어나지 않아 소형챔버법에 의한 성능은 전반적으로 만족할 만한 수준을 보였다. 따라서, 소형챔버를 이용한 건축자재 오염물질 방출시험방법은 신뢰성있는 자료를 제공해 줄 수 있을 것으로 판단된다.

사진기준점측량을 이용한 저등급 기준점의 좌표결정 (Coordinates Determination of tow Level Control Point by Aerial Triangulation)

  • 최재화;이석배;최윤수
    • 한국측량학회지
    • /
    • 제6권2호
    • /
    • pp.34-41
    • /
    • 1988
  • 오늘날 항공사진측량은 사용기재와 자료처리방법의 발달과 더불어 위치결정의 정확도가 날로 향상되고 있으며, 지상기준점측량을 하지 않고서 GPS를 이용한 사진기준점측량 방안이 모색되는 단계에 이르렀다. 해석도화기의 등장은 정확도와 효율성면에서 상당한 발전을 가능케 하였고 On-Line System의 구성을 통해서 사진 기준점측량의 작업환경을 크게 개선시켰다. 본 연구에서는 해석도화기를 이용한 사진기준점측량을 실시하여 저등급 측지기준점의 좌표결정에 있어서 정확도의 향상을 그 목표로 하고 있다. 연구결과 정확도 향상의 정도는 기존의 정밀도화기를 사용한 A.T.의 경우와 비교할 때에 평면에서 약 7%, 표고에서 약 40%의 정확도 향상을 가져왔다.

  • PDF

Semi-analytical vibration analysis of functionally graded size-dependent nanobeams with various boundary conditions

  • Ebrahimi, Farzad;Salari, Erfan
    • Smart Structures and Systems
    • /
    • 제19권3호
    • /
    • pp.243-257
    • /
    • 2017
  • In this paper, free vibration of functionally graded (FG) size-dependent nanobeams is studied within the framework of nonlocal Timoshenko beam model. It is assumed that material properties of the FG nanobeam, vary continuously through the thickness according to a power-law form. The small scale effect is taken into consideration based on nonlocal elasticity theory of Eringen. The non-classical governing differential equations of motion are derived through Hamilton's principle and they are solved utilizing both Navier-based analytical method and an efficient and semi-analytical technique called differential transformation method (DTM). Various types of boundary conditions such as simply-supported, clamped-clamped, clamped-simply and clamped-free are assumed for edge supports. The good agreement between the presented DTM and analytical results of this article and those available in the literature validated the presented approach. It is demonstrated that the DTM has high precision and computational efficiency in the vibration analysis of FG nanobeams. The obtained results show the significance of the material graduation, nonlocal effect, slenderness ratio and boundary conditions on the vibration characteristics of FG nanobeams.

1,4-Dioxane의 분석방법 연구 (A Study on the Research of Analytical Method for 1,4-Dioxane in Water)

  • 박선구;김태호;임연택;명노일
    • 한국물환경학회지
    • /
    • 제21권6호
    • /
    • pp.549-563
    • /
    • 2005
  • The study was carried out to develop and standardize the analytical method of 1,4-dioxane in water. The National Institute of Environmental Research in Korea has monitored for 1,4-dioxane in water since 1998 and it has been detected in environmental water occasionally. But the environmental pollution and analytical method in water is not well known over the world. The trace analytical method at low-${\mu}/L$ level is prerequisite in order to evaluate the residue of 1,4-dioxane in water. Evaluation of the method was demonstrated by comparing and analyzing with liquid-liquid extraction, solid-phase extraction and purge & trap technique. As a result of experiment by standard solutions and raw samples, the precision and accuracy for three methods were obtained within error rate of about 10%. Therefore, three methods were standardized as official monitoring method in Korea. Also, a convenient and simple liquid-liquid extraction method for the analysis of 1,4-dioxane in water was developed by combined with gas chromatography/mass spectrometry and applied to the water samples in Korea

Development of Sensitive Analytical Method of Rhodanthpyrone A by a LC-MS/MS and its Application to Bioavailability Study in Rats

