• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.139.2-140
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• 2000

No Abstract, See Full Text

• Journal of Plant Biotechnology
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• v.44 no.4
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• pp.462-471
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• 2017

Thiamine pyrophosphate (TPP), the active form of thiamine is a cofactor for enzymes involved in central metabolism pathways. However, it is also known to have a role as a stress signaling molecule in response to environmental changes. Anabaena sp. and N. oculata are microorganisms which are abundantly found in Malaysia's freshwater and marine ecosystem. However, not much studies have been done especially in regards to thiamine biosynthesis. This work aimed to amplify of gene transcripts coding for thiamine biosynthesis enzymes besides looking at the expression of thiamine biosynthesis genes upon stress application. Various stress inducers were applied to the cultures and RNA was extracted at different time points. The first two genes, ThiC and ThiG/Thi4 encoding enzymes of the pyrimidine and thiazole branch respectively in the thiamine biosynthesis pathway were identified and amplified. The expression of the genes were analysed via RT-PCR and the intensity of bands were analysed using ImageJ software. The results showed up to 4-fold increase in the expression of ThiC and ThiG gene transcript as compared to control sample in Anabaena sp. ThiC gene in N. oculata showed an expression of 6-fold higher as compared to control sample. In conclusion, stresses induced the expression of the gene coding for one of the most important enzymes in thiamine biosynthesis pathway. This is an agreement with the hypothesis that overexpression of thiamine is crucial in assisting plants to combat abiotic stresses.

• Korean Journal of Ecology and Environment
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• v.41 no.2
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• pp.264-274
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• 2008

Seasonal variations of cyanobacterial toxins (microcystins) in Yeongehun reservoir were studied from March to December, 2007. High level of microcystins production was shown during the late autumn and winter seasons. Total microcystins concentration increased sharply when the dominant species changed from Anabaena sp. to Microcystis aeruginosa. Microcystins-RR and -YR were the main components of the microcystins, whereas microcystin-LR was detected in small quantities. Especially, large amounts of microcystin-LR were detected when standing crops of M. aeruginosa increased exponentially. Total microcystins concentration showed a negative correlation with water temperature. However, total microcystins were lowly correlated with other environmental factors except for water temperature. As a result, this study clearly demonstrated that M. aeruginosa was the main producer of microcystins in Yeongchun reservoir.

• Korean Journal of Microbiology
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• v.37 no.1
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• pp.15-20
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• 2001

In this study, we have isolated bacterial cell wall lytic enzyme in the culture supernatant of Aspergillus sp. HCLF-4. This hydrolase showed cell wall lytic activity against Anabaena cylindrica. The extracellular enzyme was produced by Aspergillus sp. HCLF-4 when it was grown in a PDB media containing 0.05% heat killed Micrococcus luteus cells. The molecular weight of lytic enzyme was about 14.3 kDa. The optimal pH and temperature for the activity of this enzyme were 3.0~4.0 and $30^{\circ}C$, respectively. This hydrolase activity was reduced by $Na^{+}$, $Li^{+}$, $Ca^{2+}$, $Cu^{2+}$, $Fe^{3+}$, EDTA, and PMSF, whereas it was increased by $Mg^{2+}$, $Mn^{2+}$>. The enzyme has N-acetylmuramyl-L-amidase or endopeptidase activity.

• Current Research on Agriculture and Life Sciences
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• v.17
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• pp.45-52
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• 1999

This study was conducted to evaluate the effects of microbial inoculation on sewage sludge composting. The number and species of microorganisms in sewage sludge sampled on February were higher than those sampled on August. The composting of sewage sludge is inhibited by the polyacrylamide cation, which was used as a coagulant and known to repress the growth of microorganisms. The growth of all microorganisms was inhibited by the addition of the polyacrylamide cation at a concentration of more than 0.8%. The species and viable counts of microorganisms were observed to increase during composting sewage sludge by inoculation of the effective microorganisms and addition of the pine tree sawdust as a bulking agent, compared with those without inoculation. A variety of organisms in compost(sewage sludge plus sawdust) were observed after composting for 30 days, such as Fragilaria sp., Proales sp., Vorticella sp., Schizothrix sp., Anabaena sp., Zoothaminium sp., Epstylis sp., Arcella sp., Balantidium sp., Actinophrys sp., Synedra sp., Euglypha sp., Ulothrix sp., Anacystis sp., and Clostium sp.

• Journal of Korean Society of Environmental Engineers
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• v.34 no.11
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• pp.780-785
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• 2012

Existing states of attached algae in the sedimentation basin was observed during late april to early october, and the attached algae were visible 7 days after of cleaning the trough and the communities of algae became larger with increasing the operation periods. Attached algae community included bluegreen algae (Oscillatoria sp.), diatom (Synedra sp.,) and green algae (Mougeotia sp., Oedogonium sp.) and suspended diatom (Stephanodiscus sp.) as well. Diatom (Cymbella sp., Navicula sp., Synedra sp. and Stephanodiscus sp.), green algae(Mougeotia sp. and Cosmarium sp.) and blue-green algae (Anabaena sp.) were detected in the effluent of sedimentation basin. The chlorophyll-a (chl-a)concentrations of algae community on a square centimeter after 14 and 28 days were distinctively different depended on the copper sulphate treatment. The concentration of chl-a were $4{\mu}g/L/cm^2$ and $19{\mu}g/L/cm^2$ for the copper sulphate treated water and $59{\mu}g/L/cm^2$ and $147{\mu}g/L/cm^2$ for the untreated water. Diatom algae fragments were observed in red-brownish sediments on the bottom of industrial water distribution basin and degraded blue-green and green algae formated organic sediments combined with oxidized iron.

