• Title/Summary/Keyword: Analysis of potential profiles

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Effects of fermentation on protein profile of coffee by-products and its relationship with internal protein structure measured by vibrational spectroscopy

  • Samadi;Xin Feng;Luciana Prates;Siti Wajizah;Zulfahrizal;Agus Arip Munawar;Peiqiang Yu
    • Animal Bioscience
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    • v.36 no.8
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    • pp.1190-1198
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    • 2023
  • Objective: To our knowledge, there are few studies on the correlation between internal structure of fermented products and nutrient delivery from by-products from coffee processing in the ruminant system. The objective of this project was to use advanced mid-infrared vibrational spectroscopic technique (ATR-FT/IR) to reveal interactive correlation between protein internal structure and ruminant-relevant protein and energy metabolic profiles of by-products from coffee processing affected by added-microorganism fermentation duration. Methods: The by-products from coffee processing were fermented using commercial fermentation product, called Saus Burger Pakan, consisting of various microorganisms: cellulolytic, lactic acid, amylolytic, proteolytic, and xylanolytic microbes, for 0, 7, 14, 21, and 28 days. Protein chemical profiles, Cornell Net Carbohydrate and Protein System crude protein and CHO subfractions, and ruminal degradation and intestinal digestion of protein were evaluated. The attenuated total reflectance-Ft/IR (ATR-FTIR) spectroscopy was used to study protein structural features of spectra that were affected by added microorganism fermentation duration. The molecular spectral analyses were carried using OMNIC software. Molecular spectral analysis parameters in fermented and non-fermented by-products from coffee processing included: Amide I area (AIA), Amide II (AIIA) area, Amide I heigh (AIH), Amide II height (AIIH), α-helix height (αH), β-sheet height (βH), AIA to AIIA ratio, AIH to AIIH ratio, and αH to βH ratio. The relationship between protein structure spectral profiles of by-products from coffee processing and protein related metabolic features in ruminant were also investigated. Results: Fermentation decreased rumen degradable protein and increased rumen undegradable protein of by-products from coffee processing (p<0.05), indicating more protein entering from rumen to the small intestine for animal use. The fermentation duration significantly impacted (p<0.05) protein structure spectral features. Fermentation tended to increase (p<0.10) AIA and AIH as well as β-sheet height which all are significantly related to the protein level. Conclusion: Protein structure spectral profiles of by-product form coffee processing could be utilized as potential evaluators to estimate protein related chemical profile and protein metabolic characteristics in ruminant system.

Preservice Teachers' Belief Change Represented as Constructivist Profile Change (구성주의 프로파일로 표현된 예비교사들의 신념변화)

  • Kwak, Young-Sun
    • Journal of the Korean earth science society
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    • v.23 no.3
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    • pp.242-258
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    • 2002
  • This study investigated preservice teachers' understandings of the ontology and epistemology underlying constructivist notions of teaming. Throughout this study, the epistemological, ontological, and pedagogical characteristics for each teacher's developing views of learning were identified through four in-depth interviews. Data from interviews were used to create three profiles containing ontological beliefs, epistemological commitments and pedagogical beliefs. This study has demonstrated that the notion of a constructivist profile change has significant potential for informing the analysis and description of preservice teachers' beliefs changes. Major findings include: constructivist profile changes overtime, diversification of profile components over time, features of the teachers' pedagogical belief profile changes, and teachers' unawareness of their profiles. However, changes in ontological beliefs and epistemological commitments were not easy, nor were they easily internalized for these teachers. The implications of this research are that preservice teachers should be aware of coexisting different categories of their learning-to-teach profiles, and that teacher educators should provide these preservice teachers with instruction designed to change preservice teachers' profiles towards increasing constructivist views of teaching and learning and restricting other undesirable categories.

