• Journal of Korean Society of Water and Wastewater
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• v.35 no.2
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• pp.135-142
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• 2021

The activity of anaerobic ammonium oxidation (ANAMMOX) immobilized in synthetic media (Poly Ethylene Glycol, PEG) and granular form was evaluated comparatively to investigate the effect of influent nitrogen concentration and exposure of oxygen. In ANAMMOX granule reactor, when concentration of influent total nitrogen increased to 500mg/L, removal efficiency of ammonium, nitrite and nitrate were shown to 90.5±6.5, 96.6±4.9, and 93.2±6.1%, respectively. In the case of the PEG gel, it showed lower nitrogen removal performance, resulting in that the removal efficiency of ammonium, nitrite and nitrate were shown to 83.3±13.0, 96.4±6.1, and 90.3±7.5%, respectively. In second step, when exposed to oxygen, the nitrogen removal performance in the ANAMMOX granule reactor also remained stable, but the activity of PEG gel ANAMMOX was found to be inhibited. Consequently, the PEG gel ANAMMOX was a higher sensitivity than that of granular ANAMMOX with two variables applied in this study.

• Journal of Animal Environmental Science
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• v.14 no.2
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• pp.113-118
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• 2008

Sequencing batch operation consists of fill, react, settle and decant phases in the same reactor. Operation consists of anaerobic, anoxic and oxic (aerobic) phases when nutrient removal from the wastewater is desired. Since the same reactor is used for biological oxidation (or mixing) and sedimentation in aerobic and anaerobic SBR operations, capital and operating costs are lower than conventional activated sludge process and conventional anaerobic digestion process, respectively. Therefore, Aerobic SBR and Anaerobic SBR operations may be more advantageous far treatment of small volume animal wastewater in rural areas.

• Journal of Microbiology and Biotechnology
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• v.24 no.4
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• pp.534-544
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• 2014

The effects of microbial iron reduction and oxidation on the immobilization and mobilization of copper were investigated in a high concentration of sulfate with synthesized Fe(III) minerals and red earth soils rich in amorphous Fe (hydr)oxides. Batch microcosm experiments showed that red earth soil inoculated with subsurface sediments had a faster Fe(III) bioreduction rate than pure amorphous Fe(III) minerals and resulted in quicker immobilization of Cu in the aqueous fraction. Coinciding with the decrease of aqueous Cu, $SO_4{^{2-}}$ in the inoculated red earth soil decreased acutely after incubation. The shift in the microbial community composite in the inoculated soil was analyzed through denaturing gradient gel electrophoresis. Results revealed the potential cooperative effect of microbial Fe(III) reduction and sulfate reduction on copper immobilization. After exposure to air for 144 h, more than 50% of the immobilized Cu was remobilized from the anaerobic matrices; aqueous sulfate increased significantly. Sequential extraction analysis demonstrated that the organic matter/sulfide-bound Cu increased by 52% after anaerobic incubation relative to the abiotic treatment but decreased by 32% after oxidation, indicating the generation and oxidation of Cu-sulfide coprecipitates in the inoculated red earth soil. These findings suggest that the immobilization of copper could be enhanced by mediating microbial Fe(III) reduction with sulfate reduction under anaerobic conditions. The findings have an important implication for bioremediation in Cu-contaminated and Fe-rich soils, especially in acid-mine-drainage-affected sites.

• Environmental Engineering Research
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• v.11 no.4
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• pp.173-180
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• 2006

Autotrophic nitrogen removal and its microbial community from a laboratory scale upflow anaerobic sludge bed reactor were characterized with dynamic behavior of nitrogen removal and sequencing result of molecular technique (DNA extraction, PCR and amplification of 16S rDNA), respectively. In the experiment treating inorganic wastewater, the anaerobic granular sludge from a full-scale UASB reactor treating industrial wastewater was inoculated as seed biomass. The operating results revealed that an addition of hydroxylamine would result in lithoautotrophic ammonium oxidation to nitrite/nitrate, and also hydrazine would play an important role for the success of sustainable nitrogen removal process. Total N and ammonium removal of 48% and 92% was observed, corresponding to nitrogen conversion of 0.023 g N/L-d. The reddish brown-colored granular sludge with a diameter of $1{\sim}2\;mm$ was observed at the lower part of sludge bed. The microbial characterization suggests that an anoxic ammonium oxidizer and an anoxic denitrifying autotrophic nitrifier contribute mainly to the nitrogen removal in the reactor. The results revealed the feasibility on development of high performance lithoautotrophic nitrogen removal process with its microbial granulation.

• Journal of Korean Society on Water Environment
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• v.22 no.3
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• pp.482-491
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• 2006

The anaerobic ammonium oxidation (ANAMMOX) from substrates with various $NO_2-N$ and $NH_4-N$ concentationes, which were generated from piggery waste was accomplished by using anaerobic granular sludge as seeding sludge. As the result of operation, when $NO_2-N/NH_4-N$ ratios of ANAMMOX influent were 0.6~1.5, $NO_2-N/NH_4-N$ removal ratios were exhibited 1.19~2.07 (average 1.63). The higher influent $NO_2-N/NH_4-N$ ratios resulted in higher $NO_2-N/NH_4-N$ removal ratios by ANAMMOX. It means that $NO_2-N$ concentration is very important factor in ANAMMOX. Specific ammonium removal rate was constantly as $0.03{\sim}0.04gNH_4-N/g$ VSS-day at $35^{\circ}C$ while it was $0.01gNH_4-N/g$ VSS-day at $20{\sim}30^{\circ}C$. Thus, in order to reduce the effluent N concentration, either an increase of ANAMMOX reactor HRT or more biomass accumulation at the optimal temperature can be considered.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.7
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• pp.700-704
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• 2008