  • Kang, Bitna;Yoon, Jeong A;Song, Im-Sook;Han, Young Taek;Choi, Min-Koo
    • Mass Spectrometry Letters
    • /
    • 제10권3호
    • /
    • pp.88-92
    • /
    • 2019
  • A sensitive analytical method of rhodanthpyrone A in rat plasma was developed using a liquid chromatography-tandem mass spectrometry (LC-MS/MS). Rhodanthpyrone A and rhodanthpyrone B (internal standard) in rat plasma were extracted by a liquid-liquid extraction method with ethyl acetate. This extraction method gave results in high and reproducible extraction recovery in the range of 73.75-79.90% with no interfering peaks around the peak elution time of rhodanthpyrone A and B. The standard calibration curves for rhodanthpyrone A ranged from 0.5 to 2000 ng/mL were linear with $r^2$ > 0.994 and the inter- and intra-day accuracy and precision and the stability were within acceptance criteria. Using this validated analytical method, pharmacokinetics of rhodanthpyrone A following intravenous and oral administration of rhodanthpyrone A at doses of 2 mg/kg and 30 mg/kg, respectively, were investigated. Rhodanthpyrone A in rat plasma showed multi-exponential elimination pattern with high clearance and volume of distribution values. The absolute oral bioavailability of this compound was calculated as 3.7%. Collectively, the newly developed sensitive LC-MS/MS analytical method of rhodanthpyrone A could be successfully applied to investigate the pharmacokinetic properties of this compound and would be useful for the further studies on the efficacy, toxicity, and biopharmaceutics of rhodanthpyrone A.

Determination of N-nitrosodimethylamine in zidovudine using high performance liquid chromatography-tandem mass spectrometry

  • Yujin Lim;Aelim Kim;Yong-Moon Lee;Hwangeui Cho
    • 분석과학
    • /
    • 제36권6호
    • /
    • pp.281-290
    • /
    • 2023
  • Zidovudine is an antiretroviral agent prescribed for the prevention and treatment of human immunodeficiency virus/acquired immune deficiency syndrome (HIV/AIDS). It is typically recommended to be used in combination with other antiretroviral drugs. Zidovudine has the potential to generate N-nitrosodimethylamine (NDMA) in the presence of dimethylamine and nitrite salt under acidic reaction conditions during the drug manufacturing process. NDMA is a potent human carcinogen that may be detected in drug substances or drug products. An analytical method was developed to determine NDMA in pharmaceuticals including zidovudine using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The analysis involved reversed-phase chromatography on a Kinetex F5 column with a mobile phase comprising water-acetonitrile mixtures. The detection of positively charged ions was conducted using atmospheric pressure chemical ionization (APCI). The calibration curve demonstrated excellent linearity (r = 0.9997) across the range of 1-50 ng/mL with a highly sensitive limit of detection (LOD) at 0.3 ng/mL. The developed method underwent thorough validation for specificity, linearity, accuracy, precision, robustness, and system suitability. This sensitive and specific analytical method was applied for detecting NDMA in zidovudine drug substance and its formulation currently available in the market, indicating its suitability for drug quality management purposes.

Quantitative analysis and validation of naproxen tablets by using transmission raman spectroscopy

  • Jaejin Kim;Janghee Han;Young-Chul Lee;Young-Ah Woo
    • 분석과학
    • /
    • 제37권2호
    • /
    • pp.114-122
    • /
    • 2024
  • A transmission Raman spectroscopy-based quantitative model, which can analyze the content of a drug product containing naproxen sodium as its active pharmaceutical ingredient (API), was developed. Compared with the existing analytical method, i.e., high-performance liquid chromatography (HPLC), Raman spectroscopy exhibits high test efficiency owing to its shorter sample pre-treatment and measurement time. Raman spectroscopy is environmentally friendly since samples can be tested rapidly via a nondestructive method without sample preparation using solvent. Through this analysis method, rapid on-site analysis was possible and it could prevent the production of defective tablets with potency problems. The developed method was applied to the assays of the naproxen sodium of coated tablets that were manufactured in commercial scale and the content of naproxen sodium was accurately predicted by Raman spectroscopy and compared with the reference analytical method such as HPLC. The method validation of the new approach was also performed. Further, the specificity, linearity, accuracy, precision, and robustness tests were conducted, and all the results were within the criteria. The standard error of cross-validation and standard error of prediction values were determined as 0.949 % and 0.724 %, respectively.