The Association Between Apolipoprotein E Genotype and Lipid Profiles in Healthy Woman Workers (건강한 여성 근로자에서 Apolipoprotein E와 혈중 지질 농도와의 관련성)

  • Moon, Gi-Eun;Sung, Sook-Hee;Chang, Youn-Koun;Park, Il-Keun;Paek, Yun-Mi;Kim, Soo-Geun;Choi, Tae-In;Jin, Young-Woo
    • Journal of Preventive Medicine and Public Health
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    • v.43 no.3
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    • pp.213-221
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    • 2010
  • Objectives: Plasma lipid profiles and Apolipoprotein E (ApoE) are established risk factors for cardiovascular disease (CVD). The knowledge of lipid profile may estimate the potential victims of cardiovascular disease before its initiation and progression and offers the opportunity for primary prevention. The most common ApoE polymorphism has been found to influence plasma lipid concentrations and its correlation with CVD has been extensively investigated in the last decade. Methods: The ApoE polymorphism and its influence on plasma lipid were investigated in healthy woman workers. The information on confounding factors was obtained through a self-administered questionnaire and ApoE polymorphism was investigated using PCR. Results: The relative frequencies of alleles E2, E3 and E4 for the study population (n = 305) were 0.127, 0.750 and 0.121, respectively. ApoE polymorphism was associated with variations in plasma HDL-cholesterol lipid profile. In order to estimate the independent effects of alleles E2 and E4, as compared with E3, on lipid profile, multiple regression was performed after adjustment for confounding variables such as age, BMI, blood pressure, education status, insulin, fasting glucose, HOMA-IR, menopause. ApoE2 had a negative association with HDL cholesterol and ApoE4 had a positive association with LDL cholesterol. Conclusions: This study identified that the ApoE and CVD risk factors contribute to the lipid profiles, similar to other studies. The analysis including dietary intake and other gene in further studies may help to identify clear effects on lipid profiles as risk factor for CVD.

Constipation anti-aging effects by dairy-based lactic acid bacteria

  • Mohamad Hafis Jaafar;Pei Xu;Uma-Mageswary Mageswaran;Shandra-Devi Balasubramaniam;Maheswaran Solayappan;Jia-Jie Woon;Cindy Shuan-Ju Teh;Svetoslav Dimitrov Todorov;Yong-Ha Park;Guoxia Liu;Min-Tze Liong
    • Journal of Animal Science and Technology
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    • v.66 no.1
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    • pp.178-203
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    • 2024
  • Constipation, which refers to difficulties in defecation and infrequent bowel movement in emptying the gastrointestinal system that ultimately produces hardened fecal matters, is a health concern in livestock and aging animals. The present study aimed to evaluate the potential effects of dairy-isolated lactic acid bacteria (LAB) strains to alleviate constipation as an alternative therapeutic intervention for constipation treatment in the aging model. Rats were aged via daily subcutaneous injection of D-galactose (600 mg/body weight [kg]), prior to induction of constipation via oral administration of loperamide hydrochloride (5 mg/body weight [kg]). LAB strains (L. fermentum USM 4189 or L. plantarum USM 4187) were administered daily via oral gavage (1 × 10 Log CFU/day) while the control group received sterile saline. Aged rats as shown with shorter telomere lengths exhibited increased fecal bulk and soften fecal upon administration of LAB strains amid constipation as observed using the Bristol Stool Chart, accompanied by a higher fecal moisture content as compared to the control (p < 0.05). Fecal water-soluble metabolite profiles showed a reduced concentration of threonine upon administration of LAB strains compared to the control (p < 0.05). Histopathological analysis also showed that the administration of LAB strains contributed to a higher colonic goblet cell count as compared to the control (p < 0.05). The present study illustrates the potential of dairy-sourced LAB strains as probiotics to ameliorate the adverse effect of constipation amid aging, and as a potential dietary intervention strategy for dairy foods including yogurt and cheese.