Nitrite and free ammonia have been known as substrate inhibitors in anaerobic ammonium oxidation. To reduce inhibitory effect of these substrates, the NH$_3$-N/NO$_2$-N ratio in the influent could be properly controlled in anaerobic ammonium oxidation process. Five kinds of NH$_3$-N/NO$_2$-N ratio were assayed in batch to find optimum NH$_3$-N/NO$_2$-N ratio, curtailing substrate inhibition. As the results of batch test, the highest T-N removal efficiency of 88% was obtained at 1.00 : 1.30 of NH$_3$-N/NO$_2$-N ratio. In addition, rate constants for ammonium and nitrite in zero-order kinetics were found to be the highest values as 7.66 mg/L$\cdot$hr and 11.89 mg/L$\cdot$hr at 1.00 : 1.30 ratio, respectively. However, as for the specific anammox activity, the ratio of NH$_3$-N/NO$_2$-N ratio was recommended as 1 : 1.15 which can maintain the highest SAA during continuous operation and preclude the accumulation of nitrite in the reactor.

• Journal of Korean Society on Water Environment
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• v.36 no.3
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• pp.220-228
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• 2020

Anaerobic ammonium oxidation (AMX) is a cost-efficient biological nitrogen removal process. The coexistence of ammonia-oxidizing bacteria (AOB) in an AMX reactor is an interesting research topic as a nitrogen-related bacterial consortium. In this study, a sequencing batch reactor for AMX (AMX-SBR) was operated with a conventional activated sludge. The AOB in an AMX bioreactor were identified and quantified using terminal restriction fragment length polymorphism (T-RFLP) and real-time qPCR. A T-RFLP assay based on the ammonia monooxygenase subunit A (amoA) gene sequences showed the presence of Nitrosomonas europaea-like AOB in the AMX-SBR. A phylogenetic tree based on the sequenced amoA gene showed that AOB were affiliated with the Nitrosomonas europaea/mobilis cluster. Throughout the enrichment period, the AOB population was stable with predominant Nitrosomonas europaea-like AOB. Two OTUs of amoA_SBR_JJY_20 (FJ577843) and amoA_SBR_JJY_9 (FJ577849) are similar to the clones from AMX-related environments. Real-time qPCR was used to quantify AOB populations over time. Interestingly, the exponential growth of AOB populations was observed during the substrate inhibition of the AMX bacteria. The specific growth rate of AOB under anaerobic conditions was only 0.111 d^-1. The growth property of Nitrosomonas europaea-like AOB may provide fundamental information about the metabolic relationship between the AMX bacteria and AOB.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.4
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• pp.607-628
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• 1999

The swine waste industry is growing rapidly along with the world human population. The trend is toward more concentrated piggeries with numbers of herds in the thousands. Associated with these increased herds are large quantities of wastes, including organic matter, inorganic nutrients, and gaseous emissions. The trend in swine waste management is toward treatment of these wastes to minimize negative impact on the health and comfort of workers and animals and the atmosphere, water, and soil environments. Treatment of these wastes has traditionally involved land application, lagoons, oxidation ditches, and conventional batch and continuously stirred reactor designs. More sophisticated treatment systems are being implemented, involving advanced anaerobic digester designs, integrated with solids separation, aerobic polishing of digester effluents, and biological nutrient removal. This review discusses the present and future role of anaerobic processes in piggery waste treatment with emphasis on reactor design, operating and performance parameters, and effluent processing.

• Journal of Korean Society for Atmospheric Environment
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• v.27 no.6
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• pp.703-710
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• 2011

Odor emission from domestic sewer systems has become a serious environmental problem. An investigation on a sewer manhole revealed that anaerobic decay of sediment organic matters (SOMs) and related declines of oxidation reduction potential (ORP) in the sediment layer were the main reason of the production of volatile sulfur compounds. In addition, as the anaerobic decaying period continued, the odor intensity rapidly increased with increasing concentrations of $H_2S$ and dimethyl sulfide. As a feasible method to control SOMs and to minimize odor emission potentials, an electrolytic oxidation process has been employed to the sediment sludge phase. In this study, voltages applied to the electrolytic oxidation process were varied as a main system parameter, and its effects on odor removal efficiencies and reaction characteristics were investigated. At the applied voltages greater than 20 V, the system efficiently oxidized the organic matter, and the ORP in the sludge phase increased rapidly. As a consequence, the removal efficiency of hydrogen sulfide was found to be >99% within 60 minutes of the electrolytic oxidation. Overall, the electrolytic oxidation process can be an alternative to control odor emission from sewer systems, and a threshold input energy needs to be determined to achieve effective operation of the process.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.3
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• pp.199-204
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• 2006

The purpose of this work was to evaluate the development of the anammox process by the use of granular sludge selected from a digestion reactor as a potential seed source in a lab-scale UASB (upflow anaerobic sludge blanket) reactor system. The reactor was operated for approximately 11 months and was fed by synthetic wastewater. After 200 days of feeding with $NH_4^+\;and\;NO_2^-$ as the main substrates, the biomass showed steady signs of ammonium consumption, resulting in over 60% of ammonium nitrogen removal. This report aims to present the results and to more closely examine what occurs after the onset of anammox activity, while the previous work described the start-up experiment and the presence of anammox bacteria in the enriched community using the fluorescence in situ hybridization (FISH) technique. By the last month of operation, the consumed $NO_2^--N/NH_4^+-N$ ratio in the UASB reactor was close to 1.32, the stoichiometric ratio of the anammox reaction. The obtained results from the influent-shutdown test suggested that nitrite concentration would be one key parameter that promotes the anammox reaction during the start-up enrichment of anammox bacteria from granular sludge. During the study period, the sludge color gradually changed from black to red-brownish.