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2002.05a
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• pp.73-73
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• 2002

본 연구는 2001년 9, 10, 11월에 2주 간격으로 6개 정점에서 한강 하류 수욕6개 정점(팔당대교-행주대교)에서 식물플랑크톤 군집과 수지로한경(수온, 염분도, pH, DO, BOD, SS, TN, TP, $NO_{3}-N$, $NH_{4}-N$, $PO_{4}-P$)을 조사하였다. 수온은 7,8월에 $24~27^{\circ}C$ 로 높았으며, BOD의 평균 농도는 정점 1과 2에서 각각 1.26mg/l, i.39mg/l이었고, 정점 3~6에서 3.52~4.01mg/l이었다. $NO_{3}-N$은 정점 1에서 6까지 평균 1.36~1.98mg/l의 범위였고, $PO_{4}-P$의 평균 농도는 BOD, $NO_{3}-N$와 마찬가지로 하류오 향할수록 높아져 정점 6에서 정점 1의 39배였다. TN은 평균 1.74~6.89mg/l, TP는 0.03~0.42mg/l의 범위였다. 한강하류의 수질은 강우에 영향이 크며, 탄천의 영향을 받는 정점 3(영동대교)에서 급격히 나빠져 성산대교까지 유지되다가 안양천의 영향을 받는 행주대교에서 최고조로 악화되는 거으로 파악되었다. 식물플랑크톤 현존량은 6월 4일 평균 $5.81\times10^{6}cells/l$로 감소하였다가 7월 13일 $28.53\times10^{6}cells/l$로 증가하였다. 7월 28일과 8월 11일에 각각 $3.07~8.34\times10^{6}cells/l$로 감소하였다가 8월25일 $24.47\times10^{6}cells/l$로 다시 증가하여 조사기간 중 항시 대발생이 유지되고 있었다. 본 수역에서 우점종은, 6월에 전 조사정점에서 Aulacoseria granulata와 Aulacoseria granulata var. angustussima, 7월에는 Aulacoseria granulata var. angustussima와 Stephanodiscus hantzschii f. tenuis와 남조류인 Anabaena sp., Oscillatoria sp.가 우점을 하였다. 8월에는 남조류인 Anabaena sp. Microcystic sp. Osillatoria sp.가 높은 비율로 우점하였다. 여름철 식물플랑크톤 대발생에 영향은 수온과 직산염이 중요하였으나, 부유물질 크게 기여하지 못하였다.

• Proceedings of the Zoological Society Korea Conference
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• 1996.10a
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• pp.91.2-91
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• 1996

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.133.1-133
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• 1997

No Abstract, See Full Text

• ALGAE
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• v.19 no.2
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• pp.145-148
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• 2004

As a result of the 2-year monthly monitoring of the phytoplankton community at 3 stations in Mankyeong Estuary, Korea, we learned that cyan bacterial species of the genus Anabaena occurred at most sampling points with huge salinity differences (0.1-32.5 psu). We isolated several clones of Anabaena spp. from the monitoring stations, and screen out two euryhaline and nitrogen-fixing Anabaena clones, CB-MAL21 and CB-MAL22. The two clones were grown under various environmental gradients such as temperature (20, 30, 35 and 40$^{\circ}C$), salinity (0, 2, 5, 15 and 30psu), and $PO_4^{3-}$-P concentration (0, 1.6, 8.0, 40 and 200 ${\mu}M$M). Growth of CB-MAL21 and CB-MAL22 was measured by daily monitoring of chlorophyll fluorescence from each experimental culture for more than three serial transfers. Both the two experimental clones did not grow at 0psu. Maximal growth rates of the two clones were markedly reduced at lower $PO_4^{3-}$-P concentrations showing negligible growth at 0 and 1.6 ${\mu}M$M. However, growth of CB-MAL21 was not affected by low $NO_3^--$ concentration in culture media, showing the nitrogen-fixing ability. Maximum biomass yields of the two clones decreased dramatically at 35 and 40$^{\circ}C$. Optimal growth conditions for the two experimental clones were determined to be 20-30$^{\circ}C$, 40 ${\mu}M$M $PO_4^{3-}$-P, and wide salinity range from 5.0 to over 30psu. Best growth of CB-MAL21 was shown at (20$^{\circ}C$-15psu), which is less saline and cooler condition than those (i.e., 30$^{\circ}C$-30psu) for the best growth of CB-MAL22. The euryhaline and nitrogen-fixing CB-MAL21 strain thus can be a candidate laboratory culture for the future cyan bacterial marine biotechnology in temperate coastal waters.