An Estimation of River bed Profile of the Stream System based on the Potential Energy Concept (位置에너지 槪念에 依한 水系의 河川縱斷 推定)

  • Ahn, Sang-Jin;Kang, Kwan-Won;Kim, Chang-Su
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.24 no.2
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    • pp.76-88
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    • 1982
  • The stream morphological characteristics of a basin have important influence upon the analysis of runoff. In this study, the laws of stream morphology-the law of average stream fall and the law of least rate of potential energy expenditure-which were derived based on the analogy of entropy in thermodynamics are introduced and their validity is analysised with the data taken from the topographic maps covering the whole Geum River system. The first law is the Law of Average Stream Fall which states that under the dynamic equilibrium condition the ratio of average fall between any two different order stream in the same river basin in unity. The second law is the law of least rate of energy expenditure which states that all natural streams are intended to choose their own course of flow such that the rate of potential energy loss per unit mass of water this course is a minimum. The parameters representing the morphological characteristics of 13 tributaries in the Geum River system such as stream bifurcation ratio and stream concavity were Computed from the Horton-Strahler's laws and are used to check the law of average stream fall. The result showed that the law of average stream fall agrees reasonably well with law of Horton-Strahler. Concavity of a river basin is shown to be the determinative factor to the formation of a stream system. Concavity of a river basin is shown to be the determinative factor to the formation of a stream system. Based on Horton's Law and the law of average stream fall, longitudinal stream profiles can be calculated.

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Insecticidal Activity of Ethyl Acetate Extracts from Culture Filtrates of Mangrove Fungal Endophytes

  • Abraham, Silva;Basukriadi, Adi;Pawiroharsono, Suyanto;Sjamsuridzal, Wellyzar
    • Mycobiology
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    • v.43 no.2
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    • pp.137-149
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    • 2015
  • In the search for novel potent fungi-derived bioactive compounds for bioinsecticide applications, crude ethyl acetate culture filtrate extracts from 110 mangrove fungal endophytes were screened for their toxicity. Toxicity tests of all extracts against brine shrimp (Artemia salina) larvae were performed. The extracts with the highest toxicity were further examined for insecticidal activity against Spodoptera litura larvae and acetylcholinesterase (AChE) inhibition activity. The results showed that the extracts of five isolates exhibited the highest toxicity to brine shrimp at 50% lethal concentration ($LC_{50}$) values of 7.45 to 10.24 ppm. These five fungal isolates that obtained from Rhizophora mucronata were identified based on sequence data analysis of the internal transcribed spacer region of rDNA as Aspergillus oryzae (strain BPPTCC 6036), Emericella nidulans (strains BPPTCC 6035 and BPPTCC 6038), A. tamarii (strain BPPTCC 6037), and A. versicolor (strain BPPTCC 6039). The mean percentage of S. litura larval mortality following topical application of the five extracts ranged from 16.7% to 43.3%. In the AChE inhibition assay, the inhibition rates of the five extracts ranged from 40.7% to 48.9%, while eserine (positive control) had an inhibition rate of 96.8%, at a concentration of 100 ppm. The extracts used were crude extracts, so their potential as sources of AChE inhibition compounds makes them likely candidates as neurotoxins. The high-performance liquid chromatography profiles of the five extracts differed, indicating variations in their chemical constituents. This study highlights the potential of culture filtrate ethyl acetate extracts of mangrove fungal endophytes as a source of new potential bioactive compounds for bioinsecticide applications.

An Analysis of Low-level Stability in the Heavy Snowfall Event Observed in the Yeongdong Region (영동지역 대설 사례의 대기 하층 안정도 분석)

  • Lee, Jin-Hwa;Eun, Seung-Hee;Kim, Byung-Gon;Han, Sang-Ok
    • Atmosphere
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    • v.22 no.2
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    • pp.209-219
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    • 2012
  • Extreme heavy snowfall episodes have been investigated in case of accumulated snowfall amount larger than 50 cm during the past ten years, in order to understand the association of low-level stability with heavy snowfall in the Yeongdong region. In general, the selected 4 events have similar synoptic setting such as the Siberian High extended to East Sea along with the Low passing by the southern Korean Peninsula, eventually inducing easterly in the Yeongdong region. Specifically moist-adiabatically neutral layer has been observed during the heavy snowfall period, which was easily identified using vertical profiles of equivalent potential temperature observed at Sokcho, whereas convective unstable layer has been formed over the East sea due to relatively warm sea surface temperature (SST) about $8{\sim}10^{\circ}C$ and lower temperature around 1~2 km above the surface, obtained from RDAPS. Difference of equivalent potential temperature between 850 hPa and surface as well as difference between air and sea temperatures altogether gradually increased before the snowfall period. Instability-induced moisture supply to the atmosphere from the East sea, being cooled and saturated by the upper cold surge, would make low-level ice cloud, and eventually move inland by the easterly flow. Heavy snowfall will be enhanced in association with low-level convergence by surface friction and upslope wind against Taebaek mountains. This study emphasizes the importance of low level stability in the Yeongdong region using the radiosonde sounding and RDAPS data, which should quantitatively be examined through numerical model as well as heat and moisture supply from the ocean.

Effect of Bark Content and Densification Temperature on The Properties of Oil Palm Trunk-Based Pellets

  • Wistara, Nyoman J;Rohmatullah, Moh Arif;Febrianto, Fauzi;Pari, Gustan;Lee, Seung-Hwan;Kim, Nam-Hun
    • Journal of the Korean Wood Science and Technology
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    • v.45 no.6
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    • pp.671-681
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    • 2017
  • Oil palm trunk (OPT) is a potential source of biomass for the production of biopellet. In the present research, biopellet were prepared from the meristem part of 25 years old OPT with various percentages of its bark (0, 10, and 30%). The highest biopellet durability was found for biopellet produced at $130^{\circ}C$ of pelletizing temperature with 30% bark content. Scanning electron microscopy (SEM) of biopellet showed the weak of particle bonding due to the low pelletizing pressure. The moisture content, unit density, ash content, and caloric value of OPT-based pellets were 3.55-5.35%, $525.56-855.23kg/m^3$, 2.76-3.44%, and 17.89-19.14 MJ/kg, respectively. The combustion profiles obtained by thermogravimetric analysis (TGA) seemed to be unaffected by the bark content on. Differential thermal analysis of TGA curve indicated different pyrolysis characteristic of hemicellulose, cellulose, and lignin.

Modeling of Depth/Width of Cut for Abrasive Water Jet Milling of Titanium (티타늄의 워터젯 밀링을 위한 가공깊이/폭 모델링)

  • Park, Seung Sub;Kim, Hwa Young;Ahn, Jung Hwan
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.25 no.1
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    • pp.83-88
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    • 2016
  • Because of the increasing tool cost for cutting hard-to-cut materials, abrasive water jet (AWJ) milling recently has been regarded as a potential alternative machining method. However, it is difficult to control the depth and width of cut in AWJ milling because they vary depending on many AWJ cutting parameters. On 27 conditions within a limited range of pressure, feed rate, and abrasive flow rate, AWJ cutting was conducted on titanium, and depth profiles were measured with a laser sensor. From the depth profile data, depth and width of cut were acquired at each condition. The relationships between depth and parameters and between width and parameters were derived through regression analysis. The former can provide proper cutting conditions and the latter the proper pick feed necessary to generate a milled surface. It is verified that pressure mostly affects depth, whereas abrasive flow rate mostly affects width.

Simplified Limit Solutions for the Inclined Load Capacity of a Dynamically Installed Pile in Soft Clay

  • Lee, Junho;Jung, Jong-Suk;Sim, Young-Jong;Park, Yong-Boo
    • Land and Housing Review
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    • v.11 no.2
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    • pp.87-94
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    • 2020
  • Offshore renewable energy resources are attractive alternatives in addressing the nation's clean energy policies because of the high demand for electricity in the coastal region. As a large portion of potential resources is in deep and farther water, economically competitive floating systems have been developed. Despite the advancement of floating technologies, the high capital cost remains a primary barrier to go ahead offshore renewable energy projects. The dynamically installed piles (DIPs) have been considered one of the most economical pile concepts due to their simple installation method, resulting in cost and time-saving. Nevertheless, applications to real fields are limited because of uncertainties and underestimated load capacity. Thus, this study suggests the appropriate analytical approach to estimate the inclined load capacity of the DIPs by using the upper bound plastic limit analysis (PLA) method. The validity of the PLA under several conditions is demonstrated through comparison to the finite element (FE) method. The PLA was performed to understand how flukes, soil profiles, and load inclinations can affect the inclined load capacity and to provide reliable evaluations of the total resistance of the DIPs. The studies show that PLA can be a useful framework for evaluating the inclined load capacity of the DIPs under undrained conditions.